Insect Food Research Articles

Effect of photoperiod and 20-hydroxyecdysone on post-embryonic development and diapause disruption in Cirina butyrospermi Vuillet (Lepidoptera: Saturniidae) under laboratory conditions

Abstract Cirina butyrospermi (Lepidoptera: Saturniidae), commonly known as the shea caterpillar, is an edible insect in several West African countries. However, this insect is seasonal due to a long pupal diapause. This study therefore aims to explore the potential for mass rearing of C. butyrospermi by determining the role of photoperiod and 20-hydroxyecdysone in disrupting or shortening diapause. Exposure of larval growth to different photoperiod conditions, i.e. L.D. 6:18, L.D. 8:16, L.D. 10:14 and L.D. 12:12, affected larval weight and adult emergence. With respect to the photoperiod treatments applied to larval growth from neonate to fourth instar, caterpillars reared under L.D. 6:18 conditions weighed more than those reared under the other photoperiods. However, photoperiod treatments did not significantly affect the pupation rate. Adult emergence was only observed under L.D. 6:18 photoperiod conditions for a diapause average duration of 140.85 days. The recorded emergence percentage was 38.46 ± 1.33%, and emergences occurred over a period of ten days. When the L.D. 6:18 photoperiod conditions were applied only on the fourth instar larvae, 24.39 ± 1.41% of pupae developed to adults in 34.50 days on average and emergences were grouped in 4 days. Injections of increasing doses of 20-hydroxyecdysone into diapausing pupae resulted in diapause breaking within 23-28 days in 7-47% of treated pupae, depending on the dose of 20-hydroxyecdysone and the pupal weight. Conversely, diapause remained in non-treated pupae during the study period. Short photoperiod and 20-hydroxyecdysone are key drivers for diapause termination in C. butyrospermi. The possible interaction between 20E doses and treated pupae weight provides new insights into the hormone’s efficacy in breaking pupal diapause in C. butyrospermi. While the results need further clarification, they represent a significant step towards mass breeding of this important food insect.

Development of Indirect Sandwich ELLA for Detection of Insects in Food

Development of Indirect Sandwich ELLA for Detection of Insects in Food

Can Sustainable Food from Edible Insects Become the Food of the Future? Exploring Poland’s Generation Z

This study addresses a research gap by examining the attitudes and interest of young Polish consumers in new foods containing insects. The results of the survey, which are presented in this article, were obtained as part of an inter-university project conducted at five Polish higher education institutions (N = 1063). The survey was conducted using the indirect interview method via an online platform (CAWI). This study aimed to assess attitudes, behaviours and intentions to purchasing insect foods, as well as factors influencing the selection of new foods containing insects and product preferences for such foods. The analysis revealed no statistically significant differentiation in consumer behaviour and declared intentions towards insect foods based on gender or place of residence (p > 0.001). Our results revealed correlations between the level of neophobia and negative attitudes among young consumers towards insect products, albeit weak in nature. The exploratory factor analysis suggested that the assessment of factors influencing the purchase of insect products may be based on a small number of dimensions, which were not found to be correlated, as follows: “Health and Environmental Concern”, “Organoleptic Attributes” and “Circumstantial Influences”.

Clinical Characteristics and Some Related Factors in Children with Prurigo Nodularis in Vietnam

BACKGROUND: Prurigo nodularis in children can become chronic, lasting from several months to many years, triggered and sustained by environmental allergens. In Vietnam, prurigo nodularis is relatively common; however, few studies have been conducted on pediatric populations. AIMS: This study aims to describe the clinical characteristics and related factors of prurigo nodularis in children. METHODS: This prospective, cross-sectional descriptive study was conducted at the National Hospital of Dermatology and Venereology from December 2023 to June 2024. A total of 117 children participated in the study. Data were collected according to the study’s medical record template. RESULTS: The results showed that the proportion of male patients was higher than that of female patients (62.4% vs. 37.6%), with the highest proportion of cases in the under-6 age group (47%). Most patients lived in urban areas (88%). Nearly half of the cases had disease duration of < 12 months (42.2%). The onset rate on the legs was the highest (58.2%). Some related factors were herbal baths (1.7%), food allergies (8.5%), pet keeping (22.2%), insect bites (23.1%), family history of prurigo (24.8%), long fingernails (59.8%), and self-medication (73.5%). The rate of regular deworming was 93.2% of the children. Most patients experienced severe itching (97.4%), with the severity and activity level of the disease mainly at moderate levels (96.6% and 97.4%, respectively). CONCLUSION: Prurigo nodularis in children presents with diverse clinical characteristics. Factors such as food allergies, pet keeping, insect bites, and family history may play a role in triggering and maintaining the disease, but further research is needed.

The unpaved road towards efficient selective breeding in insects for food and feed—A review

The unpaved road towards efficient selective breeding in insects for food and feed—A review

Promoting insects as a food delicacy - A qualitative study from the perspectives of consumers and producers in Italy

A protein shift in our diet is required due to the growing human population and the planet's limited resources. Edible insects may contribute to this shift and the achievement of Sustainable Development Goals (SDGs). The EU is addressing the issue of edible insects for the European market through a series of regulations aiming to introduce them in a safe and secure way. However, many potential consumers find it difficult to regard insects as food. This study aims to explore the factors that would promote the perception of insects as food delicacies and to understand the main obstacles to, and positive aspects of, the consumption of edible insects, using the concept of culinary identity in relation to Italian food culture. Italian food culture is often conveyed by the Made-in-Italy concept, which is appreciated worldwide. This is a qualitative study in which insects were explored as a food delicacy using Italian food culture as a tool. Stakeholders of interest were involved: Italian producers of insect-based foods and potential Italian young adult consumers. The results show that three interconnected themes coexist when placing insects as food in the market: sustainability, commerce and acceptance. At the center of these three themes, which describe the forces impacting the inclusion of edible insects in our food system, is the need to choose a target group of active consumers. Italian food culture could, therefore, be used to create insect food delicacies to motivate potential consumers to actively choose insects as food.

Consumer sensory profiling and liking of Bolognese-type sauces: how do insect and plant foods really fare against red meat?

Abstract Meeting global targets for healthier and more sustainable diets calls for a substantial reduction of meat consumption in Western nations, especially red and processed meat. This requires a transition to the large-scale production, marketing, and adoption of alternative proteins. The current state of development of new plant- and insect-based foods holds good promise, but optimizing their sensory quality to the point where they can satisfactorily replace everyday meat-eating experiences remains a challenge, demanding a more consumer-oriented approach. This study investigated how Portuguese adults (, 18-40 years old, 50% female, regular meat eaters) perceived the sensory characteristics of seven Bolognese-style pasta sauces – made with 100% red meat (beef and pork), plant (soya beans or peas) or edible insect (house cricket or yellow mealworm larvae) protein – and how this affected their hedonic preferences for these products. Expectedly, the meat-based sauce was generally the most appreciated. Still, consumers liked all products slightly to moderately apart from mealworm mince. Sensory-mediated disgust affected the evaluations of some insect- and plant-based sauces, while others benefitted from formulations that enhanced savouriness and the prototypical attributes of a tomato pasta sauce. Moreover, the disconfirmation of positive sensory-affective judgements of ‘meatiness’ induced by mince-like texture cues penalized liking. High inter-individual variation in evaluations was observed. Distinct preferences for spiciness, for instance, moderated the effects of meat substitution on product liking, with over half of participants appreciating a mild or a spicy plant-based option more than the typical beef Bolognese. Sociodemographics, Beef Consumption, Healthy Eating Consciousness and Food Disgust Sensitivity were all important predictors of patterns of product liking, pointing out that the type of protein selected is just one of the many factors driving the acceptance of meat substitutes by consumers that can be leveraged by the food industry.

Rank correlation between atopic comorbidity and laboratory parameters in allergic rhinitis

One of the current cutting-edge problems in allergy and ENT is the study of comorbidity in allergic rhinitis, which is the most common atopic disease among all other atopies. Two decades ago, a new endotype of the disease, local allergic rhinitis, was discovered. In the past, some patients with typical complaints of chronic nasal congestion, persistent runny nose, and other similar symptoms, did not display such characteristic diagnostic criteria as positive allergy skin tests and an increased value of serum total IgE and specific IgE antibodies. They were qualified by allergists and ENT specialists as individualists with non-allergic rhinitis and, accordingly, did not receive adequate treatment. However, their symptoms remained, prompting them to abuse decongestants and resulting in an increased burden on the cardiovascular system. This, along with the burden of concomitant pathology, including other atopies, can be considered a significant medical and social problem. With the purpose to study rank correlation between comorbidity and three laboratory biomarkers, 46 patients with allergic rhinitis were examined. The list of parameters included two routine items such as serum total IgE and eosinophilic cationic protein, and rarely studied IL-4 in skin exudate. Clinical analysis showed that significantly more often allergic rhinitis in patients was combined with bronchial asthma (in 39.1%, p 0.05), less often with food allergy (in 19.6%), atopic dermatitis (in 10.9%), insect allergy (in 8.7%), and allergic urticaria (in 4.5%). In one case, local perennial rhinitis was identified, accompanied by conventional seasonal rhinitis with a high content of serum total IgE and positive allergic skin tests with pollen allergens. Skin tests with household allergens, including house dust mite products, showed a negative result. This case was regarded as a manifestation of the so-called “double” allergic rhinitis. The Spearman correlation demonstrated a negative mean strength relationship between comorbidity and serum IgE and eosinophilic cationic protein (p 0.05). The seemingly paradoxical result can be interpreted quite logically, taking into account immunopathogenetic mechanisms of allergic inflammation. In particular, with an increase in the frequency of atopic comorbidity, the migration of eosinophils from the blood to sites of eosinophilic inflammation increases.

Acceptance of insects as food in Germany: risk–benefit perceptions and the role of risk preferences

PurposeWith a growing world population and climate crises, edible insects are a promising alternative to conventional livestock. However, Western consumers are largely opposed to eating insects. The aim of this study is to investigate the acceptance of insects in a German sample with a specific focus on risk–benefit perceptions and risk preferences.Design/methodology/approachData were collected via an online survey (N = 569). Established scales for food neophobia (FN), risk perception, risk preference and acceptance were used. The statistical analyses included mean comparisons regarding acceptance and risk–benefit perceptions of unprocessed and processed insects. We further examined the role of perceived benefits and risks as well as risk preferences as determinants of acceptance via hierarchical multiple regressions.FindingsRespondents indicated lower perceived risks, and higher perceived benefits and thus higher acceptance for processed insect products as compared to whole insects. Risk perception was the strongest predictor of acceptance of unprocessed as well as processed insect food. Respondents who reported to have already eaten insects before exhibited higher risk preferences than those who had not.Originality/valueThis study specifically considered the role of risk preferences, which has been so far largely overlooked in consumer research on insect foods. The findings indicate that acceptance of insect food is mainly driven by risk perception rather than individual risk preferences. However, risk preferences should not be totally overlooked as they seem to play a role especially for unprocessed insects and the decision to try insects for the first time.

Quantification of the thermal history of insects in foods using real-time PCR-based DNA fragmentation analysis

A major challenge faced by the food industry is the contamination of food by insects. Once an insect is found in a food product, it is important to determine how it entered the food. Given that the thermal history of insects provides a clue to unraveling this mystery, in this study, we have developed a novel real-time PCR-based DNA fragmentation analysis method to quantify the thermal history of insects found in foods.We selected two species of cockroaches (Periplaneta fuliginosa and Blattela germanica) as investigative targets because cockroach contamination often causes significant economic losses in the food industry. A set of real-time PCRs was designed for each species to amplify the species-specific regions of 18S ribosomal DNA at approximate 100 and 300 bp. The degree of DNA fragmentation was evaluated using the differences between the amplification curves for the 100- and 300-bp sequences. We observed that the degree of DNA fragmentation was quantifiable when the insect was heated to >100 °C. Therefore, we determined whether cockroaches contaminate retort foods during the manufacturing or household consumption stages. This novel analytical method is expected to improve food safety and product reliability by identifying the cause of insect contamination.

Intensive Care for Anaphylaxis in Children: Current Trends

Anaphylaxis is an acute allergic reaction with rapid clinical development and risk of death. This article provides an analysis of literary sources devoted to intensive care of anaphylaxis in childhood. It was revealed that the prevalence of anaphylaxis is increasing, in children and adolescents as well. The main triggers of anaphylaxis in pediatric practice include food products, insect bites and drugs. Anaphylaxis is an IgE-mediated hypersensitivity reaction of type 1, characterized by the release of chemical mediators that lead to smooth muscle contraction, increased permeability and vasodilation and vagal activation. Clinically, anaphylaxis is manifested by allergic skin rash, angioedema, obstruction of the upper respiratory tract (URT), broncho-obstructive syndrome (BOS), arterial hypotension, tachycardia. Intensive care for anaphylaxis in children begins with stabilization of the condition: stopping the entry of a possible allergen into the body, hospitalization in the anesthesiology and intensive care unit, restoring patency of the URT, conducting oxygen therapy, monitoring vital functions. The first-line drug for the treatment of anaphylaxis in children is adrenaline at a dose of 0.01 mg/kg, which stops all the main pathophysiological links of anaphylaxis. Adrenaline autoinjectors are not used in the Russian Federation. Second-line drugs for intensive care for anaphylaxis in children include glucocorticosteroids (GCS), antihistamines, bronchodilators and infusion therapy. The effectiveness of GCS in anaphylaxis in children has not been proven, antihistamines can relieve skin manifestations of an allergic reaction, but do not affect vital disorders. Bronchodilators reduce BOS and are an additional treatment, while infusion therapy eliminates hypovolemia. Knowledge and timely implementation of modern care algorithms for anaphylaxis in children and adolescents will improve the quality of emergency care and reduce the risk of fatal outcomes in this pathology.

Insect-Based Foods: A Preliminary Qualitative Study Exploring Factors Affecting Acceptance and New Product Development Ideas through Focus Groups

While insect-based foods could contribute towards sustainable protein consumption and production, their successful adoption relies largely on consumer acceptance. This case study aims to explore factors affecting acceptance and to identify the insect-based foods that participants would like to be developed and introduced to the market. Three focus groups incorporating optional tasting sessions of insect-based foods were conducted in Ireland, a country where these foods were unavailable at the time of the study. Findings showed that before the tasting sessions, public opinion, portion size comparisons (insects vs. meat), and witnessing entomophagy-related disturbing experiences through television were some of the factors that negatively influenced acceptance. Lack of availability in Ireland was the main factor that hindered consumption among participants willing to consume these foods. Participants’ usual food choice motives, sensory attributes, and inclusion percentages of insects in food were some factors which affected acceptance following the tasting sessions. A variety of insect-based foods was suggested for product development, which were mostly components of a main meal. The findings of this study could give an initial indication of product ideas that could be explored further for product development in regions where insect-based foods are not readily available on the market. These findings could contribute to future studies that would aim to build quantitative tools.

Introducing menus of three weekly insect- or plant-based dinner meals slightly reduced meat consumption in Danish families: Results of a randomized intervention study

The environmental concerns associated with excess meat consumption have emphasized the need for sustainable alternatives. Edible insects offer a promising alternative due to their environmental efficiency and nutritious profile, but their widespread adoption in Western diets remains a challenge. The objective of the study was to investigate the impact of exposing families (parents and children) to insect-based or plant-based dinner menus on dietary pattern, meat intake, and protein intake over a six-week intervention period. The study was a two-arm randomized equivalence trial comparing an insect-based menu to a plant-based control. Families received either an insect or plant-based menu to replace meat in dinner meals three times a week for six weeks, aiming to replace 20% of their meat protein intake. Dietary changes were assessed through dietary registrations and daily questionnaires. Both adults and children maintained their estimated daily total protein intake, while reducing daily meat protein intake. Neither group met the 20% weekly meat replacement goal. In the insect-based menu group, adults and children reached an average 5.5% and 2.3% weekly meat replacement, respectively. In the plant-based menu group, adults and children replaced 9.0% and 4.3%, respectively. Meat attachment had an effect on meat protein intake. The menus slightly reduced meat protein intake. The weekly frequency of meat meals slightly declined, but portions remained the same. By enhancing insect and plant-based food quality and understanding consumer behavior, insect- and plant-based products have the potential to be a complementary alternative in a sustainable dietary transition without sacrificing nutrition. Clinical trial registryClinicalTrials.gov: NCT05156853; clinicaltrials. gov/study/NCT05156853.

Trophic stoichiometry of macroelements and metals in a terrestrial food web

In order to understand the transfer of macroelements and toxic metals in the terrestrial food web, barn swallows, terrestrial frogs, and insects were collected from farmlands in the Leizhou Peninsula, and analyzed for macroelements carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) and trace metals nickel (Ni), zinc (Zn), selenium (Se), copper (Cu), chromium (Cr), cadmium (Cd), lead (Pb), and mercury (Hg). The multi-element ecological stoichiometry was discussed to trace the food web flow of nutrients and toxicants. The percentage contents of C, N, P, and S were 35.43–59.91%, 6.89–12.11%, 0.49–4.66%, and 0.44–2.19%, respectively. The concentrations of Ni, Zn, Se, Cu, Cr, Cd, Pb, and Hg were 0.163–116 mg/kg, 38.7–227 mg/kg, 0.0453–3.82 mg/kg, 3.11–141 mg/kg, not detected—79.6 mg/kg, 0.0203–0.358 mg/kg, 0.148–4.57 mg/kg, and 0.00159–1.46 mg/kg, respectively. Organisms at high trophic levels had higher contents of N, P, and S, and lower contents of C. Significant correlations were observed between δ15N and ratios of C: N, C: P, C: S, N: P, N: S, and S: P, indicating selective transfer of biogenic elements for predators in the terrestrial food web. Most metals including Ni, Zn, Se, Cu, Cr, Pb, and Hg had biomagnification factors and trophic magnification factors higher than 1, because the whole body of organisms rather than tissues were used. The negative correlations between the detoxification ratios of Se: X (each toxic metal) and metal concentrations suggest potential adverse effect of metals on terrestrial organisms.

Farmed crickets (Orthoptera: Gryllidae) raised with dermestids (Coleoptera: Dermestidae) suffer from reduced and delayed growth, but not enough to explain reports of dramatic yield loss.

The mass production of insects for food and feed is an expanding North American industry. Facilities that mass rear insects are at risk of pest infestations because the optimal environmental conditions for rearing beneficial species may also support the development of pest species. Here, we present the first recorded results detailing the interactions between dermestids and farmed crickets. Cricket farms have reported extremely low harvest yield during heavy dermestid infestations, but the exact reasons for this low yield are unknown. Many dermestid larvae are covered in dense, detachable, barbed setae called hastisetae, which are used by the larvae as an active trapping system against arthropod predators. We designed a series of experiments to test the hypotheses that a dermestid pest of cricket farms, black larder beetle (Dermestes ater DeGeer (Coleoptera: Dermestidae)), may be directly impacting Gryllodes sigillatus Walker (Orthoptera: Gryllidae) yield through the physical effects of hastisetae ingestion and/or indirectly impacting cricket yield through competition for fishmeal, a primary source of protein in conventional cricket feed. Our predictions that G. sigillatus life history and survival would be negatively affected by dermestids were largely refuted. Females fed infested diets grew less mass, but not smaller body size, compared to females fed uninfested diets. We also found that while G. sigillatus experienced delayed growth early in life after living with dermestids, they were able to tolerate living with, and consuming, dermestid larvae. We discuss how these findings have led to new hypotheses concerning how dermestid infestations drive reductions in cricket farm yield.

Optimizing Jatropha curcas bioenergy plantations in Pakistan: A geospatial suitability analysis using advanced spatial modeling

Bioenergy from energy plants, an alternative fuel, is projected to increasingly meet future worldwide energy demands as well as those of a developing nation like Pakistan. Jatropha curcas (JC) has been found to possess many desirable qualities, including high oil content seeds (27–40 %), rapid growth, ease of growing, tolerance to drought, ability to grow on poor soil and wasteland, needing less nutrient input and management, and not interfering with current food crops, insects, or pest resistance. Hence, growing JC in highly appropriate barren lands could reduce the global reliance on fossil fuels. However, an evaluation of the suitability of the land would be required for tree establishment to be effective. In this study, we integrated an Analytical Hierarchy Process (AHP) with the ArcGIS tool for assessing suitable available sites to cultivate JC across Pakistan. Using geospatial technologies, this study intended to assess and map possible areas appropriate for JC bioenergy plantations with the necessary climate, soil type, and topography factors. Pakistan was classified into 3 levels of suitability for JC plantations based on the results of a spatial analysis that integrated various data sets and varied evaluation criteria: Most Suitable, Moderately Suitable, and Less Suitable. Our results showed that out of the total area of Pakistan, around 54,392,075 hectares (57 %), 22,516,700 hectares (23 %), and 19,594,100 hectares (20 %) were identified as highly suitable, moderately suitable, and less suitable, respectively, for JC bioenergy plantations. We conclude that the methods used in this study provided a considerably reliable estimate of suitable sites for JC production in Pakistan. It can assist smallholder-based initiatives to promote JC cultivation on farmer-owned to enhance their living circumstances. Multi-criteria decision analysis (MCDA) was found to be a beneficial tool in breaking down the issues of identifying and classification of the locations for afforestation in the management of forests.

Gut metagenome in Insects and Its Potential in Agricultural and Industrial Applications

Metagenomics has greatly improved our understanding of microbial ecology by revealing the metagenomes of uncultured bacteria, including those associated with insects. These bacteria play important roles in insect defence, reproduction, and food absorption, which influences pest management tactics. Recent next generation sequencing advances in low-cost nucleotide and user-friendly bioinformatics have improved our ability to investigate microbial diversity in economically significant pests. Meta-omics technologies are now essential for defining microbial ecosystems, bio-surveillance, food safety, and commercial advances. Genome studies on insects and plants provide insights into pest control by discovering resistance genotypes and understanding stress-induced genomic changes. This review is focused on how herbivore specialists, such as the diamondback Plutella xylostella, adapt to host plants using gut microbiota. Metagenomic sequencing has discovered important bacteria-Enterobacter cloacae, Enterobacter asburiae, and Carnobacterium maltaromaticum- that help detoxify plant defences, breakdown cell walls, and synthesise amino acids. These findings indicate novel pest management tactics that target gut microbiota interactions. The paper continues with an overview of metagenomic DNA extraction, library creation and screening methods, especially on their applications in biotechnology and bioprocessing.

Analysis of the Effect Size of Insect Foods on Metabolic Syndrome-Related Indicators

Analysis of the Effect Size of Insect Foods on Metabolic Syndrome-Related Indicators

Detection Limits of Insect Fragments in Spiked Whole Wheat Flour Using Multiplex Polymerase Chain Reaction (PCR)

The need for a sensitive molecular method to detect specific species of insect contaminants in food products remains a significant challenge in the food industry. This study evaluated the detection limit of a multiplex end-point PCR assay for detecting insects in food. The assay amplifies two fragments of the cytochrome oxidase subunit I gene (COI-Fa and COI-Fb) and one fragment of the protein-coding wingless (wg) gene found in insects. Five insect species, comprising three vectors of foodborne pathogens (the housefly, Musca domestica, the American cockroach, Periplaneta americana, and the pharaoh ant, Monomorium pharaonis) and two storage insect pests (the red flour beetle, Tribolium castaneum and the Indian meal moth, Plodia interpunctella), were spiked separately and in combination at levels of 1, 0.1, 0.01, and 0.001% in whole wheat flour. At spike levels greater than 0.01%, amplicon bands of expected sizes were seen in 100% of samples containing fragments from distinct insect species. At least 25% of spiked samples at the lowest spike level had amplicon bands, except for samples spiked with M. domestica. Results showed an 18.9% probability (with 11.3% and 30% lower and upper confidence limits, respectively) of detecting insect fragments at the lowest spike level (0.001%, corresponding to 3–22 fragments), which is far below the FDA’s regulatory level of less than 75 fragments per 50 g of wheat flour. The intensity of amplicon bands in the gel images was higher at higher spike levels. However, this method is not quantitative enough to extrapolate the intensity of the amplicon bands to the number of insect fragments present in a sample. This multiplex assay was also evaluated in a variety of market food samples derived from plants and animals, showing its potential use in various food types. Overall, the sensitivity and specificity of this molecular approach suggest that it could be used in the future as a screening tool for detecting insect contaminants in food.

Innovative Foraging Behavior of Urban Birds: Use of Insect Food Provided by Cars

Despite high-quality insect food being often restricted in cities, insects are important for the development of birds. Nonetheless, plenty of insects are smashed on cars, and they are available for those species that are able to use them. We used both our own data and community science and Internet sources for surveying global, national, and local data about birds using insects on cars. Our results contained a total of 308 observations of birds collecting insects on car panels, which indicated that 39 species used this food resource since 1928 in 33 countries. Most observations considered the House Sparrow, followed by the White Wagtail and several species of corvids. European urban bird species observed to use insects on cars had a larger residual brain size. There was also some indication that bird species using insects on cars had a larger number of innovations (i.e., production of novel behaviors), greater diet generalism, and longer times living in urbanized areas than birds not observed using insects on cars. Often these species are also resident and able to use food offered in feeding sites. We assume that more bird species will use insects on cars in the future, as urban insect populations continue to decline, and thereby insects on cars will increasingly become more important sources of food for urban birds.