Epicuticle Research Articles

Assessment of Stress Distribution Around Traditional and Sleeve Fixed Partial Denture Designs: Finite Element Analysis.

The aim of this research is to evaluate/compare the use of traditional versus sleeve fixed partial denture (PD) designs made from different materials on supporting structures. The comparison included three- and four-unit PD cases. Four finite element models are used in the research. The three-unit PD consists of the mandibular second premolar, first molar (as a pontic), and second molar. The four-unit PD includes the first premolar. The PD materials assessed were zirconia, E-max, and Celtra Duo. Bone has been simplified representing it as two cuboids. Each PD has been loaded to two cases over the pontic's central fossa: 300 N compressive, 150 N obliquely applied with 45 degrees forming 24 cases. The three-unit traditional and sleeve PDs material change showed a slight change in cortical bone stress under vertical loading. Under oblique loading, cortical bone Von Mises stresses were higher by about 12 to 15% more than vertical loading. On the other hand, the four-unit PDs showed minor effect by changing PD material, while using sleeve design PD can reduce the cortical bone stresses up to 20% in comparison to traditional PD design. The mucosa and spongy bone were negligibly affected by changing PD material, and the traditional and sleeve designs showed close values to each other. Superiority of sleeve design appeared by reducing cement layer stresses dramatically, while PD body material rigidity affects its response. Within the limitations of this study, the higher rigid PD material can dissipate loadings over it more preferably regarding its effect on the underlying structures. Sleeve PD design is equivalent to the traditional one for three-unit PDs, while it showed better performance with four-unit PDs. Zirconia three-unit PDs' bodies received the lowest stresses and redistributed and transferred the applied load to the underneath structures better than the other two tested materials. This finding was reversed with four-unit PDs.

Chlorbenzuron downregulated HcLCP-17 expression by depressing two 20E-responsive transcription factors Br-C and βFTZ-F1 in Hyphantria cunea (Lepidoptera: Erebidae) larvae.

Cuticular proteins (CPs) play essential roles in forming cuticular structures in insects. However, the specific functions and regulatory mechanisms of CPs remain largely unexplored. In this study, the Larval cuticular protein 17 (HcLCP-17) gene was identified from Hyphantria cunea, a highly destructive and polyphagous forest pest. To investigate the role of HcLCP-17 in cuticular function and transcriptional regulation mediated by 20E-responsive transcription factors (ERTFs), we employed RNA interference (RNAi) and yeast one-hybrid assay techniques. Additionally, we examined the molecular mechanism by which chlorbenzuron, a type of benzoylphenylurea (BPU) that functions as a chitin synthesis inhibitor (CSI), affects the 20E signaling pathway and ultimately regulates HcLCP-17 expression. HcLCP-17 encodes a polypeptide consisting of 393 amino acids, which includes a chitin-binding domain. Silencing HcLCP-17 resulted in a disturbance in the structural organization of the larval cuticle and a notable reduction in chitin levels. HcLCP-17 expression was controlled by the interaction between Broad-Complex (Br-C)and beta Fushi Tarazu Factor-1 (βFTZ-F1) with its promoter fragment. Furthermore, the inhibitory effect of chlorbenzuron on HcLCP-17 expression was found to be potentially mediated by Br-C and βFTZ-F1. The study presents a novel mode of action for the 20E signaling pathway in regulating the expression of CPs and reveals the potential mode-of-action of BPUs in insect cuticles. These findings provide a theoretical basis for future utilization of LCP-17 as a pesticide target making a significant contribution to the development of effective pest management strategies. © 2024 Society of Chemical Industry.

On the hygrothermal environment of spaces with exposed walls – thermal bridging effect of mortar layers of cement and lime

On the hygrothermal environment of spaces with exposed walls – thermal bridging effect of mortar layers of cement and lime

Characterization of an RR-2 cuticle protein DcCP8 and its potential application based on SPc nanoparticle-wrapped dsRNA in Diaphorina citri.

The insect cuticle consists of chitin fibers and a protein matrix, which plays an important role in protecting the body from invasion of various pathogens and prevents water loss. Periodic synthesis and degradation of the cuticle is required for the growth and development of insects. Key genes involved in cuticle formation have long been considered a potential target for pest control. In this study, a member of the RR-2 subfamily of cuticular protein 8 (DcCP8) was identified from the Diaphorina citri genome database. Immunofluorescence analysis suggested that DcCP8 was mainly located in the Diaphorina citri exocuticle and can be induced to up-regulate 12 h following 20-hydroxyecdysone (20E) treatment. Silencing of DcCP8 by RNA interference (RNAi) significantly disrupted the metamorphosis to the adult stage, and improved the permeability of the cuticle. Transmission electron microscopy (TEM) analysis revealed that the synthesis of the exocuticle was impressed after silencing of DcCP8. Furthermore, the recombinant DcCP8 protein exhibited chitin-binding properties in vitro, down-regulation of DcCP8 significantly inhibited expression levels of chitin metabolism-related genes. Additionally, a sprayable RNAi method targeting DcCP8 based on star polycation (SPc) nanoparticles-wrapped double-stranded RNA (dsRNA) significantly increased Diaphorina citri mortality. Transcriptome sequencing further confirmed that genes associated with the endocytic pathway and immune response were up-regulated in Diaphorina citri after SPc treatment. The current study indicated that DcCP8 is critical for the formation of Diaphorina citri exocuticles, and lays a foundation for Diaphorina citri control based on large-scale dsRNA nanoparticles. © 2024 Society of Chemical Industry.

Effects of Larch Woolly Adelgid Infestation on Morphological, Histological and Allelochemical Traits of European Larch Needles

The study was carried out to assess the effect of the larch wooly adelgid Adelges laricis Vallot (Hemiptera: Adelgidae) infestation on its secondary host, the European larch Larix decidua Mill. Morphology and anatomy of adelgid-infested needles, and content of defense phenolic compounds including individual flavonoids isorhamnetin, kaempferol, quercetin, rutin, catechin, epicatechin, apigenin, ampelopsin and taxifolin, were analyzed. The amount of total phenols in needles from adelgid-infested twigs of L. decidua increased following the development of the A. laricis population, from the end of April until the end of June. The most abundant among flavonoids were the flavanols, mainly catechin and epicatechin, which predominated in the larch needles during the whole period of adelgid infestation. The content of catechin and epicatechin increased following the increase in the adelgid population number. An increase in content occurred also in flavanonols ampelopsin and taxifolin, while the content of the flavonol kaempferol decreased as the population number of A. laricis increased. The analysis of the anatomical structure of needles showed changes in the shape of the needles, the presence of a thicker layer of epicuticular waxes, and a higher number of mesophyll layers as a result of adelgid feeding.

CRISPR-Cas9-mediated enhancement of Beauveria bassiana virulence with overproduction of oosporein

Biocontrol agents play a pivotal role in managing pests and contribute to sustainable agriculture. Recent advancements in genetic engineering can facilitate the development of entomopathogenic fungi with desired traits to enhance biocontrol efficacy. In this study, a CRISPR-Cas9 ribonucleoprotein system was utilized to genetically improve the virulence of Beauveria bassiana, a broad-spectrum insect pathogen used in biocontrol of arthropod pests worldwide. CRISPR-Cas9-based disruption of the transcription factor-encoding gene Bbsmr1 led to derepression of the oosporein biosynthetic gene cluster resulting in overproduction of the red-pigmented dibenzoquinone oosporein involved in host immune evasion, thus increasing fungal virulence. Mutants defective for Bbsmr1 displayed a remarkable enhanced insecticidal activity by reducing lethal times and concentrations, while concomitantly presenting negligible or minor pleiotropic effects. In addition, these mutants displayed faster germination on the insect cuticle which correlated with higher density of free-floating blastospores in the hemolymph and accelerated mortality of the host. These findings emphasize the utility of genetic engineering in developing enhanced fungal biocontrol agents with customized phenotypic traits, and provide an efficient and versatile genetic transformation tool for application in other beneficial entomopathogenic fungi.

Efficient Homogenization of Multicomponent Metamaterials: Chiral Effects

Herein, an efficient homogenization procedure is extended based on a Haydock representation of the microscopic wave operator for the calculation of the retarded macroscopic dielectric response of a binary periodic composite to the case of an arbitrary number of components of arbitrary composition. As a test, this numerical procedure is applied to the calculation of the optical properties of a Bouligand structure, made up of a large number of anisotropic layers stacked on top of each other and progressively rotated. This system constitutes a photonic crystal with circularly polarized electromagnetic normal modes, naturally occurring in the cuticle of several arthropods. It presents a gap for one helicity, which corresponds to the observation of circularly polarized strong metallic like reflections. This numerical procedure is validated through its good agreement with the results of alternative formulations, including an analytical solution for this simple chiral system.

A histological procedure for the study of insect chitinized tissues in light microscopy

We present a procedure to obtain histological preparations of insect cuticles for their observation in ligth microscopy. The use of EDTA (ethylendiaminetetraacetic acid), as a chelator of certain metals present in chitin, soften the tissues and allows us to cut them. The tissues were embbeding in plastic resines in two different protocols and cutted by cryostat (20–30 μm) and ultramicrotome (4 μm) respectively. This procedure, based on traditional histology products, allows us to obtain preparations observable under an optical microscope, facilitating the study of the internal structure of the cuticule, e.g. visualizing the different layers or structures such as glands or setae.

Neuropeptide Bursicon and its receptor-mediated the transition from summer-form to winter-form of Cacopsylla chinensis.

Seasonal polyphenism enables organisms to adapt to environmental challenges by increasing phenotypic diversity. Cacopsylla chinensis exhibits remarkable seasonal polyphenism, specifically in the form of summer-form and winter-form, which have distinct morphological phenotypes. Previous research has shown that low temperature and the temperature receptor CcTRPM regulate the transition from summer-form to winter-form in C. chinensis by impacting cuticle content and thickness. However, the underling neuroendocrine regulatory mechanism remains largely unknown. Bursicon, also known as the tanning hormone, is responsible for the hardening and darkening of the insect cuticle. In this study, we report for the first time on the novel function of Bursicon and its receptor in the transition from summer-form to winter-form in C. chinensis. Firstly, we identified CcBurs-α and CcBurs-β as two typical subunits of Bursicon in C. chinensis, which were regulated by low temperature (10 °C) and CcTRPM. Subsequently, CcBurs-α and CcBurs-β formed a heterodimer that mediated the transition from summer-form to winter-form by influencing the cuticle chitin contents and cuticle thickness. Furthermore, we demonstrated that CcBurs-R acts as the Bursicon receptor and plays a critical role in the up-stream signaling of the chitin biosynthesis pathway, regulating the transition from summer-form to winter-form. Finally, we discovered that miR-6012 directly targets CcBurs-R, contributing to the regulation of Bursicon signaling in the seasonal polyphenism of C. chinensis. In summary, these findings reveal the novel function of the neuroendocrine regulatory mechanism underlying seasonal polyphenism and provide critical insights into the insect Bursicon and its receptor.

Neuropeptide Bursicon and its receptor-mediated the transition from summer-form to winter-form of Cacopsylla chinensis

Seasonal polyphenism enables organisms to adapt to environmental challenges by increasing phenotypic diversity. Cacopsylla chinensis exhibits remarkable seasonal polyphenism, specifically in the form of summer-form and winter-form, which have distinct morphological phenotypes. Previous research has shown that low temperature and the temperature receptor CcTRPM regulate the transition from summer-form to winter-form in C. chinensis by impacting cuticle content and thickness. However, the underling neuroendocrine regulatory mechanism remains largely unknown. Bursicon, also known as the tanning hormone, is responsible for the hardening and darkening of the insect cuticle. In this study, we report for the first time on the novel function of Bursicon and its receptor in the transition from summer-form to winter-form in C. chinensis. Firstly, we identified CcBurs-α and CcBurs-β as two typical subunits of Bursicon in C. chinensis, which were regulated by low temperature (10 °C) and CcTRPM. Subsequently, CcBurs-α and CcBurs-β formed a heterodimer that mediated the transition from summer-form to winter-form by influencing the cuticle chitin contents and cuticle thickness. Furthermore, we demonstrated that CcBurs-R acts as the Bursicon receptor and plays a critical role in the up-stream signaling of the chitin biosynthesis pathway, regulating the transition from summer-form to winter-form. Finally, we discovered that miR-6012 directly targets CcBurs-R, contributing to the regulation of Bursicon signaling in the seasonal polyphenism of C. chinensis. In summary, these findings reveal the novel function of the neuroendocrine regulatory mechanism underlying seasonal polyphenism and provide critical insights into the insect Bursicon and its receptor.

Mandible composition and properties in two selected praying mantises (Insecta, Mantodea).

Insects process their food with their cuticle-based mouthparts. These feeding structures reflect their diversity and can, in some cases, showcase adaptations in material composition, mechanical properties, and shape to suit their specific dietary preferences. To pave the way to deeply understand the interaction between mouthparts and food and to determine potential adaptations of the structures to the food, this study focuses on the mandibles of two praying mantis species. Gongylus gongylodes feeds mainly on Diptera, and Sphodromantis lineola forages on larger prey. Employing scanning electron microscopy, the mandibular morphologies were analyzed. The degree of the cuticle tanning was tested using confocal laser scanning microscopy. Furthermore, the contents of transition and alkaline earth metals in the mandible cuticle were studied using energy-dispersive X-ray spectroscopy and the mechanical properties tested by nanoindentation. We found that S. lineola mandibles show pronounced gradients of Young's modulus and hardness from the basis to the tip, which might be an adaptation against high stresses during biting and chewing. G. gongylodes, in contrast, did not show pronounced gradients, which may indicate that there is less stress involved in feeding-necessary to test in future studies. The mechanical properties of manidibles in both species are related to the degree of cuticle tanning but also positively correlate with the content of magnesium. These findings enrich our understanding of insect cuticle biology but also present new sets of data on praying mantis structures.

Evaluation of polyaryletherketone materials as post-core abutments for removable partial dentures: A finite element analysis

ObjectiveThe purpose was to compare the biomechanical behavior of single-piece post-core restorations made from polyaryletherketone materials with fiber post-core restorations when serving as abutments for RPD using finite element analysis (FEA). MethodsPhantom maxillary central incisor and mandibular second premolar were trimmed 1-mm coronally to cemento-enamel junction; root canals were enlarged and the teeth were scanned. Data was transferred to a solid modeling software.Twenty four models, including six post-core restorations:glass-fiber post/composite core (GFH/GFL) and single-piece post-core groups as, PEKK(PKH/PKL);Ti02-reinforced PEEK(TH/TL);ceramic reinforced PEEK(CeH/CeL);carbon fiber reinforced PEEK(CaH/CaL);glass fiber reinforced PEEK(GFPH/GFPL) with hybrid ceramic/lithium disilicate crowns on each tooth were constructed.Loads of 100 N for central incisor, and 300 N for premolar in a 45°oblique direction were applied to simulate masticatory forces. Clasp removal force of a RPD was simulated as 5 N vertically.FEA was employed to evaluate the von Mises stresses.Strain at cement layer was also investigated. ResultsCaH/CaL groups revealed the lowest stress for both teeth at root while TH/TL groups revealed the highest stress. The lowest stress values in the post-core were in GFH/GFL groups while the highest stress occurred in the CaH/CaL groups for both teeth. SignificanceGlass-fiber post-cores exhibited the lowest stresses in the post under masticatory and clasp removal forces. It may suggest a potentially lower risk of post fracture compared to polyaryletherketone group materials. TiO2-reinforced PEEK post-cores exhibited the lowest stresses among PAEK materials, indicating a potentially high fracture resistance.

Experimental Research of Heating Characteristics on An Air Source Heat Pump System with Capillary Direct Floor Radiant

A new-type air source heat pump system with capillary direct floor radiant (ASHPCFR) was proposed, in which the vapor refrigerant condensed directly in the capillary under the floor to heat the room. Theoretical calculation of heat transfer in the capillary floor was conducted. The results indicated that the optimal capillary spacing should be 100 mm and it was best for a thickness smaller than 60 mm with the floor cement layer. An experimental apparatus for the ASHPCFR system was developed, and its low-temperature heating process and normal heating process were experimentally investigated. Experimental results showed that when the outdoor temperature was -5 °C, the indoor air temperature reached 20 °C after 120 minutes of running, while the temperature difference of a 20 mm thick cement floor could reach 1.73 °C and its maximum temperature could reach 27.5 °C. When the outdoor temperature was -10 °C, the system heating COP was stable at 3.06.

Effect of loading angle and fabrication materials on stress distribution with periodontal splint in compromised periodontal tissues: a finite element study

Objective: To evaluate the effect of polyetheretherketone (PEEK) periodontal splints and splints made from other materials under static loading on stress distributions in periodontal tissues, cement layer, and splints themselves. Methods: A finite element model based on cone-beam CT imaging data of a 25-year-old male patient (treated at the Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University in October 2021 for a cracked maxillary molar) with a healthy and intact mandibular dentition and periodontal health was constructed. The finite element model included anterior mandible dentition, mandibular bone model without bone resorption (WBR group), a periodontally compromised mandible model (control group), and three types of periodontal splints: a PEEK periodontal splint (0.7 mm thick, Young's modulus: 4.1 MPa), a fiber-reinforced resin (FRC) splint (1.0 mm thick, Young's modulus: 37.0 MPa), and a titanium splint (1.2 mm thick, Young's modulus: 110.0 MPa). The bone resorption models fixed with different periodontal splints constituted the experimental groups (PEEK group, FRC group and titanium group). Loading of 100 N was applied on the midpoint of the incisal edge of tooth 41. The direction was set at 0°, which was parallel to the long axis of the tooth and downward. The buccal to lingual and downward angles were 30°and 60°, respectively, perpendicular to the long axis of the tooth and 90° to the lingual side. The finite element analysis software was utilized to analyze the stress distribution characteristics of the periodontal tissues, adhesive layer, and the splint itself in the anterior mandibular teeth among the different group. Results: Under the different loading simulation, in the control group, the maximal von Mises stresses of periodontal ligament and bone were 15.7-50.2 MPa and 38.8-130.3 MPa, respectively, and in the WBR group, the maximal von Mises stresses of periodontal ligament and bone were 3.6-6.4 MPa and 16.5-42.7 MPa, respectively. Under the same loading conditions, the magnitude of maximal von Mises stresses in periodontal tissues in the PEEK group was 4.6-6.2 MPa, and the magnitude of stresses in the periodontal ligament of 41 teeth in the WBR group was similar to that in the PEEK group, but higher than that in the FRC and titanium groups. The maximal von Mises stresses of each group is primarily distributed in the periodontal ligament and alveolar bone at the cervical area of the tooth. The higher the elastic modulus of the splint, the higher its own maximal von Mises stresses, and the smaller the maximal principal stresses transmitted to the adhesive layer. In the PEEK group and titanium group, the stress distribution area in the adhesive layer and the splint was near the splint connection adjacent to tooth 41. Conclusions: Periodontal splints fabricated from three types of materials, are effective in distributing stress within the periodontal tissues of the abutment teeth. Compared to FRC and titanium group, the higher PEEK splint stress value was obtained, and the smaller the stress value was transmitted to its adhesive layer.

Role of chemistry in modern world: Boons and banes of chemical coating effects on human health

Water is the principal component of fresh fruits and Vegetable which constitutes between 80 to 90% of a produce's fresh weight. Fruits are covered by a layer of natural wax which acts as a barrier to reduce moisture loss and at the same times give the fruit a shiny surface. Besides orange shellac, other common materials used for these purposes include starch, carrageenan, alginates, wood rosin and various waxes. Panko breadcrumbs are crucial to the vegetables being very crispy. The coatings are typically made from natural materials, such as CHI, cellulose, and pectin that are safe for human consumption. The multilayer coating method has been shown to be effective in extending the shelf life of a variety of fresh fruits and vegetables, including apples, strawberries, tomatoes, and cucumbers. Both natural waxes (carnauba, shellac, beeswax or resin) and petroleum-based waxes (usually proprietary formulae) are used, and often more than one wax is combined to create the desired properties for the fruit or vegetable being treated. However, chemical treatments, such as copper sulfate, rhodamine oxide, malachite green, and deadly carbide, used on green vegetables to enhance coloration and freshness, are often counterproductive to their nutritional value. There is multiple health risks associated with these chemicals. This study reveals that food preservatives have made a big difference in eating food every day. They help keep the food safe because they add these chemicals to prevent spoilage and retard prevent or control undesirable changes in flavor, color, texture, or consistency of food and nutritive value of food which means the Control natural spoilage of food.

Mechanism of Exogenous Jasmonic Acid-Induced Resistance to Thrips palmi in Hemerocallis citrina Baroni Revealed by Combined Physiological, Biochemical and Transcriptomic Analyses

Jasmonic acid (JA) is a regulator of plant resistance to phytophagous insects, and exogenous JA treatment induces plant insect resistance. This study investigated the mechanism of exogenous JA-induced resistance of Hemerocallis citrina Baroni (daylily) to Thrips palmi at the biochemical and molecular levels. Daylily leaves sprayed with JA showed significantly higher levels of secondary metabolites—tannins, flavonoids, and total phenols, and activity of defense enzymes—peroxidase, phenylalanine ammonia lyase, polyphenol oxidase, and protease inhibitor (PI) than control leaves; the most significant effects were observed with 1 mmol L−1 JA. Owing to an improved defense system, significantly fewer T. palmi were present on the JA-treated plants than control plants. The JA-treated leaves had a smoother wax layer and fewer stomata, which was unfavorable for insect egg attachment. The differentially expressed genes (DEGs) were significantly enriched in insect resistance pathways such as lignin and wax biosynthesis, cell wall thickening, antioxidant enzyme synthesis, PI synthesis, secondary metabolite synthesis, and defense hormone signaling. A total of 466 DEGs were predicted to be transcription factors, mainly bHLH and WRKY family members. Weighted gene co-expression network analysis identified 13 key genes; TRINITY_DN16412_c0_g1 and TRINITY_DN6953_c0_g1 are associated with stomatal regulation and lipid barrier polymer synthesis, TRINITY_DN7582_c0_g1 and TRINITY_DN11770_c0_g1 regulate alkaloid synthesis, and TRINITY_DN7597_c1_g3 and TRINITY_DN1899_c0_g1 regulate salicylic acid and ethylene biosynthesis. These results indicate that JA treatment of daylily improved its resistance to T. palmi. These findings provide a scientific basis for the utilization of JA as an antagonist to control T. palmi in daylily.

Elucidating structure and metabolism of insect biomaterials by solid-state NMR

Among the many natural biomaterials for which information on atomic-level structure and reorientational motion can offer essential clues to function, insoluble multi-component composites with limited degrees of order are among the most challenging to study. Despite its limited sensitivity, solid-state NMR (ssNMR) is often the technique of choice to ferret out these details in carbon- and nitrogen-rich materials: this spectroscopic approach can probe many biomaterials in their native or near-native states, either with or without the introduction of stable NMR-active isotopes, or with the assistance of dynamic nuclear polarization technology. During a span of close to four decades, such research targets and ssNMR approaches have been exemplified by insects, a diverse and evolutionarily agile group of organisms with global impacts that include ecology, agriculture, and human disease. In this short review, we present case studies on insect cuticles that range from protective exoskeletons and egg capsules to the wing structures that enable flight and showcase nature's awe-inspiring beauty, highlighting the use of ssNMR spectroscopy to profile chemical composition, elucidate macromolecular architecture, and monitor metabolic development in these fascinating biological assemblies.

Classification, biology and entomopathogenic fungi-based management and their mode of action against Drosophila species (Diptera: Drosophilidae): a review.

This review provides a comprehensive analysis of the classification, biology, and management of Drosophila species (Diptera: Drosophilidae) with a focus on entomopathogenic fungi (EPF) as a biocontrol strategy. Drosophila species, particularly Drosophila suzukii, and Drosophila melanogaster have emerged as significant pests in various agricultural systems, causing extensive damage to fruit crops. Understanding their taxonomic classification and biological traits is crucial for developing effective management strategies. This review delves into the life cycle, behavior, and ecological interactions of Drosophila species, highlighting the challenges posed by their rapid reproduction and adaptability. The review further explores the potential of EPF as an eco-friendly alternative to chemical pesticides. The mode of action of EPF against Drosophila species is examined, including spore adhesion, germination, and penetration of the insect cuticle, leading to host death. Factors influencing the efficacy of EPF, such as environmental conditions, fungal virulence, and host specificity, are discussed in detail. By synthesizing current research, this review aims to provide valuable insights into the application of EPF and to identify future research directions for enhancing the effectiveness of EPF-based control measures against Drosophila species.

Analysis of Implication of Phase Change Material Enclosed Design on Thermal Performance of Solar Collector

Abstract The energy, which is converted from the sun rays, was called solar energy. This is very essential renewable energy converting methods that had a higher influence for water distilling system. Here, the implication of the Phase Change Materials (PCM) in that exit was analyzed. This energy from the sun is trapped in paraffin wax for the storing system whiles the greater radioactive effect. This trapped heat power than utilized for increase the fluid temperature during the lesser radioactive times. Here, the Implication of the heat storing units on the exit temperature was analyzed. Furthermore, Thermal solar collector was analyzed for the following years 2021 and 2022. The Concentric PC is placed over the top of the collector of the solar rays. And then, a paraffin wax layer was placed under the solar absorber plate like the back-up to store the LH Energy. The uniform rate of flow was produced a greater exit temperature of water the two years. The efficiency rate increased 11–14% and the collected energy gain rate enhanced 18–19%. The peak water temperature was 61°C & 60°C for the 2 consecutive years for Paraffin wax integrated solar collecting unit. At the same time the efficiency of heat energy is peak at 49% in January 2022. The solar collector efficiency primarily relies on the design and construction of collector itself.

Influence of nanopesticide surface chemistry on adsorption to plant cuticle and wax layer: The role of zeta potential and wetting

Nanopesticidal adsorption on plant surfaces is a critical determinant of their application efficacy, persistence, and ecological impact. In this study, we systematically investigate the impact of surface chemistry on the attachment of nanopesticides for seven different nanocarriers: ethyl lauroyl arginate (ELA, cationic), cocamidopropyl betaine (CAPB, zwitterionic), tween 80 (TW80, nonionic), β-cyclodextrin (βCD), sodium dodecyl sulfate (SDS, anionic), whey protein isolate (WPI), and poloxamer 407 (PXL, nonionic). Azadirachtin from neem seed extract was employed as a model pesticide active ingredient. The nanopesticides were characterized using dynamic light scattering, UV–visible spectroscopy, static contact angle (SCA, θ), and zeta (ζ) potential measurements. Pepper leaves (ζ = −11.6 mV) and candelilla wax (ζ = −2.6 mV) films were utilized to analyze the effect of nanocarrier chemical composition on nanopesticide adsorption. Fluorescence microscopy was utilized to quantify the adsorption of nanopesticides (with fluorophore tagging) on the substrates. It was found that the choice of nanocarrier significantly influenced the adsorption behavior. Nanopesticides with ELA corona, which was cationic with a zeta potential of ∼+19 mV and θ of ∼ 61°, exhibited the highest affinity towards the leaf cuticle and wax substrates, attributed to favorable electrostatic interactions forces. Conversely, nanopesticides with SDS (ζ = −48 mV; θ = 45°), WPI (ζ = −24 mV; θ = 54°), and PXL (ζ = −31 mV; θ = 64°) corona demonstrated the least adsorption. These findings indicate a weak correlation between the wetting behavior of nanopesticide suspensions and nanopesticide adsorption on plant and wax surfaces, as well as a strong correlation between nanopesticide zeta potential and nanopesticide adsorption. These findings heuristically recommend that aqueous cationic and zwitterionic nanocarriers for pesticides provide superior adsorption characteristics on pepper leaves and candelilla wax surfaces. Aqueous macromolecular carriers such as PXL and WPI have performed less effectively in adherence compared to shorter chain amphiphiles with similar zeta potential and wetting characteristics, indicating that the steric and osmotic chain effects of hydrophilic macromolecules hinder the adsorption relative to shorter chains.