Insemination Research Articles

Donor sperm: only for the rich and straight

Although single women and lesbian couples are given access to donor spermatozoa, all kinds of restrictions limit their actual use of donor insemination. These restrictions are frequently justified by the increase in safety (lowering the genetic risk) or well-being of donor-conceived persons. However, these restrictions directly result in stratified reproduction in which only the rich (those with the necessary financial means) and the straight (those who conform to dominant views of parenthood) are allowed to reproduce.

Psychological health among adult offspring of lesbian parents at a time of anti-LGBTQ+ oppression

ABSTRACT This study investigates potential differences in psychological health (i.e. life satisfaction, anticipated future life satisfaction, distress), social well-being, and experiences of victimisation among adult offspring born to lesbian parents when compared with participants from a nationally representative sample surveyed in the same historical context of renewed anti-LGBTQ+ oppression. The research involved 75 adult offspring (M = 30.93, SD = 0.93; sex assigned at birth: 39 female and 36 male; gender identity: 73 cisgender and 2 gender nonbinary; sexual orientation: 51 heterosexual and 24 sexual minority) of the first generation conceived through donor insemination by lesbian parents. These offspring were matched with 75 participants of a U.S. probability sample based on several factors, including age, race/ethnicity, education, sex assigned at birth, gender identity, and sexual orientation. The comparison group is presumed to have been raised by heterosexual parents. After applying Bonferroni’s correction to minimise the risk of Type I errors resulting from multiple comparisons, no significant differences emerged in the study variables between the two samples. Despite a climate of renewed anti-LGBTQ+ oppression, adult offspring of lesbian parents were doing well, possibly due to unique socialisation processes in childhood when their parents taught them to be resilient even in the face of socio-environmental challenges.

O-181 It takes more than genes to build a family

Abstract Sperm donation has been known to mankind possibly for a very long time, although first medical reports come from the late 18th century. Initially, most inseminations in married couples were homologous, involving the husband’s semen. The first publicized breakthrough that came in 1884 would today be considered unethical, as an anesthetized woman was subjected to an injection of semen from a handsome (as duly reported) student of medicine, without any consent of both her and her spouse. The veil of secrecy that covered heterologous insemination was finally lifted when Dr Mary Barton openly acknowledged the practice in her clinic and published the first manual on “artificial insemination.” It set the ethical standards for such procedures—including mutual anonymity of donors and recipients and maintaining absolute secrecy of the fact of donor insemination—for decades to come. The cost was of course the lost identity of the conceived children and the often reported emotional detachment of the fathers from their social, but not genetical, offspring. The grave consequences of the limited pool of donors were yet to be discovered with the advent of DNA testing. In the UK, Barton’s husband and another collaborator were confirmed to father at least 100 children each. In the Netherlands, the country was shaken by the case of Jan Karbaat who used the sperm of an anonymous donor—who probably should not have even passed psychological screening—to bring more than 200 babies to the world. Suddenly, lots of adults learnt of the existence of their never expected siblings. Concomitantly, more and more parents revealed the fact of donor conception to their children motivated by openness. The secrets could not be held any longer and Joanna Rose was the first British woman that won a court case in 2002 against her own country that was wilfully breaking her right to respect for private and family life guaranteed by European Convention on Human Rights. The proponents of open donation found support also in Convention on the Rights of the Child. More and more countries started enforcing either open or at least double-track donation from then on. However, even more legislations blissfully ignore these ethical developments and maintain complete anonymity of the donor insemination procedures. This is where my personal story begins, as a father of twins born in 2012 from in vitro fertilization thanks to the semen of an anonymous donor. At that time, Poland even did not have an actual law on fertility treatment. The ART centres adopted their own policies on anonymous donation and signed contracts with their patients on a case by case basis. The legal status in my country was further cemented with the long overdue adoption in 2015 of Act on Fertility Treatment that forbids non-anonymous donation and even excludes the prospective parents from the decision process on the choice of the donor. The burden of partially lost identity of my kids will be with me possibly my entire life. But I don’t want it to cast a shadow on our family relations. We have to deal with my non-genetic fatherhood one way or another. We maintain our family ties in full knowledge of the fact of the existence of some unnamed benefactor that made this possible. Maybe one day my children will find some unsuspected genetic relatives via increasingly popular public DNA banks if they choose so, thus sticking together parts of their identity. Only time will tell.

Cumulative live birth rates following intrauterine insemination using donor and husband sperm in different age groups: Acohort study of 10 415 insemination cycles.

To assess the age-specific cumulative live birth rates (CLBRs) in intrauterine insemination (IUI) cycles using either donor or husband sperm, and to investigate the impact of sperm sources on IUI success among women within the same age group. This retrospective cohort study comprised women who underwent IUI with donor sperm (IUI-D) or husband sperm (IUI-H) from 2017 to 2021. The women were stratified based on their age at the initiation of insemination into four categories: <35, 35-37, 38-39 and ≥40 years. A total of 5253 women undergoing 10 415 insemination cycles (3354 with IUI-D and 7061 with IUI-H) were included. The CLBRs decreased significantly with increasing maternal age within donor and husband insemination groups (P < 0.001). In the IUI-D group, the crude CLBRs were 61.50% in women aged <35, 48.91% in 35-37, 24.14% in 38-39 and 11.76% in the ≥40-year age category, respectively. The corresponding rates in the IUI-H group were 27.62%, 22.96%, 13.73% and 6.90%, respectively. Within the <35 and 35-37-year age categories, the CLBRs were significantly higher following IUI-D cycles compared to IUI-H cycles, with hazard ratios (HR) of 1.85 (1.68-2.04) and 1.69 (1.16-2.47), respectively. However, within the 38-39 and ≥40-year age categories, both IUI-D and IUI-H resulted in comparable low CLBRs, with HRs of 1.91 (0.77-4.76) and 1.80 (0.33-9.86), respectively. Advanced maternal age affects the whole process of fertility. Therefore, it could be reasonable to limit the number of IUI performed in women aged 40 years and older, even in couple using donor sperm for reproduction.

Parental burnout, coparenting, and internalized sexual stigma during COVID-19 in parents with minoritized sexual identities.

The COVID-19 pandemic acted as a model of stressful situations for parents insofar as it led to unprecedented difficulties in childcare and caregiving, resulting in increased levels of parental burnout, worldwide. To date, research on parental burnout has mainly involved heterosexual parents. However, parents with minoritized sexual identities face partially different stressors, including internalized sexual stigma, and they also have partially different resources, including a more egalitarian division of childcare labor. Between April 2020 and February 2021, 32 lesbian mother families by donor insemination (n = 64 lesbian mothers) and 28 gay father families by gestational surrogacy (n = 56 gay fathers), all with a child aged 6-10 years and living in Italy, were recruited. In each family, both parents self-rated their parental burnout, coparenting, and internalized sexual stigma. Multilevel modeling indicated that lesbian mothers reported greater parental burnout than gay fathers. Moreover, lower coparenting quality was associated with greater parental burnout. Finally, internalized sexual stigma had a significant both direct and interactive effect on parental burnout, with higher levels of internalized sexual stigma resulting in greater parental burnout, especially in gay fathers. Considering the sexual minority stress theory and the risks and resources balance theory, the results indicate the importance of preventing and treating parental burnout in lesbian and gay parents by focusing on their internalized sexual stigma and coparenting relationship. Also, incorporating the positive psychology framework in future research would help identify in these parents the resources deriving from their minoritized sexual identities to deal with parental burnout. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Parenting, dyadic coping, and child emotion regulation in lesbian, gay, and heterosexual parent families through assisted reproduction

ABSTRACT Aims The present study examined the associations between family structure, parenting, and dyadic coping and children’s emotion regulation in lesbian, gay, and heterosexual parent families through assisted reproduction. It also explored differences in parenting dimensions and dyadic coping, based on parents’ sexual orientation. Methods Participants were 60 lesbian mothers through donor insemination, 50 gay fathers through surrogacy, and 42 heterosexual parents through gamete donation, all with a child aged 6–12 years (M = 8.67; SD = 2.16; 48.68% females) and residing in Italy. In each family, both parents reported parenting stress, parent – child relational self-efficacy, parental control, dyadic coping, and their perception of the child’s emotion regulation. Results Mixed models indicated no significant differences between family types in parenting stress and use of a controlling parenting style. However, lesbian mothers reported higher parent – child relational self-efficacy than heterosexual parents, and higher dyadic coping strategies than gay fathers. Also, children of gay fathers showed greater emotion regulation than children of heterosexual parents. Across family types, higher levels of parenting stress and dyadic coping, and lower levels of parent – child relational self-efficacy were associated with lower child emotion regulation. Conclusion Given that in middle childhood, across family types, better emotional regulation was associated with lower parenting stress and dyadic coping, and greater effectiveness in the parent – child relationship clinical work should focus on the parent’s and couple’s ability to cooperatively manage stressors while maintaining a balanced focus on their children’s resources and autonomy.

Study on the Effect of Bee Strain and Insemination Method on the Hygienic Behavior of Bee Colonies Tested Using the Pricking Method

The process of artificial and natural insemination was carried out on the queen bees of the local and Carniolan strains in order to study their hygienic behavior and the Pricking method for the strains, as well as the cross-insemination results between them. The study results showed that the local honey bee strain T1 outperformed the other studied strains in its hygienic behavior, as the detection rate of the dead brood by Pricking was (44.16, 93.16, and 100) % respectively, after (1, 6, and 24) hours of conducting the study. The same strain also outperformed all other strains in removing the dead brood with a percentage ranging from (0.16, 22.66, and 100) % respectively, after (1, 6, and 24) hours of conducting the study. Moreover, the artificial insemination method generally outperformed the natural insemination method in the hygienic behavior of the bee colonies, as the detection rate of the brood in the artificially inseminated bee strains A2 was (40.91, 91.00, 97.33, and 100) % respectively, after (1, 6, 24, and 36) hours of conducting the study. A2 also outperformed the A1 insemination method in terms of the percentage of removing the dead brood by Pricking, with a percentage ranging from (0.00, 17.08, 96.91, and 100) % respectively, after (1, 6, 24, and 36) hours of conducting the study.

The Clinical Embryologist: Past, Present and Future Challenges

Embryologist is an essential part of infertility care defined as a highly skilled and well-trained person who can work in teamwork to execute continuous tasks with near-zero error and pay attention to detail with excellent clinical judgment. At the very beginning, embryologist’s routine daily tasks were quite simple including processing the sperm and eggs for natural insemination. Following that, advanced infertility treatment emerged quickly including intracytoplasmic sperm injection (ICSI), vitrification-warming, embryo shipping, intracytoplasmic morphologically sperm injection (IMSI), as well as trophectoderm biopsy as a source of chromosomal copy number testing or monogenic disorders analysis. Collectively, the implementation of such improved treatments renders complexity in the IVF laboratory that could be simplified in the future. Apparently, the implementation of advanced technology in the field of IVF nowadays has two main purposes. The first is to improve the IVF success rate including finding precise markers for embryo selection such as genomics, proteomics, and metabolomics fingerprint and images-based artificial intelligence algorithm for embryo selection. The second is to standardize or simplify routine daily tasks in the embryology laboratory by implementing automated software such as for laboratory quality control maintenance, a microfluidic system that allows standardization of handling gametes, micro-robotic for immobilizing sperm for ICSI, fully automated semen analysis, electronic tagging of laboratory devices to improve traceability. All such automated systems will serve as advanced clinical decision tools to boost IVF success rate and infertility care quality. An elevated automated system in the future in an embryology laboratory would impact the clinical embryologist in terms of reduced quantity and conversely, increased qualities of highly scientific embryologists with proper education. Embryologist roles may change from conventional routine daily tasks to well-trained and experienced embryologists who are able to scientifically evaluate the effectiveness of certain approaches or potential clinical use of such implementation in their clinical laboratory setting.

Study on the Effect of Bee Strain and Pollination Method on the Hygienic Behavior of Honey Bee Colonies Tested by Liquid Nitrogen Freezing Method

Bee queen breeding was conducted for local and Carniolan honey bee strains, as well as hybrids resulting from cross-pollination, using both natural and artificial insemination methods. The hygienic behavior test was conducted using liquid nitrogen freezing method. The results showed that the hybrid bee strain T3 outperformed with detection and removal rates of 92.66% and 87.50% respectively, 48 hours after the study, regardless of the insemination method. The results also demonstrated the superiority of the natural insemination method, regardless of the studied strain, with detection and removal rates of 85.66% and 78.66% respectively, 48 hours after the killing process. The results also showed the interaction between the bee strain and the insemination method, 48 hours after the killing process, with significant superiority for treatment T2A1 in terms of detection and removal rates at 98.00% and 96.66% respectively. It was followed by treatment T3A2, which had no significant difference in detection rate (94.33%) and a removal rate of 89.00%, which was not significantly different from treatment T3A1 with a removal rate of 86.00%.

The motives and experiences of donor-conceived persons requesting the identity of their sperm donors

Research questionWhat are the motives and experiences of donor-conceived persons (DCP) who search for and receive information about their identity-release sperm donor? DesignA qualitative interview study with 29 individuals (21 women, seven men, one non-binary) who were consecutively recruited after having requested information about their sperm donor at five Swedish University hospitals. All participants were conceived after donor insemination to heterosexual couples within an identity-release donation programme. Individual semi-structured interviews were conducted face-to-face or via telephone between September 2016 and November 2019, and transcribed audio recordings were analysed using reflexive thematic analysis. ResultsThe motives and experiences of DCP were described in two themes. The theme ‘donor information can fill different needs’ describes that varying motives, thoughts and feelings are related to searching for and obtaining donor information. Motives ranged from curiosity and a desire for agency over one's conception to hopes of finding a new father. The theme ‘navigating donor information in a relational context’ describes the process of obtaining donor information as interpersonal, highlighting that the DCP needs to balance the interests of different stakeholders, and that obtaining donor information can challenge the relationship quality with the father. ConclusionsObtaining the donor's identity has the potential to affect the understanding of DCP of themselves, and to influence relationships within their family in unexpected and challenging ways. Therefore, adequate resources should be allocated to support the growing number of families after identity-release donation.

Donor sibling relations among adult offspring conceived via insemination by lesbian parents.

How do adult offspring in planned lesbian-parent families feel about and relate to their donor (half) sibling(s) (DS)? A majority of offspring had found DS and maintained good ongoing relationships, and all offspring (regardless of whether a DS had been identified) were satisfied with their knowledge of and contact level with the DS. The first generation of donor insemination offspring of intended lesbian-parent families is now in their 30s. Coincident with this is an increased use of DNA testing and genetic ancestry websites, facilitating the discovery of donor siblings from a common sperm donor. Few studies of offspring and their DS include sexual minority parent (SMP) families, and only sparse data separately analyze the offspring of SMP families or extend the analyses to established adult offspring. This cohort study included 75 adult offspring, longitudinally followed since conception in lesbian-parent families. Quantitative analyses were performed from online surveys of the offspring in the seventh wave of the 36-year study, with a 90% family retention rate. The data were collected from March 2021 to November 2022. Participants were 30- to 33-year-old donor insemination offspring whose lesbian parents enrolled in a US prospective longitudinal study when these offspring were conceived. Offspring who knew of a DS were asked about their numbers found, characteristics or motivations for meeting, DS terminology, relationship quality and maintenance, and impact of the DS contact on others. All offspring (with or without known DS) were asked about the importance of knowing if they have DS and their terminology, satisfaction with information about DS, and feelings about future contact. Of offspring, 53% (n = 40) had found DS in modest numbers, via a DS or sperm bank registry in 45% of cases, and most of these offspring had made contact. The offspring had their meeting motivations fulfilled, viewed the DS as acquaintances more often than siblings or friends, and maintained good relationships via meetings, social media, and cell phone communication. They disclosed their DS meetings to most relatives with neutral impact. The offspring, whether with known or unknown DS, felt neutral about the importance of knowing if they had DS, were satisfied with what they knew (or did not know) of the DS, and were satisfied with their current level of DS contact. This study is the largest, longest-running longitudinal study of intended lesbian-parent families and their offspring, and due to its prospective nature, is not biased by over-sampling offspring who were already satisfied with their DS. The sample was from the USA, and mostly White, highly educated individuals, not representative of the diversity of donor insemination offspring of lesbian-parent families. While about half of the offspring found out about DS, the other half did not. Regardless of knowing of a DS, these adult offspring of lesbian parents were satisfied with their level of DS contact. Early disclosure and identity formation about being donor-conceived in a lesbian-parent family may distinguish these study participants from donor insemination offspring and adoptees in the general population, who may be more compelled to seek genetic relatives. The study participants who sought DS mostly found a modest number of them, in contrast to reports in studies that have found large numbers of DS. This may be because one-third of study offspring had donors known to the families since conception, who may have been less likely to participate in commercial sperm banking or internet donation sites, where quotas are difficult to enforce or nonexistent. The study results have implications for anyone considering gamete donation, gamete donors, donor-conceived offspring, and/or gamete banks, as well as the medical and public policy professionals who advise them. No funding was provided for this project. The authors have no competing interests. N/A.

Adults Conceived via Donor Insemination by Lesbian Parents Reflect on Their Own Future Parenting Plans (and Their Own Parents Reflect on Being Grandparents)

The National Longitudinal Lesbian Family Study (NLLFS) started in 1986 when donor insemination became available for lesbian women in the U.S. Wave 7 of the NLLFS was the first time that adults conceived via donor insemination were asked if they had or planned to have children, and what they anticipated telling children about their own nontraditional conception. Of 75 NLLFS adult offspring (mean age 30.93; 49.33% female, 48.00% male, and 2.66% gender nonbinary; 90.67% White, 9.33% people of color), 8 (10.67%) had children and 42 of the rest (62.67%) hoped to have children. Most anticipated no parenting challenges, though some mentioned societal reactions or not having had a father. They expected to rear children in an open-minded and child-focused way, and to discuss their own nontraditional conception in a casual and straightforward manner. They viewed their own mothers as role models. Wave 7 was also the first time that NLLFS parents were asked about grandchildren; of 124 parents, 7 (5.64%) were grandparents. They expressed joy in spending time with their grandchildren and pride in their offspring’s parenting skills. The results are discussed in relation to research about how parents who are members of minority groups educate children about minority status.

Risk Factors Linked with Preeclampsia: A Review

Preeclampsia is a condition that affects pregnant women and is characterized by high blood pressure, protein in the urine, and swelling in the hands, feet, and legs. There is a chance for mild to significant variances. Although it can happen earlier or immediately after delivery, it usually happens in the later stages of pregnancy. Indeed, search engines like Google, Microsoft Bing, and Baidu were used to search publications investigating the association between pre-eclampsia and risk factor. It was observed that if preeclampsia occurred during a prior pregnancy, a woman is seven times more likely to suffer it again. Primarily, initial pregnancies are affected. Preeclampsia is more likely to affect women with history of migraines, gestational diabetes, diabetes, rheumatoid arthritis, lupus, scleroderma, urinary tract infections, gum disease, polycystic ovarian syndrome, multiple sclerosis, and sickle cell disease. Additionally, it happens more commonly in pregnancies brought on by egg donation, in vitro fertilization, or donor insemination. It is recommended that high-risk pregnant women begin taking low-dose aspirin after 12 weeks to prevent preeclampsia. Preeclampsia is therefore x-rayed along with its signs and causes. There are several risk indicators that, alone or in combination, may help identify early pregnant women who are “high risk” for pre-eclampsia. Keywords: High blood pressure, Preeclampsia, Risk factors.

P-059 Sperm DNA fragmentation (SDF) after cryopreservation and sperm selection: implications for clinical pregnancies and live births after intrauterine insemination with donor sperm

Abstract Study question Can SDF assessment pre-cryopreservation, post-thaw and after post-thaw sperm selection predict clinical outcome in a heterologous intra-uterine donor insemination program (IUI)? Summary answer SDF increased post-cryopreservation in donor sperm and after post-thaw density gradient without affecting clinical pregnancy, live birth and miscarriage rates. What is known already Sperm cryopreservation is effective for preservation of male fertility and facilitates the storage of donor semen, while infectious disease screening can be completed and confirmed negative. Sperm viability and motility are the most vulnerable parameters during the freeze-thaw process. Inherent reactive oxygen species may induce SDF via oxidative stress. Controversial results are reported regarding induction of SDF after cryopreservation due to differences in cryo tolerance, methods of cryopreservation and methods used to evaluate SDF. Sperm selection post-thaw is indispensable to select a normal motile fraction for insemination. Again, results are controversial regarding influence on SDF levels after sperm selection post-thaw. Study design, size, duration This was a prospective, observational study. Between March 2015 and March 2019, eighteen potential sperm donors were screened via a three step plan – semen assessment, medical assessment and serological blood tests. In accordance with the Belgian legislation (2007), a sperm donor was matched to obtain a maximum of 6 pregnancies in acceptor women/couples, allowing for more than one offspring/woman or couple. A total of 106 acceptors were matched for heterologous intra-uterine insemination. Participants/materials, setting, methods Semen samples were collected after 2-7 days abstinence and standard semen parameters analyzed within 60 mins after ejaculation. Within the same time frame SDF test was performed using TUNEL assay both before and after cryopreservation and after a two-step discontinuous density gradient centrifugation post-thaw. Samples were cryopreserved by the conventional slow freezing method using sperm freeze solution. A yield of ≥ 2 M progressive spermatozoa post-thaw after selection was sufficient to contemplate IUI. Main results and the role of chance Mean female age at first cycle was 33.9 ± 4.1 years and donor age 28.5 ± 5.6 years. Out of 429 cycles, 100 (23.3%) resulted in clinical pregnancy. Excluding three ongoing pregnancies, we counted 75 live births (17.6% of cycles or 77.3% of pregnancies), while 21 pregnancies ended in miscarriage (4.9% of cycles or 21.6% of pregnancies) and 1 resulted in stillbirth. Progressive motility (59.3 ± 12.5%) decreased significantly after cryopreservation (30.0 ± 13.2%; p &amp;lt; 0.001), but increased post-thaw after density gradient centrifugation (61.6 ± 16.0%; p &amp;lt; 0.001). On the contrary, SDF (12.0 ± 5.9%) increased post-cryopreservation (26.3 ± 14.5%; p &amp;lt; 0.001) and further increased after sperm selection (34.9 ± 22.1%; p = 0.04). Using multilevel mixed-effects logistic regression (Odds ratio [95%CI]), we found that female age significantly influenced clinical pregnancy (0.911 [0.847-0.981]; p = 0.01), live birth (0.894 [0.834-0.959]; p = 0.002) and miscarriage rates (1.180 [1.033-1.347]; p = 0.015). There was no significant age-adjusted effect of SDF post-thaw after density gradient selection on clinical pregnancy (1.007 [0.994-1.021]; p = 0.30), live birth (1.001 [0.988-1.014]; p = 0.84) or miscarriage (1.021 [0.997-1.046]; p = 0.08). Median time-to-live birth was 4 cycles. Analyses of time-to-live birth with mixed-effects Cox models revealed no significant association with progressive motility; SDF before or after cryopreservation and after sperm selection post-thaw. Limitations, reasons for caution Results concern a small donor population, with high normal semen parameters and should therefore be extrapolated cautiously to subfertile men with normal and subnormal semen parameters. Wider implications of the findings In a donor IUI program with strict selection on conventional sperm parameters, differences in SDF before and after cryopreservation and gradient centrifugation do not change IUI outcomes, although cryopreservation and centrifugation increase SDF. Findings could be useful for the optimization of sperm freezing and selection in different assisted reproductive procedures. Trial registration number not applicable

Выбор оптимального времени и методы искусственного осеменения собак

We collected, analyzed, and summarized information on the optimal timing and methods of artificial insemination of dogs at present. Scientific articles published in the bibliographic databases such as e-library, Scopus, and ResearchGate were used for this review. It has been established that artificial insemination has significant advantages over natural insemination, including reliable long-term storage of sperm in cryobanks, the possibility of exporting and importing genetic material without transporting animals, and obtaining offspring from elite male producers from anywhere in the world, even after their death.&#x0D; The time of artificial insemination and natural mating is determined by the date of ovulation using direct (ultrasound visualization of the ovaries) or indirect methods (picture of vaginal smears, multiple measurements of blood progesterone concentration, etc.). In dogs, the egg cell ovulates immature at the stage of the oocyte of the first order. For this reason, it is recommended to inseminate them on the 2nd… 5th days after ovulation, when egg maturation ends in the fallopian tube - the formation of a second-order oocyte.&#x0D; For dog insemination, freshly obtained, diluted chilled, and cryopreserved sperm are used. When using freshly obtained and diluted cooled sperm, the method of choice is an intra-vaginal method of sperm administration, and frozen-thawed sperm requires transcervical intrauterine insemination under video endoscopic control.

Seroprevalence and risk factors associated with bovine viral diarrhoea virus in Turkey.

Bovine viral diarrhoea virus (BVDV) is an infectious agent that is extensively observed worldwide and is among the crucial pathogens of the cattle industry. BVDV infection causes gastrointestinal and respiratory diseases. This study aimed to determine the seropositivity and associated potential risk factors for the risk assessment of BVDV infection in the dairy cattle population in the Aegean Region of Turkey. In this cross-sectional study, 552 serum samples were collected between August 2018 and September 2019 from 48 herds that were not vaccinated against BVDV. Farmers were interviewed using a questionnaire on potential BVDV risk factors. BVDV-specific antibodies in serum samples were detected using a commercial indirect-ELISA kit. The herd-level and animal-level prevalence of BVDV seropositivity was 89.58% (95% CI: 77.83-95.47) and 48.37% (95% CI: 44.23-52.54), respectively. The multivariable logistic regression model analysis demonstrated a positive correlation between BVDV infection and age, common pasture use with small ruminants, the use of natural insemination, and history of respiratory and/or reproduction problems. The results of this study showed that BVDV infection is common in dairy herds in the Aegean Region. The risk assessment would aid the implementation of a voluntary control programme for BVDV in this region.

Perspectives of Youth With LGBTQ+ Parents: Feelings of Openness and Acceptance Toward Others, Oneself, and Family

Youth with LGBTQ+ parents may feel that their parents’ LGBTQ+ identity impacted their lives because of unique experiences such as LGBTQ+ family socialization. Guided by family systems, queer, and social constructionism theories, we explored this question via in-depth interviews with 49 youth (8 with minoritized gender identities and 25 with minoritized sexual identities) between 12 and 25 years old ( Mage = 19.51, SD = 3.48) in the United States, and who had at least one LGBTQ+ parent. Using inductive thematic analysis, four themes were identified: (1) feelings of openness and acceptance toward the self; (2) feelings of openness and acceptance toward others; (3) feelings of openness and acceptance toward their family; and (4) perceptions of normalcy. Many endorsed these themes and reported that they felt this way specifically due to their parents’ LGBTQ+ identities. Youth were asked about their thoughts related to future families. These themes were considered in relation to their family formation (e.g., adoption; donor insemination). This research indicates that youth with LGBTQ+ parents may experience unique socialization about openness and acceptance in the context of their families. This research is important in informing culturally competent practice for other youth, parents, and those who serve LGBTQ+ parent families.

Accessing publicly funded donor insemination treatment in the UK: is funding information available on fertility clinic websites?

We sought to find out if information about public funding for regulated donor insemination (DI) was available on UK fertility clinic websites, and if so, what information was provided for same-sex couples and single women; and if the available information was easily readable. The ‘Choose a fertility clinic’ pages of the Human Fertilisation and Embryology Authority (HFEA) website were used to identify all licensed fertility clinics in the UK, and any available text on public funding for DI treatment was extracted. The Flesch reading ease scores were calculated to determine the readability of the extracted text. Of the 52 clinics included in the synthesis, 23 mentioned public funding, and for 16 of these, it was unclear whether public funding was available. Six of the 23 clinics mentioned public funding for same-sex couples, and two mentioned public funding for single women. The Flesch reading ease scores indicated that none of the text about funding for DI treatment on any of the clinic websites met the NHS-advised level of readability for health information. Fertility clinic websites should specify whether they offer publicly funded treatment, and to whom, as well as clearly stating the eligibility criteria, using suitably readable language to communicate this.

The influence of expanded carrier screening in assisted reproductive techniques: changed the 'game'- review.

Expanded carrier screening constitutes a new scientific tool able to detect conditions that can be treated immediately after birth or during pregnancy. Its implementation could affect both the prenatal period and assisted reproductive techniques. It is strongly beneficial as it provides much useful information to future parents concerning the medical status of their offspring. In addition, the definition of 'serious/severe', regulating preimplantation diagnosis, donor insemination, and even the definitions of prerequisites for abortion diseases, should be reformed including all clinically severe diseases. On the other hand, controversies may arise especially regarding gamete donation. Future parents and offspring maybe informed regarding donors' demographic and medical characteristics. This study aims to investigate the effects of the implementation of expanded carrier screening in the reformation of the definition of 'severe/serious' disease, the decision-making of future parents, gamete donation, and the possible new moral dilemmas that may arise.

Relationships between mothers and children in families formed by shared biological motherhood.

Does shared biological motherhood, in which a woman gives birth to the genetic child of her female partner, result in more positive mother-child relationships than donor insemination, in which only one mother is biologically related to the child? Mothers in both family types showed high levels of bonding with their children and viewed their relationship with their child positively. There is some evidence of feelings of inequality regarding their relationship with their child between biological and non-biological mothers in lesbian mother families formed by donor insemination, with a qualitative longitudinal study showing a tendency for children to form stronger bonds with their biological than their non-biological mother. Thirty lesbian mother families created through shared biological motherhood were compared with 30 lesbian mother families formed by donor-IVF. All families had two mothers who both participated in the study, and the children were aged from infancy up to 8 years old. Data collection took place over 20 months beginning in December 2019. Each mother in the family was interviewed separately using the Parent Development Interview (PDI), a reliable and valid measure of the nature of the parent's emotional bond with their child. The interviews were transcribed verbatim and coded separately by one of two trained researchers who were unaware of the child's family type. The interview produces 13 variables that relate to the parent's representations of themselves as a parent, 5 variables that relate to the parent's representations of the child, and a global variable that assesses the extent to which the parent can reflect on the child and their relationship. Families formed through shared biological parenthood did not differ from families created by donor-IVF in terms of the quality of mothers' relationships with their children as assessed by the PDI. Neither were differences identified between birth mothers and non-birth mothers across the entire sample, or between gestational and genetic mothers within the families formed by shared biological parenthood. Multivariate analyses were conducted to minimize the role of chance. Ideally, larger samples of families and a narrower age range of children would have been studied, but this was not possible as we were reliant on the small number of families formed through shared biological motherhood in the UK when the study began. To maintain the anonymity of the families, it was not possible to request information from the clinic that may have shed light on differences between those who responded to the request to participate and those who did not. The findings show that shared biological motherhood is a positive option for lesbian couples who wish to have a more equal biological relationship to their children. One type of biological connection does not appear to have a greater influence on the quality of parent-child relationships than the other. This study was funded by the Economic and Social Research Council (ESRC) grant ES/S001611/1. KA is Director, and NM is Medical Director, of the London Women's Clinic. The remaining authors have no conflicts of interest to declare. N/A.