Initial Condensation Research Articles

Phase Diagram of Initial Condensation for Two-Layer Neural Networks

Phase Diagram of Initial Condensation for Two-Layer Neural Networks

Experimental and theoretical study of single bubble formation at the nozzle in a liquid oxygen crossflow

In this paper, the condensation bubbles generated by the mixing and condensation of gaseous and liquid oxygen crossflow experimentally and theoretically. The mixing and condensation experiment of the bubbles was carried out, employing gaseous and liquid oxygen as working fluids in vertical tubes. Three successive flow patterns were observed: initial gas plume, condensation oscillation, and separation condensation. The corresponding characteristics include gas mass aggregation, bubble surface smoothness and wrinkling alternately, and bubble disappearance after separation and condensation. Then, a Nusselt number (Nu) heat transfer correlation formula is fitted with a corresponding fitting error in the range of −31% ∼ 35%. In addition, bubble force balance and conservation equations were employed to establish a mixing condensation bubble model suitable for gaseous and liquid oxygen in vertical tubes, and the error between theoretical and experimental results was in the range of −25% ∼ 20%. Furthermore, the effects of working conditions and forces on bubble condensation length has been analyzed. The results showed that increasing the liquid oxygen flow rate, reducing the gas oxygen volume flow rate, or appropriately reducing the gas inlet diameter led to reducing the bubble condensation length. It was shown that the bubble drag and shear lift forces affect the condensation length mostly.

Green vanillin coupled with P/N/Si flame retardant for poly (L-lactic acid)

To better solve the flammability of poly (l-lactic acid) (PLLA) and meet environmental concerns, developing bio-derived flame retardants for PLLA is an alternative method. A green flame retardant (VKD) was synthesized in the work. Vanillin was modified using 3-amino-propyl-triethoxysilane (KH550) prior to coupling with 9, 10-dihydro-9-oxa-10-phosphafi-10-oxide (DOPO). As the blend ratio of VKD to PLLA was 20 wt%, the limiting oxygen index (LOI) and UL-94 were 25.5% and V-0 rating, respectively; and the residual carbon rate reached 9.14% at 600 °C, which was 3.3 times higher than that of PLLA. In the cone calorimetry test, the peak heat release rate (pHRR) and total heat released (THR) reduced by 9.9% and 20.5%, respectively, and total smoke production (TSP) decreased by 26.8%, indicating that VKD/PLLA had good flame retardancy and smoke suppression properties. It was mainly attributed to initial condensation phase obstacle and following combination of gaseous phase quenching and carbon/silicon composite barrier. The VKD/PLLA maintained balanceable mechanical properties and good degradation. Then green VKD would broaden PLLA in the application of engineering such as electronic products and automotive interior materials.

Synthesis of a Novel Tetracyclic Isoxazole by Intramolecular Nitrile Oxide Cycloaddition

Intramolecular cycloadditions have the great advantage of forming two rings simultaneously. We report the use of intramolecular [3 + 2] cycloaddition of the nitrile oxide derived from an N-propargylbenzimidazole oxime in the synthesis of a hitherto unreported tetracyclic isoxazole-containing ring system bearing “6-5-5-5”-membered ring fusions. The initial condensation was achieved through reaction of o-phenylenediamine with ethyl diethoxyacetate, followed by alkylation with propargyl bromide, deprotection of the acetal to the aldehyde, formation of an aldoxime, and intramolecular nitrile oxide cycloaddition (INOC). Characterization of the aldoxime and tetracyclic isoxazole is included herein.

A Tandem Aldol Condensation/Diels‐Alder Sequence of Reactions for One‐Pot Synthesis of a New Series of Polysubstituted‐Decalines

AbstractA tandem aldol condensation/Diels‐Alder sequence of reactions was designed leading to the synthesis of a new series of derivatives of polysubstituted‐decalines. Both steps of the process took place in the same pot and in the presence of H2O/PEG as the medium and diethylamine as the catalyst of the process. The required dienes were formed in situ and reacted with the dienophile (acrylonitrile) to produce high yields of the final decaline derivatives. Mechanistic studies suggested that the process goes through an initial aldol condensation reaction followed by a [4+2] cycloaddition step and then an olefinic bond rearrangement to give the final adducts. To support the proposed mechanism, the intermediate dienes were synthesized separately as well and their stepwise reactions with the dienophile led to the same products. Ten derivatives of the target structure were prepared and then characterized by various spectroscopic methods and X‐ray crystallography analysis. The procedure was also developed to engage some other dienophiles successfully.

Mechanistic Insight into Acceptorless Dehydrogenation of Methanol to Syngas Catalyzed by MACHO-Type Ruthenium and Manganese Complexes: A DFT Study.

The acceptorless dehydrogenation of methanol to produce carbon monoxide (CO) and dihydrogen (H2) mediated by MACHO-type 1-Ru and 1-Mn complexes was theoretically investigated via density functional theory calculations. The 1-Ru-catalyzed process involves the formation of active species 4-Ru through a methanol-bridged H2 release pathway. Methanol dehydrogenation by 4-Ru yields formaldehyde and 1-Ru, followed by H2 release to regenerate 4-Ru (rate-determining step, ΔG‡ = 32.5 kcal/mol). Formaldehyde further reacts with methanol via nucleophilic attack of the MeO- ligand in the Ru complex (ΔG‡ = 9.6 kcal/mol), which is more favorable than the traditional methanol-to-formaldehyde nucleophilic attack (ΔG‡ = 33.8 kcal/mol) due to the higher nucleophilicity of MeO-. CO is ultimately produced through the methyl formate decarbonylation reaction. Accelerated H2 release in the early reaction stage compared to CO results from the initial methanol dehydrogenation and condensation of formaldehyde with methanol. In contrast, CO generation occurs later via methyl formate decarbonylation. The 1-Mn-catalyzed reaction has reduced efficiency compared to 1-Ru for the higher Gibbs energy barrier (ΔG‡ = 34.1 kcal/mol) of the rate-determining step. Excess NaOtBu promotes the reaction of CO and methanol, forming methyl formate, significantly reducing the CO/H2 ratio as the catalyst amount decreases. These findings deepen our understanding of the methanol-to-syngas transformation and can drive progress in this field.

Wastewater purification from Rhodamine B and Gemifeloxacine (GEM) by GOPF nanocomposite: A novel MD simulation for experimental contaminants removing.

In this study, the synthesized nanocomposite was evaluated novel graphene oxide/pectin/ferrite (GOPF) adsorbent to the adsorption of Rhodamine B (RhB) and Gemifloxacin (GEM) from wastewater. Theoretical studies were carried out using quantum simulation via the Forcite module in Material Studio 2017. The simulation results demonstrated RhB and GEM adsorption over other dyes and drugs. The synthesized nanocomposite was identified by BET, TGA, FT-IR, FE-SEM, XRD, VSM, and EDS. The nanocomposite's ability to effectively take RhB and GEM from an aqueous solution was checked by performing a series of experiments based on the effect of adsorbent dose, initial condensation, contact time, pH, and temperature. The nanocomposite kinetics follow a PSO. The Freundlich isotherm model were applied for maximum adsorption capacity of GEM (124.37 mg/g) and RhB (86.60 mg/g) on GOPF nanocomposite. According to the antibacterial activity test, the synthesized nanocomposite can kill bacteria 5 mm in diameter. Also, the anti-cancer test of nanocomposite was done with 75% viability in high concentrations of nanocomposite. Thus, GOPF application results not only are suitable for dyes but only satisfying for drugs.

Conundrum of dehydroascorbic acid and homocysteine thiolactone reaction products: Structural characterization and effect on peptide and protein N-homocysteinylation.

An excessive blood level of homocysteine (HcySH) is associated with numerous cardiovascular and neurodegenerative disease conditions. It has been suggested that direct S-homocysteinylation, of proteins by HcySH, or N-homosteinylation by homocysteine thiolactone (HTL) could play a causative role in these maladies. In contrast, ascorbic acid (AA) plays a significant role in oxidative stress prevention. AA is oxidized to dehydroascorbic acid (DHA) and if not rapidly reduced back to AA may degrade to reactive carbonyl products. In the present work, DHA is shown to react with HTL to produce a spiro bicyclic ring containing a six-membered thiazinane-carboxylic acid moiety. This reaction product is likely formed by initial imine condensation and subsequent hemiaminal product followed by HTL ring opening and intramolecular nucleophilic attack of the resulting thiol anion to form the spiro product. The reaction product was determined to have an accurate mass of 291.0414 and a molecular composition C10H13NO7S containing five double bond equivalents. We structurally characterized the reaction product using a combination of accurate mass tandem mass spectrometry, 1D and 2D-nuclear magnetic resonance. We also demonstrated that formation of the reaction product prevented peptide and protein N-homocysteinylation by HTL using a model peptide and α-lactalbumin. Furthermore, the reaction product is formed in Jurkat cells when exposed to HTL and DHA.

Spatial variation of moisture content along the axial distance of corn at different superheated steam drying temperatures and various instantaneous times

AbstractSuperheated steam acts as both a heat source and a drying medium. The study sought to predict the evolution of the moisture transport behaviour of the corn kernel at various axial distances at varying instantaneous time. The equations describing the drying phases were solved using numerical solutions with the Eulerian technique in ANSYS software. Cone geometry was used to simulate the corn kernel with initial moisture content at 20% w.b. Steam conditions were similar to what is encountered in industry, with temperatures ranging (from 120–200°C) at 1.5 m s−1 velocity. ANOVA was used to determine if there was difference between the conditions. The temporal change in moisture from the apex to the periphery varied at superheated steam temperatures 120, 160 and 200°C. At 10, 100 and 200 s the drying rate and effective moisture diffusivity of corn kernel from the centre towards the periphery differed. Post‐hoc analysis with Bonferroni adjustment revealed that moisture content (w.b.%) differed between 10 and 100 s, 10 and 200 s and 100 and 200 s. The mean difference was attributed to the drying being in the falling drying phase at 200 s and initial condensation at 10 s. Thus, at high superheated steam temperatures, dry zones can be seen as the axial distance from the apex increases toward the periphery.

Thermodynamics of mechanopeptide sidechains

Biological systems are often exposed to mechanical perturbations, which may modulate many biochemical processes. Ligand binding involves a wide range of structural changes in the receptor protein, from hinge movement of entire domains to minor sidechain rearrangements in the binding pocket residues. Hydrophobic ligand binding to protein alters the system’s vibrational free energy, allowing different conformational states of allosteric proteins. Excess hydrophobicity in protein–ligand binding generates mechanical force along the peptide backbone through the hydrophobic effect. We describe mechanically strained peptide structures involved in protein aggregation to determine the transition between the initial condensation of hydrophobic polypeptide chains into ordered fibrillar structures. This transition is due to the excess attractive hydrophobic force by ligand binding within proteins into fibrillar assemblies. The process of fibrillar formation has a mechanosensitive nature, which significantly influences the pathogenesis of several neurodegenerative diseases.

Lack of skeletal muscle contraction disrupts fibrous tissue morphogenesis in the developing murine knee.

Externally applied forces, such as those generated through skeletal muscle contraction, are important to embryonic joint formation, and their loss can result in gross morphologic defects including joint fusion. While the absence of muscle contraction in the developing chick embryo leads to dissociation of dense connective tissue structures of the knee and ultimately joint fusion, the central knee joint cavitates whereas the patellofemoral joint does not in murine models lacking skeletal muscle contraction, suggesting a milder phenotype. These differential results suggest that muscle contraction may not have as prominent of a role in the growth and development of dense connective tissues of the knee. To explore this question, we investigated the formation of the menisci, tendon, and ligaments of the developing knee in two murine models that lack muscle contraction. We found that while the knee joint does cavitate, there were multiple abnormalities in the menisci, patellar tendon, and cruciate ligaments. The initial cellular condensation of the menisci was disrupted and dissociation was observed at later embryonic stages. The initial cell condensation of the tendon and ligaments were less affected than the meniscus, but these tissues contained cells with hyper-elongated nuclei and displayed diminished growth. Interestingly, lack of muscle contraction led to the formation of an ectopic ligamentous structure in the anterior region of the joint as well. These results indicate that muscle forces are essential for the continued growth and maturation of these structures during this embryonic period.

Research on non-equilibrium condensation of supercritical carbon dioxide in sCO2 power systems

Based on the consideration of sustainable development and energy efficient transformation, people propose using renewable energy to upgrade traditional power generation systems. The supercritical carbon dioxide (sCO2) Brayton cycle has a great utilization potentiality in thermodynamic systems, such as solar thermal power generation systems. When the CO2 fluid flows into the compressors of sCO2 power systems, there is a risk of non-equilibrium condensation due to local expansion and acceleration, which seriously threatens the stability of the whole system. The convergent-divergent nozzle is usually used to study non-equilibrium condensation. In this paper, the wet steam model is used to simulate the non-equilibrium condensation of the CO2 fluid near its critical region in a convergent-divergent nozzle. The phase change model and the thermophysical equation of state are verified by experimental data. The non-equilibrium condensation process is analyzed, and the droplet nucleation process and droplet growth process are described. Non-equilibrium condensation releases the latent heat and forms a condensation shock wave. The influences of the shock wave effect on temperature, pressure, and other parameters are discussed. The initial condensation position and critical subcooled temperature under different inlet conditions are analyzed.

Computational modeling of spatial variation in moisture content and temperature distribution in corn at different superheated steam temperatures

The Superheated steam has been shown to be more effective than hot air for drying corn. Modeling studies have been carried out in fluidized bed dryers to determine the moisture and heat transfer characteristics of corn, but there are no modeling studies for a single corn kernel. Knowledge of heat and mass transfer characteristics in a single kernel would produce a better understanding of steam characteristics needed for specific drying rates. The study sought to determine the influence of steam temperature and velocity on the drying rate of a corn kernel. It used computational fluid dynamics (CFD) to model the transfer of heat and moisture between the corn kernel and superheated steam. The simulation used cone geometry to represent the corn kernel. The kernel had characteristic dimensions and 20% initial moisture content. The condition of the steam, which was typical of that found in industry, had temperatures of 120–200°C and velocities of 0.5–1.5 m/s. There was a decrease in the duration of the initial condensation phase as the temperature of superheated steam increased from 120°C to 200°C. The highest steam temperature and velocity resulted in the shortest duration for steam condensation. Contrary to what has been reported, there was no moisture loss from the corn kernel when exposed to superheated steam at 120°C, which may be due to how heat was retained in the drying chamber. Thus, the processes of tapping steam and retaining heat are important elements in the operation of industrial steam driers.

Optimization of gas recycling technique in development of gas-condensate fields

Purpose. The research purpose is to increase the efficiency of development of gas condensate fields with a high condensate yield in the reservoir gas and to develop optimal ways of increasing their hydrocarbon recovery. Methods. The effectiveness of the implementation of reservoir pressure maintenance technologies using dry gas for the development of gas condensate fields with a high condensate yield in the reservoir gas is studied on the basis of a heterogeneous 3D model using the Schlumberger Eclipse and Petrel software packages. The technological indicators of the development of gas-condensate reservoir are studied for different pressure values at the beginning of the dry gas injection. Calculations were made for pressures at the beginning of injection at the level of: 1.0 Рinit; 0.8 Рinit; 0.6 Рinit; 0.4 Рinit; 0.2 Рinit. Findings. It has been determined that when the dry gas is injected into a gas-condensate reservoir, reservoir pressure is maintained at a significantly higher level than it is in the case of depletion. This ensures stable operation of production wells over a longer period of the reservoir development. According to the research results, it should be noted that in the case of implementation of the reservoir pressure maintenance technology, a part of the precipitated condensate is transferred to the gas phase, which makes it necessary to extract it together with the reservoir gas. Based on the modeling results, the ultimate condensate recovery factor have been calculated. The calculation results indicate that in the case of the cycling process implementation, the ultimate condensate recovery factor of the gas-condensate reservoir increases by 7.26% compared to depletion development. Originality. Based on the calculation data analysis, the optimal pressure value at the beginning of dry gas injection into a gas-condensate reservoir has been determined, which is 0.842 Рinit. Practical implications. The use of the conducted research results will optimize the development system of gas-condensate fields with high initial condensate yield in the reservoir gas and increase the efficiency of development the explored hydrocarbon reserves in the conditions of a significant shortage of hydrocarbon raw materials in Ukraine. The conducted research results indicate the high technological efficiency of the reservoir pressure maintenance technology using dry gas.

Liquid products of meat and bone meal pyrolysis: comprehensive assessment by chromatographic methods

Dorogov’s antiseptic stimulators (fractions 2 and 3) are products of meat and bone meal pyrolysis that are used to treat farm animals. However, there is a lack of detailed information about their chemical composition. We aimed to study individual compositions of organic substances in the water- and oil-soluble condensates of these preparations.
 Dorogov’s antiseptic stimulators ASD-2F and ASD-3F (Agrovetzashchita, Russia) were used as samples of the water- and oil-soluble condensates of meat and bone meal pyrolysis. Volatile substances were identified by gas chromatography and gas chromatography-mass spectrometry, while amino acids were determined by high-performance liquid chromatography.
 The initial water-soluble condensate contained ammonium salts, amides of carboxylic acids, N-heterocyclic compounds, hydantoins, amino acids, and dipeptides, with a total content of 8% of the condensate’s weight. Its dehydrated concentrate had almost no ammonium salts and amides of carboxylic acids, but its contents of hydantoins, amino acids, dipeptides, and lowvolatile nitrogen-containing heterocycles were 10–15 times as high as those in the initial condensate. The condensate contained 13 dipeptides and 19 amino acids with a total content of 2.5%. According to gas chromatography-mass spectrometry, the oilsoluble condensate contained over 30% of nitriles; 7–10% of higher and aromatic hydrocarbons, phenols, and amides (with esters); and 1–3% of N-heterocyclic compounds, naphthalenes, pyridines, and dipeptides. The nitrogen-containing heterocycles, as well as dipeptides, were similar to those in the water-soluble condensate.
 We identified 80% of individual organic substances in the water-soluble pyrolytic condensate. Together with its concentrate, they contained more than 220 organic substances divided into 10 main groups. The oil-soluble condensate consisted of over 350 individual organic compounds. The full composition of the preparations can be further identified by three-quadrupole liquid mass spectrometry.

Dimensional Reduction for Single-Molecule Imaging of DNA and Nucleosome Condensation by Polyamines, HP1α and Ki-67.

Macromolecules organize themselves into discrete membrane-less compartments. Mounting evidence has suggested that nucleosomes as well as DNA itself can undergo clustering or condensation to regulate genomic activity. Current in vitro condensation studies provide insight into the physical properties of condensates, such as surface tension and diffusion. However, methods that provide the resolution needed for complex kinetic studies of multicomponent condensation are desired. Here, we use a supported lipid bilayer platform in tandem with total internal reflection microscopy to observe the two-dimensional movement of DNA and nucleosomes at the single-molecule resolution. This dimensional reduction from three-dimensional studies allows us to observe the initial condensation events and dissolution of these early condensates in the presence of physiological condensing agents. Using polyamines, we observed that the initial condensation happens on a time scale of minutes while dissolution occurs within seconds upon charge inversion. Polyamine valency, DNA length, and GC content affect the threshold polyamine concentration for condensation. Protein-based nucleosome condensing agents, HP1α and Ki-67, have much lower threshold concentrations for condensation than charge-based condensing agents, with Ki-67 being the most effective, requiring as low as 100 pM for nucleosome condensation. In addition, we did not observe condensate dissolution even at the highest concentrations of HP1α and Ki-67 tested. We also introduce a two-color imaging scheme where nucleosomes of high density labeled in one color are used to demarcate condensate boundaries and identical nucleosomes of another color at low density can be tracked relative to the boundaries after Ki-67-mediated condensation. Our platform should enable the ultimate resolution of single molecules in condensation dynamics studies of chromatin components under defined physicochemical conditions.

A Method for the Dynamics of Vortices in a Bose-Einstein Condensate: Analytical Equations of the Trajectories of Phase Singularities

We present a method to study the dynamics of a quasi-two dimensional Bose-Einstein condensate which initially contains several vortices at arbitrary locations. The method allows one to find the analytical solution for the dynamics of the Bose-Einstein condensate in a homogeneous medium and in a parabolic trap, for the ideal non-interacting case. Secondly, the method allows one to obtain algebraic equations for the trajectories of the position of phase singularities present in the initial condensate along with time (the vortex lines). With these equations, one can predict quantities of interest, such as the time at which a vortex and an antivortex contained in the initial condensate will merge. For the homogeneous case, this method was introduced in the context of photonics. Here, we adapt it to the context of Bose-Einstein condensates, and we extend it to the trapped case for the first time. Also, we offer numerical simulations in the non-linear case, for repulsive and attractive interactions. We use a numerical split-step simulation of the non-linear Gross-Pitaevskii equation to determine how these trajectories and quantities of interest are changed by the interactions. We illustrate the method with several simple cases of interest, both in the homogeneous and parabolically trapped systems.

Suspended in sound: New constraints on isotopic fractionation of falling hydrometeors using acoustically levitated water droplets

AbstractThe isotopic composition of precipitation (δ18O, δ2H, and δ17O) is affected by evaporation and exchange as hydrometeors descend. These processes can significantly alter the isotopic ratio of precipitation relative to its initial condensation state in the cloud yet are exceedingly difficult to study in situ. The most widely utilized model for droplet‐atmosphere exchange was derived from controlled experiments where droplets were suspended by forced air in a narrow glass tube‐ a design that manipulated the structure of the boundary layer around the droplet. Here, we provide a novel experimental test of atmosphere‐hydrometeor isotopic exchange using the mechanism of acoustic levitation, where sound waves are projected vertically to levitate droplets in free‐flowing ambient air. We present results from a series of droplet levitation experiments where the droplets' surface temperatures were measured by a thermal camera and the background atmospheric isotope concentration was measured via cavity‐ringdown spectroscopy, providing a high degree of constraint on the fractionation conditions. We show that isotope enrichment of the suspended droplets met first order expectations based on existing models. However, to account for the slope of δ18O versus δ2H (i.e., the meteoric water line) and deuterium excess of the droplets as they evolved, we had to modify the existing model for droplet‐atmosphere exchange to account for the fact that some portion of the evaporative flux from the droplet remained present in the boundary layer around the droplet leading to an evolving feedback between droplet and the atmosphere‐that is, a quasi closed‐system effect. The isotopic enrichment of the boundary layer surrounding the droplet as a consequence of the closed‐system dynamics, drives more rapid δ18O isotope enrichment relative to δ2H of the droplet compared to what is predicted using an open system model. Although these were controlled experiments, they illustrate important dynamics regarding the isotopic signature of feedbacks between droplet evaporation and atmospheric humidity.

Synthesis and antiproliferative activity of new thiazole hybrids with [3.3.0]furofuranone or tetrahydrofuran scaffolds

New thiazole hybrids were synthesized and evaluated for their in vitro cytotoxicity against a panel of human malignant cell lines. The key steps in the synthesis of hybrids 3?7 involved the initial condensation of appropriate aldononitriles with cysteine ethyl ester hydrochloride, followed by subsequent treatment of resulting thiazolines with diazabicycloundecene to form the thiazole ring. Bioisosteres 8 and 14 have been prepared after the stereoselective addition of 2-(trimethylsilyl)thiazole to the hemiacetals obtained by periodate cleavage of terminal diol functionality in the suitably protected d-glucose derivatives. The obtained analogues showed various antiproliferative activities in the cultures of several tumour cell lines. Hybrid 6 was the most potent in HeLa cells, exhibiting more than 10 and 4 times stronger activity than both leads 1 and 2, respectively. The most active compound in Raji cells was hybrid 12, which was nearly 2-fold more potent than the clinical antitumour drug doxorubicin. All analogues were more potent in A549 cells with respect to lead 1, while compounds 6 and 7 were slightly more active than doxorubicin. Preliminary structure?activity relationship analysis revealed that the presence of a cinnamate group at the C-3 position in analogues of type 7 increases the activity of resulting molecular hybrids.

A step-by-step approach to creating and tuning PVT-models of reservoir hydrocarbon systems based on the state equation

The article is considering a new effective step-by-step approach to creating and tuning PVT-models of reservoir oil and reservoir gas condensate mixtures. The method is based on the reproducing of the results of field measurements and basic laboratory studies of representative samples in thermodynamic modeling using cubic three parameters equation of state. Tuning PVT-model is used for reliable modeling of PVT properties of reservoir fluids (reservoir oil and reservoir gas) in the design and monitoring of field development, calculation of multiphase flow in wells and field pipelines, as well as in basin modeling. Proposed approach makes possible to tune the PVT model with high accuracy of both reservoir oil and reservoir gas condensate system to experimental data using a step-by-step procedure, where at each step, by changing one of the parameters of the equation of state, one of the PVT properties of the hydrocarbon system is tuned. Algorithmization and automated application of this approach in specialized software products is possible. Proposed approach allows tune reservoir oil PVT-models on the main PVT-properties such as saturation pressure, FVF, STO density, gas-oil ratio of reservoir oil and dependencies of dynamic viscosity and compressibility on pressure at reservoir temperature as well as STO density. The tuned gas-condensate PVT-model precisely reproduces the key properties such as dew point pressure, initial condensate content in the reservoir gas, z-factor of the reservoir gas, gas-oil ratio, stable condensate density, drop down curve by the result of CVD-test. PVT-models created on the base of the proposed method, provide reliable information on the properties of a reservoir fluid in development of flow simulation both using a reservoir simulation compositional models and using pseudo models “black oil”. The method is illustrated by the example of creation of the adequate PVT-models of various regions of Russia reservoir oil and reservoir gas condensate mixtures.