Potential Inhalation Exposure Research Articles

Entomological assessment of hessian fabric transfluthrin vapour emanators for protecting against outdoor-biting Aedes aegypti in coastal Tanzania.

A low technology emanator device for slowly releasing vapour of the volatile pyrethroid transfluthrin was recently developed in Tanzania that provides robust protection against night biting Anopheles and Culex vectors of malaria and filariasis for several months. Here these same emanator devices were assessed in Dar es Salaam city, as a means of protection against outdoor-biting Aedes (Stegomia) aegypti, the most important vector of human arboviruses worldwide, in parallel with similar studies in Haiti and Brazil. A series of entomological experiments were conducted under field and semi-field conditions, to evaluate whether transfluthrin emanators protect against wild Ae. aegypti, and also compare the transfluthrin responsiveness of Ae. aegypti originating from wild-caught eggs to established pyrethroid-susceptible Ae. aegypti and Anopheles gambiae colonies. Preliminary measurements of transfluthrin vapour concentration in air samples collected near treated emanators were conducted by gas chromatography-mass spectrometry. Two full field experiments with four different emanator designs and three different transfluthrin formulations consistently indicated negligible reduction of human landing rates by wild Ae. aegypti. Under semi-field conditions in large cages, 50 to 60% reductions of landing rates were observed, regardless of which transfluthrin dose, capture method, emanator placement position, or source of mosquitoes (mildly pyrethroid resistant wild caught Ae. aegypti or pyrethroid-susceptible colonies of Ae. aegypti and An. gambiae) was used. Air samples collected immediately downwind from an emanator treated with the highest transfluthrin dose (15g), contained 12 to 19 μg/m3 transfluthrin vapour. It appears unlikely that the moderate levels of pyrethroid resistance observed in wild Ae. aegypti can explain the modest-to-undetectable levels of protection exhibited. While potential inhalation exposure could be of concern for the highest (15g) dose evaluated, 3g of transfluthrin appears sufficient to achieve the modest levels of protection that were demonstrated entomologically. While the generally low levels of protection against Aedes reported here from Tanzania, and from similar entomological studies in Haiti and Brazil, are discouraging, complementary social science studies in Haiti and Brazil suggest end-users perceive valuable levels of protection against mosquitoes. It therefore remains unclear whether transfluthrin emanators have potential for protecting against Aedes vectors of important human arboviruses.

Novel field data for exposure of bystanders and residents towards spray drift during application of plant protection products in orchards

Exposure to pesticide spray drift during the application of plant protection products (PPP) in orchards poses potential health risks to bystanders and residents. To address this concern, this study aimed to gather novel field data on exposure to spray drift in orchards. The research was conducted by the Julius Kuehn-Institute (JKI), the Federal Office of Consumer Protection and Food Safety (BVL), and the German Federal Institute for Risk Assessment (BfR). From 2018 to 2021, the method for conducting the field trials and analysis was implemented. The data from multiple series of performed field trials conducted between 2021 and 2022 allowed for collecting data on potential dermal exposure (PDE), inhalation exposure, and ground sediment. The trials considered various distances from the treatment area and drift-reducing techniques. Adult and child mannequins were used to simulate bystanders’ exposure, and petri dishes were used to measure ground sediment. The results showed that drift reduction settings (75% drift reduction) considerably reduced exposure levels on the mannequins. Furthermore, a strong correlation between PDE values for adults and children located next to each other at the same distance was observed. This finding suggests a possibility to convert exposure results from adult to child mannequins. The study produced realistic exposure data for bystanders and residents next to a treated area. It contributes to a better understanding of exposure risks from PPP application and includes data for different distances and spray drift reducing technique. If taken into account for further development of the current risk assessment models, new risk management options for the safe application of PPP, including buffer strips or application of drift reducing systems, might be available in near future.

Effect of nebuliser and patient interface type on fugitive medical aerosol emissions in adult and paediatric patients

BackgroundOpen circuit aerosol therapy is associated with the potential for fugitive emissions of medical aerosol. Various nebulisers and interfaces are used in respiratory treatments, including the recent consideration of filtered interfaces. This study aims to quantify fugitive medical aerosols from various nebuliser types, in conjunction with different filtered and non-filtered interfaces. MethodsFor both simulated adult and paediatric breathing, four nebuliser types were assessed including; a small volume jet nebuliser (SVN), a breath enhanced jet nebuliser (BEN), a breath actuated jet nebuliser (BAN) and a vibrating mesh nebuliser (VMN). A combination of different interfaces were used including filtered and unfiltered mouthpieces, as well as open, valved and filtered facemasks. Aerosol mass concentrations were measured using an Aerodynamic Particle Sizer at 0.8 m and 2.0 m. Additionally, inhaled dose was assessed. ResultsHighest mass concentrations recorded were 214 (177, 262) µg m−3 at 0.8 m over 45-minute run. The highest and lowest fugitive emissions were observed for the adult SVN facemask combination, and the adult BAN filtered mouthpiece combination respectively. Fugitive emissions decreased when using breath-actuated (BA) mode compared to continuous (CN) mode on the BAN for the adult and paediatric mouthpiece combination. Lower fugitive emissions were observed when a filtered facemask or mouthpiece was used, compared to unfiltered scenarios. For the simulated adult, highest and lowest inhaled dose were 45.1 (42.6, 45.6)% and 11.0 (10.1,11.9)% for the VMN and SVN respectively. For the simulated paediatric, highest and lowest inhaled dose were 44.0 (42.4, 44.8)% and 6.1 (5.9, 7.0)% for the VMN and BAN CN respectively. Potential inhalation exposure of albuterol was calculated to be up to 0.11 µg and 0.12 µg for a bystander and healthcare worker respectively. ConclusionThis work demonstrates the need for filtered interfaces in clinical and homecare settings to minimise fugitive emissions and to reduce the risk of secondary exposure to care givers.

Identification of molecular initiating events (MIE) using chemical database analysis and nuclear receptor activity assays for screening potential inhalation toxicants

An adverse outcome pathway (AOP) framework can facilitate the use of alternative assays in chemical regulations by providing scientific evidence. Previously, an AOP, peroxisome proliferative-activating receptor gamma (PPARγ) antagonism that leads to pulmonary fibrosis, was developed. Based on a literature search, PPARγ inactivation has been proposed as a molecular initiating event (MIE). In addition, a list of candidate chemicals that could be used in the experimental validation was proposed using toxicity database and deep learning models. In this study, the screening of environmental chemicals for MIE was conducted using in silico and in vitro tests to maximize the applicability of this AOP for screening inhalation toxicants. Initially, potential inhalation exposure chemicals that are active in three or more key events were selected, and in silico molecular docking was performed. Among the chemicals with low binding energy to PPARγ, nine chemicals were selected for validation of the AOP using in vitro PPARγ activity assay. As a result, rotenone, triorthocresyl phosphate, and castor oil were proposed as PPARγ antagonists and stressor chemicals of the AOP. Overall, the proposed tiered approach of the database-in silico-in vitro can help identify the regulatory applicability and assist in the development and experimental validation of AOP.

Heavy metal contamination levels, source distribution, and risk assessment in fine sand of urban surface deposited sediments of Ekaterinburg, Russia.

Urban surface deposited sediments (USDS) are unique indicators of local pollution that pose a potential threat to the living environment and human health. Ekaterinburg is a highly populated metropolitan area in Russia with rapid urbanization and industrialization activities. In Ekaterinburg's residential areas, about 35, 12, and 16 samples are represented by green zones, roads, driveways, and sidewalks, respectively. The total concentrations of heavy metals was detected using a chemical analyzer inductively coupled plasma mass spectrometry (ICP-MS). Zn, Sn, Sb, and Pb have the highest concentrations in the green zone, while V, Fe, Co, and Cu represent the utmost values on roads. Moreover, Mn and Ni are the prevailing metals in the fine sand fraction of driveways along with sidewalks. Broadly, the high pollution in the studied zones is generated by anthropogenic activities and traffic emissions. The potential ecological risk (RI) was observed in high risk (IR > 600), even though the results of all heavy metals reveal no adverse health effects from the considered noncarcinogenic metal for adults and children by different exposure pathways except the children's exposure to Co in case of the dermal contact, where the HI values of Co for children in the studied zones are higher than the proposed level (> 1). In all urban zones, the total carcinogenic risk (TLCR) values are predicted as a high potential inhalation exposure.

Potentially harmful elements in urban surface deposited sediment of Ekaterinburg, Russia: Occurrence, source appointment and risk assessment

In this study, the human health risks of potentially harmful elements (PHEs) in urban surface deposited sediments (USDS) were examined by collecting urban dust samples, measuring their PHE concentrations, and using index evaluation. About 35, 12 and 16 samples are represented a green zones, roads, driveways and sidewalks in residential areas of Ekaterinburg, respectively. The dust fraction (0.002–0.1 mm) was obtained by sieving, filtration, and decantation process. Total concentrations of 10 PHEs were measured using inductively coupled plasma mass spectrometry. The highest concentrations of Pb were found in USDS from green zones, while Fe, V, Mn, Co, Ni, Sn, and Sb on roads, Cu and Zn on driveways and sidewalks. The contamination levels in the investigated land-use areas were studied, where the highest contamination was contributed from Sb in the driveways and sidewalk. Moreover, the pollution in the studied zones was a high load, contributing to anthropogenic activities and traffic emissions. No non-cancerogenic risk was attributed from the PHEs based on the results of health indices (HI < 1) for both adult and children, except Co and Ni which has HI > 1 for children. The total carcinogenic risk (TLCR) in all urban landscape areas is defined as a high potential inhalation exposure and a low potential ingestion and dermal exposure.

Characteristics and Potential Inhalation Exposure Risks of Environmentally Persistent Free Radicals in Atmospheric Particulate Matter and Solid Fuel Combustion Particles in High Lung Cancer Incidence Area, China

Environmentally persistent free radicals (EPFRs) were previously considered an unrecognized composition of air pollutants and might help explain the long-standing medical mystery of why non-smokers develop tobacco-related diseases such as lung cancer. However, there is no investigated on EPFRs in Xuanwei rural areas, especially in high prevalence of lung cancer areas. In this study, we selected six types of coal and three types of biomass in Xuanwei, then conducted simulated combustion, and six group of atmospheric particulate matters (APMs) to explore the content and particle size distribution pattern of EPFRs and a new health risk assessment method to evaluate the risk of EPFRs in PM for adults and children. Our results show that the contribution of EPFRs for biomass combustion, coal combustion and APMs were mainly distributed in the size range of &lt;1.1 μm, which accounted for 76.15 ± 4.14%, 74.85 ± 10.76%, and 75.23 ± 8.18% of PM3.3. The mean g factors and ΔHp-p indicated that the EPFRs were mainly oxygen-centered radicals in PM in Xuanwei. The results suggest that the health risk of EPFRs is significantly increased when the particle size distribution of EPFRs is taken into account, and coal combustion particulate matter (174.70 ± 37.86 cigarettes for an adult, 66.39 ± 14.39 cigarettes per person per year for a child) is more hazardous to humans than biomass combustion particulate matter (69.41 ± 4.83 cigarettes for an adult, 26.37 ± 1.84 cigarettes per person per year for), followed by APMs (102.88 ± 39.99 cigarettes for an adult, 39.10 ±15.20 cigarettes per person per year for) in PM3.3. Our results provides a new perspective and evidence for revealing the reason for the high incidence of lung cancer in Xuanwei, China.

Potential human inhalation exposure to soil contaminants in urban gardens on brownfields sites: A breath of fresh air?

Urban gardening has been experiencing increased popularity around the world. Many urban gardens are located on sites that may be contaminated by trace elements or organic compounds due to previous use. The three main exposure pathways to the human body for soil contaminants are (a) ingestion of soil directly, (b) consumption of produce containing or superficially contaminated with a contaminant, (c) and inhalation of soil dust. The first two modes have received much attention; however, the contribution of the inhalation route has not been investigated adequately. Two inhalation risk studies were carried out in urban gardens located in Kansas City, MO, by collecting dust while 25-m2 plots were rototilled. Microclimatic variables were monitored, and total inhalable dust mass was determined using a personal sampling train including a small pump and air filter. Soil lead (Pb) concentration was assessed at both sites. For Study 1, particle size distribution of collected particles was estimated through analysis of scanning electron microscope images of filters. Little dust was collected at either site. Most particles captured, however, appeared to be <4μm in diameter. The amount of dust emitted was correlated with soil moisture. Tilling reduced soil aggregate size and blended soil, resulting in a more homogeneous distribution of Pb. Dust inhalation while tilling is likely not a major Pb exposure risk for gardeners, but given the preponderance of very small particles in what was captured, care should be taken to prevent dust from entering the respiratory system.

3D printer particle emissions: Translation to internal dose in adults and children

Desktop fused deposition modeling (FDM®) three-dimensional (3D) printers are becoming increasingly popular in schools, libraries, and among home hobbyists. FDM® 3D printers have been shown to release ultrafine airborne particles in large amounts, indicating the potential for inhalation exposure and consequent health risks among FDM® 3D printer users and other room occupants including children. These particles are generated from the heating of thermoplastic polymer feedstocks during the FDM® 3D printing process, with the most commonly used polymers being acrylonitrile butadiene styrene (ABS) and poly-lactic acid (PLA). Risk assessment of these exposures demands estimation of internal dose, especially to address intra-human variability across life stages. Dosimetry models have proven to effectively translate particle exposures to internal dose metrics relevant to evaluation of their effects in the respiratory tract. We used the open-access multiple path particle dosimetry (MPPD v3.04) model to estimate inhaled particle deposition in different regions of the respiratory tract for children of various age groups from three months to eighteen years old adults. Mass concentration data for input into the MPPD model were calculated using particle size distribution and density data from experimental FDM® 3D printer emissions tests using both ABS and PLA. The impact of changes in critical parameters that are principal determinants of inhaled dose, including: sex, age, and exposure duration, was examined using input parameter values available from the International Commission on Radiological Protection. Internal dose metrics used included regional mass deposition, mass deposition normalized by pulmonary surface area, surface area of deposited particles by pulmonary surface area, and retained regional mass. Total mass deposition was found to be highest in the 9-year-old to 18-year-old age groups with mass deposition by pulmonary surface area highest in 3-month-olds to 9-year-olds and surface area of deposited particles by pulmonary surface area to be highest in 9-year-olds. Clearance modeling revealed that frequent 3D printer users are at risk for an increased cumulative retained dose.

Risk assessment of imidacloprid and dichlorvos associated with dermal and inhalation exposure in cucumber greenhouse applicators: A cross-sectional study in Hamadan, Iran

ABSTRACT A complex mixture of organic pollutants (EOPs) enters the environment, which has potential impacts on aquatic life and human health.This cross-sectional study investigated dermal and inhalation exposure to the pesticides imidacloprid and dichlorvos and evaluated the health risk in cucumber greenhouses applicators in the Amzajerdagriculturalarea, Hamadan Province, western Iran. A patch method and a personal air sampler with XAD-2 absorbent were used to measure potential exposure. In mixing and loading, dermal exposure on the hands was 1.15 mg for imidacloprid and 2.27 mg for dichlorvos. During the application of imidacloprid and dichlorvos, total dermal exposure was 8.49 and 16.21 mg, respectively. The calculated absorbable quantities of exposure (AQE) during mixing/loading were 0.0017 mg/day for imidacloprid and 0.0054 mg/day for dichlorvos, whereas AQE during application were 0.159 and 0.437 mg/day, respectively. The arms were the most contaminated anatomical regions of the applicators. The potential inhalation exposure contributed to <7% of the total exposure for both pesticides. For risk assessment, the margin of safety (MOS) was calculated. The MOS for imidacloprid was higher than 1, indicating that the possible risk associated with imidacloprid was low, whereas the MOS for dichlorvos was <1, which indicates a potential risk to the human body that merits concern from policy makers in pest management.

Safety of vitamin B12 (in the form of cyanocobalamin) produced by Ensifer adhaerensCNCM-I 5541 for all animal species.

The vitamin B12 (in the form of cyanocobalamin) under assessment is produced by fermentation with a genetically modified strain of Ensifer adhaerens and it is intended to be used as a nutritional additive for all animal species. In 2018, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) of EFSA issued an opinion on the safety and efficacy of the product. In that assessment, the Panel could not conclude on the safety of the additive for the target species, consumers and the environment due to uncertainties on the safety of the production strain and the resulting product. Due to high endotoxin content, potential inhalation exposure when handling premixtures and reported irritancy for skin and eyes, the additive was considered to pose a risk to user safety. The applicant provided supplementary data on the identity of the production strain, its susceptibility to antibiotics and toxigenic potential, as well as on the absence of cells and recombinant DNA of the production strain in the final product. The production strain is not expected to produce any toxic compound during fermentation but harbours antimicrobial resistance genes. However, viable cells and recombinant DNA of the strain were not detected in the most concentrated form of the additive. With this new information, the FEEDAP Panel concluded that vitamin B12 produced by E. adhaerens CNCM I‐5541 (identified as SCM 2034 in the previous opinion) is safe for all animal species, the consumers and the environment. The applicant did not provide new evidence that would lead the FEEDAP Panel to reconsider previous conclusions regarding the safety for the user.

Potential consumer exposure to respirable particles and TiO2 due to the use of eyebrow powders.

BackgroundCosmetic powders contain numerous components, including titanium dioxide (TiO2), which is classified as possibly carcinogenic to humans (Group 2B). However, little is known about potential inhalation exposures to particles that are released during cosmetic powder applications.MethodsWe realistically simulated the application of five different eyebrow powders using a mannequin and then determined concentrations of total suspended particles (TSP), PM10, and PM4 fractions of particles that would be inhaled during powder application. We determined the size and shape of particles in the original powders and released particles, as well as their TiO2 concentrations and Ti content of individual particles.ResultsThe application of eyebrow powders resulted in the release and inhalation of airborne particles at concentrations ranging from 21.2 to 277.3 μg/m3, depending on the particle fraction and the powder. The concentrations of TiO2 in PM4 and PM10 samples reached 2.7 μg/m3 and 9.3 μg/m3, respectively. The concentration of TiO2 in airborne particle fractions was proportional to the presence of TiO2 in the bulk powder.ConclusionThe application of eyebrow powders results in user exposures to respirable PM4 and PM10 particles, including those containing TiO2. This information should be of interest to stakeholders concerned about inhalation exposure to TiO2.

It Is Time to Address Airborne Transmission of Coronavirus Disease 2019 (COVID-19).

The following scientists reviewed the document: Jonathan Abbatt, John Adgate, Alireza Afshari, KangHo Ahn, Francis Allard, Joseph Allen, Celia Alves, Meinrat O. Andreae, Isabella Annesi-Maesano, Ahmet Arisoy, Andrew P. Ault, Gwi-Nam Bae, Gabriel Beko, Scott C. Bell, Allan Bertram, Mahmood Bhutta, Seweryn Bialasiewicz, Merete Bilde, Tami Bond, Joseph Brain, Marianna Brodach, David M. Broday, Guangyu Cao, Christopher D. Cappa, Annmarie Carlton, Paul K. S. Chan, Christopher Chao, Kuan-Fu Chen, Qi Chen, Qingyan Chen, David Cheong, Per Axcel Clausen, Ross Crawford, Derek Clements-Croome, Geo Clausen, Ian Clifton, Richard L. Corsi, Benjamin J. Cowling, Francesca Romana d'Ambrosio, Ghassan Dbaibo, Richard de Dear, Gianluigi de Gennaro, Peter DeCarlo, Philip Demokritou, Hugo Destaillats, Joanna Domagala-Kulawik, Neil M. Donahue, Caroline Duchaine, Marzenna R. Dudzinska, Dominic E. Dwyer, Greg Evans, Delphine K. Farmer, Kevin P. Fennelly, Richard Flagan, Janine Frohlich-Nowoisky, Manuel Gameiro da Silva, Christian George, Marianne Glasius, Allen H. Goldstein, Joao Gomes, Michael Gormley, Rafal Gorny, David Grimsrud, Keith Grimwood, Charles N. Haas, Fariborz Haghighat, Michael Hannigan, Roy Harrison, Ulla HaverinenShaughnessy, Philippa Howden-Chapman, Per Heiselberg, Daven K. Henze, Jean-Michel Heraud, Hartmut Herrmann, Philip K. Hopke, Ray Horstman, Wei Huang, Alex Huffman, David S. Hui, Tareq Hussein, Gabriel Isaacman-VanWertz, Jouni J.K. Jaakkola, Matti Jantunen, Lance Jennings, Dennis Johansson, Jan Kaczmarczyk, George Kallos, David Katoshevski, Frank Kelly, Soren Kjaergaard, Luke D. Knibbs, Henrik N. Knudsen, GwangPyo Ko, Evelyn S.C. Koay, Jen Kok, Nino Kuenzli, Markku Kulmala, Kazukiyo Kumagai, Prashant Kumar, Kazumichi Kuroda, Kiyoung Lee, Nelson Lee, Barry Lefer, Vincent Lemort, Xianting Li, Dusan Licina, Chao-Hsin Lin, Junjie Liu, Kam Lun E. Hon, John C. Little, Li Liu, Janet M. Macher, Ebba Malmqvist, Corinne Mandin, Ivo Martinac, Dainius Martuzevicius, Mark J. Mendell, David Miller, Claudia Mohr, Luisa T. Molina, Glenn Morrison, Roya Mortazavi, Edward Nardell, Athanasios Nenes, Mark Nicas, Zhi Ning, Jianlei Niu, Hidekazu Nishimura, Colin O'Dowd, Bjarne W. Olesen, Paula J. Olsiewski, Spyros Pandis, Daniel Peckham, Tuukka Petaja, Zbigniew Popiolek, Ulrich Poschl, Wayne R. Ott, Kimberly Prather, Andre S. H. Prevot, Hua Qian, Shanna Ratnesar-Shumate, James L. Repace, Tiina Reponen, Ilona Riipinen, Susan Roaf, Allen L. Robinson, Yinon Rudich, Manuel Ruiz de Adana, Masayuki Saijo, Reiko Saito, Paulo Saldiva, Tunga Salthammer, Joshua L. Santarpia, John H. Seinfeld, Gary S. Settles, Siegfried Schobesberger, Paul T. J. Scheepers, Max H. Sherman, Alan Shihadeh, Manabu Shiraiwa, Jeffrey Siegel, Torben Sigsgaard, Brett C. Singer, James N. Smith, Armin Sorooshian, Jerzy Sowa, Brent Stephens, Huey-Jen Jenny Su, Jordi Sunyer, Jason D. Surratt, Kazuo Takahashi, Nobuyuki Takegawa, Jorn Toftum, Margaret A. Tolbert, Euan Tovey, Barbara J. Turpin, Annele Virtanen, John Volckens, Claire Wainwright, Lance A. Wallace, Boguang Wang, Chia C. Wang, Michael Waring, John Wenger, Charles J. Weschler, Brent Williams, Mary E. Wilson, Armin Wisthaler, Kazimierz Wojtas, Douglas R. Worsnop, Ying Xu, Naomichi Yamamoto, Xudong Yang, Hui-Ling Yen, Hiroshi Yoshino, Hassan Zaraket, Zhiqiang (John) Zhai, Junfeng (Jim) Zhang, Qi Zhang, Jensen Zhang, Yinping Zhang, Bin Zhao, Tong Zhu.

Inappropriate Use of Risk Assessment in Addressing Health Hazards Posed by Civil Aircraft Cabin Air

Over decades, the airline industry has published considerable documentation on the potential for inhalation exposure to organophosphates and other toxins sourced to engine oil fumes supplied by “bleed air” systems, which are used on most commercial and military aircraft. Case studies on the subsequent development of neurological and other symptoms reported by crewmembers have also been widely documented. However, airline industry reviews tend to define toxicity of engine oil fumes according to a single toxicological endpoint - Organo Phosphate Induced Delayed Neuropathy (OPIDN) – which is acknowledged to be the result of a very high dose of exposure to a specific chemical, Tri-Ortho Cresyl Phosphate (TOCP). Industry reviews typically argue that the low levels of TOCP in aviation engine oils justifies the safety of continuing to use unfiltered engine bleed air to ventilate civil and military aircraft cabins. In fact, this approach ignores the routine presence and toxicity of a complex mixture of OPs in oil fumes supplied by engine bleed air systems and ignores the type of neurological symptoms that crews report (i.e., not OPIDN). The industry approach also ignores the scientific literature on repeated low-dose exposure to OPs over extended periods (particularly relevant for airline crewmembers) and the variable susceptibility of individuals to toxicological damage. This paper reviews the above-mentioned studies and presents the scientific literature that should be considered to make a realistic risk assessment of the hazards of aircraft engine bleed air.

Characteristics and potential inhalation exposure risks of PM2.5–bound environmental persistent free radicals in Nanjing, a mega–city in China

PM2.5–bound toxic elements and organic pollutants have been extensively investigated, while limited information is available for environmental persistent free radicals (EPFRs) associated with PM2.5, which may lead to oxidative stress in the human lung when exposed to PM2.5. In this study, the levels and types of PM2.5–bound EPFRs present in Nanjing, a mega–city in China, were analyzed. PM2.5–bound EPFRs were found to mainly be a mixture of carbon– and oxygen–centered radicals. The concentration of PM2.5–bound EPFRs ranged from 2.78 × 1012 to 1.72 × 1013 spins m−3, with an average value of 7.61 × 1012 spins m−3. The half–life of the PM2.5–bound EPFRs was calculated to be an average of 83.5 days when stored at room temperature, with only weak correlations observed between EPFRs and conventional air pollutants (NO2, O3, CO and PM2.5)/PM2.5–bound transition metals (Cu, Zn, Cr, Mn, V, Cd, and Ni) and significant correlations between EPFRs and SO2/PM2.5–bound Fe. PM2.5–bound EPFRs can induce the formation of reactive oxygen species (ROS) in both water and a H2O2 solution, which are used to simulate lung solution of a healthy person and patient, respectively. Therefore, PM2.5–bound EPFRs can lead to potential oxidative stress in humans. Overall, PM2.5–bound EPFRs show an obvious temporal variation and can pose potential health risks to humans via the induction of ROS in the lung solution.

Evaluation of Second-Hand Exposure to Electronic Cigarette Vaping under a Real Scenario: Measurements of Ultrafine Particle Number Concentration and Size Distribution and Comparison with Traditional Tobacco Smoke.

The present study aims to evaluate the impact of e-cig second-hand aerosol on indoor air quality in terms of ultrafine particles (UFPs) and potential inhalation exposure levels of passive bystanders. E-cig second-hand aerosol characteristics in terms of UFPs number concentration and size distribution exhaled by two volunteers vaping 15 different e-liquids inside a 49 m3 room and comparison with tobacco smoke are discussed. High temporal resolution measurements were performed under natural ventilation conditions to simulate a realistic exposure scenario. Results showed a systematic increase in UFPs number concentration (part cm−3) related to a 20-min vaping session (from 6.56 × 103 to 4.01 × 104 part cm−3), although this was one up to two order of magnitude lower than that produced by one tobacco cigarette consumption (from 1.12 × 105 to 1.46 × 105 part cm−3). E-cig second-hand aerosol size distribution exhibits a bimodal behavior with modes at 10.8 and 29.4 nm in contrast with the unimodal typical size distribution of tobacco smoke with peak mode at 100 nm. In the size range 6–26 nm, particles concentration in e-cig second-hand aerosol were from 2- (Dp = 25.5 nm) to 3800-fold (Dp = 9.31 nm) higher than in tobacco smoke highlighting that particles exhaled by users and potentially inhaled by bystanders are nano-sized with high penetration capacity into human airways.

Identifying organic compounds in exhaled breath aerosol: Non-invasive sampling from respirator surfaces and disposable hospital masks

Exhaled breath aerosol (EBA) is an important non-invasive biological medium for detecting exogenous environmental contaminants and endogenous metabolites present in the pulmonary tract. Currently, EBA is typically captured as a constituent of the mainstream clinical tool referred to as exhaled breath condensate (EBC). This article describes a simpler, completely non-invasive method for collecting EBA directly from different forms of hard-surface plastic respirator masks and disposable hospital paper breathing masks without first collecting EBC. The new EBA methodology bypasses the complex EBC procedures that require specialized collection gear, dry ice or other coolant, in-field sample processing, and refrigerated transport to the laboratory. Herein, mask samples collected from different types of plastic respirators and paper hospital masks worn by volunteers in the laboratory were analyzed using high resolution-liquid chromatography-mass spectrometry (HR-LC-MS) and immunochemistry. The results of immunochemistry analysis revealed that cytokines were collected above background on both plastic respirator surfaces and paper hospital masks, confirming the presence of human biological constituents. Non-targeted HR-LC-MS analyses demonstrated that larger exogenous molecules such as plasticizers, pesticides, and consumer product chemicals as well as endogenous biochemicals, including cytokines and fatty acids were also detected on mask surfaces. These results suggest that mask sampling is a viable technique for EBA collection to assess potential inhalation exposures and endogenous indicators of health state.

Characterization of M4 carbine rifle emissions with three ammunition types

Muzzle emissions from firing an M4 carbine rifle in a semi-enclosed chamber were characterized for an array of compounds to provide quantitative data for future studies on potential inhalation exposure and rangeland contamination. Air emissions were characterized for particulate matter (PM) size distribution, composition, and morphology; carbon monoxide (CO); carbon dioxide (CO2); energetics; metals; polycyclic aromatic hydrocarbons; and methane. Three types of ammunition were used: a “Legacy” (Vietnam-era) round, the common M855 round (no longer fielded), and its variant, an M855 round with added potassium (K)-based salts to reduce muzzle flash. Average CO concentrations up to 1500 ppm significantly exceeded CO2 concentrations. Emitted particles were in the respirable size range with mass median diameters between 0.33 and 0.58 μm. PM emissions were highest from the M855 salt-added ammunition, likely due to incomplete secondary combustion in the muzzle blast caused by scavenging of combustion radicals by the K salt. Copper (Cu) had the highest emitted metal concentration for all three round formulations, likely originating from the Cu jacket on the bullet. Based on a mass balance analysis of each round's formulation, lead (Pb) was completely emitted for all three round types. This work demonstrated methods for characterizing emissions from gun firing which can distinguish between round-specific effects and can be used to initiate studies of inhalation risk and environmental deposition.

Dynamic sequential box modelling of inhalation exposure potential in multi-bed patient ward: Validation and baseline case studies

Macro-models provide a fast simulation tool for exposure assessments and design of control interventions, yet there are concerns about their accuracies. Single-zone based models are known to be inadequate for predicting exposure to near-source emissions, while the complexity of sequential box models (SBM) limits their application to steady-state conditions. However, treating unsteady conditions as steady underestimates peak inhalation exposure potentials, especially near the source. This study employs a sub-configuration validation approach to validate the unsteady SBM, using an R open-source-based numerical solver, for estimating indoor pollutant concentration, exposure and/or infection risks. The validated model is used to assess the performance of ASHRAE S170-2017 baseline specifications for inhalation exposure control in a multi-bed patient ward with air recirculation. In the baseline studies, quanta infective concentration and reference concentration were respectively used as source strength and threshold values for influenza pathogen. Robust design methodology was employed in the experimental design and analysis of the control and noise factors. Results indicate a close relationship between SBM and the sub-configuration validation datasets. Findings also show that concentration gradients exist in SBM with the highest values in the near-field zones. Thus, with SBM, the well-mixed assumption does not necessarily imply equal exposure potentials. Robustness analysis shows that stratum ventilation is three-fold insensitive to the variability in exposure than mixing ventilation. Finally, the results of the case studies revealed that the average inhalation exposure exceeds the influenza reference concentration, thereby suggesting an insufficiency of the baseline conditions to offer protection against inhalation exposure to influenza contagion.

Understanding airborne contaminants produced by different fuel packages during training fires

Fire training may expose firefighters and instructors to hazardous airborne chemicals that vary by the training fuel. We conducted area and personal air sampling during three instructional scenarios per day involving the burning of two types (designated as alpha and bravo) of oriented strand board (OSB), pallet and straw, or the use of simulated smoke, over a period of 5 days. Twenty-four firefighters and ten instructors participated. Firefighters participated in each scenario once (separated by about 48 hr) and instructors supervised three training exercise per scenarios (completed in 1 day). Personal air samples were analyzed for polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOCs), and hydrogen cyanide during live-fire scenarios (excluding simulated smoke). Area air samples were analyzed for acid gases, aldehydes, isocyanates, and VOCs for all scenarios. For the live-fire scenarios, median personal air concentrations of benzene and PAHs exceeded applicable short-term exposure limits and were higher among firefighters than instructors. When comparing results by type of fuel, personal air concentrations of benzene and PAHs were higher for bravo OSB compared to other fuels. Median area air concentrations of aldehydes and isocyanates were also highest during the bravo OSB scenario, while pallet and straw produced the highest median concentrations of certain VOCs and acid gases. These results suggest usage of self-contained breathing apparatus (SCBA) by both instructors and firefighters is essential during training fires to reduce potential inhalation exposure. Efforts should be taken to clean skin and clothing as soon as possible after live-fire training to limit dermal absorption as well.