Innate Healing Research Articles

Enhancing Wound Healing With Sprayable Hydrogel Releasing Multi Metallic Ions: Inspired by the Body's Endogenous Healing Mechanism.

In the pursuit of new wound care products, researchers are exploring methods to improve wound healing through exogenous wound healing products. However, diverging from this conventional approach, this work has developed an endogenous support system for wound healing, drawing inspiration from the body's innate healing mechanisms governed by the sequential release of metal ions by body at wound site to promote different stages of wound healing. This work engineers a multi-ion-releasing sprayable hydrogel system, to mimic this intricate process, representing the next evolutionary step in wound care products. It comprises Alginate (Alg) and Fibrin (Fib) hydrogel infused with Polylactic acid (PLA) polymeric microcarriers encapsulating multi (calcium, copper, and zinc) nanoparticles (Alg-Fib-PLA-nCMB). Developed sprayable Alg-Fib-PLA-nCMB hydrogel show sustained release of beneficial multi metallic ions at wound site, offering a range of advantages including enhanced cellular function, antibacterial properties, and promotion of crucial wound healing processes like cell migration, ROS mitigation, macrophage polarization, collagen deposition, and vascular regeneration. In a comparative study with a commercial product (Midstress spray), developed Alg-Fib-PLA-nCMB hydrogel demonstrates superior wound healing outcomes in a rat model, indicating its potential for next generation wound care product, addressing critical challenges and offering a promising avenue for future advancements in the wound management.

Self-adaptive bioactive scaffolds orchestrate diabetic microenvironment remodeling and vascularized bone regeneration

The repair of diabetic bone defects is still challenging as the innate healing process is impaired by adverse microenvironments such as excessive reactive oxygen species (ROS) and insufficient angiogenesis and osteogenesis. Herein, a self-adaptive biocomposite scaffold that can orchestrate sequential regulation of diabetic microenvironment and vascularized bone regeneration was developed for efficient diabetic bone defect repair. The therapeutic scaffold system was meticulously constructed through the incorporation of hydrogel and 3D-printed architecture. In response to the multiple diabetic microenvironment (high level of glucose and ROS), the hydrogel consisting of Vitamin C-loaded phenylboric acid-grafted poly (glutamic acid)/poly (vinyl alcohol) showed adaptive degradation and antioxidative properties by scavenging the intracellular ROS, as well as promoting M2 polarization of macrophages. Furthermore, the copper-doped bioactive glass-contained 3D-printed polycaprolactone within the scaffold system was demonstrated to enhance the mechanical strength and stimulate the in vitro angiogenic and osteogenic performance by releasing copper and silicon ions. Moreover, in a diabetic rat femoral defect model, the biocomposite scaffold showed better angiogenesis and bone regeneration by following diabetic microenvironment remodeling. Therefore, the designed self-adaptive biocomposite scaffold can promote diabetic bone regeneration by sequentially regulating pathological and regenerative cues. This study offers vital insight into the design of a self-adaptive bioactive scaffold for the treatment of diabetic bone defects.

Biohacking Nerve Repair: Novel Biomaterials, Local Drug Delivery, Electrical Stimulation, and Allografts to Aid Surgical Repair.

The regenerative capacity of the peripheral nervous system is limited, and peripheral nerve injuries often result in incomplete healing and poor outcomes even after repair. Transection injuries that induce a nerve gap necessitate microsurgical intervention; however, even the current gold standard of repair, autologous nerve graft, frequently results in poor functional recovery. Several interventions have been developed to augment the surgical repair of peripheral nerves, and the application of functional biomaterials, local delivery of bioactive substances, electrical stimulation, and allografts are among the most promising approaches to enhance innate healing across a nerve gap. Biocompatible polymers with optimized degradation rates, topographic features, and other functions provided by their composition have been incorporated into novel nerve conduits (NCs). Many of these allow for the delivery of drugs, neurotrophic factors, and whole cells locally to nerve repair sites, mitigating adverse effects that limit their systemic use. The electrical stimulation of repaired nerves in the perioperative period has shown benefits to healing and recovery in human trials, and novel biomaterials to enhance these effects show promise in preclinical models. The use of acellular nerve allografts (ANAs) circumvents the morbidity of donor nerve harvest necessitated by the use of autografts, and improvements in tissue-processing techniques may allow for more readily available and cost-effective options. Each of these interventions aid in neural regeneration after repair when applied independently, and their differing forms, benefits, and methods of application present ample opportunity for synergistic effects when applied in combination.

음양균형의학으로서의 턱관절균형의학 개요

Objectives Temporomandibular Joint Balancing Medicine (TBM) is a relatively new approach to the temporomandibular joint(TMJ) perceived as a key to the whole-body balance. This study aimed to briefly review concepts, history, and research reports on TBM with a focus on the yin-yang balance concept. Methods A narrative review was performed regarding TBM referring to published literature. Results TBM encompassed a variety of innovative clinical techniques for evaluation and improvement of the balance of the TMJ, nervous system, and meridian system featuring the integration between TMJ and body. Its target ranged from diseases to subclinical functional imbalances. TBM harnessed the postural balance of TMJ as a central tool to manipulate postural proprioception and thereby stimulate the innate healing mechanism of the whole body. Conclusions TBM was based on the concept of yin-yang balance and provided an integrated approach to disease treatment as well as to the improvement of subclinical health conditions.

Maintenance of homeostasis by TLR4 ligands.

Immunotherapy is renowned for its capacity to elicit anti-infective and anti-cancer effects by harnessing immune responses to microbial components and bolstering innate healing mechanisms through a cascade of immunological reactions. Specifically, mammalian Toll-like receptors (TLRs) have been identified as key receptors responsible for detecting microbial components. The discovery of these mammalian Toll-like receptors has clarified antigen recognition by the innate immune system. It has furnished a molecular foundation for comprehending the interplay between innate immunity and its anti-tumor or anti-infective capabilities. Moreover, accumulating evidence highlights the crucial role of TLRs in maintaining tissue homeostasis. It has also become evident that TLR-expressing macrophages play a central role in immunity by participating in the clearance of foreign substances, tissue repair, and the establishment of new tissue. This macrophage network, centered on macrophages, significantly contributes to innate healing. This review will primarily delve into innate immunity, specifically focusing on substances targeting TLR4.

Osteopathic manipulative treatment for chronic inflammatory diseases.

Chronic inflammatory diseases (CIDs) are debilitating and potentially lethal illnesses that affect a large proportion of the global population. Osteopathic manipulative treatment (OMT) is a manual therapy technique developed and performed by osteopathic physicians that facilitates the body's innate healing processes. Therefore, OMT may prove a beneficial anti-inflammatory modality useful in the management and treatment of CIDs. This work aims to objectively evaluate the therapeutic benefits of OMT in patients with various CIDs. In this review, a structured literature search was performed. The included studies involving asthma, chronic obstructive pulmonary disease, irritable bowel syndrome, ankylosing spondylitis, and peripheral arterial disease were selected for this work. Various OMT modalities, including lymphatic, still, counterstain, and muscle energy techniques, were utilized. Control treatments included sham techniques, routine care, or no treatment. OMT utilization led to variable patient outcomes in individuals with pathologies linked to CID.

Silk Fibroin and Its Nanocomposites for Wound Care: A Comprehensive Review.

For most individuals, wound healing is a highly organized, straightforward process, wherein the body transitions through different phases in a timely manner. However, there are instances where external intervention becomes necessary to support and facilitate different phases of the body's innate healing mechanism. Furthermore, in developing countries, the cost of the intervention significantly impacts access to treatment options as affordability becomes a determining factor. This is particularly true in cases of long-term wound treatment and management, such as chronic wounds and infections. Silk fibroin (SF) and its nanocomposites have emerged as promising biomaterials with potent wound-healing activity. Driven by this motivation, this Review presents a critical overview of the recent advancements in different aspects of wound care using SF and SF-based nanocomposites. In this context, we explore various formats of hemostats and assess their suitability for different bleeding situations. The subsequent sections discuss the primary causes of nonhealing wounds, i.e., prolonged inflammation and infections. Herein, different treatment strategies to achieve immunomodulatory and antibacterial properties in a wound dressing were reviewed. Despite exhibiting excellent pro-healing properties, few silk-based products reach the market. This Review concludes by highlighting the bottlenecks in translating silk-based products into the market and the prospects for the future.

Indications of Platelet-Rich Plasma (PRP) in Dermatology

Introduction: PRP is an autologous serum containing high concentrations of platelets and growth factors. It is used in dermatology for a variety of purposes, including hair growth, skin renewal, the removal of acne scars, dermal augmentation, and the treatment of striae distensae. The origins of PRP trace back to the 1970s, originating within the field of hematology as a treatment for patients grappling with thrombocytopenia. The subsequent decades, specifically the 1980s and 1990s, witnessed the integration of PRP into surgical procedures, notably in maxillofacial and plastic surgeries. The rationale underlying the employment and therapeutic potential of a concentrated platelet solution hinges upon their remarkable ability to furnish elevated levels of vital growth factors, thus instigating a regenerative impetus that facilitates repair in tissues characterized by limited innate healing capabilities. This comprehensive review delves into the multifaceted facets of PRP, encompassing mechanisms of action, prevailing indications, existing clinical substantiation, safety considerations, and the burgeoning spectrum of potential applications within the domain of skin condition treatments.
 Aim of the study: To review the current literature on the use of PRP in the field of dermatology
 Materials and Methods: We reviewed the literature available in PubMed, using the key words “platelet rich plasma”, “prp”, “autologous platelet rich plasma”, “alopecia”, “anti-aging”
 Results: PRP has a lot of potential in dermatology and its therapeutic uses are growing. Future research should standardize PRP therapy procedures for particular reasons.
 Summary: The versatile action of PRP has made it the subject of intense research. However, the reports in the literature to date on its use require further studies on larger groups of people to confirm its effects.

Extracellular Matrix/Glycopeptide Hybrid Hydrogel as an Immunomodulatory Niche for Endogenous Cardiac Repair after Myocardial Infarction.

The treatment of myocardial infarction (MI) remains a substantial challenge due to excessive inflammation, massive cell death, and restricted regenerative potential, leading to maladaptive healing process and eventually heart failure. Current strategies of regulating inflammation or improving cardiac tissue regeneration have limited success. Herein, a hybrid hydrogel coassembled by acellular cardiac extracellular matrix (ECM) and immunomodulatory glycopeptide is developed for endogenous tissue regeneration after MI. The hydrogel constructs a niche recapitulating the architecture of native ECM for attracting host cell homing, controlling macrophage differentiation via glycopeptide unit, and promoting endotheliocyte proliferation by enhancing the macrophage-endotheliocyte crosstalk, which coordinate the innate healing mechanism for cardiac tissue regeneration. In a rodent MI model, the hybrid hydrogel successfully orchestrates a proreparative response indicated by enhanced M2 macrophage polarization, increased angiogenesis, and improved cardiomyocyte survival, which alleviates infarct size, improves wall thicknesses, and enhances cardiac contractility. Furthermore, the safety and effectiveness of the hydrogel are demonstrated in a porcine MI model, wherein proteomics verifies the regulation of immune response, proangiogenesis, and accelerated healing process. Collectively, the injectable composite hydrogel serving as an immunomodulatory niche for promoting cell homing and proliferation, inflammation modulation, tissue remodeling, and function restoration provides an effective strategy for endogenous cardiac repair.

Making sense of complexity: A qualitative ‘Framework’ analysis of naturopathic case management and clinical reasoning

Background and purposeThe clinical encounter is inherently complex and uncertain. Naturopathic clinical practice is shaped by a traditional philosophy and practice guiding principles, with a therapeutic framework that incorporates a complex inter-systems approach. It is possible that this foundation may orient naturopathic practitioners to manage clinical complexity and uncertainty in a distinct manner. The aim of this study is to explore the perceptions of experienced naturopathic practitioners to the management of clinical complexity within naturopathic care. Materials and methodsTwenty experienced Australian naturopathic practitioners participated across four focus groups, responding to semi-structured questions regarding their clinical reasoning strategies and case management processes. The data were analysed using a seven step Framework analysis method. ResultsThree primary themes were identified: i) patient is encountered as a whole entity, ii) clinical reasoning is ampliative and explicative, and iii) treatment reflects systems thinking. Participants perceived a focus on the connections between various case elements, building a comprehensive internalised schematic of each case. Participants saw treatments as meeting various needs including prevention, symptom alleviation, causal mitigation, and support of innate healing processes. ConclusionNaturopathic practitioners perceive they clinically embody the traditional holistic philosophy of naturopathy as a systems orientation, incorporating traditional and contemporary bioscience knowledge. This appears to shape a distinct naturopathic case management approach, oriented to working with patients in a complexity-informed manner.

IPSC-derived tenocytes seeded on microgrooved 3D printed scaffolds for Achilles tendon regeneration.

Tendons and ligaments have a poor innate healing capacity, yet account for 50% of musculoskeletal injuries in the United States. Full structure and function restoration postinjury remains an unmet clinical need. This study aimed to assess the application of novel three dimensional(3D) printed scaffolds and induced pluripotent stem cell-derived mesenchymal stem cells (iMSCs) overexpressing the transcription factor Scleraxis (SCX, iMSCSCX+ ) as a new strategy for tendon defect repair. The polycaprolactone (PCL) scaffolds were fabricated by extrusion through a patterned nozzle or conventional round nozzle. Scaffolds were seeded with iMSCSCX+ and outcomes were assessed in vitro via gene expression analysis and immunofluorescence. In vivo, rat Achilles tendon defects were repaired with iMSCSCX+ -seeded microgrooved scaffolds, microgrooved scaffolds only, or suture only and assessed via gait, gene expression, biomechanical testing, histology, and immunofluorescence.iMSCSCX+ -seeded on microgrooved scaffolds showed upregulation of tendon markers and increased organization and linearity of cells compared to non-patterned scaffolds in vitro. In vivo gait analysis showed improvement in the Scaffold + iMSCSCX+ -treated group compared to the controls. Tensile testing of the tendons demonstrated improved biomechanical properties of the Scaffold + iMSCSCX+ group compared with the controls. Histology and immunofluorescence demonstrated more regular tissue formation in the Scaffold + iMSCSCX+ group. This study demonstrates the potential of 3D-printed scaffolds with cell-instructive surface topography seeded with iMSCSCX+ as an approach to tendon defect repair. Further studies of cell-scaffold constructs can potentially revolutionize tendon reconstruction by advancing the application of 3D printing-based technologies toward patient-specific therapies that improve healing and functional outcomes at both the cellular and tissue level.

African Spiny Mice (Acomys) Exhibit Mild Osteoarthritis Following Meniscal Injury.

Hyaline cartilage has limited innate healing abilities and hyaline cartilage loss is a hallmark of osteoarthritis (OA). Animal models can provide important insights into cartilage regeneration potential. One such animal model, the African spiny mouse (Acomys), is capable of regenerating skin, skeletal muscle, and elastic cartilage. This study aims to evaluate whether these regenerative abilities protect Acomys with meniscal injury from OA-related joint damage and behaviors indicative of joint pain and dysfunction. Acomys received destabilization of the medial meniscus (DMM) surgery (n = 11) or a skin incision (n = 10). Gait testing occurred at 4, 6, 8, 10, and 12 weeks after surgery. At endpoint, joints were processed for histology to assess cartilage damage. Following joint injury, Acomys with DMM surgery altered their walking patterns by increasing the percent stance time on the contralateral limb relative to the operated limb, thereby reducing the amount of time the injured limb must bear weight on its own throughout the gait cycle. Histological grading indicated evidence of OA-related joint damage in Acomys with DMM surgery; these changes were primarily driven by loss of structural integrity in the hyaline cartilage. Acomys developed gait compensations, and the hyaline cartilage in Acomys is not fully protected from OA-related joint damage following meniscal injury, although this damage was less severe than that historically found in C57BL/6 mice with an identical injury. Thus, Acomys do not appear to be completely protected from OA-related changes, despite the ability to regenerate other wounded tissues.

Oxygen-supplying syringe to create hyperoxia-inducible hydrogels for in situ tissue regeneration

Recent trends in the design of regenerative materials include the development of bioactive matrices to harness the innate healing ability of the body using various biophysicochemical stimuli (defined as in situ tissue regeneration). Among these, hyperoxia (>21% pO2) is a well-known therapeutic factor for promoting tissue regeneration, such as immune cell recruitment, cell proliferation, angiogenesis, and fibroblast differentiation into myofibroblast. Although various strategies to induce hyperoxia are reported, developing advanced hyperoxia-inducing biomaterials for tissue regeneration is still challenging. In this study, a catalase-immobilized syringe (defined as an Oxyringe) via calcium peroxide-mediated surface modification is developed as a new type of oxygen-supplying system. Hyperoxia-inducible hydrogels are fabricated utilizing Oxyringe. This hydrogel plays a role as a physical barrier for hemostasis. In addition, hyperoxic matrices induce transient hyperoxia in vivo (up to 46.0% pO2). Interestingly, the hydrogel-induced hyperoxia boost the initial macrophage recruitment and rapid inflammation resolution. Furthermore, hyperoxic oxygen release of hydrogels facilitates neovascularization and cell proliferation involved in the proliferation phase, expediting tissue maturation related to the remodeling phase in wound healing. In summary, Oxyringe has excellent potential as an advanced oxygen-supplying platform to create hyperoxia-inducing hydrogels for in situ tissue regeneration.

A Review of the Therapeutic Potentials of Stem Cells, Fibroblast Growth Factors and T-cells in Regenerative Medicine

The human body is a complex structure with the innate ability to protect, defend, repair, and heal after damage or disease. For decades, medicine has faced problems that need the evolution of standard treatments and finding a way to accelerate the regenerative capabilities of the body, which possibly would not just treat but also cure certain diseases that previously had no cure. The question researchers have pondered on is whether or not it was possible as humans to use the body's innate healing power to our advantage and clinically accelerate or modify it to upgrade the treatment of certain diseases. The answer they found was in Regenerative Medicine (RM).
 Historically the term regenerative medicine was found for the first time in a paper published in 1992 by Leland Kaiser. He made a list of approaches that would impact the future of hospitals. However, it is widely believed to have been coined during a 1999 conference on Lake Como by William Haseltine in an attempt to describe a novel field of medicine that combined knowledge from subjects like cell transplant, biochemistry, nanotechnology, prosthetics biomechanics, tissue engineering, and stem cell biology.
 Regenerative Medicine is a relatively new field of clinical applications and research that is focused on the development of therapies like tissue engineering and stem cell technologies to repair, regenerate or replace defective, aged, injured, diseased, or permanently damaged organs, tissues or cells in order to restore them to their normal function.
 It is important to note that RM has rapidly become one of the top treatment options for acute and chronic injuries, congenital diseases, and a wide range of acute and chronic diseases. It is more than just a field of medicine involving basic replacement therapies or traditional transplantation; it applies approaches like gene therapy, reprogramming of cell types, stem cell transplantation, and the use of soluble molecules, tissue engineering, and lots more.
 The review article focuses on the therapeutic effects of fibroblast growth factors (FGFs), adipose-derived stem cells (ADSCs), and regulatory T cells (Tregs), and the possible role they play in tissue regeneration. They are apparently useful in the treatment of myriads of diseases expectedly having no cure.

Thermoresponsive and Injectable Hydrogel for Tissue Agnostic Regeneration.

Injectable hydrogels can support the body's innate healing capability by providing a temporary matrix for host cell ingrowth and neovascularization. The clinical adoption of current injectable systems remains low due to their cumbersome preparation requirements, device malfunction, product dislodgment during administration, and uncontrolled biological responses at the treatment site. To address these challenges, a fully synthetic and ready-to-use injectable biomaterial is engineered that forms an adhesive hydrogel that remains at the administration site regardless of defect anatomy. The product elicits a negligible local inflammatory response and fully resorbs into nontoxic components with minimal impact on internal organs. Preclinical animal studies confirm that the engineered hydrogel upregulates the regeneration of both soft and hard tissues by providing a temporary matrix to support host cell ingrowth and neovascularization. In a pilot clinical trial, the engineered hydrogel is successfully administered to a socket site post tooth extraction and forms adhesive hydrogel that stabilizes blood clot and supports soft and hard tissue regeneration. Accordingly, this injectable hydrogel exhibits high therapeutic potential and can be adopted to address multiple unmet needs in different clinical settings.

Flowable placental connective tissue matrices for tendon repair: A review

Tendon injuries are associated with considerable pain and disability. Owing to the hypovascularity and hypocellularity of the tissue, natural tendon healing is slow and ineffective. Traditional conservative and surgical treatment options fail to address the underlying pathology. As a result, the healed tendon is mechanically incompetent and prone to degeneration and rupture. Therefore, new biological methods have been suggested to enhance tendon repair and regeneration. Flowable Placental Connective Tissue Matrices (FP-CTMs) represent a promising means to promote tendon healing. Like non-flowable placental scaffolds, FP-CTMs possess the innate healing properties of the placenta and provide structural and biochemical extracellular matrix components. Unlike their non-flowable counterparts, FP-CTMs have the added benefits of minimal invasiveness and the capacity to fill irregular spaces. FP-CTMs can enhance tendon repair by providing a three-dimensional extracellular matrix for cellular attachment and proliferation while decreasing inflammation and limiting adhesion formation. The present report reviews tendon biology, pathology, healing, and current treatment modalities, followed by a comprehensive literature review, evaluating the clinical application of FP-CTMs for tendon repair. Recent research suggests that the use of FP-CTMs in tendon repair is safe and efficacious and further indicates that FP-CTMs can modulate the tendon repair environment and improve clinical outcomes. However, the existing clinical evidence is limited to retrospective case series with no control group. Therefore, additional work must be performed to better understand the clinical applications and therapeutic benefits of FP-CTM in tendon repair compared with conventional treatments.

Overview on scope and advancement in regenerative medicine

Regenerative medicine is the science of replacing engineering, regenerating human cells, tissues, or organs to restore or establish normal function and has now reached its potential due to its ability to deliver cell and tissue constructs to the human body thus providing therapeutic assistance of its innate healing responses. It further comprehends the cellular and molecular differences between regenerating and non-regenerating tissues thus resulting in restoring tissue structure and function in damaged, non-regenerating tissues. Replaced or repaired tissues should be indistinguishable from normal, healthy tissues in structure and function. Cellular therapy is one such strategy wherein various cell types are isolated and used that help in understanding their mechanism of action as well as their regenerative properties when they are utilized with different biomaterials as an extracellular matrix. As scaffolds provide targeted cell growth, stem cell delivery through scaffolding technology is another regenerative therapy that is used. The process of electrospinning is used for the generation of scaffolds. Problems like the unavailability of organs for transplantation prevails and thus regenerative medicine therapy has proved to be applicable in such cases.

Multifunctional and Smart Wound Dressings-A Review on Recent Research Advancements in Skin Regenerative Medicine.

The healing of wounds is a dynamic function that necessitates coordination among multiple cell types and an optimal extracellular milieu. Much of the research focused on finding new techniques to improve and manage dermal injuries, chronic injuries, burn injuries, and sepsis, which are frequent medical concerns. A new research strategy involves developing multifunctional dressings to aid innate healing and combat numerous issues that trouble incompletely healed injuries, such as extreme inflammation, ischemic damage, scarring, and wound infection. Natural origin-based compounds offer distinct characteristics, such as excellent biocompatibility, cost-effectiveness, and low toxicity. Researchers have developed biopolymer-based wound dressings with drugs, biomacromolecules, and cells that are cytocompatible, hemostatic, initiate skin rejuvenation and rapid healing, and possess anti-inflammatory and antimicrobial activity. The main goal would be to mimic characteristics of fetal tissue regeneration in the adult healing phase, including complete hair and glandular restoration without delay or scarring. Emerging treatments based on biomaterials, nanoparticles, and biomimetic proteases have the keys to improving wound care and will be a vital addition to the therapeutic toolkit for slow-healing wounds. This study focuses on recent discoveries of several dressings that have undergone extensive pre-clinical development or are now undergoing fundamental research.

Trauma as Absence: A Biopsychosocial-AIP Definition of Trauma and Its Treatment in EMDR Therapy

This paper advances the biopsychosocial adaptive information processing (BPS-AIP) model and theory (Cotraccia, 2012) by adding consciousness as a mechanism of action activated via social behavior in EMDR. An attention schema is conceptualized as content integrated with dynamic self-models that maintain subjective mental states of biopsychosocial connectivity or disconnectivity. These implicit self-models are portrayed as determining 1) the disposition of the BPS-AIP system of clients presenting for EMDR as more or less connected or disconnected prior to, at the time of, and following, a stressful event; and 2) whether or not stressful events become traumatizing over time.Attention schema theory(Graziano, 2021) is integrated to further describe how establishing and enhancing attentional agency when addressing simple and complex-PTSD facilitates consolidation of autobiographical memory. Self-integration in the context of an attuned therapeutic relationship is described in terms of patterns of tracking attention to promote heightened states of self/other consciousness and activate the innate healing system. Attention schema theory, as a prominent theory of consciousness, adds to BPS-AIP’s compelling description of how an innate neurophysiological system can be integrated with psychosocial components that explain how both subjective and intersubjective experiences are causally relevant to the processing of autobiographical memory.

Immunomodulatory Effect of SINA 1.2 Therapy Protocol in Asthmatic Mice Model: A Combination of Oxymel and Sauna.

Alternative medicine, has become popular in asthmatic patients. We evaluated the immunomodulatory effects of SINA 1.2 therapy protocol derived from Persian medicine in an asthmatic mice model. Forty-two male BALB/c mice divided into six groups: one control (sham) and five sensitized groups (by parenteral injection of 20 μg ovalbumin in 100 μL normal saline plus 50 μL alum on days 1 and 14). Sensitized groups were as: untreated, budesonide (1 mg nebulized budesonide: 200 μg/puff every 5 min for 25 min), dry sauna (30 min, 37°C), oral oxymel (gavaged: 0.2 mL of the syrup plus 0.8 mL of water), and SINA protocol No.1.2 (oxymel followed by sauna) groups. Treatments were given for 10 days from day 23 to 33 then sacrificed. Significant gene expression reduction of interleukin(IL)-4, IL-5, and MUC5AC and increase of interferon(IFN)-γ and IFN-γ/IL-4 ratio and decreased perivascular and peribronchial inflammation, goblet cell hyperplasia, and subsequent mucus hypersecretion in SINA group were seen compared to untreated group. SINA lowered IL-5 and MUC5AC gene expression levels similar to the budesonide and acted better than budesonide in increasing IFN-γ gene expression up to normal level. Compared with the asthma group, sauna alone only affected MUC5AC and IFN-γ gene expressions and oxymel alone, only reduced IL-4 gene expression, perivascular and peribronchial inflammation, and mucus hypersecretion. It seems that SINA therapy alleviates asthma via immune modulation of pro-inflammatory cytokines and improvement of pathological changes in ovalbumin-induced asthma in mice, supporting the notion of innate healing power mentioned in Persian medicine literature.