Purpose This paper aims to explore the underlying intention behind using blockchain technology (BLCT) in the agri-food supply chain (AFSC). This is achieved by using a conceptual framework based on technology acceptance models that considers various factors influencing user behavior toward implementing this technology in their practices. Design/methodology/approach The conceptual framework developed is empirically validated using structural equation modeling (SEM). A total of 258 respondents from agri-food domain in India were involved in this survey, and their responses were analyzed through SEM to validate our conceptual framework. Findings The findings state that food safety and security, traceability, transparency and cost highly influence the intention to use BLCT. Decision-makers of the AFSCs are more inclined to embrace BLCT if they perceive the usefulness of the technology as valuable and believe it will enhance their productivity. Practical implications This study contributes to the existing literature by providing thorough examination of the variables that influence the intention to adopt BLCT within the AFSC. The insights aim to benefit industry decision-makers, supply chain practitioners and policymakers in their decision-making processes regarding BLCT adoption in the AFSC. Originality/value This study investigates how decision-makers’ perceptions of BLCT influence their intention to use it in AFSCs, as well as the impact of the different underlying factors deemed valuable in the adoption process of this technology.