Inhibitory Effect Of Salinity Research Articles

Heterogeneous saline water irrigation improves stomatal and nonstomatal restrictions to decrease yield reduction threshold in cherry tomato

Research on the response of tomato to homogeneous and heterogeneous salinity is essential for facility agriculture development in saline land. In this study, cherry tomatoes were irrigated with homogeneous or heterogeneous saline water in root zones by double root grafting. With irrigated fresh water (0/0) as a control, two homogeneous saline water irrigation treatments (S/S: 50/50, 100/100 mmol·L−1 NaCl) and two heterogeneous saline water irrigation treatments (0/S: 0/100, 0/200 mmol·L−1 NaCl) were used to analyze cherry tomato growth, photosynthesis, and osmoregulation under homogeneous and heterogeneous saline water irrigation. The results showed that the yields for the heterogeneous irrigation treatments with 0/100 and 0/200 mmol·L−1 NaCl were 1.2 and 3.1 times greater than those for the homogeneous treatments with 50/50 and 100/100 mmol·L−1 NaCl, respectively. The inhibitory effects of salinity on cherry tomato aboveground growth and stomatal restriction were alleviated by heterogeneous irrigation, which promoted photosynthesis and maintained a stable total root content compared with homogeneous salinity. In addition, the Na+ and Cl− contents in leaves under heterogeneous salinity were significantly reduced and high K+ and K+/Na+ levels as well as strong osmoregulation ability were maintained. In conclusion, the growth of and physiological damage to cherry tomato caused by salinity and the yield reduction threshold significantly improved under heterogeneous salinity. This could provide a theoretical basis for the adaptive cultivation of cherry tomato in saline land. We believe heterogeneous saline water irrigation, an irrigation technique for declining yield reduction thresholds in cherry tomato by improving stomatal and non-stomatal limitations, which could be consider to apply to tomato production in salt-affected land.

The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (Nicotiana tabacum L.): Nonlinear Effects of NaCl Concentrations

Salinity impacts important processes in plants, reducing their yield. The effect of salinity on the cytosolic pH (pHcyt) has been little studied. In this research, we employed transgenic tobacco plants expressing the pH sensor Pt-GFP to investigate the alterations in pHcyt in cells across various root zones. Furthermore, we examined a wide spectrum of NaCl concentrations (ranging from 0 to 150 mM) and assessed morphological parameters and plant development. Our findings revealed a pattern of cytosolic acidification in cells across all root zones at lower NaCl concentrations (50, 100 mM). Interestingly, at 150 mM NaCl, pHcyt levels either increased or returned to normal, indicating a nonlinear effect of salinity on pHcyt. Most studied parameters related to development and morphology exhibited an inhibitory influence in response to NaCl. Notably, a nonlinear relationship was observed in the cell length within the elongation and differentiation zones. While cell elongation occurred at 50 and 100 mM NaCl, it was not evident at 150 mM NaCl. This suggests a complex interplay between stimulating and inhibitory effects of salinity, contributing to the nonlinear relationship observed between pHcyt, cell length, and NaCl concentration.

Improving salt tolerance threshold in common bean cultivars using melatonin priming: a possible mission?

Common bean (Phaseolus vulgaris L.) is mostly cultivated on marginal soils where salinity is a key stress. The focus of the present study was to evaluate the efficiency of melatonin priming and hydro priming on germination, growth parameters, antioxidant defense system and tolerance threshold in seven common bean cultivars grown under different levels of salinity stress. The experiments were conducted as a factorial arrangement based on a completely randomized design with six replicates. The results showed that salinity levels decreased radicle and hypocotyl lengths, seedling vigor index, and salinity tolerance threshold under Petri dish conditions. However, melatonin priming alleviated the inhibitory effects of salinity and enhanced salt tolerance threshold of all the cultivars. The highest tolerance thresholds of Dorsa, Almas, Koosha, and Pak cultivars were obtained under 100 µM melatonin treatment, while in Sayad, Shekoofa, and Derakhshan, 20 µM melatonin caused higher thresholds. Results of the greenhouse experiment showed that melatonin priming increased salt tolerance threshold, but it reduced malondialdehyde content and hydrogen peroxide accumulation. It also induced a higher increase in relative shoot dry weight of the cultivar Dorsa than the other cultivars. The cultivars Dorsa and Shekoofa treated with 100 and 20 µM, respectively, maintained a lower MDA content and Na+ concentration and a higher K+/Na+ ratio in shoot and root, N concentration and salt tolerance threshold than the other cultivars under salinity stress. These results suggest that seed priming with melatonin enhances salinity tolerance by stimulating antioxidant activities, alleviating oxidative damage and enhancing plant dry weight.

THE POTENTIAL USE OF Ca(NO3)2 TO IMPROVE SALINITY TOLERANCE IN DATE PALM (Phoenix dactylifera L.)

The study was conducted to investigate the impact of Ca(NO3)2 on different levels of salt-stress in date palm. Three-years-old date palm plants were subjected to four NaCl levels: 50, 100, 150 and 200 mM. The saline solutions were supplemented with 0, 5, 10 and 20 mM Ca(NO3)2. The combined NaCl/Ca(NO3)2 treatments were conducted over a period of 10 weeks. Control plants were only subjected to the four salinity levels with no Ca (NO3)2 addition. Results showed an inhibitory effect of salinity on almost all plants' parameters under investigation, mainly the accumulation of ions such as N, K, Ca, plant dry weight, chlorophyll and net photosynthesis rate. Addition of Ca (NO3)2 in the solution was more beneficial when added in a moderate concentration (10 mM) compared to lower (5 mM) and higher (20 mM) under all salinity levels. The addition of 10 mM Ca (NO3)2 noticeably enhanced chlorophyll content under 50 mM Na Cl (2.5 mg/100 cm2) and 150 mM NaCl (2 mg/100 cm2). In addition, 10 mM Ca (NO3)2 reduced the accumulation of Na and Cl in plant parts. For instance, in trees subjected to 10 mM Ca (NO3)2, Cl content in leaves and stems under 50 mM Na Cl were 0.23% and 0.65%, respectively. On the other hand, Cl content under 100 mM Na Cl and 200 mM Na Cl were lower compared to their corresponding control treatments. It seems that the use of Ca (NO3)2 had ameliorative effects on salt-stressed date palm plants when used with moderate concentrations.

Inhibitory Effect of Salinity and Humic Acid on the Performance of Anaerobic Ammonium Oxidation Process and Recovery of Anammox Activity

The anaerobic ammonium oxidation (Anammox) process can effectively treat the landfill leachate. However, the influences of complex components in landfill leachate on anammox bacteria were insuffici...

Salinity effect on freshwater Anammox bacteria: Ionic stress and ion composition

The biggest challenge to apply Anammox to treat wastewater with elevated salt content is the inhibitory effect of salinity on freshwater Anammox bacteria (FAB). Most of the research into salinity inhibition has focused on the osmotic pressure effect, while the inhibitory effect and its mechanisms induced by ion composition are poorly understood. In this study, the individual and combined effect of NaCl, KCl and Na2SO4 on FAB (>99% belonging to Ca. Brocadia genera) were systematically investigated by batch tests. The corresponding responses of mRNA abundance of three functional genes (including nitrite reductase gene (nirS), hydrazine synthase gene (hzsB) and hydrazine dehydrogenase gene (hdh)) under different salt conditions were analyzed. The results indicated that NaCl, KCl and Na2SO4 have different inhibition effects, with the 50% inhibition at 0.106, 0.096 and 0.063 M, respectively. The combined inhibition of NaCl+KCl and NaCl+Na2SO4 on FAB were both synergistic; while the combined inhibition of NaCl+KCl+Na2SO4 was additive. The responses of mRNA (of genes: nirS, hzsB and hdh) suggested NaCl inhibited the transport of ammonium; Na2SO4 inhibited both nitrite and ammonium transport; high salinity inhibited functional enzyme activity. These results suggest both ionic stress and ion composition contributed to the observed inhibition.

Tissue-specific modulation of metabolism and nutrients acquisition through seed priming with sodium selenate confers salt tolerance in wheat

ABSTRACT The experiment was conducted to appraise whether exogenously applied selenium (Se) could alter nutrients acquisition and metabolism to mitigate the toxic effects of salinity in wheat. Seeds of two contrasting wheat (Triticum aestivum L.) cultivars (Auqab-2000 and Seher-2006) were pre-treated with different concentrations (0, 50, 100 µM) of sodium selenate for 12 h. The pots were irrigated with different NaCl concentrations (0 and 150 mM) applied through Hoagland’s nutrient solution before sowing. Salinity reduced fresh and dry mass and grain yield, but the exogenous Se restored the growth and increased grain yield. Further, salt stress decreased the concentrations of K+, Ca2+, P, N, Fe and Se while increased the concentration of Na+ and Cl‒ in the shoots and roots of wheat. The exogenous Se increased K+, Ca2+, N, Fe and Se concentrations while decreased Na+, Cl− and P, and thus mainly reversed the inhibitory effects of salinity on nutrients uptake. Further, exogenous Se increased the accumulation of proline and glycinebetaine under salinity. Overall, Se-priming proved an effective shotgun technique to mitigate the detrimental effects of salinity possibly through its beneficial effects on tissue-specific ion acquisition and accumulation of osmoprotectants in wheat.

Synergistic removal of copper and tetracycline from aqueous solution by steam-activated bamboo-derived biochar

Steam-activated biochar (SBC) was prepared and showed excellent performance for synergistic removal of Cu2+ and tetracycline (TC). The adsorption capacity of SBC and mutual effect of TC and Cu2+ were investigated via single and binary system and the adsorption isotherm. The adsorption capacity of TC was significantly enhanced when it coexisted with Cu2+. Likewise, increased amounts of Cu2+ were adsorbed in the presence of TC. The presence of NaCl exerted a negative influence on the adsorption of Cu2+, while the inhibitory effect of salinity on TC was neutralized by bridge enhancement in the binary system. Bridge enhancement and site competition were involved in the synergistic removal of TC and Cu2+. Considering the stable application in simulated and real water samples, SBC showed great potential for synergistic removal of antibiotics and heavy metals.

A preliminary study on salt tolerance of some barley genotypes

In this study, salt (NaCl) tolerance of five barley genotypes (Avcı-2002, Aydanhanım, Bülbül-89, Orza-96, Tarm-92) was investigated. For this purpose, plants were exposed to different salt concentrations (0 mM (control), 100 mM, 200 mM and 300 mM) and some basic growth parameters (root length, shoot length total plant length, fresh weight, dry weight) were determined on the 18 d old plants. Tolerance indices for each genotype was calculated on the basis of root and shoot length. Our results showed that salt stress inhibited root and shoot growth in all genotypes significantly except Bülbül-89. Inhibition degree of root and shoot growth was found to be proportional to the salt concentrations applied. Maximum inhibitory effect of salinity on root and shoot growth, for example, was observed in Avcı-2002 at 300 mM salinity level, while Bülbül-89 was less affected by salt stress. In addition, we found that salt stress disturbed water relation between barley genotypes and growth medium dependent on the organ type, as demonstrated by more severe inhibition in shoot fresh weight as compared to root fresh weight. These results may show that salt stress reduced translocation of water from roots to shoots rather than water uptake from soil in barley genotypes used in this study. Changes in dry weight of root and shoot barley genotypes indicated that salt stress reduced biomass accumulation in roots. As a result, we concluded that Bülbül-89 is the most tolerant barley cultivars while Avcı-2002 is the most susceptible one, as indicated by the calculated tolerance indices.

Transport of barium in fractured dolomite and sandstone saline aquifers

To understand the advective-dispersive transport of Ba in fractured dolomite and sandstone saline aquifers, we conducted core-flooding experiments and reactive transport simulations. We used intact and synthetic fractured dolomite and sandstone cores collected from formations where hydraulic fracturing (HF) wastewater is disposed in Oklahoma, USA. The core-flooding experiments were conducted using saline water containing typical concentrations of NaCl (90 g/L), Ca (5 g/L), Mg (1 g/L), and Ba (100 mg/L) in HF wastewaters. At typical concentrations of NaCl, Ca, and Mg in HF wastewater, our experimental results show similar Ba transport rates in both intact and fractured dolomites but faster Ba transport rates in intact than in fractured sandstones. We found a match between measured and simulated breakthrough curves of Ba in intact and fractured sandstones. This supports the hypothesis that the inhibitory effect of salinity on Ba sorption increases Ba transport through matrix pores bordering the fracture while reducing its transport through the fracture. This is reflected by a reduction of the overall rate of Ba transport through fractured dolomites and sandstones. We found that the effect of salinity in retarding Ba transport through fractured dolomites and sandstones increases with increased matrix porosity and/or fracture aperture size. We implemented the multiple interacting continua (MINC) method developed for modeling fluid flow in fractured porous media to successfully capture the effect of salinity, matrix porosity and fracture aperture size on Ba transport in fractured sandstones. The measured and simulated results have significant implications on efforts of field-scale simulations of Ba transport in dolomite and sandstone saline aquifers where HF wastewater is disposed.

Nitrogen removal performance of municipal reverse osmosis concentrate with low C/N ratio by membrane-aerated biofilm reactor

A membrane-aerated biofilm reactor was employed to investigate the nitrogen removal of one typical municipal reverse osmosis(RO) concentrate with a high total nitrogen (TN) concentration and a low C/ N ratio. The effects of operational conditions, including the aeration pressure, the hydraulic retention time and the C/N ratio, on the systematic performance were evaluated. The nitrogen removal mechanism was evaluated by monitoring the effluent concentrations of nitrogen contents. Furthermore, the microbial tolerance with elevated salinity was identified. The results indicated that the optimal TN removal efficiency of 79.2% was achieved of the aeration pressure of 0.02 MPa, hydraulic retention time of 24 h, and the C/N ratio of 5.8, respectively. It is essential to supplement the carbon source for the targeted RO concentrate to promote the denitrification process. The inhibitory effect of salinity on denitrifying bacteria and nitrite oxidizing bacteria was significant, revealing the limited TN removal capacity of the conditions in this work. The TN removal efficiency remained more than 70% with the addition of salt (NaCl) amount below 20 g/L. This work preliminarily demonstrated the MABR feasibility for the nitrogen removal of municipal RO concentrate with low C/N ratio and provided technical guidance for further scale-up application.

Effects of different salinity levels on nitrification processes in sediments of Minjiang River Estuary, China

Two estuary wetlands in Minjiang River, Shanyutan and Daoqingzhou, were selected as the research objects. Wetland sediments were collected to examine the effects of different salinity levels on nitrification processes in the wetland with a culture experiment. The results showed that the nitrification rate of sediment in Minjiang River estuary wetland was generally low. The highest nitrification rate of sediment in the Shanyutan wetland was 0.193 mg·kg-1·d-1, while that in the Daoqingzhou wetland did not exceed 0.050 mg·kg-1·d-1. In the low salinity level (5), the decrease of nitrification rate was attributed to the restraint of nitrifying bacteria activities. The nitrification rate slightly increased with the increases of salinity (10), but was still lower than the initial value. This would be owed to the fact that the inhibitory effect of salinity on the activities of aerobic bacteria was strengthened, which reduced the rate of NH4+-N production, and thus resulted in a decrease of the contribution of aerobic ammonification bacteria to the apparent nitrification rate. There were regional variations in the responses of sediment nitrifying activity to salinity. In saltwater wetland (Shanyutan wetland), the adaptability of microbes in sediment to salinity was stronger, so that the nitrification activity in high salinity conditions was still higher. While in the freshwater wetland (Daoqingzhou wetland), the adaptability of the sediment to the salinity change was relatively lower, with a lower nitrification activity of the sediment in high salinity than in middle salinity. Acidic condition was the main reason for the low nitrification in the sediments of Minjiang estuary wetland. The nitrification rate and nitrification activity of the two wetlands increased first and then decreased with the duration of the culture experiment, which was driven by combined effects of initial NH4+-N concentration, oxygen content and denitrifying.

NONOSMOTIC EFFECT OF POLYETHYLENE GLYCOL ON PERCENT SURVIVAL AND GROWTH OF RICE

Salinity is one of the major environmental factors limiting crop productivity. For this reason, two greenhouse experiments were conducted in Faculty of Agriculture, Cairo University, Egypt, during the year 2015 using two rice varieties to evaluate the effects of various levels of osmotic stress caused by polyethylene-glycol 6000 (PEG) and NaCl. Furthermore, it was tested whether the inhibitory effect of salinity on growth, sodium and chloride concentration by two different varieties was greater under NaCl or PEG treatment. The first experiment was undertaken to separate osmotic and ionic aspects of salinity damage to rice (Oryza sativa L.).Seedlings of IR28 (salt-sensitive) and Nona Bokra (salt-tolerant) rice varieties were transferred to salinized nutrient solution containing 85 mol m-3NaCl (-3.0 bars) with or without PEG 6000 (-2.0 bars, 45 gL-1). Plants were grown up to 30 days in the salinized solutions. The second experiment was designed to determine the effect of salinity (85 mol m-3NaCl) with or without PEG 6000 (-0.5 bar, 11 g L-1)on growth, uptake and transport of sodium and chloride in two rice varieties differing in salt tolerance. The results indicated that survival of salt-tolerant variety (Nona Bokra) was increased significantly by adding PEG (-2.0 bars). The addition of PEG also reduced the rate of death of rice plants compared with NaCl alone. Also, data showed that PEG 6000 (0.5 bar, 11 gL-1) reduce sodium concentration in root of IR28 and Nona Bokra but its effect upon sodium concentration in shoot of the two varieties was more pronounced than the reduction of Na+ concentration in root. Highly significant differences were obtained between zero and 11 gL-1 PEG. The result of this study is strongly indicated that addition of PEG dramatically lessened the toxicity of NaCl to rice seedlings.

Tuz Stresi Altında Çimlendirilen Arpa Tohumlarında Borik Asit Uygulamasının Sitogenetik Etkisi

The effect of exogenous boric acid on the mitotic activity and chromosomal aberrations in root tip meristems of barley (Hordeum vulgare L. cv. ‘Bulbul 89’) seeds germinated under salt stress was investigated. The inhibitory effect of salinity on mitotic index increased with increasing salt concentrations (0.00- control, 0.25, 0.275 and 0.30 M, molar NaCl) as compared to control group. The mitotic index greatly reduced at the highest salt level (0.30 M NaCl). At the same time, the frequency of chromosomal aberrations increased significantly in parallel with the salt concentration rise. It was determined that application of alone boric acid (0,005 µM) was not a prohibitive effect on mitotic index as compared with the barley seeds germinated in distilled water (C, control). Also, the frequency of chromosomal aberrations in application of boric acid increased approximately three-fold according to the control group. Boric acid+different NaCl concentrations simultaneously application remarkably increased the negative effects of salinity on the mitotic activity. However, boric acid application, particularly at high salt concentrations showed a perfect successful in alleviating of the detrimental effect of salinity on the chromosomal aberrations

Inhibitory effect of salinity on the photocatalytic degradation of three sulfonamide antibiotics

This study investigates the effects of two salinity treatments (20‰ and 34‰) with seawater on the photocatalytic degradation of sulfonamide antibiotics (SAs). Three SAs, sulfamethoxazole (SMX), sulfamonomethoxine (SMM), and sulfadimethoxine (SDM), were respectively added (50 mg L−1) to saline water containing 100 mg L−1 of TiO2, and irradiated with ultraviolet (UV) light. The results revealed that the photocatalytic degradation and mineralization of the SAs decreased with an increasing level of salinity treatments. The residual concentrations of the chemical oxygen demand (COD) and dissolved organic carbon (DOC) in the solutions also revealed that SA mineralization decreased with the salinity treatments. The toxicity of the SA solutions to the bioluminescent bacteria, Vibrio fischeri, in the photocatalytic processes increased with the irradiation times, but decreased with an increasing level of salinity treatments. Further study indicated that the inhibitory effects of cations in seawater were Ca2+ > Mg2+ > K+ > Na+, whereas for anions they were I− > SO42− > Cl− > Br−.

The inhibitory effect of salinity on the photosynthetic machinery of mustard plant and the alleviation of this effect by ethylene

The inhibitory effect of salinity on the photosynthetic machinery of mustard plant and the alleviation of this effect by ethylene

INTERACTIVE EFFECTS OF SALINITY AND CALCIUM ON HYDRAULIC CONDUCTIVITY, OSMOTIC ADJUSTMENT, AND GROWTH IN PRIMARY ROOTS OF MAIZE SEEDLINGS

ABSTRACT Increasing the calcium ion activity in salinized root media has often been shown to ameliorate the inhibitory effect of salinity on plant growth. In order to better define the biophysical mechanisms involved in these growth responses, we investigated the interactive effects of salinity and calcium on hydraulic conductivities and osmotic potential gradients in roots of maize seedlings. The length of the primary roots was reduced by 54% after 4 days of growth in 0.1-strength Hoagland solution salinized with 100 mM NaCl and by 20% when 10 mM calcium was also added to the salinized root medium. Roots showed 69% osmotic adjustment in response to salinization, with or without extra calcium in the root medium. The mean hydraulic conductivity, L, of the apical 4 cm of maize seedling roots was determined by assaying osmotically-induced backflow. The assay was sensitive enough to detect reductions in L induced by lowering the assay temperature from 27 to 14°C. These reductions in L exceeded those caused by...

Chromotoxic effects of exogenous hydrogen peroxide (H2O2) in barley seeds exposed to salt stress

The effect of exogenous hydrogen peroxide (H2O2) on mitotic activity and chromosomal aberrations in root tip meristems of barley (Hordeum vulgare L. var. Tokak 157/37) germinated under salinity was analyzed. The inhibitory effect of salinity on mitotic index and the frequency of chromosomal aberrations increased with increasing salt concentration (0.00 control, 0.35, 0.40, 0.45 M, molal NaCl). The frequency of chromosomal aberrations of seeds germinated in medium with 0.40 M NaCl after pretreatment with H2O2 (30 μM, micromolal) was significantly higher than the control group. The highest concentration of NaCl (0.45 M) together with H2O2 caused total inhibition of germination. In this study, the intention was to determine the performance of H2O2 in alleviating detrimental effect of salt stress on mitotic activity and chromosomal aberrations. However, H2O2 did not reduce the detrimental effect of NaCl on these parameters. Also, it caused higher chromotoxic effect compared to those of control groups.

Salinity‐induced changes in essential oil, pigments and salts accumulation in sweet basil (Ocimum basilicum) in relation to alterations of morphological development

The objective of the project was to study salinity‐induced effects on essential oil, pigments and salts accumulation in sweet basil (Ocimum basilicum, the cultivar Perrie) in relation to the alteration of plant morphological development and yield production. Hydroponically grown plants were exposed to one of six NaCl concentrations (1, 25, 50, 75, 100 and 130 mM NaCl). Inhibitory effects of salinity on biomass production of the shoot and the root, and area of individual leaves were apparent already under cultivation with 25 mM NaCl. Elevation of salinity from 1 to 100 mM NaCl induced 63% and 61% reductions in fresh and dry herb biomass production, respectively. The stress‐induced reduction of foliage biomass sourced mainly from inhibition of leaf area development rather than reduction of internode and leaf number. Cl and Na concentrations in the leaves, stems and roots increased with elevation of NaCl concentration in the cultivation solution. While the extent of Cl accumulation was leaves>stems>roots, Na was largely excluded from the leaves and was preferentially accumulated in roots and the stems, potentially accounting for the moderate sensitivity of the leaf tissue to salinity. Salt stress increased the contents of essential oil and carotenoids in the leaves that may further account for the moderate sensitivity of sweet basil to salinity and suggest a potential for agro‐industrial production. A twofold increase in both carotenoid concentration and the percent of essential oil in the fresh tissue was observed by elevation of the salinity from 1 to 130 mM NaCl. Overall, the stress induced increase of the percent of essential oil in the tissue in the salinity range 1–75 mM NaCl was about 50%, and thereby compensated for the similar reduction of biomass production in this salinity range, so that oil production on per plant basis was not reduced by salinity.

Formation of Plant Communities of the Newly Created Wetland in Modern Yellow River Delta

To explore the cause of plant vegetation distributed on the newly created wetland in estuary of modern Yellow River Delta, we took field-based observation of the wetland plant diversity of the Yellow River Delta, Shandong, China. Based on the results of field investigation, we studied the effects of salinity on germination characteristics of four dominant species in wetland of Yellow River Delta, i.e. Suaeda salsa, Apocynum venetum, Phragmites australis and Typha orientalis.The germination conditions of the four dominant emerging plants at 0, 50, 100, 150, 200, 250 and 300mM NaCl were studied, the results indicated that all species had a trend of decreased germination percentage and rate of germination with the increase of salinity levels, but the inhibitory effect of salinity on the germination percentage of each was different. S. salsa and A. venetum had higher tolerance of salinity than P. australis and T. orientalis. Germination percentage and germination rate of seeds of the four species were significantly affected by salinity.In conclusion, the results of seeds germination of the four dominant species in the newly created wetland were accorded with the results of field investigation of plant community distribution along the gradients of environmental salinity, and salinity is one of the most pivotal factors limited the distribution of the plant vegetation in the newly created wetland of Yellow River Delta.