Injection Of Acid Research Articles

Comparative Bulk RNA-Seq Analysis of Poly-l-Lactic Acid Versus Calcium Hydroxylapatite Reveals a Novel, Adipocyte-Mediated Regenerative Mechanism of Action Unique to PLLA.

Injectable biostimulators are widely used to improve facial aging signs. This study was performed to compare the genetic pathways affected by 2 different injectable biostimulators. Randomized 13-week study (n = 21) comparing gene expression after poly l-lactic acid (PLLA-SCA) and calcium hydroxylapatite (CaHA-R) injections (baseline [BL] and Day 28) as treatment of nasolabial fold wrinkles. Punch biopsy was performed at BL and day 90; RNA was isolated, gene expression was analyzed, and bulk RNA sequencing performed. Data from both pathways were studied using the Search Tool for Retrieval of Interacting Genes/Proteins and Reactome databases. Analysis at Day 0 and Day 90 showed differing gene regulation for PLLA-SCA and CaHA-R (after accounting for minor differences at BL, p < .05 at Day 90 for 9-12 genes). PLLA-SCA uniquely correlates with genes involved in adipocyte regeneration, while CaHA-R did not affect these genes. Clinically, this could translate to healthy fat replacement with an enhanced effect beyond the improvement of the collagen structure and a longer-lasting effect with PLLA-SCA. Furthermore, the adipokines that appear to be upregulated are regenerative with a positive impact on skin health. PLLA-SCA has a novel and unique signature, which supports a potential regenerative mechanism of action through its role in the modulation of adipocyte function.

Effect of repeated intra-articular administration of collagen as a treatment for knee osteoarthritis: a case report.

Intra-articular injections aimed at correcting underlying pathophysiological processes and providing pain relief are essential for managing knee osteoarthritis (KOA). Collagen, the primary component of articular cartilage, has a long half-life, making it a promising candidate for intra-articular injections with a low risk of serious side effects. The first of the two cases in this report involved a woman in her early 70s with a 6-year history of persistent left knee pain. The second case involved a woman in her mid-50 s with a >7-year history of right knee pain. Both patients received hyaluronic acid injections every 6 months, totaling 10 injections over 5 years. They were diagnosed with Kellgren-Lawrence grade 2 KOA. The patients received two 3-mL intra-articular injections of 6% collagen, administered at baseline and 6 months later. Improvements in clinical outcomes, including visual analog scale scores and Western Ontario and McMaster Universities Osteoarthritis Index scores, were observed and maintained in both patients, with no side effects reported. In summary, when collagen injections were administered to these two patients with KOA, clinical improvements lasted approximately 6 months, and repeated treatments demonstrated efficacy and safety similar to the initial course.

Important role of relationship between brain and spleen in the mechanisms of chronic pain development and maintenance in fibromyalgia

Fibromyalgia (FM) is characterized by chronic generalized pain accompanied by various symptoms, such as extreme fatigue, insomnia and depression. Clinical studies have indicated the presence of psychological stress, sympathetic nervous system hyperexcitation and immune system abnormalities, as a trigger for the onset of the disease, but the contribution to the pathogenesis of the disease remains unclear. Here, we employed the repeated acid saline-induced generalized pain (AcGP) model, as an experimental mouse model of FM. In this model, the unilateral repeated acid injection into gastrocnemius muscle induced transient and long-lasting mechanical hypersensitivity. We focused on the spleen, a secondary lymphoid organ, and found that the intravenous treatments of splenocytes derived from AcGP mice caused mechanical hypersensitivity in naїve mice. Since the spleen is directly innervated by sympathetic nerve, we examined whether adrenergic receptors are necessary for pain development or maintenance. The administration of butoxamine, a selective β2-blocker, prevented the development but did not reverse the maintenance of pain-like behavior in AcGP mice. Furthermore, β2-blockade in donor AcGP mice eliminated pain reproduction in recipient mice injected with AcGP splenocytes. We currently employed another model of FM, the intermittent psychological stress-induced generalized pain (IPGP) model and found that as in AcGP model, the sympathetic nervous system and the spleen play important roles. These results suggest that sympathetic β2 signaling is enhanced by physical/psychological stress, and that immune system cells in the spleen activated in response play an important role in the formation and maintenance of chronic pain.

Chemodiversity and Antinociceptive Activity of Amorpha fruticosa L. Essential Oil

An essential oil dominated by germacrene D (19.3% by GC) was isolated from the fresh fruit of Amorpha fruticosa L. (Fabaceae). Agglomerative clustering and k-means clustering were employed to compare the composition of the oil with the existing literature data, suggesting that the A. fruticosa used in this study represents a new chemotype. The essential oil was evaluated for its antinociceptive activity using the acetic acid-induced writhing test in rats at doses of 400, 200, and 100 mg/kg. All tested doses reduced the number of writhes induced by the intraperitoneal injection of acetic acid. The 400 mg/kg dose of the oil demonstrated a 54.4% inhibition, which was statistically different from the positive control, aspirin, which showed 90.2% inhibition at a dose of 200 mg/kg. Since the injection of acetic acid produces the release of prostaglandins, such as PGE2α and PGF2α, as well as sympathetic nervous system mediators in peritoneal fluids, the results suggest that the inhibition of prostaglandin release might represent one of the possible mechanisms of action exerted by the oil.

Efficacy of Subdermal Poly-d,l-Lactic Acid Injections for the Treatment of Melasma.

Melasma is a chronic, recurrent skin disorder with limited long-term treatment success using conventional therapies like hydroquinone and laser treatments, which primarily target epidermal components while leaving dermal aspects untreated. To evaluate the efficacy and safety of poly-d,l lactic acid (PDLLA) subdermal injections for treating moderate melasma. Three female patients (age range: 45-59 years) with Fitzpatrick skin types III and IV received three PDLLA injection sessions at 3-week intervals. Treatment outcomes were assessed using the Melasma Area and Severity Index (MASI) and patient satisfaction scores at 12-week follow-up. All patients showed significant MASI score improvements (reduction range: 3.60-6.30 points). Patient satisfaction ratings ranged from 3 to 4 out of 4. Temporary side effects included mild edema and bruising, resolving within 72 h. PDLLA subdermal injections showed promising results in melasma treatment, potentially due to its biostimulatory effects on collagen production and dermal remodeling. Further research, including histopathological analysis, is needed to confirm long-term efficacy and safety, and understand underlying mechanisms.

Controls of low injectivity caused by interaction of reservoir and clogging processes in a sedimentary geothermal aquifer (Mezőberény, Hungary)

Low injectivity is often experienced in geothermal doublets installed in sandstone reservoirs. This even led to a shutdown of the Mezőberény (Hungary) geothermal site. An on-site campaign was carried out in January 2021 to prepare a stimulation aiming to enhance the transmissivity of the sedimentary reservoir and the near-wellbore zone of this site. Previous studies have concluded that insufficient injectivity may be linked to a high skin effect in the near well-bore zone and pore clogging in combination with the low net sandstone content of the fluvio-deltaic reservoir. A chemical soft stimulation based on the injection of hydrochloric acid (HCl) was successfully used to unclog and recover the well injectivity. Despite such empirical evidence, the geochemical mechanisms leading to both, detrimental formation of clogging and the HCl-driven transmissivity restoration, have not yet been elucidated. This work presents the results of a novel analysis aiming at (a) predicting the dominant type of clogging forming in the near-well bore zone; (b) quantifying the drop in hydraulic conductivity as clogging occurs; and (c) supporting the optimization of the HCl dosage during the chemical soft stimulation. The study is supported by new experimental datasets never presented before from the Mezőberény site and a geochemical model set-up simulating the main mechanisms involved in the clogging and unclogging processes. It is concluded that the biofilm formation was the dominant, while the precipitation of calcite and amorphous ferrihydrite—later reduced to magnetite by microbes—was the secondary clogging mechanism: In the long-term (yearly scale) simulating the hydraulic conductivity showed a decline with forming scales; therefore, biofilm was presumably responsible for the experienced rapid (1 month) clogging. When modelling the chemical stimulation, the estimated amount of precipitated minerals was dissolved already with 2.5 mol of HCl per liter of water (~ 10 m/m%). Therefore, the 20 m/m% of HCl chosen during the field campaign might had a beneficial effect dissolving the potentially higher amount of scaling and/or the carbonate minerals of the matrix near the wellbore. Overall, it is concluded that the chemical and the microbial analyses together with the geochemical model were critical to tailor the remediation attempts and to propose further development or reconstruction of the surface system before going into operation to prevent recurrent impairments. Our findings highlight the importance of interactions of various clogging mechanisms with each other as well as with the reservoir processes and provide approaches to tackle the issue of injectivity drop by characterizing and quantifying their effects.

Is human albumin injection the best choice to treat hypoalbuminemia?

Currently, there is an irrational use of drugs in the treatment of hypoproteinemia. This study evaluates the value of 12 injections to provide a basis for drug selection for the treatment of hypoproteinemia. The content of the first restrictive amino acid, the comprehensive quality of the total essential amino acid, and the closeness to the whole egg protein or FAO/WHO model were evaluated to compare their value in the amino acid synthesis of human serum albumin. A comparison was made between the matching degree of HA and therapeutic amino acids with the human plasma amino acid profile. Furthermore, the value and safety of synthetic human serum albumin were compared. The lowest synthetic value of human serum albumin was 18AA-V, and the highest was 18AA-II. The CS values of HA, 18AA-V, 18AA-II, 18AA-Ip, 18AA-IIp, and 19AA-Ip were 0.18, 0.58, 0.78, 0.55, 0.54, and 0.59, respectively. The similarity to egg protein was 0.81, 0.92, 0.94, 1.00, 1.18, and 1.18, respectively. The proximity values to FAO/WHO standards were 0.81, 0.85, 0.90, 1.36, 1.54, and 1.54, respectively. The changes in 3AA and the amino acid profile were matched when liver function was abnormal. When the renal function was abnormal, 9AA was matched. During trauma, 18AA-VII was matched. The amino acid profile of HA did not correspond. Patients with normal liver and kidney function should choose compound balanced amino acid injection, while patients with abnormalities should choose 3AA. Patients with renal dysfunction should choose 9AA. Trauma patients should choose 18AA-VII.

Mentum, Mouth Floor, and Tongue Necrosis Following Hyaluronic Acid Injection for Chin Augmentation.

Hyaluronic acid injection for chin augmentation presents a minimally invasive technique that has garnered increasing popularity. Although the chin region is generally regarded as relatively safe with a lower likelihood of ischemic complications, it is imperative to recognize that severe adverse events can still arise due to variations in vascular or bone structures. This article documents a rare case of necrosis affecting the chin skin, floor of the mouth, and tongue after hyaluronic acid injection for chin augmentation, which was successfully managed with reconstituted hyaluronidase injection.

Research on improving permeability of granite reservoir: Polyhydrogen chelating acid acidizing

ABSTRACT Acidization is a highly effective technique for increasing oil well productivity. Therefore, it is critical to study effective stimulation of low-permeability granite reservoirs using acidification. Using the granite reservoir of Baobab oilfield in Chad as the research object, the properties of reservoir rocks were tested using XRD and rock thin section analysis. Dissolution experiments, core flooding experiments, and CT scanning were used to investigate the increased permeability and changes in the pore structure of the cores during acidification. The minerals in the rocks are primarily plagioclase, kalifeldspar, and quartz, with trace amounts of calcite, chlorite, magnetite, illite, biotite, hornblende, sphene, and sericite. At 80°C and 10 mL of main acid injection, polyhydrogen chelating acid (6% HCl + 5% SA601 + 7% SA701) improved granite permeability more than mud acid (8% HCl + 2% HF) and fluoroboric acid (8% HCl + 8% HBF4), with permeability increase of 3.68. The CT three-dimensional images of the rocks show that both the cores acidified with polyhydrogen chelating acid and mud acid formed continuous acid-etched wormholes, while the cores acidified with fluoroboric acid did not. Compared to the mud acid, the core acidified by the polyhydrogen chelating acid produced more visible acid-etched wormholes. Meanwhile, the polyhydrogen chelating acid was more effective at improving the pore throats and expanding the flow channels in the core. This study serves as a reference for the application of acidification and increasing production in low-permeability granite reservoirs.

Can molecular oscillation technology be used as an alternative to acid injection to prevent the clogging of emitters in drip irrigation systems?

AbstractIn this study, molecular oscillation technology (Merus ring), developed to prevent calcification, biofilm formation and corrosion in piping in industrial water treatment systems, was compared as an alternative to acid injection to prevent the clogging of emitters by chemicals, and fresh and saline water were compared on the basis of system performance parameters (coefficient of variation, distribution uniformity, Christiansen uniformity, emission uniformity) and economic aspects. The results revealed that both acid injection and the Merus ring in saline and fresh water resulted in significantly higher average emitter flow rates than the control treatment. Both the Merus ring and the acid injection achieved higher performance parameters with fresh water than with saline water. Acid injection was found to be more effective in preventing clogging of the emitters than the Merus ring when saline water was used. Compared with fresh water, saline water increases emitter clogging. The economic analysis revealed that the Merus ring was more economical ($204.3 ha−1) than acid injection with both fresh water ($318.2 ha−1) and saline water ($578.6 ha−1). Therefore, the Merus ring could be a reliable and environmentally friendly alternative to acid injection to prevent the clogging of emitters in drip systems when saline water is used.

Injection treatments for trapeziometacarpal joint arthritis: a network meta-analysis of randomized studies.

In this network meta-analysis of 13 randomized studies comparing injection treatments for trapeziometacarpal joint arthritis, we found that the only treatments statistically and clinically superior to placebo were corticosteroid and hyaluronic acid injections for mid-term pain.

DNA methylation profiles of transgenerational rat hyperactivity primed by silver nanoparticles: Comparison with valproate model rats of autism

There is an increasing body of evidence suggesting that a single exposure to certain chemicals can have transgenerational effects, with the underlying mechanism believed to be epigenetic. However, it remains largely unknown whether psychiatric conditions like ADHD or autism, induced by environmental chemicals, can be inherited across generations. Pregnant rats were purchased from a commercial breeder. On the 7th day of gestation (E7), they were divided into two groups: one group was orally exposed to silver nanoparticles (AgNP; 4 mg/kg), while the control group received vehicle alone. The subsequent generation (F1) underwent spontaneous motor activity (SMA) measurements at 8–11 weeks of age. For breeding at 26 weeks of age, rats with higher SMA were selected from hyperactive litters, while untreated rats were randomly selected. This process was continued for four generations in both groups. The AgNP-primed rats at 4th generation displayed significantly higher SMA, 1.8 times greater than that of untreated rats. Intraperitoneal injection of valproic acid (150 mg/kg), an epigenetic modifier to 5-day-old rats causes adult hyperactivity (1.4-fold), suggesting that epigenetic modification contributes to rat hyperactivity. Global DNA methylation profiles in the mesencephalon were positively correlated in both groups of hyperactive rats. Furthermore, there were 7–8 common genes showing both hypermethylation and hypomethylation, which are involved in neuronal development, neuronal function, transcriptional activity, DNA binding activity, cell differentiation, ubiquitination processes, or histone modification, including Pax 6 and Mecp 2. Thus, it is most likely that rats retain hyperactivity through mesencephalic DNA methylation status across transgeneration.

An out-of-season cold: filler-induced vascular occlusion of the columellar artery causing necrosis of the cartilaginous anterior nasal septum case report and a literature review

In their recent publication, George Kroumpouzos et al. provided a comprehensive update on the complications caused by filler procedures in the lips and perioral area. These injections have been associated with various arterial accidents and an incidence of vision loss mainly when such procedures are performed at the level of the central facial massif. Among the various cases of vascular involvement reported in the literature, we have found what we believe to be a rare occurrence of columellar artery occlusion with a noteworthy discrepancy between the expected symptoms and the actual described vascular effects. The necrosis of the columellar artery following hyaluronic acid injections showed some peculiarities: its frequency is unknown, and recognition can be troublesome due to the presentation of subtle symptoms. We want to exemplify these characteristics in this case, which, fortunately, optimally resolved itself, underscoring its unique nature.

Three-dimensional Stereoscopic Evaluation and Quantitative Gray Scale Assessment of the Nasolabial Fold and Its Application in Injection Therapy

With the growing demand for aesthetic enhancements, particularly filler injections for nasolabial folds (NLFs), there is a need for more objective and quantitative assessment methods beyond the traditional subjective scales like the Wrinkle Severity Rating Scale (WSRS). This study introduces a novel evaluation method combining 3-dimensional (3D) imaging and grayscale analysis to objectively assess NLF severity and treatment outcomes. A total of 100 healthy participants (86 females, 14 males; mean age 41.85±11.53 years) were assessed using WSRS scores and quantitative 3D measurements. The results demonstrated a significant positive correlation between WSRS scores and gray values (r=0.985, P &lt; 0.05). Significant differences in gray values and 3D linear measurements were observed across WSRS score groups, with high intra-rater reliability confirmed by intraclass correlation coefficients. In addition, 54 patients (mean age 42.9±8.9 y) with moderate to severe NLFs received hyaluronic acid injections. One month post-treatment, WSRS scores significantly decreased (from 3.74±0.45 to 1.63±0.59, P &lt; 0.05), and significant improvements were observed in several 3D measurements. The average gray value also decreased significantly (P &lt; 0.05), indicating a reduction in wrinkle depth. These findings were supported by representative photos showing patient satisfaction and no severe complications. This study validates the use of 3D imaging and grayscale analysis as reliable tools for objectively assessing NLF severity and monitoring treatment efficacy, potentially improving the precision and reliability of aesthetic evaluations in clinical practice.

Phosphatidic acid is involved in regulation of autophagy in neurons in vitro and in vivo.

Major depressive disorder (MDD) is a common and severe psychiatric disease, which does not only lead to variety of neuropsychiatric symptoms, but unfortunately in a relatively large proportion of cases also to suicide. The pathogenesis of MDD still requires definition. We have previously shown that ceramide is increased in the blood plasma of patients with MDD. In mouse models of MDD, which are induced by treatment with corticosterone or application of chronic unpredictable stress, increased blood plasma ceramide also increased and caused an inhibition of phospholipase D in endothelial cells of the hippocampus and reduced phosphatidic acid levels in the hippocampus. Here, we demonstrated that corticosterone treatment of PC12 cells resulted in reduced cellular autophagy, which is corrected by treatment with phosphatidic acid. In vivo, treatment of mice with corticosterone or chronic unpredictable stress also reduced autophagy in hippocampus neurons. Autophagy was normalized upon i.v. injection of phosphatidic acid in these mouse models of MDD. In an attempt to identify targets of phosphatidic acid in neurons, we demonstrated that corticosterone reduced levels of the ganglioside GM1 in PC-12 cells and the hippocampus of mice, which were normalized by treatment of cells or i.v. injection of mice with phosphatidic acid. GM1 application also normalized autophagy in cultured neurons. Phosphatidic acid and GM1 corrected stress-induced alterations in behavior, i.e., mainly anxiety and anhedonia, in experimental MDD in mice. Our data suggest that phosphatidic acid may regulate via GM1 autophagy in neurons.

The Influence of Sulfuric Acid Injection on Deposit Formation in a Waste-to-Energy Plant

The Influence of Sulfuric Acid Injection on Deposit Formation in a Waste-to-Energy Plant

Comparison of Ultrasound Guided Intra- Articular Injection of Platelet Rich Plasma Versus High Molecular Weight Hyaluronic Acid in Improving Cartilage Thickness and Physical Functions in Patients with Primary Osteoarthritis of Knee: A Randomized Controlled Trial

Comparison of Ultrasound Guided Intra- Articular Injection of Platelet Rich Plasma Versus High Molecular Weight Hyaluronic Acid in Improving Cartilage Thickness and Physical Functions in Patients with Primary Osteoarthritis of Knee: A Randomized Controlled Trial

Efficacy of intra-articular injection of combined platelet-rich-plasma (PRP) and hyaluronic acid (HA) in knee degenerative joint disease: a prospective, randomized, double-blind clinical trial.

Intra-articular injections of hyaluronic acid (HA) and platelet-rich plasma (PRP) represent promising options in the conservative treatment of early stages of knee osteoarthritis (OA). Although the combined use of these two compounds seems to have a synergistic effect in pre-clinical studies, few clinical trials compared the association of PRP and HA with PRP and HA alone. The aim of study is toevaluate the efficacy of combined intra-articular injections of PRP and HA in the treatment of mild to moderate knee OA, compared to PRP and HA alone. One hundred and seventy-four patients were prospectively enrolled and randomized to undergo three intra-articular injections of either HA or PRP or the combination of PRP and HA with a two-week interval period. Patients were evaluated before treatment and after 3, 6, and 12months using Western Ontario and McMaster Universities Osteoarthritis (WOMAC) score, Knee Injury and Osteoarthritis Outcome Score, International Knee Documentation Committee subjective score, Visual Analogue Scale and TEGNER Activity Scores. All the treatments proved to be effective in reducing pain and improving joint function. The analysis of covariance did not show statistically significant differences among the three groups for any of the investigated outcome parameters after 6 and 12months (WOMAC: p = 0.45 and p = 0.64, respectively). No significant differences were found in terms of adverse events (p = 0.49) and painkiller use (p = 0.28 and p = 0.56, respectively) among the three groups. This multicenter, prospective, randomized, double-blind controlled trial revealed that intra-articular injections of PRP + HA, PRP, or HA represent a safe and viable treatment with comparable efficacy in terms of pain relief and functional outcomes in mild to moderate knee OA.

Ultrasound-guided infiltration with hyaluronic acid compared with corticosteroid for the treatment of Morton's neuroma.

A local injection may be used as an early option in the treatment of Morton's neuroma, and can be performed using various medications. The aim of this study was to compare the effects of injections of hyaluronic acid compared with corticosteroid in the treatment of this condition. A total of 91 patients were assessed for this trial, of whom 45 were subsequently included and randomized into two groups. One patient was lost to follow-up, leaving 22 patients (24 feet) in each group. The patients in the hyaluronic acid group were treated with three ultrasound-guided injections (one per week) of hyaluronic acid (Osteonil Plus). Those in the corticosteroid group were treated with three ultrasound-guided injections (also one per week) of triamcinolone (Triancil). The patients were evaluated before treatment and at one, three, six, and 12 months after treatment. The primary outcome measure was the visual analogue scale for pain (VAS). Secondary outcome measures included the American Orthopaedic Foot and Ankle Society (AOFAS) score, and complications. Both groups showed significant improvement in VAS and AOFAS scores (p < 0.05) after 12 months. The corticosteroid group had a significantly greater reduction in VAS and increase in AOFAS scores compared with the hyaluronic acid group, at one, three, and six months, but with no significant difference at 12 months. There were no complications in the hyaluronic acid group. There were minor local complications in six patients (six feet) (25.0%) in the corticosteroid group, all with discolouration of the skin at the site of the injection. These minor complications might have been due to the three weekly injections of a relatively high dose of corticosteroid. No patient subsequently underwent excision of the neuroma. An ultrasound-guided corticosteroid injection showed statistically significantly better functional and pain outcomes than an ultrasound-guided injection of hyaluronic acid for the treatment of a Morton's neuroma at many timepoints. Thus, a corticosteroid injection should be regarded as a primary option in the treatment of these patients, and the only indication for an injection of hyaluronic acid might be in patients in whom corticosteroid is contraindicated.

Sodium valproate ablates ferroptosis in kainic acid-induced epileptic seizure via suppressing lysyl oxidase.

The objective of this study is to explore whether sodium valproate (VPA) alleviates epileptic seizures via suppressing lysyl oxidase (Lox)-mediated ferroptosis. Epileptic seizure mouse model was prepared via intrahippocampal injection of kainic acid (250 ng/μl). After treatment with kainic acid, VPA was injected intraperitoneally by the dose of 250 mg/kg twice daily for 4 days. Ferroptosis-associated indices including lipid peroxides (LPO) level and Ptgs2 mRNA in hippocampal tissue samples were detected. Additionally, effects of VPA on Lox mRNA and enzymatic activity were assessed by quantitative real-time PCR and a commercial kit, respectively. Neuronal survival was assessed by Nissl staining. In kainic acid-induced epileptic seizure mouse model, VPA significantly suppressed LPO level and Ptgs2 mRNA and the suppression of ferroptosis was positively correlated with its anti-seizure effect. Lox mRNA and enzymatic activity were also found to decrease in hippocampus of epileptic seizure mice after VPA treatment. Furthermore, overexpression of Lox via adeno-associated virus infection remarkably abrogated the inhibitory effect of VPA on ferroptosis and neuronal impairment together with its anti-seizure effect. VPA suppresses Lox-mediated ferroptosis process, which can provide the explanation for its anti-seizure property.