Insect Feces Research Articles

Enhancing patient care: a multimodality strategy for dilated cardiomyopathy in Chagas disease

Chagas disease is a systemic illness characterized by acute and chronic phases. If untreated, it can lead to dysfunction of vital organs, notably the heart, ultimately resulting in heart failure. Transmission primarily occurs through the feces of triatomine insects carrying the protozoan parasite Trypanosoma cruzi, either via a bite wound or intact mucous membranes. Diagnosis of Chagas disease involves serological tests, electrocardiographic findings, and imaging studies. A 58-year-old male patient from Peru with chronic dilated cardiomyopathy underwent evaluation at a tertiary care hospital. Given the uncertain etiology, a comprehensive diagnostic approach was adopted, emphasizing the pivotal role of cardiovascular magnetic resonance imaging and computed tomography angiography in managing chronic cardiomyopathy of Chagas disease. Leveraging these imaging modalities together could augment our ability to evaluate myocardial inflammation and tailor therapeutic strategies accordingly.

Utilizing Insect Frass (Faecal Matter): A Sustainable Approach to Organic Fertilization in Agriculture

Insect frass, commonly known as insect fecal matter, is emerging as a sustainable solution for organic fertilization in agriculture. This paper explores the potential benefits and applications of utilizing insect frass as an organic fertilizer, highlighting its role in promoting sustainable agricultural practices. Through a comprehensive review of literature, this study examines the nutrient composition of insect frass and its impact on soil fertility and plant growth. Furthermore, the paper discusses the ecological advantages of incorporating insect frass into agricultural systems, including its ability to reduce reliance on chemical fertilizers and minimize environmental pollution. Additionally, the study delves into various methods of utilizing insect frass in different agricultural settings, ranging from soil incorporation to foliar applications. Moreover, the paper addresses the economic feasibility of insect frass as a viable alternative to conventional fertilizers, considering factors such as production costs and market demand. By synthesizing existing research and providing insights into practical applications, this paper underscores the potential of insect frass as a sustainable approach to organic fertilization in agriculture. Ultimately, this study aims to promote further exploration and adoption of insect frass as a valuable resource for enhancing soil health, increasing crop yields, and fostering ecological sustainability in agricultural practices. As agricultural sustainability becomes increasingly imperative, innovative solutions are sought to reduce reliance on synthetic fertilizers and mitigate environmental impacts. In this pursuit, the utilization of insect frass as an organic fertilizer presents a promising avenue for sustainable agriculture. With the rapid expansion of the human population, the mass breeding of insects for feed and food has emerged as a highly efficient means of protein production. A notable byproduct of this process is insect feces, or frass, which exhibits remarkable nutrient content and abundance, surpassing animal biomass production by up to 40 times. Harnessing insect frass as an organic fertilizer offers numerous advantages, including enhanced soil fertility, improved crop yield, and reduced dependency on agrochemicals.

New insights on the spore dispersal of Phallus indusiatus s.l. (Basidiomycota, Phallaceae) for the Brazilian Amazon forest

Spore dispersal by insects (entomochory) is a crucial relationship for phalloid fungi, as mycophagous insects carry large amounts of spores and assist stinkhorns in the colonization of new sites. Phallus indusiatus s.l. is a widely distributed fungal species (Basidiomycota), which recruits generalist mycophagous insects as dispersal agents for their spores. Given the lack of knowledge about the natural history of this relationship, it is assumed that its spores are mostly dispersed by insect feces. This study was conducted in a fragment of Amazon forest in Pará, Brazil, and we (i) identified the insects that visited P. indusiatus s.l., (ii) observed the behavior of the insects during this interaction, and (iii) counted the spores carried both on the body surface and in the stomach of the visiting insects. A total of 333 insects associated with six P. indusiatus s.l. basidiomes were recorded. Stingless bees devoted the most time to foraging and were the insects that carried the largest amount of spores, >83 million spores on the body surface and >60 million in the stomach. Wasps presented low abundance, with five minutes dedicated to foraging, carrying just over 7 million spores on the body surface and >2 million in the stomach. The beetles, although possibly sheltering in the basidiomes before the total maturation of P. indusiatus s.l., were the insects with less carried spores, 189,000 spores on the body surface and about 39,000 spores in the stomach. However, they were the most abundant insects among visitors, surpassing the 100 individuals in a single basidiome. Stinkhorns are very valuable resources for forest insects and these fungi take advantage of this interaction to disperse their spores via feces, but mainly by the body surface of visitors.

Entomological indicators and food sources of triatomines in the Brazilian semi-arid region.

Triatomines are biological vectors of Trypanosoma cruzi, the etiological agent of Chagas Disease (CD) and have various mammalian hosts. This study evaluated the entomological indicators and food sources of triatomines in Petrolina in the semi-arid region of Brazil, where CD is endemic. Triatomines were captured indoors and outdoors through an active search and entomological indices (household and natural infections) were calculated. Parasitological analyses were performed through microscopic visualization using Giemsa-stained insect feces, and DNA sequencing was employed to identify food sources from the gut contents of 82 insects (9.05%) that were better preserved. We captured triatomines (906) in peridomicile (807) and intradomicile (99): Triatoma brasiliensis (84.7%, 767 specimens), Triatoma spp. (8.2%, 74 specimens), T. pseudomaculata (6.5%, 59 specimens), Rhodnius spp. (0.4%, four specimens), R. nasutus (0.1%, one specimen), and T. sordida (0.1%, one specimen). The household infestation index is 11.8%. Thirty-five triatomines were infected (33 T. brasiliensis and two T. pseudomaculata), corresponding to a natural infection index of 3.8%. The identified food sources were human T. pseudomaculata and T. brasiliensis, dogs for T. brasiliensis and rodents (Mus musculus) for T. brasiliensis. The results reinforce the need to intensify CD diagnosis, surveillance, and control actions, as an increase in entomological indices was recorded. Blood from humans and domestic and synanthropic animals was detected in the infected triatomines, suggesting a risk of CD vector transmission in Petrolina. As CD is a zoonosis, multidisciplinary and intersectoral CD surveillance must be conducted in the context of the One Health.

LC–MS/MS analysis of carcinogenic tobacco-specific nitrosamines in Spodoptera litura using the QuEChERS method

Nicotine is a highly addictive alkaloid and a neurostimulator found in tobacco that causes addiction in humans and makes tobacco a high-demand commercial product. It is popularly used for recreational purposes and is a harmful substance (Oral LD50 value for rat is 50 mg/kg) and causes addiction. The metabolites of nicotine such as the Tobacco-specific Nitrosamines (TSNAs) are hazardous substances whose metabolites are highly electrophilic and form DNA adducts, which will initiate the process of carcinogenesis. TSNAs are formed during curing, storage and fermentation due to the nitrosation of nicotine and other tobacco alkaloids. TSNAs are used as biomarkers for cancer risk assessment in humans exposed to tobacco and its products. To determine the occasional formation of TSNAs in tobacco-feeding insects, 5th instar larvae of Spodoptera litura and their faeces were analyzed for the presence of N′-nitrosonornicotine (NNN), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) along with the stored tobacco leaves (PT-76) using an Agilent 6470B LC–MS/MS system following ISO/DIS 19290:2015 protocol. The larvae are extracted in a buffered acetonitrile–water extraction and the amount of TSNAs are quantified in multiple reaction monitoring (MRM) mode. 20 upmul of each extracted and cleaned up sample was injected into the LC–MS/MS system for quantification. The Limit of Detection (LOD) and Limit of Quantification (LOQ) were 0.001 mg/kg and 0.005 mg/kg for all the tested nitrosamines. NNN was found to be 0.361 mg/kg, 0.340 mg/kg, and 5.66 mg/kg in insect whole-body samples, faeces, and tobacco leaves, respectively. NNK was found to be 0.060 mg/kg, 0.035 mg/kg and 0.93 mg/kg in insect whole body samples, faeces and tobacco leaves, respectively. However, NNAL was not detected in both the insect’s whole body and faeces. Recoveries ranged between 95 and 98% for all compounds when spiked at LOD and LOQ. The presence of TSNAs is a biomarker for cancer risk and their presence in insects would point to cancer risk assessment in tobacco feeding insects and any possible TSNA-detoxifying pathways in insects that might prevent mutagenesis caused these compounds.

Insect tea originated from ethnic minority regions in Southwest China: A review on the types, traditional uses, nutrients, chemistry and pharmacological activities.

Insect tea is a special non-Camellia tea produced from the feces of insects in ethnic minority regions in Southwest China. Insect tea was recorded to have the traditional functions to treat summer heat, dampness, digestive disturbance, excessive phlegm, shortness of breath and otitis, etc. AIM OF THIS STUDY: This review provides an updated and comprehensive review of insect tea on the tea types, host plants, tea-producing insects, producing process, traditional uses, nutritional values, chemistry, pharmacological activities, biological safety and toxicity. Moreover, the general challenges and the possible suggestions on insect tea in future were also discussed. The relevant literature on insect tea was obtained from scientific database, including Elsevier, PubMed, Springer, Wiley, Web of Science, Google Scholar, SciFinder, China National Knowledge Infrastructure (CNKI), Baidu Scholar, Wanfang Database, etc. In addition, pertinent information available in Ph.D theses, MSc. dissertations, books, records, and some classical Chinese herbal literature were also included. The references cited in this review were up to September 2022. Insect tea has been traditionally used as popular beverage with various medicinal functions in the ethnic minority regions in Southwest China for centuries. At present, ten types of insect teas are recorded in different regions. Ten species tea-producing insect and fifteen species host plants are used to in the production of the tea. Insect teas were rich in various nutrients including proteins, carbohydrates, fat, minerals, dietary fiber and Vitamins. A total of 71 compounds have been isolated from insect teas, mainly including flavonoids, ellagitannins, chlorogenic acids and other phenolic compounds as well as the alkaloids. Modern studies demonstrated that insect tea had a variety of pharmacological activities in vitro and in vivo, such as anti-diabetic, lipid-lowing and anti-hypertensive, hepato-protective, gastro-intestinal promoting, anti-cancer, anti-mutagenic, antioxidant, and anti-aging activity, etc. Moreover, existing experimental results showed that insect teas are non-toxicity and biological safety. Insect tea is a unique and niche product originated in the ethnic minority regions of Southwest China with various health-promoting benefits. Phenolics including flavonoids, ellagitannins, chlorogenic acids were reported as the main chemical constituents identified from insect tea. Multiple pharmacological activities of insect tea have been reported, which indicated that it had great potential for further development and application as drugs and health-promoting products. However, more studies on the tea-producing insects, host plants, chemistry and pharmacological activity of insect tea, as well as the toxicology should be further investigated.

Faeces tea of cherry caterpillar (larvae of Phalera flavescens) promotes differentiation into myotubes, activates mitochondria, and suppresses the protein expression of ubiquitin ligase in C2C12

Sarcopenia is a syndrome characterised by progressive and systemic loss of skeletal muscle mass and strength. In order to prevent sarcopenia and lead a healthy life, it is necessary to maintain muscle mass and prevent loss of muscle mass. Insect faeces have long been consumed as tea in China, both as a medicine and as a functional food. In the present work, we investigated the efficacy of cherry caterpillar faeces tea (PT) for treating sarcopenia, particularly concerning muscle building and atrophy suppression using C2C12 cells. PT treatment (0.2 mg/mL) increased myotube widths by approximately 40%, and increased the expression levels of Myod, Myog, and MYHC. Additionally, PGC1α, TFAM, SDHA, BCAT, and BCKDH were upregulated in a PT concentration-dependent manner. For PGC1α, which is the transcription coactivator, the protein expression level also increased in a concentration-dependent manner. The findings demonstrated that PT could stimulate PGC1α and activate mitochondria via branched-chain amino acid metabolism and the electron transport chain in C2C12 myoblasts. Furthermore, PT suppressed LPS-induce expression of IL6 and TNFα, and reduced the protein expression levels of the ubiquitin ligases Atrogin-1 and MuRF, which are major cause of muscle atrophy. These results indicated that PT could be effective for muscle building and suppression of atrophy.

中国虫茶的发展现状及其价值研究

Insect tea, a traditional drink for the ethnic minority in southwest China, is a native product of China, and it has a long history. Insect tea is made of the feces of insects that feed on plants and is suggested to has a variety of health protection benefits.In the scientific point of view, insect tea is not tea because it originates from insect feces; however, it is called tea because the way to drink it and the color of the drink are analogous to tea. A tiny amount of insect tea particles in boiled water can make a cup of yellow-red tea without any residues, and its flavor is even better than that of other teas. Thus, it is also called a boutique tea. However, insect tea has not gained sufficient attention, and little basic research and few clinical data are available. At present, due to the household production of insect tea, Insect tea can be produced by a variety of insects and hosting plants，which could cause large difference in composition.Therefore, the in-depth study on the standardized and normalization is necessary and urgent. For a long time, produced through field collection,the low productivity have limited the amount of insect tea for research ，the determination of nutritional components, health care function and biological safety evaluation of main insect tea varieties, the functional development of insect tea, the research and development of insect tea production technology, and the demonstration and application promotion still need further research. Therefore, this paper summarizes the previous research results of insect tea, in order to enhance people's awareness and recognition of insect tea, and play an important theoretical support role for the future development and utilization of insect tea industry.

The Parasite Load of Trypanosoma cruzi Modulates Feeding and Defecation Patterns of the Chagas Disease Vector Triatoma infestans.

Trypanosoma cruzi is the causal agent of Chagas disease, a parasitic zoonosis transmitted mainly through the feces of triatomine insects. Triatoma infestans is the main triatomine vector of this disease in South America. Previous research has shown that T. cruzi infection modifies the behavior of triatomines. We evaluated, for the first time, the effect of parasite load on feeding and defecation behavior, which we quantified by using real-time PCR. The detection time of the host was shorter in infected individuals, and the number of bites increased, while the dejection time was reduced when compared with the non-infected group. A significant correlation between the parasite load and the behavioral changes registered in the infected triatomines was found. These results would indicate that the intensity of T. cruzi infection modulates the feeding and defecation behavior of T. infestans, increasing the vector competence of this triatomine vector.

Epidemiological aspects of Chagas disease in the state of Piauí (Northeast Brazil) in the period 2010–2019

Chagas disease (ChD), caused by the hemoflagellate protozoan Trypanosoma cruzi, is an important morbidity that affects approximately six million people in the American continent. T. cruzi parasites are mainly transmitted to human by the infected feces of blood-sucking triatomine insects. The persistent disease is endemic in many regions of South America, mostly affecting residents of rural areas. The aim of this study was to evaluate epidemiological aspects of ChD in the state of Pi-auí located in northeastern Brazil. This is an analytical cross-sectional study carried out from the collection of data of the Notifiable Diseases Information System (SINAN, in Portuguese, Sistema de Informações de Agravos de Notificação) of suspected and confirmed cases of acute ChD in the state of Piauí, in the period 2010–2019. Associations between T. cruzi positivity and the study variables were determined by the chi-square test or Fisher's exact test and were raised as prevalence ratios (PR) with 95% confidence interval. According to this survey, 517 suspected cases of acute ChD were reported in Piauí, with 70 cases (13.5%) confirmed. In 88.5% of confirmed cases, confirmation occurred by laboratory diagnosis. Most of the confirmed cases occurred in municipalities located in the semiarid region, with the municipality of São João do Piauí presenting the highest number of cases. Regarding sociodemographic data, females represent 55.7% of cases, people over 50 years of age (55.7%), being three cases in people up to 18 years of age, and less than 8 years of schooling (67.1%). 77.9% of confirmed cases had vector transmission as the probable form of infection. The data available in this study conclude that vectorial transmission of ChD in the state of Piauí remains active. This fact is corroborated by the number of notified and confirmed cases of acute ChD, requiring housing improvement programs and more effective epidemiological surveillance to control the transmission of the disease in the state.

Isolation and identification of microbiota from Egyptian common insect pests invading economically important crops

Insects bodies contain multiple distinctive microbial colonies which play key role in the metabolism, development and health of an insect. In the current study bacterial and fungal microbiota were isolated from larval feces and adult wings of two major insect pests in Egypt, the Cotton leaf worm Spodoptera littoralis and the black cutworm Agrotis ipsilon. Isolated pathogens were identified and characterized according to the scientific described methods in such cases. Two different genera of bacteria (Bacillus & Serratia) were isolated from larval feces of both insects while isolated fungus were Paecilomyces variotii and Absidia corymbifera from the feces of A. ipsilon larvae, while Rhozopus stolonifer and Penicillium chrysogenum were isolated from adults wings of the same insect. The fungus Aspergillus flavus, Aspergillus niger and Mucor circinilloides were isolated from feces of S. littoralis larvae. Numerous species of bacteria and fungi had been documented as a bio-agent against many insect pests, and most of them exhibited disruption in life-cycle of different species of Lepidopterous pests. The obtained data sheds light on microbial colonies associated with two major insect pests, however further studies are required to involve the isolated microbiota in the biological control programs of insect pests.

Effect of insect feces (Hermetia illucens) on rice growth and heavy metal migration from polluted soil to rice plant.

Insect feces are a new kind of biological organic fertilizer. Little is known about the influences of insect feces on rice growth and heavy metal migration from soil to rice plant. In this study, the effects of different amounts (CK (0%), T1 (2%), T2 (4%), T3 (6%), and T4 (8%)) of black soldier fly larvae (BSFL) feces on the rice growth and the migration/accumulation of heavy metals (Cd and Pb) were investigated by pot experiments within 2years. The application of insect feces remarkably increased the contents of soil pH, organic matter, ammonium nitrogen, available phosphorus, and potassium. Meanwhile, the insect feces application reduced the weak acid-soluble contents of soil Cd and Pb by 8.3-56.8%, but increased those in the oxidizable (by 22.4-165.7%) and residual (by 1.8-225.6%) states. Except for the T4 treatment in the first year, all fertilization treatments increased the rice yield (up to 43.7% and 195.5% higher than those of CK within 2years). Moreover, the insect feces application reduced the contents of Cd (8.3-66.7%) and Pb (6.4-61.8%) in different parts of rice. Under the same treatment, the metal contents in each part of rice in the second year were lower than those in the first year. The insect feces application decreased the absorption coefficients (24.4-57.5%) and secondary transport coefficients (3.6-44.1%) of Cd and Pb by rice plant. The findings implied that the insect feces might act as effective organic fertilizers for rice plants as well as reducing heavy metal accumulation in rice plants growing in polluted soil.

Effects of insect frass and distiller’s grains on physicochemical properties of saline alkali soil

In this study, a pot experiment was conducted to study the effect of black soldier fly feces and distiller’s grains on the physicochemical properties of moderate saline alkali soil. Results showed that the application of insect feces and distiller’s grains significantly increased the contents of nitrate nitrogen, available phosphorus, available potassium and organic matter in the soil, and reduced soil pH. After the treatment of 2% insect feces mixed with 2% distillates, the available nitrogen content of saline alkali soil was the highest, which was 95.74% higher than that of the control. It can be seen that the combination of 2% insect feces and 2% distiller’s grains was best suited to improve the saline alkali soil properties under the condition of this experiment.

The effect of application of compost and frass from Black Soldier Fly Larvae (Hermetia illucens L.) on growth of Pakchoi (Brassica rapa L.)

Pot experiment was conducted to observe the effect of frass (insect feces) from Black Soldier Fly larvae (BSFL) as soil amendments at different application rates in comparation with other organic fertilizers on Pakchoi (Brassica rapa L). The experimental design was completely random with seven treatments, namely compost, compost+LOB (liquid organic biofertilizer), 5%, 10%, and 15% BSF frass, chemical fertilizer (NPK, equivalent to 10 kg/ha), and soil as a control. After 35 days planting, the result showed that Pakchoi cultivated with compost+LOB, compost, and BSF frass produced the highest biomass compared to control and NPK treatment significantly. The highest total plant weight was achieved in the treatment of Compost+LOB (24.4 gr), followed by 10% BSF frass (23.3 gr), compost (21.8 gr), 15% BSF frass (20.7 gr), 5% BSF frass (19,8 gr), NPK (1.3 gr ) and control soil (0.9 gr). While the chlorophyll content was not significantly different between all treatments, the application of BSF frass tended to be higher but with curly leaf symptoms than other treatments. The population of P solubilizing bacteria and the activity of PME-ase enzyme in soil were also higher in the treatment of compost, compost+LOB, and BSF frass compared to control and NPK treatment.

Changes in speciation, mobility and bioavailability of Cd, Cr and As during the transformation process of pig manure by black soldier fly larvae (Hermetia illucens)

The black soldier fly larvae (BSFL) (Hermetia illucens) are a good candidate for poultry and livestock manure treatment. The harvested insect bodies and feces can be used as animal feed and organic fertilizer. However, heavy metals have a negative impact on the transformation process of the manure by BSFL. Here we evaluated the effects of Cd, Cr and As in pig manure on the growth of BSFL, as well as the mobility and changes in speciation of the metals during the transformation process. The results showed that As significantly reduced the weight of BSFL, but Cr and Cd had no significant effects on BSFL growth. The bioaccumulation factors (BAFs) at various concentrations of Cd (2.8–3.7) were significantly higher than those of Cr (0.44–0.62) and As (0.43–0.45). The heavy metals in pig manure were mainly transferred to BSFL feces, and the distribution percentages of Cd, Cr and As in the BSFL feces were 52.6–62.0%, 90.3–94.2% and 93.0–93.3%, respectively. Cd concentrations in BSFL feces were significantly lower than those in the pig manure, while there were no significant differences in the concentrations of Cr and As between BSFL feces and pig manure (except for the treatment with the addition of 150 mg Cr kg−1). The metal speciation (weak acid soluble, reducible, oxidizable and residual fractions) in BSFL feces obviously changed when compared with pig manure. In BSFL feces, the reducible proportion of Cr decreased while the oxidizable proportion increased. Cd mainly existed in the weak acid soluble and reducible states in pig manure and BSFL feces. For As, the proportions of weak acid soluble and reducible states decreased in BSFL feces. After the BSFL transformation process, the bioavailable fraction of Cr decreased by 17.3–23.1%, but those of Cd and As did not change significantly, except for As in the CK group. These findings contribute to our understanding of the roles of BSFL during the biotransformation process of livestock manure as well as the safe utilization of transformed products.

Multi-heteroatom doped porous carbon derived from insect feces for capacitance-enhanced sodium-ion storage

Abstract The large-scale application of sodium ion batteries (SIBs) is limited by economic and environmental factors. Here, we prepare multi-heteroatom self-doped hierarchical porous carbon (HHPC) with a honeycomb-like structure by one-step carbonization method using high-yield and low-cost biomass silkworm excrement as a precursor. As an anode for SIB, HHPC-1100 exhibits a capacity of 331.7 mA h g−1 at 20 mA g−1, while it also reveals remarkable rate performance and stable long cycle capability due to its abundant pore structure and proper amount of hetero atom doping. Moreover, the synergistic effect of O, N, S, P co-doping in carbon materials on sodium ion adsorption is verified by the first-principles study, which provide a theoretical basis for the prominent electrochemical performance of the material.

Integrating insect frass biofertilisers into sustainable peri-urban agro-food systems

The larvae of black soldier fly (BSF) have shown great promise in transforming organic wastes into a more valuable larval biomass. Importantly, after insects have been harvested the remaining by-product, comprised of the spent substrate and frass (insect faeces), has the potential to be used as a biofertiliser. Three field-scale experiments to investigate whether frass biofertilisers (made from either poultry waste, brewery waste or green market waste) could be successfully incorporated into current small-holder farming practices were undertaken in Ghana, West Africa. In general, BSF frass biofertilisers performed as well as the local practice of amending Zaï planting pits with chicken manure, or incorporating uniformly broadcast fertilisers. For short-cycle cash crops such as chilli pepper and shallots, brewery waste biofertilisers performed better than poultry waste biofertilisers, particularly when added in combination with inorganic NPK fertilisers. For maize, green market waste biofertiliser did not significantly improve yield at applications of either 5 or 10 t/ha, even when combined with inorganic fertilisers. However, frass biofertiliser amendment did significantly reduce the loss of cowpea plants due to Fusarium wilt disease. We hypothesise that the fragments of chitin (originating from 4-5 larval moults) in frass biofertilisers can induce disease resistance in crop plants grown in biofertiliser-amended soil. The benefit of frass as a by-product of insect larvae production can increase the profitability of this burgeoning industry in developing countries, and provide employment opportunities and self-sufficiency in the nutrient supply chain by integrating organic waste management and insect farming into peri-urban agro-food systems.

Modulatory Effect of Trypanosoma cruzi Infective Stages in Different Dendritic Cell Populations in vitro.

Trypanosoma cruzi is a protozoan parasite that infects at least 7 million persons in the world (OMS, 2019). In endemic areas, infection normally occurs by vectorial transmission; however, outside, it normally happens by blood and includes congenital transmission. The persistence of T. cruzi during infection suggests the presence of immune evasion mechanisms and the modulation of the anti-parasite response to a profile incapable of eradicating the parasite. Dendritic cells (DCs) are a heterogeneous population of antigen-presenting cells (APCs) that patrol tissues with a key role in mediating the interface between the innate and adaptive immune response. Previous results from our lab and other groups have demonstrated that T. cruzi modulates the functional properties of DCs, in vitro and in vivo. During vectorial transmission, metacyclic (m) trypomastigotes (Tps) eliminated along with the insect feces reach the mucous membranes or injured skin. When transmission occurs by the hematic route, the parasite stage involved in the infection is the circulating or blood (b) Tp. Here, we studied in vitro the effect of both infective mTp and bTp in two different populations of DCs, bone marrow–derived DCs (BMDCs) and XS106, a cell line derived from epidermal DCs. Results demonstrated that the interaction of both Tps imparts a different effect in the functionality of these two populations of DCs, suggesting that the stage of T. cruzi and DC maturation status could define the immune response from the beginning of the ingress of the parasite, conditioning the course of the infection.

Congenital Chagas Disease in the United States: The Effect of Commercially Priced Benznidazole on Costs and Benefits of Maternal Screening.

.Chagas disease, caused by Trypanosoma cruzi, is transmitted by insect vectors, and through transfusions, transplants, insect feces in food, and mother to child during gestation. An estimated 30% of infected persons will develop lifelong, potentially fatal cardiac or digestive complications. Treatment of infants with benznidazole is highly efficacious in eliminating infection. This work evaluates the costs of maternal screening and infant testing and treatment for Chagas disease in the United States, including the cost of commercially available benznidazole. We compare costs of testing and treatment for mothers and infants with the lifetime societal costs without testing and consequent morbidity and mortality due to lack of treatment or late treatment. We constructed a decision-analytic model, using one tree that shows the combined costs for every possible mother–child pairing. Savings per birth in a targeted screening program are $1,314, and with universal screening, $105 per birth. At current screening costs, universal screening results in $420 million in lifetime savings per birth-year cohort. We found that a congenital Chagas screening program in the United States is cost saving for all rates of congenital transmission greater than 0.001% and all levels of maternal prevalence greater than 0.06% compared with no screening program.

Study on the Adsorption Properties of Different Insect Feces on Cu2+ and Cd2+

In order to understand the adsorption properties of insect feces on heavy metals, White grubs feces and Black soldier fly larvae (BSFL) feces were selected as the adsorption materials to study the adsorption properties of insect feces on Cu2+ and Cd2+. The results showed that the adsorption effect increased significantly with the increasing of the amount of insect feces, and the best adsorption effect were 2g White grubs feces and 4g BSFL feces in 100ml (10mg/L) Cu2+ and Cd2+. With increasing of concentration Cu2+ and Cd2+ (0-20 mg/L), the adsorption quantity of Cu2+ and Cd2+ increased. Adsorption quantity of BSFL feces for Cu2+ was more than White grubs feces, while for Cd2+ was less than White grubs feces when concentration of Cd2+ was over 10mg/g. With the increase of pH, the adsorption rate of White grubs feces and BSFL feces on Cu2+ and Cd2+ decreased significantly.