As a typical two-dimensional (2D) ferromagnetic insulator (FI), the Cr2Si2Te6 (CST) has performance ferromagnetic properties. The previous investigation has shown that quantum mechanical simulation can get structure and electronic properties of CST, and the indirect gap value of CST is 0.6 eV by establishing its layered calculation. It implies that the CST is an excellent optical modulator due to larger infrared radiation absorption interval. Based on that, some groups conducted the research of fiber laser based on CST saturable absorber (SA). However, the exploration and application of 2D CST in optics is still in the early stage. In this investigation, the CST was utilized as a SA in an Er-doped fiber laser. The dual-wavelength mode-locked pulse could be observed when the pump power was adjusted from 25 to 140 mW. The CST was applied in Er-doped fiber as SA for generating dual-wavelength mode-locked pulse for the first time. It exhibits performance optical properties that provide a significant reference for exploring the application of 2D materials in ultrafast laser.