Innate Potential Research Articles

Blue Butterfly

The "butterfly" is a symbol of the mind, soul, and transformation (Bradway & McCoard, 1997, p. 157). Jung believed that just as an acorn becomes an oak tree and a larva a butterfly, the role of the psycho-clinician is to discover and fulfill the client's innate and profound potential (The Charleston Jung Society, 2020). This absolute trust in human beings and their transformative potential is the guiding principle that Jungian psychologist should have. I (the author) must say that Jung's philosophy is especially poignant to me, as I have has such a tumultuous like that I think it is rare for someone to have achieved such great growth as I have. The butterfly transforms from egg to larva, larva to chrysalis, and chrysalis to butterfly, abandoning its old self and transforming into a new self in stages. Even though I am not as beautiful in body and mind as a butterfly, I feel a sense of familiarity with a butterfly and take my hat off to show my respect for its power of 'rebirth.' As I have walked the path of individuation as a Jungian-oriented psychologist, the butterfly has always been with me and guided me along my psychological journey. As a human being and a Jungian-oriented psychologist, the symbol of the butterfly has taught me about rebirth, healing, and synchronicity.

Thermodynamic Assessment of Sacrificial Oxidant Potential, H2O/O2 Potential, and Rate-Overpotential Relationship to Examine Catalytic Water Oxidation in Nonaqueous Solvents.

The water oxidation reaction (WOR), which is pivotal to storing energy in chemical bonds, requires a catalyst to overcome its inherent kinetic barrier. In bulk solutions, sacrificial oxidants (SOs) can regenerate the catalysts to ensure that the homogeneous WOR can be operated with long-term consistent performance. To implement this strategy for organic WOR systems, we modified four common SOs with tetra-n-butylammonium ([NBu4]+)─[NBu4]2[Ce(NO3)6], [NBu4][IO4], [NBu4][HSO5], and [NBu4]2[S2O8]─and examined their chemical stability and electrochemical behaviors in various organic solvents. We also derived the organic-solvent-associated redox potential of H2O/O2 in organic media (EH2O/O2(org)) using open-circuit potential measurements of the H+/H2 redox couple and the related thermochemical cycle. Our findings indicate that the EH2O/O2(org) varies with solvent identity and can be adjusted by changing the [H2O], [acid], and [base] levels; thus, the SO should be carefully selected for WOR, because the innate redox potentials of SOs are not always higher than EH2O/O2(org) under the studied conditions. Lastly, we obtained catalyst-performance-related insights via a rate-overpotential free-energy relationship by calculating the overpotentials of previously studied WOR systems in organic media.

Meningkatkan Hasil Belajar Pendidikan Agama Katolik dengan Model Problem Based Learning (PBL) Fase C Kelas V SDN 105302 Tangkahan

Education is simply defined as human efforts to nurture and develop innate potential both physically and spiritually in accordance with the values present in their society and culture. The Merdeka Curriculum is a curriculum with diverse intracurricular learning and improved content, allowing students sufficient time to learn concepts and strengthen skills. The purpose of Catholic Religious Education in schools is to encourage students to interact or communicate in understanding, confronting, and living their faith. However, at SDN 105302 Tangkahan, there are challenges in teaching Catholic Religious Education, namely the low learning outcomes of students due to more conventional and non-interactive teaching methods. There is a lack of use of engaging learning media and technology that is considered irrelevant to students' daily lives. This research aims to improve student learning outcomes in Catholic Religious Education through the application of the Problem Based Learning (PBL) model. This study uses a classroom action research method with fifth-grade students in phase C at SDN 105302 Tangkahan as research subjects. Data was collected through observation, tests, and interviews. The results show a significant improvement in concept understanding, collaboration skills, and student learning outcomes after the implementation of the PBL model in Catholic Religious Education.

Therapeutic and Diagnostic Implications of Carbon Dot: An Advancement in the Avenue towards Cancer, Diabetes and Neurodegenerative Disorders.

Carbon dots (CDs), by virtue of their electrical and optical properties, emit intense light and fluorescence. They have attributes like photostability, high quantum yield (QY), high emission, and scalability. In the recent past, theranostic-CDs have been widely used in sensing, imaging, and medication administration. Furthermore, CDs may provide significant promise to detect and ability to cross blood-brain barrier (BBB) along with a drug to treat numerous neurodegenerative disorders (ND), such as Parkinson's disease (PD), Alzheimer's disease (AD), and multiple sclerosis. This review aims at exploring the immense utility of CDs in the arena of theranostics. The immense utility of CDs was investigated through a systematic exploration of previously published data in the relevant field. The application of CDs is well extended to treat life-threatening disorders, like diabetes and cancer. In order to keep harmful substances out of the brain, the blood- -brain barrier (BBB) forms a defensive barrier. Water and gases being simple molecules can traverse the BBB without getting filtered out, but several large molecules suffer to reach the site of action owing to their poor solubility. CDs have recently been employed for delivering drugs to the brain and to assess the central nervous system. An in-depth study of relevant literature indicates that CDs are the next-generation carrier with an innate potential to subside the drawbacks of conventional nanoparticles. This review illustrates several biomedical applications of CDs, primarily focusing on neurological illnesses, diabetes, and cancers. It also confers the usefulness of CDs in diagnostic imaging.

SAHA potentiates the activity of repurposed drug promethazine loaded PLGA nanoparticles in triple-negative breast cancer cells.

Triple-negative breast cancer (TNBC) is considered the most aggressive form of breast cancer owing to the negative expression of targetable bioreceptors. Epithelial to mesenchymal transition (EMT) associated with metastatic abilities is its critical feature. As an attempt to target TNBC, nanotechnology was utilised to augment the effects of drug repurposing. Concerning that, a combination therapeutic module was structured with one of the aspects being a repurposed antihistamine, promethazine hydrochloride loaded PLGA nanoparticles. The as-synthesized nanoparticles were 217 nm in size and fluoresced at 522 nm, rendering them suitable for theranostic applications too. The second feature of the module was a common histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), used as a form of pre-treatment. Experimental studies demonstrated efficient cellular internalisation and significant innate anti-proliferative potential. The use of SAHA sensitised the cells to the drug loaded nanoparticle treatment. Mechanistic studies showed increase in ROS generation, mitochondrial dysfunction followed by apoptosis. Investigations into protein expression also revealed reduction of mesenchymal proteins like vimentin by 1.90 fold; while increase in epithelial marker like E-Cadherin by 1.42 fold, thus indicating an altered EMT dynamics. Further findings also provided better insight into the benefits of SAHA potentiated targeting of tumor spheroids that mimic solid tumors of TNBC. Thus, this study paves the avenue to a more rational translational validation of combining nanotherapeutics with drug repurposing.

Pengaruh Fasilitas Sekolah Terhadap Motivasi Belajar Siswa Kelas 5 SDN 1 Beluk Tahun Pelajaran 2023/2024

This research aims to determine the effect of school facilities on student learning motivation SDN 1 Beluk. This study discusses the effect of school facility to students` learning ability and motivation. Student creativity can be obtained in the learning process through various interactions and learning experiences and is based on the individual`s innate potential an environmental influences. The method in this research is ex post facto with a quantitative approach. The population in this study was class 5 of SDN 1 Beluk, totaling 99 students, however, due to limited funds, time and energy, in this study a sample of 20 students was used which was determined using the Proportional Random Sampling formula. The data collection technique uses a questionnaire regarding school facilities and student learning motivation. Research data analysis uses simple linear regression. The research results obtained have an impact on student learning facilities 43.0% school facilities are the most important factor due to the large.

The development of the Moments of Flourishing Experience Scale: a new scale to measure positive, affect-based flourishing state experiences

ABSTRACT Flourishing has been a focus of positive psychology, with increased attention to its translation into psychotherapy practice. Flourishing is an emergent process manifested in momentary experiential states characterized by positive emotions. A conceptualization of flourishing as an innate potential that psychotherapeutic interventions can help cultivate informs the development of this scale. The current study details the development of the Moments of Flourishing Experience Scale (MFES), a 23-item scale. The data were obtained from four different studies involving a total of 1472 adults (normative sample n = 1312; clinical sample n = 160). A bifactor model with four distinct positive affective experience subscales, yielded by factor analysis and then supported by a confirmatory factor analysis, was as follows: Calm & Self-Acceptance; Vitality; Generosity of Spirit & Gratitude; and Connection & Purpose. Across studies, the MFES showed discriminant validity from psychopathology measures and convergent validity with positive psychological functioning measures. The overall scale, as well as each of four subscales, also demonstrated strong reliability in both general and clinical populations. The MFES has initial psychometric support for the study of flourishing and shows promise as a tool to study flourishing as both outcome and process in psychotherapy.

Fulfillment of Basic Psychological Needs Through Eudaimonic Activities on Subjective Well-Being of Islamic Elementary School Children

This research further examines and analyzes the fulfillment of the basic psychological needs of subjective wellbeing of Madrasah Ibtidaiyah children through Eudaimonic Activities. The mixed methods design used in this research provides a strong framework for capturing the diverse nature of children's experiences with eudaimonic activities. This research was carried out at Jatinagor Elementary School. The population is fifth-grade students at Jatinagor Elementary School. The sampling technique used in data collection is convenience sampling. Participants in this study were pre-adolescent students aged 11 to 12 years. The number of participants in this study was 81 children. Data analysis was carried out by calculating the overall score obtained from the BPNFS and SPANE measuring instruments and then averaging them. The interesting finding was that playing online games was a common form of entertainment among the participants. This suggests that digital play can be an important aspect of children's lives, potentially contributing to their eudaimonic wellbeing if managed appropriately. These insights pave the way for further research into how online games can be integrated into educational environments in ways that support children's developmental needs. Additionally, the results of this study provide concrete examples of eudaimonic activities, which can be invaluable for educators and parents who wish to understand and develop children's innate potential.

Periodontal granulation tissue - To remove or not to remove, that is the question.

Formation of granulation tissue is a fundamental phase in periodontal wound healing with subsequent maturation leading to regeneration or repair. However, persistently inflamed granulation tissue presents in osseous defects as a result of periodontitis and is routinely disrupted and discarded with non-surgical and surgical therapy to facilitate wound healing or improve chances of regeneration. Histological assessment suggests that granulation tissue from periodontitis-affected sites is effectively a chronic inflammatory tissue resulting from impaired wound healing due to persistence of bacterial dysbiotic bioflim. Nevertheless, the immunomodulatory potential and stem cell characteristics in granulation tissue have also raised speculation about the tissue's regenerative potential. This has led to the conception and recent implementation of surgical techniques which preserve granulation tissue with the intention of enhancing innate regenerative potential and improve clinical outcomes. As knowledge of fundamental cellular and molecular functions regulating periodontitis-affected granulation tissue is still scarce, this review aimed to provide a summary of current understanding of granulation tissue in the context of periodontal wound healing. This may provide new insights into clinical practice related to the management of granulation tissue and stimulate further investigation.

Humanistic Curriculum Model In Arabic Language Learning At Private MTs Yapena Lhokseumawe City

The objective of this research is to elucidate the humanistic curriculum development model and its utilization in crafting Arabic teaching resources. The research methodology employed is descriptive qualitative, adopting a case research approach at Private MTs of Yapena Lhokseumawe. The research encompasses several aspects: 1) understanding the concept of humanistic curriculum development, 2) implementing humanistic curriculum in Arabic teaching resources, 3) executing activities at Private MTs of Yapena, and 4) gathering insights through interviews and observations at Private MTs of Yapena Lhokseumawe. Data collection was conducted through observations and interviews with both teachers and students of Private MTs of Yapena. The collected data underwent analysis to condense and articulate outcomes derived from the fieldwork. The research revealed that the application of the humanistic curriculum development model in crafting Arabic teaching resources yielded favorable outcomes, enhancing both cognitive and non-cognitive abilities among students. By centering learning around students and providing them with opportunities to foster their innate potential and enhance creativity, the humanistic curriculum development model elicited a notable surge in student engagement, with a 60% increase in learning competencies observed post-research. Keywords: humanistic, curriculum development, Arabic language

Digital Twin and 3D Digital Twin: Concepts, Applications, and Challenges in Industry 4.0 for Digital Twin

The rapid development of digitalization, the Internet of Things (IoT), and Industry 4.0 has led to the emergence of the digital twin concept. IoT is an important pillar of the digital twin. The digital twin serves as a crucial link, merging the physical and digital territories of Industry 4.0. Digital twins are beneficial to numerous industries, providing the capability to perform advanced analytics, create detailed simulations, and facilitate informed decision-making that IoT supports. This paper presents a review of the literature on digital twins, discussing its concepts, definitions, frameworks, application methods, and challenges. The review spans various domains, including manufacturing, energy, agriculture, maintenance, construction, transportation, and smart cities in Industry 4.0. The present study suggests that the terminology “3 dimensional (3D) digital twin” is a more fitting descriptor for digital twin technology assisted by IoT. The aforementioned statement serves as the central argument of the study. This article advocates for a shift in terminology, replacing “digital twin” with “3D digital twin” to more accurately depict the technology’s innate potential and capabilities in Industry 4.0. We aim to establish that “3D digital twin” offers a more precise and holistic representation of the technology. By doing so, we underline the digital twin’s analytical ability and capacity to offer an intuitive understanding of systems, which can significantly streamline decision-making processes using the digital twin.

Abstract 83: NK cell ADCC assays: Leveraging flow cytometry and reporter cell lines for enhanced biological relevance and throughput

Abstract Amid the growing arsenal of cancer treatments, antibody-dependent cellular cytotoxicity (ADCC) assays have emerged as a significant contributor to the quest for more effective therapies. The ADCC-based antibody therapy often exploits Natural Killer (NK) cells as effectors. This immunotherapeutic approach harnesses the innate cytotoxic potential of NK cells, offering a promising solution for the precision targeting of malignant cells. In this study, we employed two methods that can be used to measure ADCC potency and specificity during preclinical drug discovery. The first was a flow cytometry-based approach that focused on enhancing biological relevance by utilizing primary human peripheral blood mononuclear cells (PBMCs) as a source for NK effector cells. To this end, target cells were fluorescently labeled with CFSE and cultured with freshly isolated PBMCs at 3 effector to target (E:T) ratios, in the presence of ADCC antibody. After 16h incubation, a viability dye was added, and percent cytotoxicity was measured in CFSE+ targets cells by flow cytometry. The second method offered a high-throughput plate reader-based approach (ADCC Reporter Bioassay; Promega), that utilized an ADCC reporter effector cell line harboring a luciferase cassette driven by an NFAT promoter, which is activated when effector and targets cells are bridged by a therapeutic antibody. Cytotoxicity was read out by measuring light output on a Cytation 3 plate imaging reader (Agilent Technologies) after a 6h cell culture incubation using the recommended 6:1 E:T ratio. Preclinical application for both assays was demonstrated using an anti-HER2 therapeutic antibody (trastuzumab) against BT-474, which is a HER2 positive cell line. To demonstrate specificity, an anti-VEGF antibody (bevacizumab) and Hs 578T cells (HER2 low) were used as controls. Our results revealed trastuzumab induced a strong dose-dependent increase in cytotoxicity in the BT-474 cells but not the Hs 578T cell line (range: 40-70%) while bevacizumab failed to induce any cytotoxicity above background levels at any concentration. Furthermore, the flow cytometry and plate reader methodologies produced similar trends. In summary, both applications can be used to produce reliable ADCC drug screening assessments. Additional considerations include investigational needs surrounding high throughput capability versus biological relevance. Unlike traditional models using immortalized cell lines, primary human PBMCs more faithfully recapitulate the physiological interactions between effector cells and target cells. On the other hand, the more streamlined reporter cell line approach not only accelerates assay throughput but also ensures consistent and reliable results in an attractive option for large-scale screening and drug development efforts. Citation Format: David William Draper, Yewei Xing, Scott Wise. NK cell ADCC assays: Leveraging flow cytometry and reporter cell lines for enhanced biological relevance and throughput [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 83.

The Objectives of Moral Education on Islamic View

Humans in the view of Islam have good innate potential. In the view of Islam, this potential is called fithrah. Humans since being born already have the potential for good and kindness. But in living his life, when humans relate to something outside themselves, due to environmental influences, humans who previously had good potential, became bad behavior and had bad morals.. The research used a qualitative approach with a literature study method. The research findings is moral education simply has the aim of improving student behavior and instilling it deeply so that the urge to have good behavior remains embedded in him. Humans have good innate potential (fithrah). Education as an effort to humanize humans is carried out to deliver humans to be as before, in a good state. Humans who return to the state of fithrah are humans with good morals. The process of moral education has the aim that humans become good human beings. In praxis, educational institutions not only prepare students to have certain intelligence and skills. Creating a good relationship between individuals and their God, both sirri (secretly) and a'lany (openly), makes individuals istikamah close to Allah Swt as if they see Him, feel His presence, and are sincere in worship.

Advances in 3D Culture Models to Study Exosomes in Triple-Negative Breast Cancer.

Breast cancer, a leading cause of cancer-related deaths globally, exhibits distinct subtypes with varying pathological, genetic, and clinical characteristics. Despite advancements in breast cancer treatments, its histological and molecular heterogeneity pose a significant clinical challenge. Triple-negative breast cancer (TNBC), a highly aggressive subtype lacking targeted therapeutics, adds to the complexity of breast cancer treatment. Recent years have witnessed the development of advanced 3D culture technologies, such as organoids and spheroids, providing more representative models of healthy human tissue and various malignancies. These structures, resembling organs in structure and function, are generated from stem cells or organ-specific progenitor cells via self-organizing processes. Notably, 3D culture systems bridge the gap between 2D cultures and in vivo studies, offering a more accurate representation of in vivo tumors' characteristics. Exosomes, small nano-sized molecules secreted by breast cancer and stromal/cancer-associated fibroblast cells, have garnered significant attention. They play a crucial role in cell-to-cell communication, influencing tumor progression, invasion, and metastasis. The 3D culture environment enhances exosome efficiency compared to traditional 2D cultures, impacting the transfer of specific cargoes and therapeutic effects. Furthermore, 3D exosomes have shown promise in improving therapeutic outcomes, acting as potential vehicles for cancer treatment administration. Studies have demonstrated their role in pro-angiogenesis and their innate therapeutic potential in mimicking cellular therapies without side effects. The 3D exosome model holds potential for addressing challenges associated with drug resistance, offering insights into the mechanisms underlying multidrug resistance and serving as a platform for drug screening. This review seeks to emphasize the crucial role of 3D culture systems in studying breast cancer, especially in understanding the involvement of exosomes in cancer pathology.

Both high and low dispersal? Apparently contradictory genetic patterns in the Antarctic littorinid gastropod Laevilacunaria antarctica

How the near-shore marine benthic communities survived Quaternary glaciations in Antarctica is a major question for Southern Ocean biogeographers. Several hypotheses that consider life-history traits, such as bathymetric ranges and developmental modes, have been proposed. Near-shore species with high dispersive potential are expected to show star-like genealogies, with broadly distributed haplotypes surrounded by closely related variants at low frequencies, a consequence of rapid population post-glacial expansion mediated by larvae. By contrast, shallow-water taxa with low dispersal potential are expected to evidence marked genetic structure or even consist of separate cryptic species, due to their survival in small refugial populations subject to repeated bottlenecks. In this study we performed phylogeographic analyses on the high-latitude littorinid Laevilacunaria antarctica across its distribution from maritime Antarctica to South Georgia. This species is a highly abundant near-shore gastropod, commonly found on macroalgae, with low autonomous vagility and exhibits benthic protected development with no free-living dispersive stages. Such characteristics make this species a suitable model to test the above hypotheses. Contrary to low dispersal potential predictions, L. antarctica comprises a single unstructured unit across its distribution in maritime Antarctica, exhibiting a typical high-dispersal star-like, short-branched genealogy centered on a dominant broadly distributed haplotype. The South Georgia population, however, consists of a separate genetic unit, strongly differentiated from those of the maritime Antarctic. We estimate that these two genetic groups separated ~1.2 My, long before the Last Glacial Maximum and evolved independently in these areas. Our results thus provide partial support for the expected pattern for a near-shore marine benthic species with low innate dispersive potential. On a wider geographic scale (maritime Antarctica vs South Georgia), our results in L. antarctica provide support for the expected pattern for a near-shore marine benthic species with low innate dispersive potential. However, on a narrower scale (maritime Antarctica) life-history characters including the close association of the species with macroalgae, would allow dispersal and a species with low dispersal potential exhibits paradoxically an absence of genetic structure across hundreds of kilometers probably mediated by rafting.

Astrocytic TIMP-1 regulates production of Anastellin, an inhibitor of oligodendrocyte differentiation and FTY720 responses

Astrocyte activation is associated with neuropathology and the production of tissue inhibitor of metalloproteinase-1 (TIMP1). TIMP1 is a pleiotropic extracellular protein that functions both as a protease inhibitor and as a growth factor. Astrocytes that lack expression of Timp1 do not support rat oligodendrocyte progenitor cell (rOPC) differentiation, and adult global Timp1 knockout (Timp1KO) mice do not efficiently remyelinate following a demyelinating injury. Here, we performed an unbiased proteomic analysis and identified a fibronectin-derived peptide called Anastellin (Ana) that was unique to the Timp1KO astrocyte secretome. Ana was found to block rOPC differentiation in vitro and enhanced the inhibitory influence of fibronectin on rOPC differentiation. Ana is known to act upon the sphingosine-1-phosphate receptor 1, and we determined that Ana also blocked the pro-myelinating effect of FTY720 (or fingolimod) on rOPC differentiation in vitro. Administration of FTY720 to wild-type C57BL/6 mice during MOG35-55-experimental autoimmune encephalomyelitis ameliorated clinical disability while FTY720 administered to mice lacking expression of Timp1 (Timp1KO) had no effect. Analysis of Timp1 and fibronectin (FN1) transcripts from primary human astrocytes from healthy and multiple sclerosis (MS) donors revealed lower TIMP1 expression was coincident with elevated FN1 in MS astrocytes. Last, analyses of proteomic databases of MS samples identified Ana peptides to be more abundant in the cerebrospinal fluid (CSF) of human MS patients with high disease activity. A role for Ana in MS as a consequence of a lack of astrocytic TIMP-1 production could influence both the efficacy of fingolimod responses and innate remyelination potential in the MS brain.

Immunomodulatory effects of epiphytic Loranthus micranthus leaf extracts collected from two host plants: Psidium guajava and Parkia biglobosa

BackgroundImmunological abnormalities are implicated in the pathogenesis of many chronic diseases. Due to the drug-related adverse effects of currently available orthodox immunomodulators, natural immunomodulators are being looked upon as potential agents to replace them in therapeutic regimens. This research aimed to investigate the immunomodulatory potential of L. micranthus extracts epiphytic on Psidium guajava (LMPGE) and Parkia biglobosa (LMPBE).MethodsPhytochemical screening and acute toxicity testing were carried out to identify the phytoconstituents and safety profiles of the extracts. The extracts’ innate and adaptive immunomodulatory potentials were determined in experimental animals using in vivo leucocyte mobilization, delayed-type hypersensitivity (DTH) response, hemagglutination antibody titre, and cyclophosphamide-induced myelosuppression models. Levamisole was used as the standard drug throughout the study.ResultsCompared to LMPBE, LMPGE contained significantly (p < 0.05) more tannins, cyanogenic glycosides, saponins, reducing sugars, glycosides, flavonoids, and alkaloids. Furthermore, the groups treated with the extracts had a significant (p < 0.05) increase in the total number of leucocytes, neutrophils, basophils, and antibody titers relative to the untreated control. In the same way, the treatment raised TLC in cyclophosphamide-intoxicated rats, with 250 mg/kg b. w. of LMPGE and LMPBE recording 9712.50 ± 178.00 and 8000.00 ± 105.00 × 109 /L, respectively, compared to 3425.00 ± 2 5.00 × 109 /L in the untreated group. Overall, LMPGE was more effective.ConclusionsThe findings from this study suggest that L. micranthus epiphytic in Psidium guajava and Parkia biglobosa has possible immune stimulating potential.

THE SECRETOME OF AEC: CHALLENGES AND OPPORTUNITIES IN CELL-FREE REGENERATIVE MEDICINE IN TENDON DISORDERS

The application of immune regenerative strategies to deal with unsolved pathologies, such as tendinopathies, is getting attention in the field of tissue engineering exploiting the innate immunomodulatory potential of stem cells [1]. In this context, Amniotic Epithelial Cells (AECs) represent an innovative immune regenerative strategy due to their teno-inductive and immunomodulatory properties [2], and because of their high paracrine activity, become a potential stem cell source for a cell-free treatment to overcome the limitations of traditional cell-based therapies. Nevertheless, these immunomodulatory mechanisms on AECs are still not fully known to date. In these studies, we explored standardized protocols [3] to better comprehend the different phenotypic behavior between epithelial AECs (eAECs) and mesenchymal AECs (mAECs), and to further produce an enhanced immunomodulatory AECs-derived secretome by exposing cells to different stimuli. Hence, in order to fulfill these aims, eAECs and mAECs at third passage were silenced for CIITA and Nrf2, respectively, to understand the role of these molecules in an inflammatory response. Furthermore, AECs at first passage were seeded under normal or GO-coated coverslips to study the effect of GO on AECs, and further exposed to LPS and/or IL17 priming to increase the anti-inflammatory paracrine activity. The obtained results demonstrated how CIITA and Nrf2 control the immune response of eAECs and mAECs, respectively, under standard or immune-activated conditions (LPS priming). Additionally, GO exposition led to a faster activation of the Epithelial-Mesenchymal transition (EMT) through the TGFβ/SMAD signaling pathway with a change in the anti-inflammatory properties. Finally, the combinatory inflammatory stimuli of LPS+IL17 enhanced the paracrine activity and immunomodulatory properties of AECs. Therefore, AECs-derived secretome has emerged as a potential treatment option for inflammatory disorders such as tendinopathies.Acknowledgement: This research is part of the P4FIT project ESR1, funded under the H2020-ITN-EJD-Marie-Skłodowska-Curie grant agreement 955685.

Measuring The Effectiveness of Youth Development Training Provided by Bangladesh National Cadet Corps: A Case Study

ABSTRACT: Youth represent a pivotal demographic and social asset for every nation, serving as a critical component for national human resource development. Strategic investment in systematic and well-planned Youth Development Training (YDT) can unlock their innate potential and equip them to navigate career challenges effectively. The Bangladesh National Cadet Corps (BNCC) offers YDT as a significant intervention for youth development, potentially fostering both individual growth and the sustainable progress of Bangladesh. This study aims to assess the effectiveness of BNCC’s YDT in developing leadership qualities, personal skills, and community development attitudes through quantitative analysis. An extensive literature review was meticulously undertaken, investigating deep into existing literature to identify the research gap areas, develop hypotheses, and carefully place the relevant variables essential for the study’s comprehensive analysis and interpretation. A survey instrument comprising sixteen variables was devised, employing the widely used five-point rating scale to assess participant responses. Primary data were collected from 170 male and female BNCC cadets. However, data analysis proceeded utilizing the one-sample “t-test” method. 14 out of 16 hypotheses were accepted with an admirable score. The findings of the study indicate that BNCC’s YDT significantly enhances leadership qualities, particularly in command skills and self-confidence among cadets. Additionally, the YDT program demonstrates a notable impact on fostering community development attitudes among participants. However, the YDT of BNCC is relatively poor in developing creative capability and Information Technology (IT) Skills among the cadets. Based on these results, specific policy recommendations are proposed, including heightened promotion of BNCC training and social development activities to its stakeholders, the establishment of leadership development institutes and innovation clubs, a memorandum of understanding with social service agencies to facilitate social development activities, and many more to maintain the area of strength, which could also contribute to remodeling the existing theories of youth development.

Kashi Tripti: A newly developed high-yielding mid-season edible-podded variety of vegetable pea

The COVID-19 epidemic has highlighted the crucial need to live a healthy lifestyle, particularly in metropolitan areas. As a result, there has been an increase in demand for functional foods that are high in important nutrients and support excellent health. Pea (Pisum sativum L.) is one of the oldest multipurpose legumes grown for human food, animal fodder, and ornamental and aids in ecosystem balance due to their innate nitrogen fixation potential (Devi et al., 2019; Devi et al., 2023). They are grown over an area of 7.04 and 2.59 million hectares for dry and green seeds, respectively (FAOSTAT, 2021). Peas have a beautiful balance of micro and macro nutrition profiles along with high dietary fiber, antioxidants and numerous important biomolecules, thus extremely useful in curing diabetes, cardio problems, certain cancers and many degenerative diseases (Kalloo et al., 2002; Kumari &amp; Deka, 2021; Sharma et al., 2023).