Whereas many aquatic species experience daily temperature changes, it is unclear whether temperature fluctuations can affect the accumulation of trace metal independently from changes in mean temperature. Here, the impact of oscillating temperature (MTosc: 7/23 °C, 24 h/cycle) on the accumulation of both inorganic Hg (IHg) and monomethylmercury (MeHg) in Daphnia magna was investigated, by comparing it to a constant temperature treatment with the same average temperature (MTconst: 15 °C). The effects of mean temperature were tested by comparing this latter to a similar treatment with higher temperature (HTconst: 23 °C). Trophic pathway accumulation was estimated by using Chlorella vulgaris labeled with stable Hg isotopes, whereas the water pathway accumulation was estimated by directly spiking Hg isotope solutions into the D. magna incubators. The concentrations of IHg decreased in D. magna under oscillating temperature conditions for both trophic and water pathways by at least 42 % and 25 %, respectively, compared to the MTconst treatment. In contrast, the accumulation of MeHg was reduced only in the trophic pathway in these conditions, whereas it was enhanced in the water pathway. Patterns similar to IHg were observed for Fe, Zn, and Cu in the trophic pathway, but only for Cu in the water pathway. In contrast, no difference on the accumulation of rare earth elements was observed among temperature treatments. These results shed a new light on trace metal accumulation under near-realistic temperature conditions.