Insecticide Treatments Research Articles

Participatory development of practical, affordable, insecticide-treated mosquito proofing for a range of housing designs in rural southern Tanzania

BackgroundInsecticidal mosquito-proof netting screens could combine the best features of insecticide-treated nets (ITNs) and indoor residual spraying (IRS), the two most important front line vector control interventions in Africa today, and also overcome the most important limitations of these methods. This study engaged members of a rural Tanzanian community in developing and evaluating simple, affordable and scalable procedures for installing readily available screening materials on eave gaps and windows of their own houses, and then treating those screens with a widely used IRS formulation of the organophosphate insecticide pirimiphos-methyl (PM).MethodsA cohort of 54 households recruited upon consent, following which the structural features and occupant demographics of their houses were surveyed. Indoor mosquito densities were surveyed longitudinally, for approximately 3 months before and over 5 months after participatory house modification and screening using locally available materials. Each house was randomly assigned to one of three study arms: (1) No screens installed until the end of the study (negative control), (2) untreated screens installed, and (3) screened installed and then treated with PM, the insecticidal activity of which was subsequently assessed using standard cone assays.ResultsAlmost all (52) recruited households participated until the end, at which point all houses had been successfully screened. In most cases, screening was only installed after making enabling structural modifications that were accepted by the enrolled households. Compared to unscreened houses, houses with either treated or untreated screens both almost entirely excluded Anopheles arabiensis (Relative reduction (RR) ≥ 98%, P < < 0.0001), the most abundant local malaria vector. However, screens were far less effective against Culex quinquefasciatus (RR ≤ 46%, P < < 0.0001), a non-malaria vector causing considerable biting nuisance, regardless of their treatment status. While PM did not augment household level protection by screens against either mosquito species (P = 0.676 and 0.831, respectively), 8 months after treatment it still caused 73% and 89% mortality among susceptible insectary-reared Anopheles gambiae following exposures of 3 and 30 min, respectively.ConclusionsParticipatory approaches to mosquito proofing houses may be acceptable and effective, and installed screens may be suitable targets for residual insecticide treatments.

Bio-Efficacy of Chemical Insecticides Against Defoliators Spodoptera litura and Achaea janata in Castor

Field experiments on bio-efficacy of chemical insecticides against defoliators Spodoptera litura and Achaea janata in castor was carried out at Main Oilseeds Research Station, Junagadh Agricultural University, Junagadh during consecutive three year i.e. 2015-16, 2016-17 and 2017-2018. All the treatments were significantly superior over untreated check. Results of the experiment indicated that lowest number of larvae per plant for S. litura and A. janata was recorded in the treatment of chlorantraniliprole 18.5 SC 0.006% (0.42 &amp; 0.22 larvae/plant), which was at par with the most of the insecticidal treatments except the treatment of poneem after three days of the first spray. More or less similar trend was observed after 7 and 14 days of first spray as well as 3,7 and 14 days after second spray. The highest net return was recorded in treatment of indoxacarb 14.5 SC 0.0073% (Rs. 31870/-) followed by the treatment chlorantraniliprole 18.5 SC 0.006% (Rs. 31080/-), spinosad 45 SC 0.009% (Rs.29240/-) and emamectin benzoate 5 % WG 0.002 % (Rs. 26232/-). Looking to the ICBR, the treatment of profenophos 40 % + cypermethrin 4% 44 EC noted the highest ICBR i.e. (1:11.60) followed by the treatment of chlorpyriphos 20 EC 0.05% (1: 10.20), indoxacarb 14.5 SC 0.0073% (1:9.30) and emamectin benzoate 5 % WG 0.002 % (1:7.30).

Diversity of Arthropods in Relation to Insecticide and Biopesticide Treatments in Okra

Present study was carried out during 2019 at the Vegetable Research Farm, Punjab Agricultural University, Ludhiana to generate information on diversity of invertebrates in okra. Study revealed that okra crop was harboured by 27 invertebrate species belonging to 11 orders and 26 families. Of these, maximum belonged to order Hemiptera followed by Coleoptera. Higher values of Shannon-Wiener index (1.75, 1.84) and Simpson’s index (0.26, 0.17), respectively were recorded for both phytophagous arthropods and predators in untreated control plots. This is followed by neem/ other insecticides treated plots. Imidacloprid lowered the arthropod population. Occurrence of predators and phytophagous arthropods revealed a significant positive correlation with temperature (r=+0.37 to +0.99) and relative humidity (r=+0.04 to +0.80) except for few hemipterans and coleopterans.

PS-3 Effects of Feeding Rates on Diflubenzuron Efficacy Against Filth Flies (Diptera: Muscidae) in Manure of Beef Heifers

Abstract The objective of the current study is to determine the effect of feeding rates on the efficacy of diflubenzuron against filth fly development in the manure of beef heifers. Twelve Angus × Hereford crossbred heifers (263 kg ± 16.2 kg BW) were used in a completely randomized design and heifers were assigned to a 2 x 2 factorial arrangement of treatments to evaluate the efficacy of diflubenzuron against horn flies Haematobia irritans (L.), stable flies Stomoxys calcitrans and house fly Musca domestica utilizing fecal bioassays. Treatments consisted of 2 insecticidal treatments [Diflubenzuron (DFB; 0.10 mg per kg BW) and no DFB (CNT)] in a factorial arrangement with 2 dietary fed regimens [1.5% BW alfalfa hay cubes and 3.0% BW alfalfa hay cubes]. All heifers receiving DFB treatments were dosed daily beginning on day 0 and continuing through day 7. Manure samples were collected daily prior to DFB offering and for 11 consecutive days. Efficacy calculations were developed utilizing standard filth fly fecal bioassays accounting for control performance. A treatment × day interaction (P ≤ 0.01) was observed for all species largely associated with the presence of DFB and associated with the on-set of efficacy (day 0–2) and wash out (day 8–10) of the product. Feeding regimen did not (P &amp;gt; 0.05) influence DFB performance against any of the fly species evaluated. Formulations of DFB appear to handle a wide range of animal intake scenarios under controlled situations.

Does IPPM bear fruit? Evaluating reduced‐risk insecticide programmes on pests, pollinators and marketable yield

Abstract Integrated pest and pollinator management (IPPM) has been touted as a novel approach to control pests while mitigating negative effects of insecticides on pollinators. In addition to non‐chemical approaches, IPPM programmes focus on insecticide treatments with low bee toxicity, threshold‐based applications and restricted timings to avoid sprays during pollinator foraging. However, the singular and combined effect of this approach on pollination services and pest management is understudied and generally lacks field validation. In two field studies, we evaluated the effect of IPPM insecticide programmes on striped cucumber beetle (Acalymma vittatum) management, pollination and watermelon yield. The first trial (2020) featured insecticide treatments representing a factorial combination of (1) products varying in bee toxicity (bifenthrin, cyclaniliprole and acetamiprid), (2) application frequencies (weekly vs. threshold‐based) and (3) application timings (AM vs. PM), as well as an untreated control. These data were then used to inform a second trial (2021) that applied two insecticide treatments (IPPM based or grower standard) to commercial‐scale (20–30 acres) fields in collaboration with regional watermelon farms. Weekly beetle densities, pollinator visitation and yield data were collected to determine programme efficacy. Overall, beetle infestations were successfully managed with all insecticide treatments, none of which compromised marketable yield. In 2020, threshold‐based insecticide programmes offered statistically similar levels of pest control but required 20%–80% fewer applications compared to a weekly programme. Furthermore, pollinator visitation was increased in threshold‐based programmes by ~42% across all products. The on‐farm validation trial in 2021 upheld these findings; IPPM fields had effective beetle control but with 1–7 fewer insecticide applications compared to grower standard fields. IPPM fields also had 62% greater pollinator visitation and 49% more melons during harvest compared to grower standard fields. Synthesis and applications. Our study demonstrates the capacity of IPPM programmes to manage pests while mitigating negative effects on pollinators. In particular, administering insecticide applications by pest thresholds led to the greatest enhancement in pollinator function. These findings also suggest that growers can benefit from this approach with reduced insecticide costs while encouraging pollination in pollinator‐dependent crops.

Selection of Reference Genes for Normalization of qRT‒PCR Analysis in the Soybean Aphid Aphis glycines Matsumura (Hemiptera: Aphididae).

The soybean aphid Aphis glycines Matsumura is a predominant insect pest in Asia and North America and causes great losses to soybean. The release of genome data for A. glycines will facilitate gene function research in the future. However, suitable reference genes for A. glycines under various experimental conditions are scarce. To search for appropriate reference genes for A. glycines, nine candidate reference genes, including Act, α-Tub, β-Tub, RPS12, RPS18, RPL5, RPL27, EF1α, and Fer, were tested under six experimental conditions to evaluate their suitability for use in the normalization of qRT‒PCR data. Results showed that EF1α and RPS12 were optimal for the developmental stages of A. glycines, RPS18 and RPS12 were appropriate for wing dimorphism, β-Tub and RPS18 were suitable for different tissues and RPL5, and α-Tub could be used for normalization at different temperatures. β-Tub and EF1α could be proposed as reference genes for insecticide treatment, and RPL5 and RPS12 were found to be the most stable reference genes in different photoperiods. The results provide appropriate reference genes for analyzing gene expression in A. glycines and contribute to future research on the molecular physiology and biochemistry of A. glycines.

Nutritional stress exacerbates impact of a novel insecticide on solitary bees' behaviour, reproduction and survival.

Pesticide exposure and food stress are major threats to bees, but their potential synergistic impacts under field-realistic conditions remain poorly understood and are not considered in current pesticide risk assessments. We conducted a semi-field experiment to examine the single and interactive effects of the novel insecticide flupyradifurone (FPF) and nutritional stress on fitness proxies in the solitary bee Osmia bicornis. Individually marked bees were released into flight cages with monocultures of buckwheat, wild mustard or purple tansy, which were assigned to an insecticide treatment (FPF or control) in a crossed design. Nutritional stress, which was high in bees foraging on buckwheat, intermediate on wild mustard and low on purple tansy, modulated the impact of insecticide exposure. Within the first day after application of FPF, mortality of bees feeding on buckwheat was 29 times higher compared with control treatments, while mortality of FPF exposed and control bees was similar in the other two plant species. Moreover, we found negative synergistic impacts of FPF and nutritional stress on offspring production, flight activity, flight duration and flower visitation frequency. These results reveal that environmental policies and risk assessment schemes that ignore interactions among anthropogenic stressors will fail to adequately protect bees and the pollination services they provide.

Application of insecticides on peppermint (Mentha × piperita L.) induces lignin accumulation in leaves by consuming phenolic acids and thus potentially deteriorates quality

Irrational use of pesticides may lead to physiological and metabolic disorders in different crops. However, there are limited investigations on impacts of insecticides on physiology and biochemistry, secondary metabolic pathways, and associated quality of medicinal plants such as peppermint (Mentha × piperita L.). In this study, target metabolites in peppermint were monitored following foliar spraying of five insecticides: imidacloprid, pyriproxyfen, acetamiprid, chlorantraniliprole, and chlorfenapyr. Compared with the control, all insecticide treatments caused a significant loss of soluble protein (decreased by 22.3–38.7%) in peppermint leaves. Insecticides induced an increase in the levels of phytohormones jasmonic acid and abscisic acid in response to these chemical stresses. Among them, imidacloprid increased jasmonic acid by 388.3%, and pyriproxyfen increased abscisic acid by 98.8%. The contents of phenylpropanoid metabolites, including rutin, quercetin, apigenin, caffeic acid, 4-hydroxybenzoic acid, ferulic acid, syringic acid, and sinapic acid showed a decreasing trend, with pyriproxyfen decreasing the levels of quercetin and 4-hydroxybenzoic acid by 78.8% and 72.6%, respectively. Combined with correlation analysis, the content of lignin in leaves shows different degrees of negative correlations with several phenolic acids. It could be inferred that insecticides may trigger plant defense mechanisms that accumulate lignin (increased by 24.6–49.1%) in leaves by consuming phenolic acids to barricade absorption of insecticides. Through constructing networks between phytohormones and secondary metabolites, peppermint may regulate the contents of caffeic acid, 4-hydroxybenzoic acid, and sinapic acid by the antagonistic effect between salicylic acid and abscisic acid in response to insecticidal stresses. Principal component analysis and systemic cluster analysis revealed that the most pronounced changes in physiological indexes and metabolites were caused by the pyriproxyfen treatment. In conclusion, this study improves our understanding of the mechanism by which insecticides affect plant physiological and metabolic processes, thus potentially altering the quality and therapeutic value of peppermint as an example.

Transcriptome analysis reveals differential effects of beta-cypermethrin and fipronil insecticides on detoxification mechanisms in Solenopsis invicta.

Insecticide resistance poses many challenges in insect pest control, particularly in the control of destructive pests such as red imported fire ants (Solenopsis invicta). In recent years, beta-cypermethrin and fipronil have been extensively used to manage invasive ants, but their effects on resistance development in S. invicta are still unknown. To investigate resistance development, S. invicta was collected from populations in five different cities in Guangdong, China. The results showed 105.71- and 2.98-fold higher resistance against fipronil and beta-cypermethrin, respectively, in the Guangzhou population. The enzymatic activities of acetylcholinesterase, carboxylases, and glutathione S-transferases significantly increased with increasing beta-cypermethrin and fipronil concentrations. Transcriptomic analysis revealed 117 differentially expressed genes (DEGs) in the BC-ck vs. BC-30 treatments (39 upregulated and 78 downregulated), 109 DEGs in F-ck vs. F-30 (33 upregulated and 76 downregulated), and 499 DEGs in BC-30 vs. F-30 (312 upregulated and 187 downregulated). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEGs associated with insecticide resistance were significantly enriched in metabolic pathways, the AMPK signaling pathway, the insulin signaling pathway, carbon metabolism, peroxisomes, fatty acid metabolism, drug metabolism enzymes and the metabolism of xenobiotics by cytochrome P450. Furthermore, we found that DEGs important for insecticide detoxification pathways were differentially regulated under both insecticide treatments in S. invicta. Comprehensive transcriptomic data confirmed that detoxification enzymes play a significant role in insecticide detoxification and resistance development in S. invicta in Guangdong Province. Numerous identified insecticide-related genes, GO terms, and KEGG pathways indicated the resistance of S. invicta workers to both insecticides. Importantly, this transcriptome profile variability serves as a starting point for future research on insecticide risk evaluation and the molecular mechanism of insecticide detoxification in invasive red imported fire ants.

Response of cavity nesting birds to Lymantria dispar (Lepidoptera) and aerial spraying – An experimental approach

Eruptive population dynamics of forest insect species regularly attract the interest of ecologists but also often evoke debates among stakeholders concerning impacts that outbreaks or pest control activities have on forest communities including insectivorous birds. Lymantria dispar is the most serious native defoliator in mixed oak-broadleaf forests in Central Europe. To investigate the treatment effects of L. dispar density, aerial application of the insecticide Mimic (tebufenozide) and nest box accessibility for L. dispar caterpillars on nest box success of cavity nesting birds, we deployed 352 nest boxes in a full factorial experimental design with 11 replicates. L. dispar density was predicted by egg mass counts made in the year before the study. The accessibility of nest boxes for L. dispar caterpillars was manipulated in all study sites by placing nest boxes on trunks for easy access to caterpillars and on branches for more difficult access. Successful nest boxes (binary metric that combines failed breeding attempt and empty nest box) and nest productivity (number of fledged chicks in successful boxes) were monitored in the peak year of a L. dispar outbreak, during which half of the plots were treated with Mimic, as well as in the following year when L. dispar populations had widely collapsed, and no further treatments were applied. L. dispar and non-target caterpillar biomass was sampled by canopy fogging to quantify prey abundance. Caterpillar biomass was significantly reduced by Mimic in the treatment year and remained low in the post-treatment year. Despite the reduction of caterpillars, our zero-inflation model found no effect of any tested variables on nest box success of the early brood. However, in the late broods the number of successful nest boxes was reduced in Mimic treated plots in the first year by 42%, while unaffected by initial L. dispar density. The number of successful nest boxes in the second year was lower in areas with initially high L. dispar densities and there was a marginal carry-over effect of insecticide treatment. L. dispar caterpillars entered nest boxes on the trunk in higher numbers than boxes on the branch, but nest box position had no effect on nest box success of birds.We conclude that despite its strong negative impact on caterpillar prey, aerial application of Mimic targeting L. dispar caused an increase in the number of failed boxes in late broods in sprayed plots, particularly in the year of application and to some extent in the year after. We presume this delayed effect of Mimic only on late broods to be caused by Mimic-induced caterpillar shortages during first broods leading to an increased parental effort and a subsequent reduced number of second broods. The generally limited impact of Mimic might be explained by the overall extensive availability of alternate prey in oak forests in May during first broods and the ability of tits to increase their parental effort in times of caterpillar shortage. In unsprayed sites, L. dispar densities can create legacies of impact in following years, but caterpillars do not directly affect birds nest box success despite entering cavities.

Possibility of Leishmania Transmission via Lutzomyia spp. Sand Flies Within the USA and Implications for Human and Canine Autochthonous Infection.

Purpose of ReviewLeishmaniasis is a leading cause of parasitic death, with incidence rising from decreased resources to administer insecticide and anti-leishmanial treatments due to the COVID-19 pandemic. Leishmaniasis is nonendemic in the United States (U.S.), but enzootic canine populations and potentially competent vectors warrant monitoring of autochthonous disease as a fluctuating climate facilitates vector expansion. Recent studies concerning sand fly distribution and vector capacity were assessed for implications of autochthonous transmission within the U.S.Recent FindingsClimate change and insecticide resistance provide challenges in sand fly control. While most Leishmania-infected dogs in the U.S. were infected via vertical transmission or were imported, autochthonous vector-borne cases were reported. Autochthonous vector-borne human cases have been reported in four states. Further vaccine research could contribute to infection control.SummaryBoth cutaneous and visceral leishmaniasis cases in the U.S. are increasingly reported. Prevention measures including vector control and responsible animal breeding are critical to halt this zoonotic disease.

Microbe-mediated activation of indoxacarb in German cockroach (Blattella germanica L.)

The German cockroach (Blattella germanica L.) is a major urban pest worldwide and is notorious for its ability to detoxify and resist insecticides. German cockroaches have generalist feeding habits that expose them to a range of potential hazardous substances and host a wide variety of unique microbial species, which may potentially facilitate unique detoxification capabilities. Since field German cockroach populations are routinely exposed to both bait and spray insecticide treatments, we hypothesized whether these unique gut microbes might play roles in toxicological processes of the host insect. The goals of this research were to understand the metabolic processes inside the German cockroach gut after treatment with kanamycin, a broad-ranging antibiotic, and indoxacarb, an oxadiazine pro-insecticide used in cockroach bait products. In these experiments, two resistant cockroach strains were obtained from field populations in Danville, IL and compared to a susceptible laboratory strain that had no previous exposure to insecticides (J-wax). Roaches provided kanamycin-infused water had lower median mortality to indoxacarb compared to the control treatment in feeding bioassays regardless of strain, but in vial (surface contact) bioassays, only susceptible cockroaches experienced a shift in mortality apparently due to their greater susceptibility. When frass extracts of indoxacarb-fed cockroaches were analyzed, less of the active, hydrolytic metabolite DCJW (N-decarbomethoxyllated JW062) was produced relative to the parent compound indoxacarb with the antibiotic KAN. This result was further corroborated by hydrolase activity assays of whole homogenized cockroach guts. Taken together these results provide novel evidence of microbe-mediated pro-insecticide activation in the cockroach gut.

Standardized Field Trials in Cotton and Bioassays to Evaluate Resistance of Tobacco Thrips (Thysanoptera: Thripidae) to Insecticides in the Southern United States.

Foliar-applied insecticide treatments may be necessary to manage thrips in cotton (Gossypium hirsutum L.) under severe infestations or when at-planting insecticide seed treatments do not provide satisfactory protection. The most common foliar-applied insecticide is acephate. Field observations in Tennessee suggest that the performance of acephate has declined. Thus, the first objective was to perform leaf-dip bioassays to assess if tobacco thrips, Frankliniella fusca (Hinds) (Thysanoptera: Thripidae), in cotton production regions have evolved resistance to foliar-applied insecticides. A second objective was to assess the performance of commonly applied foliar insecticides for managing thrips in standardized field trials in Arkansas, Tennessee, Mississippi, and Texas. For both objectives, several insecticides were evaluated including acephate, dicrotophos, dimethoate, lambda-cyhalothrin, imidacloprid, and spinetoram. Field trials and bioassays were completed from 2018 to 2021. Dose-response bioassays with acephate were performed on tobacco thrips field populations and a susceptible laboratory population. Bioassay results suggest that tobacco thrips have developed resistance to acephate and other organophosphate insecticides; however, this resistance seems to be most severe in Arkansas, Tennessee, and the Delta region of Mississippi. Resistance to other classes of insecticides were perhaps even more evident in these bioassays. The performance of these insecticides in field trials was variable, with tobacco thrips only showing consistent signs of resistance to lambda-cyhalothrin. However, it is evident that many populations of tobacco thrips are resistant to multiple classes of insecticides. Further research is needed to determine heritability and resistance mechanism(s).

Bio-efficacy of new insecticidal molecules against plant hoppers in rice crop

The study was carried out during kharif seasons of 2020 and 2021 at different locations to assess the relative bioefficacy of new insecticidal molecules viz. Pexalon 10 SC (triflumezopyrim), Osheen 20 SG (dinotefuran), Chess 50 WG (pymetrozine) and Confidor 200 SL (imidacloprid) @ 235 ml, 500, 300 and 100 gm per hectare, respectively against plant hoppers (brown plant hopper and white backed plant hopper) in rice crop. The population of plant hoppers per hill was recorded before spray, 3, 7 and 10 days post treatment periods. It has been observed that all tested molecules are found significantly better and reduced (90.63-88.75%) the plant hoppers’ population over control. Among, insecticidal treatments Osheen 20 SG showed superiority with least plant hoppers population [(1.10, 0.55, 0.56 (av. 0.74)] per hill and it was at par with each other insecticidal molecules. The maximum grain yield (72.45 q/ha) was obtained with application of Osheen 20 SG, however the highest cost: benefit (Rs. 1: 23) was achieved with application of Confidor (100 ml/ha). It was further observed that all the tested molecules have no ill effects on spider’s population (Natural enemies).

Effectiveness of Pest Control of Cocoa Fruit (Conopomorpha cramerella as) Using Pruning and Vegetable Insecticide Treatment on Cocoa (Theobroma cacao L)

The sustainability of cocoa production in Indonesia is faced with the problem of the cocoa pod borer (Theobroma cacao L.). In an effort to control the main pests on cocoa plants, various techniques can be used, namely control with the use of natural insecticides and control by technical culture in the form of pruning. Natural insecticide is an insecticide whose basic ingredients come from plants that are easilybiodegradablein nature, so they do not pollute the environment and are relatively safe for humans and livestock, because the residue is easily lost. Pruning is the act of removing part of the plant organs in the form of branches, twigs and leaves with the aim of obtaining good cocoa plant branches, regulating the distribution of production branches and leaves so that they are evenly distributed, removing unwanted plant parts, stimulating the plants to grow. form new organs, reduce the risk of pest and disease attacks, and increase the ability of plants to form fruit. The purpose of this study was to determine the response of the application of botanical insecticides and pruning to the control of the cocoa pod borer (Theobroma cacao L.). The experimental design used a non-factorial randomized block design consisting of 7 treatments. The treatment of vegetable insecticides consisted of 4 levels, namely I0 (Without insecticide = Control), I1 (vegetable insecticide made from srikaya seeds at a dose of 250 ml/liter of water), I2 (vegetable insecticide made from tuba root with a dose of 250 ml/liter water), I3 (vegetable insecticide made from tobacco leaves at a dose of 250 ml/liter of water). The pruning treatment consisted of 3 levels, namely I4 (Without Pruning (Control), I5 (Pruning Interval 7 days after the study) and I6 (Pruning Interval 14 days after the study). Observations consisted of attack intensity, level of damage caused by PBK attack, attack index and production Control of the cocoa pod borer by pruning and botanical insecticides on cocoa plants showed a significant effect on the parameters of attack intensity and level of damage at the age of 6 weeks, and the attack index at the age of 12 weeks. 2 msp, 4 msp, 8 msp, 10 msp, 12 msp, and the parameters of the level of damage at the age of 2 msp, 4 msp, 8 msp, 10 msp, 12 msp and the plant production parameters at the age of 12 msp showed no significant effect.

Potentiality of intercropping in managing Diamondback moth (Plutella xylostella)

The present experiment was conducted at horticulture farm of Rajasthan College of Agriculture, Udaipur, Rajasthan, during two winter (rabi) seasons 2019–20 and 2020–21. The treatments include, cabbage + marigold, cabbage + onion, cabbage + garlic, cabbage + marigold + insecticides spray, cabbage + onion + insecticides spray, cabbage + garlic + insecticides spray, cabbage + insecticides spray and cabbage sole without insecticide spray as untreated control. The insecticidal treatments scheduled with two sprays, viz. first spray of chlorfenapyr 10% sc @200 g a.i./ ha and spinosad 45% sc @45 g a.i./ha. During both years, cabbage intercropped with marigold + insecticides spray proved to be the best treatment with the lowest mean DBM larval population (0.73 and 0.47 larvae/plant) and highest benefit-cost ratio (6.69 and 6.88). Additionally, the intercrops treatments, viz. cabbage + marigold, cabbage + onion and cabbage + garlic also significantly reduced the number of DBM larvae per plant as compared to the untreated sole cabbage. The different intercrops combinations evaluated had a positive effect on the cabbage crop in reducing the pest and can be taken to manage DBM in a sustainable way.

The long-term impact of an Ecohealth intervention: Entomological data suggest the interruption of Chagas disease transmission in southeastern Guatemala

Triatoma dimidiata is the main vector of Chagas disease in southern Mexico, Central America and northern South America. As a native vector, it moves readily among domestic, peri‑domestic and sylvatic environments, making it difficult to control only using insecticide as this requires regular application, and re-infestation frequently occurs. Other social innovation alternatives such as those based on Ecohealth principles can be used to tackle the dynamics of the disease in an integral way. We asked whether an Ecohealth intervention, implemented beginning in 2001 in a highly infested village, 41.8%, in southeastern Guatemala, was sustainable in the long term. This intervention included initial insecticide treatments, followed by making low-cost house improvements to eliminate transmission risk factors such as repairing cracked walls, covering dirt floors with a cement-like substance and moving domestic animals outside. We assessed the long-term sustainability through entomological and house condition surveys, as well as an analysis of community satisfaction. We found over a 19-year period, infestation with T. dimidiata was reduced to 2.2% and maintained at a level below the level (8%) where vector transmission is unlikely. This long-term maintenance of low infestation coincided with a large proportion of villagers (88.6%) improving their houses and completing other aspects of the Ecohealth approach to maintain the village at low risk for Chagas transmission. There was unanimous satisfaction among the villagers with their houses, following improvements using the Ecohealth method, which likely played a role in the long-term persistence of the modifications. Although the infestation has remained low, 11 years following the last intervention and as the population grew there has been an increase in the proportion of “at-risk” houses, to 33%, pointing out the necessity of maintaining vigilance. The Ecohealth approach is a low-cost, sustainable approach for the long-term control of vector-borne Chagas disease. We recommend this approach including ongoing community monitoring and institutional response for the long-term, integrated control of Chagas disease.

Artificial selection for emamectin benzoate resistance in the biological control agent Orius laevigatus

Biological control is occasionally supplemented with insecticides treatments, which may have a significant impact on natural enemies. Typically, selective compounds are chosen to overcome lack of compatibility, but an alternative approach is the use of biocontrol agents resistant to pesticides. Orius laevigatus (Fieber) (Hemiptera: Anthocoridae) is the main predator used to control thrips and other small pests in greenhouses. The avermectin emamectin benzoate is a bioinsecticide developed for the control of lepidopteran pests, reported as moderately to highly toxic to O. laevigatus. Firstly, we studied the variation in susceptibility to emamectin benzoate in 32 wild and commercial populations of O. laevigatus. A 62.4-fold variation in response was found (LC50 from 0.8 mg L−1 to 49.9 mg L−1). The baseline LC50 was 4.8 mg L−1. Secondly, we exploited this intraspecific variation to select four distinct emamectin-resistant strains. After 41–47 selection cycles, four resistant strains were successfully obtained (LC50 = 104–203 mg L−1) compared to the reference population (LC50 = 4.7 mg L−1). The resistance was retained for 18 generations without insecticide exposure and was expressed in all life instars, especially from the 4th nymphal instar to adult. The emamectin-resistant strains did not show cross-resistance to abamectin. Inhibitors of the detoxification enzymes failed to restore susceptibility at the concentrations tested. Fecundity and predation capacity in the resistant strain was similar to those in a commercial population. The resistance obtained may be enough to allow survival of adults and nymphs of O. laevigatus exposed to treatments of emamectin benzoate across the crop season.

Regional selection of insecticides and fungal biopesticides to control aphids and thrips and improve the forage quality of alfalfa crops

The efficacies of biological and conventional chemical insecticides against two major insect pests of alfalfa (aphids and thrips) were compared in three sites across China’s alfalfa belt. In addition, the persistence of the residues of chemical insecticides in alfalfa and their influence on the quality of alfalfa hay were examined. Efficacy varied among the different biological and chemical insecticides. The chemical insecticides were significantly more effective than biopesticides in a short time-frame. The efficacy period of biopesticides was significantly longer than that of chemical insecticides, and the corrected mortality rate of aphids in all regions was above 50% at 14 days after application. The analysis of pesticide residues showed that the residual doses of all the pesticides were within the allowed ranges after the safe period. The acid detergent fiber and neutral detergent fiber contents in alfalfa hay were higher and the protein content was lower in chemical insecticide treatments than in biopesticide treatments in Hebei. The relative feeding value of alfalfa hay treated with Metarhizium anisopliae IPP330189 was the highest among the treatments. Compared with chemical insecticides, the yield of alfalfa hay was higher in the biopesticides treatments. Biopesticides show a stronger control effect on insect populations and also a better improvement in the quality of alfalfa hay than chemical insecticides. This study provides a basis for exploring and developing a comprehensive control regime for alfalfa insect pests in the different alfalfa-growing regions in China, and for reducing chemical insecticide usage and improving forage quality.

Gut Endosymbiont Disruption through Antibiotics Influences the Metabolic Homeostasis in Spodoptera frugiperda (Lepidoptera: Noctuidae) Larvae

Fall armyworm as a polyphagous voracious feeder reported causing yield losses in most agriculturally important crops. As this insect had developed resistance against most of the insecticides, there is a need for an alternate approach to management. Gut endosymbiotic bacteria play a significant role in host feeding, digestion, and defense response throughout the life stages of insects. In the present study, we have isolated and identified the gut endosymbiotic bacteria of fall armyworm and the larvae were treated with antibiotics. The results showed that the maximum bacterial population was observed in the fourth instar of field-caught larvae and the least population was observed in the fourth instar artificially reared population. Based on the biochemical results the isolated gut endosymbiotic bacteria mainly comprised of Bacillus sp, Enterococcus sp., and Enterobacter sp. Based on the susceptibility of gut bacteria to different antibiotics, 6 antibiotic treatments with one insecticide treatment were administered to an artificial diet reared with third instar larvae and their dietary indices were evaluated. Among the antibiotic treatment, there was a reduction in the dietary indices in the larvae treated with Ciprofloxacin CIP5 (45.33%) and Cefotaxime CTX30 (41.73%) and an increase in dietary indices in the larvae treated with Nalidixin NA30 (31.58%), Doxycycline DO30 (8.82%), Vancomycin VA30 (22.05). Elimination of gut bacteria with a suitable antibiotic will affect the insect’s feeding and dietary indices subsequently decreasing the relative growth rate and insect’s physiology. Hence, gut bacteria-based green control measures might be used as an alternative approach for insecticides for the effective management of fall armyworm.