Ulcer Inhibition Research Articles

Bio-fabrication of biologically active copper nanocomposite

Green routes for nucleation of nanostructures are recently applied for the biomedical applications, due to their affinity and feasibility. For instance, copper nanostructures are applicable for inhibition of microbial infections and ulcers. Hereby, this work is aimed at investigation for the possible contribution of Sidr honey (SH) in the formation of copper based-nanocomposites (Cu@SH). The overall results affirmed that, under the optimal experimental conditions, spherical shaped Cu@SH nanocomposites with quite small particle size of 16.2–30.1 nm were successfully clustered. The bio-fabricated Cu@SH nanocomposites showed superior antimicrobial action against all of the examined strains, whereas, the most potent inhibition was obtained against St. aureus (11 mm), E. Coli (17 mm) &C. albicans (14 mm). The nanocomposite that was prepared with higher concentration of copper precursor is exhibited with the highest percentage of cancer cell inhibition (77 %) and highest anti-inflammation action (NO percentage 89 %). In summarization, Cu@SH nanocomposites showed high affinity to be concurrently applicable as therapeutic drugs for the microbial infectious, cancer and inflammation diseases.

A stimuli-responsive keratin functionalized PEI coated baicalin nano-hydrogels for modulated gastric ulcer therapy

Nano-hydrogel system conjugated with polymer like polyethyleneimine offers a significant attention in the field of healthcare owing to their significant pH-specific swelling behaviour in acidic medium. The focus of this research work was to fabricate a stable and non-toxic, keratin-PEI coated nano-hydrogel formulation exhibiting synergistic effect for gastric ulcer treatment. K-PEI-BL nano-hydrogel shows particle size ∼500 nm with polydispersity index 0.473 ± 0.04, zeta potential of 15.4 ± 6.06 mV, entrapment efficiency of 91.94 ± 0.04 % and in vitro controlled release of 72.78 ± 0.52 % for 24 h in pH 1.2. Scanning electron miscroscopy (SEM), Transmission electron microscopy (TEM) images confirmed the morphological structure of double coated nano-hydrogel particle. Ex-vivo mucoadhesive study of keratin-PEI coated-BA-NH formulation at gastric ulcer site exhibited 21 h and 15 min as a residence time in comparison to uncoated BA-NH formulation. In-vivo analysis displayed improvement in ulcer treatment compared to negative control group with 86.46 % and 71.86 % inhibition of ulcer in standard ranitidine and K-PEI-BL nano-hydrogel treatment groups, respectively. This research study concluded that K-PEI-BL nano-hydrogel formulation with 15 mg/kg dose exhibited anti-ulcer actions by reducing the severity of gastric abrasions.

Rosuvastatin repurposing for prophylaxis against ethanol-induced acute gastric ulceration in rats: a biochemical, histological, and ultrastructural perspective.

Ethanol (EtOH) consumption is frequently associated with acute and chronic gastrointestinal disorders. Rosuvastatin (RSV), a third-generation statin, has demonstrated certain biological functions beyond its lipid-lowering properties. This study is designed to explore the gastroprotective impact of RSV in a rat model of EtOH-induced gastric ulceration in a dose-dependent manner through the evaluation of oxidant/antioxidant biomarkers, inflammatory myeloperoxidase (MPO) enzyme activity, and prostaglandin E2 (PGE2) levels in gastric tissues, along with histopathological examination of the gastric tissues. Therefore, 40 adult male rats were randomly divided into five equal groups as control, EtOH (gastric ulcer), RSV-low dose plus EtOH and RSV-high dose plus EtOH. The EtOH rat model of gastric ulceration was achieved by intragastric administration of a single dose of EtOH. Seven days before EtOH administration, rats were orally administered either omeprazole (20mg/kg/day) or RSV (10mg/kg/day or 20mg/kg/day). RSV administration enhanced the antioxidant glutathione reduced, countered oxidative malondialdehyde, augmented cytoprotective PGE2, suppressed inflammatory MPO enzyme activity in gastric tissues, decreased ulcer index scoring, increased the percentage of ulcer inhibition, and reversed the associated histological and ultrastructural abnormalities, additionally, RSV treatment resulted in weak positive nuclear staining for the inflammatory nuclear factor kappa B in a dose-dependent manner. It is concluded that RSV demonstrates gastroprotective potential, attributable at least in part, to its antioxidant and anti-inflammatory properties, as well as its ability to promote ulcer protection through the maintenance of mucosal content and PGE2 levels. Thus, RSV therapy emerges as a safe option for patients with gastric ulcers.

Gastroprotective Effect of Abelmoschus esculentus (Ex-Maradi Okra Fruit Variety) Against Ethanol-Induced Ulcers in Rats

Study’s Novelty/Excerpt The study evaluates the gastroprotective properties of the Ex-Maradi variety of Abelmoschus esculentus (okra) against ethanol-induced gastric mucosal damage in Wister rats, specifically comparing fresh okra mucilage (FOM) and dried okra powder (DOP). The significant ulcer inhibition and antioxidant effects of DOP, particularly at a 500 mg/kg dosage, indicate its potential as a natural therapeutic agent for peptic ulcer disease. The research highlights the potential for developing green anti-ulcer formulations derived from okra, expanding the scope of nutraceutical applications for this commonly consumed vegetable. Full Abstract Peptic ulcer disease, a notable gastrointestinal disorder, results from an imbalance between gastric acid secretion and the factors maintaining gastric mucosal integrity. Abelmoschus esculentus, commonly known for its mucilaginous and nutraceutical properties, also exhibits an antacid effect. This research aimed to examine the antacid properties of fresh okra fruit mucilage (FOM) and dried okra fruit powder (DOP) of the Ex-Maradi okra fruit variety against ethanol-induced gastric mucosal damage in Wister rats. Rats were randomly assigned to seven groups consisting of six rats each. Rats in the FOM and that of the DOP group were pretreated orally with 250 and 500 mg/kg body weight of the FOM and DOP, respectively; the drug control (DC) group was pretreated orally with 20 mg/kg body weight of Cimetidine while the normal control (NC) group and the ulcer control (UC) group were pretreated orally with normal saline (2 mL/kg body weight). All the treatments were done for seven days before the induction of the ulcer. Ulcer index (UI), percentage inhibition (PI), gastric volume, gastric pH, total acidity, and total antioxidant power (TAP) were evaluated to assess the gastro-protective effect of the FOM and DOP in the rats. Both FOM and DOP groups demonstrated significant (P < 0.05) protection with a low ulcer index (2.41 ± 0.12) and high ulcer inhibition (75.6 %) against the damaging effect of ethanol on the gastric mucosa of the animals. Additionally, DOP also exhibited a strong antioxidant effect with a good percentage inhibition value (56.53 ± 2.1%) compared to the ulcer control group. These results were further supported by the histopathological findings from the rats’ stomachs. In conclusion, the Ex-Maradi okra fruit, especially the DOP500, demonstrated significant (P < 0.05) gastro-protective effects and maintained a relatively intact and continuous epithelial surface of the rats’ stomachs. Overall, its gastroprotective effects may be possibly mediated by its potential to modulate the antioxidant system and gastric acid levels. Hence, the dried okra fruit could be suitable for the development of green anti-ulcer formulations.

Evaluation of antiulcer potential of tambulin and ombuin isolated from Zanthoxylum armatum

ObjectivesZanthoxulym armatum fruit is traditionally used as carminative, anthelmintic, stomachic, and to relieve gastritis. It is commonly found in Nepal, Uttarakhand, and the Himalayan region of India. Tribal peoples used this fruit for various purposes and also as a spice ingredient. The present study aims to investigate the antiulcer potential of the fruit extract, and its isolated compounds. Materials and methodsFruits were extracted with successive extraction methods with hexane, ethyl acetate, and butanol. All the fractions were evaluated for phytochemical investigations. Further, the ethyl acetate fraction, tambulin, and ombuin were tested for antiulcer activity induced by ethanol and pylorus ligation-induced methods. The IL-1β, TNF-α, and IL-6 cytokines were also studied followed by histopathology. ResultsIt has been demonstrated that the ethyl acetate fraction possesses a major class of secondary metabolites. No acute toxicity was observed, as all the organs (heart, kidney, lung, stomach, and liver) were found in normal range and no significant weight variation was found. The antiulcer activity was performed at two different doses of all samples. Tambulin possesses the most significant activity at the dose of 50 mg/kg. Ulcer index and percent ulcer inhibition of tambulin were found to be 2.8 ± 0.6 mm2 and 69.5 ± 0.18 % in pylorus –ligated model whereas 5.2 ± 0.7 mm2 and 70.2 ± 0.15 in the ethanol-induced model. Histopathology results also support the above-mentioned data. ConclusionTambulin, a flavonoidal compound isolated from fruits of Z. armatum was found to be useful in the management of ulcers. Ethylacetate extract and ombuin also possess dose-dependent activity.

Co-Amorphization of Acemetacin with Basic Amino Acids as Co-Formers for Solubility Improvement and Gastric Ulcer Mitigation.

Acemetacin (ACM) is a new non-steroidal anti-inflammatory drug with anti-inflammatory, analgesic, and antipyretic effects. However, the poor water solubility and gastrointestinal side effects limit its use. Recently, the co-amorphous (CAM) strategy has attracted great interest to improve solubility for poorly water-soluble drugs, and basic amino acids have the potential to protect the gastrointestinal tract. In order to develop a highly efficient and low-toxic ACM formulation, we prepared ACM CAM systems, with basic amino acids (lysine, arginine, and histidine) as co-formers, using a cryo-milling method. The solid-state behaviors of the ACM CAM systems were characterized by polarizing light microscopy, differential scanning calorimetry, and powder X-ray diffraction. Fourier transform infrared spectroscopy and molecular docking were carried out to understand the formation mechanism. Moreover, the gastro-protective effects of ACM CAM systems were evaluated in a rat gastric ulcer model. The results demonstrated that the CAM systems improved the dissolution rates of ACM compared with the neat amorphous counterpart. Furthermore, ACM CAM systems are significantly effective in mitigating the ACM-induced gastric ulcer in rats, and the ulcer inhibition rates were almost 90%. More importantly, this study provided a useful method for mitigating drug-induced gastrointestinal damage and broadened the applications of drug-amino acid CAM systems.

Research on the mechanism of Guanyu Zhixie Granule in intervening gastric ulcers in rats based on network pharmacology and multi-omics.

Guanyu Zhixie Granule (GYZXG) is a traditional Chinese medicine compound with definite efficacy in intervening in gastric ulcers (GUs). However, the effect mechanisms on GU are still unclear. This study aimed to explore its mechanism against GU based on amalgamated strategies. The comprehensive chemical characterization of the active compounds of GYZXG was conducted using UHPLC-Q/TOF-MS. Based on these results, key targets and action mechanisms were predicted through network pharmacology. GU was then induced in rats using anhydrous ethanol (1 mL/200 g). The intervention effects of GYZXG on GU were evaluated by measuring the inhibition rate of GU, conducting HE staining, and assessing the levels of IL-6, TNF-α, IL-10, IL-4, Pepsin (PP), and epidermal growth factor (EGF). Real-time quantitative PCR (RT-qPCR) was used to verify the mRNA levels of key targets and pathways. Metabolomics, combined with 16S rRNA sequencing, was used to investigate and confirm the action mechanism of GYZXG on GU. The correlation analysis between differential gut microbiota and differential metabolites was conducted using the spearman method. For the first time, the results showed that nine active ingredients and sixteen targets were confirmed to intervene in GU when using GYZXG. Compared with the model group, GYZXG was found to increase the ulcer inhibition rate in the GYZXG-M group (p < 0.05), reduce the levels of IL-6, TNF-α, PP in gastric tissue, and increase the levels of IL-10, IL-4, and EGF. GYZXG could intervene in GU by regulating serum metabolites such as Glycocholic acid, Epinephrine, Ascorbic acid, and Linoleic acid, and by influencing bile secretion, the HIF-1 signaling pathway, and adipocyte catabolism. Additionally, GYZXG could intervene in GU by altering the gut microbiota diversity and modulating the relative abundance of Bacteroidetes, Bacteroides, Verrucomicrobia, Akkermansia, and Ruminococcus. The differential gut microbiota was strongly associated with serum differential metabolites. KEGG enrichment analysis indicated a significant role of the HIF-1 signaling pathway in GYZXG's intervention on GU. The changes in metabolites within metabolic pathways and the alterations in RELA, HIF1A, and EGF mRNA levels in RT-qPCR experiments provide further confirmation of this result. GYZXG can intervene in GU induced by anhydrous ethanol in rats by regulating gut microbiota and metabolic disorders, providing a theoretical basis for its use in GU intervention.

Evaluation of &lt;i&gt;Hibiscus sabdariffa&lt;/i&gt; Aerial Parts against Pyloric Ligation-induced Ulcers in Experimental Rats

The sloughing off inflammatory dead tissue is characteristic of ulcers, which are open sores of the skin or mucous membrane. Peptic ulcers are erosive lesions on the stomach or duodenal lining. The drive of the study was to evaluate the effectiveness of Hibiscus sabdariffa aerial parts extracts against pylorus ligation-induced ulcers. The extracts were prepared by maceration using ethanol and double distilled water. In the treatment schedule, Groups A and B were considered negative and standard (Lansoprazole 8 mg/kg b. wt, p.o.) controls. Groups C, D, E, and F as low (200 mg/kg b. wt, p.o.) and high (400 mg/kg b. wt, p.o.) dose treatment groups, received EEHS and AEHS, respectively. Gastric content, pH, free and total acidity, ulcer index, and inhibition of ulcer (%) were assessed. Following administration of both extracts, measurements of stomach content, free and total acidity, and ulcer index decreased. Additionally, pH increased, and a greater percentage of ulcers were inhibited. It was discovered that the ethanolic extract had significantly higher anti-ulcer action than the aqueous extract.

Juglans regia L. fruit pellicle extract-based bioreduction of silver nanoparticles: Structural features and in vivo therapeutic effects against ethanol-induced peptic ulcers

Context: Peptic ulcers are a challenging problem. Silver nanoparticles (AgNPs) play a substantial role in bioactivity and have distinct characteristics. There are several biomedical uses for bioreduction nanotechnology. Historically, traditional healthcare has employed Juglans regia L. Aims: To evaluate how well fruit pellicle extract-derived bio-reduced silver nanoparticles (AgNP/FP) can treat peptic ulcers. Methods: Ultraviolet spectra, dynamic light scattering, transmittance electron microscopy, and Fourier transform infrared were used to characterize AgNPs and AgNP/FP. AgNPs and extracts were compared to omeprazole in ethanol-induced stomach ulcers in mice. Six groups of 36 mice were created at random. AgNPs were given orally to the experimental groups, using 300 mg/kg as a dose. The reference proton pump inhibitor, omeprazole, was employed as a control. Results: As evidenced by a falling ulcer index and an increase in the percentage of ulcer inhibition, bio-reduced AgNPs decreased the adverse effect of ethanol-induced stomach injury. Reduced mucosal damage and hemorrhagic lesions, necrobiotic alterations in the stomach epithelium, submucosal edema, and blood vessel congestion were all indicators of substantially reduced ethanol-induced gastric lesions and greater anti-inflammatory results. Conclusions: Fruit pellicle extract-derived bio-reduced silver nanoparticles have an efficacy similar to proton pump inhibitors like omeprazole and a lower incidence of possible side effects, making them a safe herbal therapy for ulcers.

Gastroprotective effects of polysaccharides from purple sweet potato (Ipomoea batatas (L.) Lam) on an ethanol-induced gastric ulcer via regulating immunity and activating the PI3K/Akt/Rheb/mTOR pathway.

The study aimed to investigate the alleviation of an ethanol-induced gastric ulcer in mice by apolysaccharide (PSP) from purple sweet potato (Ipomoea batatas (L.) Lam) and explore the mechanism. The anti-ulcer activity was determined by histopathological evaluation, total gastric acidity, pepsin activity, gastric ulcer index and gastric ulcer inhibition rate. The expression levels of inflammatory factors were detected using ELISA. A special protein meter was used to detect the content of immunoglobulin lgM, immunoglobulin lgG, and complements C3 and C4 in the serum of mice. The expression of CD4+/CD8+ lymphocyte subsets of mice was detected using flow cytometry. Western blot analysis was used to examine the effect of PSP on the PI3K/Akt/Rheb/mTOR pathway. The results showed that PSP could effectively reduce the total gastric acidity, pepsin activity, and the index and inhibition rate of gastric ulcers. At the same time, PSP could significantly increase the levels of immunoglobulins (lgG and lgM) and complements (C3 and C4). It could also increase the activity of peritoneal macrophages in mice and the expression of CD4+/CD8+ in the spleen. ELISA analysis showed that the contents of TNF-α, IL-1β and IL-6 were significantly decreased and the content of IL-10 was significantly increased in the PSP group. The western blot analysis showed that PSP could upregulate the relative protein expressions of MUC5AC, PI3K, p-Akt, Rheb and mTOR. These results indicate that PSP can activate the PI3K/Akt/Rheb/mTOR signaling pathway to improve the immunity of mice and maintain the balance of the immune system, thereby protecting the gastric mucosa and improving stress gastric ulcers.

Alginate raft as carrier for self-emulsifying drug delivery system of curcuminoid: In vitro and in vivo analysis

Hemorrhagic gastric ulcer can cause chronic inflammation that can lead to gastro-intestinal perforation and gastric adenocarcinoma or other serious gastric complications. Among naturally occurring treatments, curcumin (CuR) was used but it has limitations of low solubility and rapid first pass effect due to presence of diketo moiety. The present study aimed, to synthesize new synthetic derivative of CuR named curcuminoid (CuRD) and evaluate for its biological activity against gastric ulcer. Solubility and permeation of CuRD were enhanced by development of self-emulsifying drug delivery system (SEDDS). Pseudo-ternary phase diagram was constructed to identify the most efficient self-emulsification region. Optimized CuRD-SEDDS were further employed in the preparation of R-CuRD-SEDDS for local drug delivery in the stomach and all quality control test, raft resilience and strength, raft weight and volume, in-vitro dissolution, anti-oxidant and in-vitro anti-inflammatory activity were also observed. R-CuRD-SEDDS possess significant 20-fold increase in anti-oxidant and 10-fold increase in anti-inflammatory activities as compared to naturally occurring CuR. 70–80 % release within 1 hr, 85 % ulcer inhibition and 3.02 ± 1.5 ulcer index in aspirin induced ulcer was found. Newly developed R-CuRD-SEDDS with enhanced anti-oxidant and anti-inflammatory activities was an alternative approach for the treatment of gastric ulcer.

Effectiveness of natural biomaterials in the protection and healing of experimentally induced gastric mucosa Ulcer in rats

BackgroundA gastric ulcer is a painful lesion of the gastric mucosa that can be debilitating or even fatal. The effectiveness of several plant extracts in the therapy of this illness has been demonstrated in traditional pharmacopoeias. Aim: this study was aimed to see if propolis, ginseng in normal or nano form, and amygdalin might help in preventing the ulcerative effects of absolute ethanol.MethodsGastroprotective properties of pretreatments before ethanol gavage in rats were compared to omeprazole. The ulcer and stomach parameters (ulcerated regions) were measured (mm2), ulcer inhibition percentage, the stomachs were assessed macroscopically with gastric biopsy histological examinations.ResultsAmygdalin, normal and nano ginseng, nano propolis followed by propolis all showed great efficacy in protecting the cyto-architecture and function of the gastric mucosa. The number of ulcerated sites was greatly reduced, and the percentage of stomach protection was increased. Histopathological examination had confirmed great protective effects of the nanoformulations followed by amygdalin. The protection and healing rate was completed to about 100% in all tested materials while ulcer areas were still partially unhealed in normal propolis and omeprazole. Quantitative assay of the m-RNA levels Enothelin 1(ET-1), leukotriene4 (LT-4), and caspase 3(Cas-3) genes and Histamine were done and revealed significant up-regulations in ethanol group and the maximum protective effect was reported with ginseng nano, moreover the histamine content was significantly decreased with nano- formulated extracts.ConclusionAmygdalin and the nanoformulated ginseng and propolis had exhibited a marked protective effect against the ulcerative toxic effects of ethanol.

The effects of alpha-pinene on the Nrf2-HO1 signaling pathway in gastric damage in rats.

Alpha-pinene (α-pinene) is a monoterpene with gastroprotective activity. We evaluated the gastroprotective effect of α-pinene in the gastric damage model with ethanol. We evaluated the macroscopic evaluation of the stomach cavity, alteration in pH, mRNA expression of nuclear factor erythroid 2- related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), total antioxidant capacity (TAC) levels, and histopathological changes. Pretreatment with α-pinene (10, 50 and 100 mg/kg i.p.) before oral administration of ethanol reduced gastric mucosal damage by increasing the percentage of ulcer inhibition. Alpha-pinene also increased gastric pH similar to omeprazole. In addition, the histopathological examination showed that in the groups pretreated with α-pinene 50 and 100 mg/kg, and omeprazole20 mg/kg, the lesions were less than the control group. Moreover, α- pinene 10, 50, 100, and omeprazole 20 mg/kg upregulated the NRF2 and HO1. Our results show that pretreatment with α-pinene is effective in reducing ethanol-induced gastric damage through regulation of Nrf2/HO-1.

Characterization and gastroprotective effects of Rosa brunonii Lindl. fruit on gastric mucosal injury in experimental rats - A preliminary study.

Gastric ulcer is the most prevalent disorder affecting a large population. Rosa brunonii Lindl. fruit (RBF) has traditionally been used to treat stomach pains. Therefore, the current work aimed to isolate, characterize, and investigate the gastro-protective effect of Rosa brunonii Lindl. fruit chloroform extract (RBFCE) against ethanol-induced gastric ulcers in rats. Quercetin 3-O-glucoside (QUE-G) was isolated and characterized by modern spectroscopic techniques. RBFCE was orally administered at 250 mg/kg, 500 mg/kg, and 750 mg/kg doses for ten days. Gastric ulcer was induced by a single dose of absolute ethanol (5 ml/kg) on the last day of the study. Histological changes were calculated, along with ulcer inhibition and the ulcer index (UI). Gastric juice volume, pH, acidity, mucus content, and protein content were evaluated to understand the mechanism underlying its gastroprotective effect. Omeprazole (OMP) was used as the positive control. RBFCE at a dose of 750 mg/kg significantly (p<0.01) reduced the UI (3.54) and increased the protection rate (67.63%) compared to the negative (ulcer) control group. Treatment with RBFCE in a dose-dependent manner increased the gastric pH, mucus content, and total protein while decreasing gastric juice volume and total acidity. Histopathological studies showed severe gastric mucosal injury and edema in ulcer control animals compared to extract-treated groups. This study demonstrated that oral administration of RBFCE possesses a significant gastroprotective effect due to its anti-secretory and cytoprotective mechanisms. Our findings support the traditional use of RBF to treat the gastric ulcer.

Therapeutic effect and mechanism of non-polysaccharide fraction of Bletillae Rhizoma in treatment of gastric ulcer based on network pharmacology and animal experiment

The present study aimed to explore the therapeutic effect and mechanism of non-polysaccharide fraction of Bletillae Rhizoma in the treatment of gastric ulcer by network pharmacology and animal experiments. UPLC-Q-TOF-MS/MS was employed to chara-cterize the chemical components of non-polysaccharide fraction of Bletillae Rhizoma, and the common targets of Bletillae Rhizoma and gastric ulcer were screened out by network pharmacology. The &quot;drug-component-target-disease&quot; network was constructed. Protein-protein interaction(PPI) network was established by STRING. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses were performed based on Matescape database to predict the therapeutic effect and mechanism of Bletillae Rhizoma. Finally, the gastric ulcer model was induced in mice by alcohol to verify the therapeutic effect and mechanism of non-polysaccharide fraction of Bletillae Rhizoma on gastric ulcer. Forty-seven chemical components were identified from non-polysaccharide fraction of Bletillae Rhizoma, among which gymnoside Ⅰ, gymnoside Ⅱ, militarine, bletilloside A, and shancigusin I might be the main active components of non-polysaccharide fraction of Bletillae Rhizoma against gastric ulcer. PPI network analysis revealed core targets such as albumin(ALB), serine/threonine kinase 1(AKT1), tumor necrosis factor(TNF), and epidermal growth factor receptor(EGFR). The KEGG enrichment analysis showed that non-polysaccharide fraction of Bletillae Rhizoma mainly exerted the therapeutic effect by regulating the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT) signaling pathway, mitogen-activated protein kinase(MAPK) signaling pathway, and Ras signaling pathway. The results of animal experiments showed that non-polysaccharide fraction of Bletillae Rhizoma could significantly improve alcohol-induced ulceration in mice to increase ulcer inhibition rate, decrease the levels of TNF-α, interleukin(IL)-1β, IL-6, vasoactive intestinal peptide(VIP), and thromboxane B2(TXB2), elevated the le-vels of IL-10, prostaglandin E2(PGE2), epidermal growth factor(EGF), and vascular endothelial growth factor(VEGF), down-re-gulate the protein levels of PI3K and AKT, and up-regulate the protein levels of p-PI3K and p-AKT. This study indicates that Bletillae Rhizoma may play a role in the treatment of gastric ulcer through multiple components, targets, and pathways and verifies partial prediction results of network pharmacology. The findings of this study provide a scientific and experimental basis for clinical application.

GASTROPROTECTIVE ACTIVITY OF ETHYL ACETATE FRACTION OBTAINED FROM THE STEM BARK EXTRACT OF DESERT DATE (BALANITES AEGYPTIACA)

The study aimed to investigate the gastroprotective activity of the ethyl acetate fraction derived from the stem bark extract of the Desert date tree (EFDD) (Balanites aegyptiaca L. Delile; family Zygophyllaceae) using ethanol and indomethacin-induced ulcer models in Wistar rats. The study was conducted using Wistar rats and divided them into five groups (n=5). Group 1 received 10% Tween 20 (1 ml/kg), which served as the control group. Group 2 was administered the standard drug, omeprazole (20 mg/kg). Groups 3-5 were the extract groups and received doses of 125, 250, and 500 mg/kg, respectively, of EFDD. Two ulcer models were used in the study namely ethanol-induced ulcers and indomethacin-induced ulcers. After administration of the respective treatments, evaluation of the mean ulcer indices in each group and calculation of the percentage ulcer inhibition compared to the control group were carried out. The results indicated that the EFDD, at doses of 250 and 500 mg/kg, significantly reduced the mean ulcer indices in both the ethanol and indomethacin-induced ulcer models compared to the control group (p&lt;0.05). This suggests that the EFDD possesses gastroprotective properties. In conclusion, the EFDD exhibits gastroprotective activity. This finding supports the traditional use of Desert date in folkloric medicine for the treatment of ulcers. However, further research is necessary to explore the underlying mechanisms responsible for the observed gastroprotective effects.

Atractylodes Processing Products Protect against Gastric Ulcers in Rats by Influencing the NF-κB-MMP-9/TIMP-1 Regulatory Mechanism and Intestinal Flora.

Atractylodes macrocephala Koidz. (AM) is a Chinese herbal medicine that is widely used for treating gastrointestinal diseases. However, little research has focused on it as a single medicine for treating gastric ulcers. Honey-bran stir-frying is a characteristic method of concocting AM, so we speculated that AM is more effective after this preparation process. Analysis by ultra-high-performance liquid chromatography-hybrid quadrupole-Orbitrap high-resolution mass spectrometry revealed changes in the chemical composition of raw Atractylodes (SG), bran-fried Atractylodes (FG), and honey-bran-fried Atractylodes (MFG). MFG was superior to SG and FG in improving the pathological structure of gastric tissue in rats with acute gastric ulcers, reducing inflammatory cell infiltration in gastric tissue, and significantly reducing malondialdehyde while increasing superoxide dismutase and glutathione peroxidase, and reducing the damage caused by free radical accumulation in the gastric mucosa. In addition, MFG reduced the expression of matrix metalloproteinase-9 (MMP-9), an inhibitor of metalloproteinase-1 (TIMP-1) and nuclear factor kappa-B (NF-κB)proteins, inhibited inflammatory response, and regulated the degradation and rebalancing of the extracellular matrix. Fecal microbiota analysis also revealed that MFG normalized the intestinal flora to some extent. Our study shows that AM had a protective effect on rats with alcohol-induced acute gastric ulcers before and after processing, and AM-processed products were more effective than raw ones. Compared with MF, MFG had a higher rate of ulcer inhibition and a stronger anti-inflammatory effect, and its mechanism of action was related to the NF-κB-MMP-9/TIMP-1 signaling pathway.

Investigation Into Antioxidant and Antinuclear Effect and Mechanism of Sesamum Indicum Leaves in a Rodent Model

Objective: An ethanolic extract of Sesamum indicum Linn was used to test for phytochemicals, antioxidant activity, and gastroprotective effects. Study Design: Cross-Sectional Study Place of Study: Rashid Latif Medical College Lahore. Duration of Study: July 2021 to March 2022 Materials and methods: This study ulcerated Wister albino rats using pylorus ligation and indomethacin-induced ulcer screening. Ulceration required pylorus ligation. These ulcer screening models were used. An ethanolic S. indicum leaves (EESIL) at 100, 200, and 400 mg/kg (orally for 7 days) was compared against omeprazole, a common ulcer medication. The trial lasted seven days. Throughout the course of the experiment, readings were taken for the following variables: stomach content; pH; total acidity; pepsin activity ulcer score; free acidity; ulcer index (UI); percentage of inhibition of ulcers; mean mucin, pepsin, and total protein content; and ulcer index (UI). Results: In comparison to the control group, the pylorus ligation model resulted in a decrease in pepsin activity, free acidity, pepsin content, ulcer score, total protein content, and % ulcer inhibition. Moreover, the overall acidity level was lower (P&lt; 0.05 and P&lt; 0.01). EESIL-tested groups had higher mean mucin and stomach content pH (P 0.05 and P 0.01). Both findings are statistically significant. EESIL dosages (100, 200, and 400 mg/kg ) had dose-dependent gastroprotective effects. Compared to the control group, stomach metrics like UI and ulcer score dropped significantly (P&lt; 0.05 and P&lt;0.01), gastric pH increased, and ulcer percentage inhibition increased. Increased ulcer inhibition percentage. Moreover, stomach pH increased and ulcer percentage decreased. Conclusion: Antioxidant, anti-ulcer, EESIL, and EESIL compounds have antioxidant action depending on concentration. Antioxidant is connected. The study found that the EESIL's increased defensive and decreased offensive components increased its antiulcer potential. This was due to the study's revisions. The research's offensive and defensive design caused this. Keywords: Antioxidant, anti-ulcer, ethanolic extract of sesamum indicum leaves, indomethacin, pylorus ligation, ulcer index

The case for the therapeutic use of mechanistic/mammalian target of rapamycin (mTOR) inhibitors in xenotransplantation.

The mechanistic/mammalian target of rapamycin (mTOR) is one of the systems that are necessary to maintain cell homeostasis, such as survival, proliferation, and differentiation. mTOR inhibitors (mTOR-Is) are utilized as immunosuppressants and anti-cancer drugs. In organ allotransplantation, current regimens infrequently include an mTOR-I, which are positioned more commonly as alternative immunosuppressants. In clinical allotransplantation, long-term efficacy has been established, but there is a significant incidence of adverse events, for example, inhibition of wound healing, buccal ulceration, anemia, hyperglycemia, dyslipidemia, and thrombocytopenia, some of which are dose-dependent. mTOR-Is have properties that may be especially beneficial in xenotransplantation. These include suppression of T cell proliferation, increases in the number of T regulatory cells, inhibition of pig graft growth, and anti-inflammatory, anti-viral, and anti-cancer effects. We here review the potential benefits and risks of mTOR-Is in xenotransplantation and suggest that the benefits exceed the adverse effects.

Effect of Flavoring with Rosemary, Lemon and Orange on the Quality, Composition and Biological Properties of Olive Oil: Comparative Study of Extraction Processes

The goal of this work was to investigate the impact of the flavoring of some aromatic plants/spices, including rosemary (R), lemon (L) and orange (O) at the concentration of 5% and 35% (w/w) added by 2 methods (conventional maceration and direct flavoring), on quality attributes, chemical changes and oxidative stability of extra virgin olive oil (EVOO). Six flavored oils were obtained (EVOO + O, O + O, EVOO + R, O + R, EVOO + L and O + L). The physicochemical parameters (water content, refractive index, acidity and peroxide value, extinction coefficient, fatty acids, volatile aroma profiles, Rancimat test, phenols and pigments composition) of the flavored oils were investigated. Based on the results obtained, it was observed that flavoring with a conventional process provided increased oxidative stability to the flavored oils, especially with rosemary (19.38 ± 0.26 h), compared to that of unflavored oil. The volatile profiles of the different flavored oils revealed the presence of 34 compounds with the dominance of Limonene. The fatty acid composition showed an abundance of mono-unsaturated fatty acids followed by poly-unsaturated ones. Moreover, a high antioxidant activity, a significant peripheral analgesic effect (77.7% of writhing inhibition) and an interesting gastroprotective action (96.59% of ulcer inhibition) have been observed for the rosemary-flavored oil. Indeed, the flavored olive oils of this study could be used as new functional foods, leading to new customers and further markets.