This investigation examines the diversity and utilization of Commelinaceae in three research areas in central Laos. It identifies a total of 17 species, including Amischotolype divaricata Duist., Callisia fragrans (Lindl.) Woodson, Callisia repens (Jacq.) L., Commelina benghalensis L., Commeilina diffusa Burm.f., Cyanotis arachnoidea C.B. Clarke, Cyanotis axillaris (L.) D. Don ex Sweet, Cyanotis cristata (L.) D. Don, Floscopa scandens Lour., Murdannia edulis (Stokes) Faden, Murdannia medica (Lour.) D.Y. Hong, Murdannia nudiflora (L.) Brenan, Murdannia spectabilis (Kurz) Faden, Pollia thyrsiflora (Blume) Steud., Tradescantia pallida (Rose) D.R. Hunt, Tradescantia spathacea Sw., and Tradescantia zebrina Bosse. There are ten species within this family that are utilized for food, medicine, ornamental purposes, or as animal feed. Floscopa scandens has the highest use value (UV) of 1.24, followed closely by Commelina diffusa with a UV of 1.22. Murdannia spectabilis has the lowest UV of 0.39 and is mostly utilized for ornamental purposes. Several species, including Commelina diffusa, Floscopa scandens, and Tradescantia zebrina, are utilized in traditional medicine to address musculoskeletal ailments, provide food, and alleviate obstetric/gynecological concerns. The informant agreement ratio (IAR) is 1.00 for musculoskeletal symptoms and nourishment and 0.9 for obstetric and gynecological symptoms. The plant species Commelina diffusa, Tradescantia zebrina, and Floscopa scandens demonstrate a fidelity level (%FL) of 100% in traditional medicinal use, with Floscopa scandens showing %FL of 63.64% for back pain treatment and 36.36% for postpartum recovery.
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