Influence Of Seeding Density Research Articles

Influence of Seeding Density and Extracellular Matrix on Bile Acid Transport and Mrp4 Expression in Sandwich-Cultured Mouse Hepatocytes

Influence of Seeding Density and Extracellular Matrix on Bile Acid Transport and Mrp4 Expression in Sandwich-Cultured Mouse Hepatocytes

Influence of Seeding Density on Contents and Uptake of N, P and K in Linseed (Linum usitatissimum L.)

Field experiments were conducted at the University of Wales, Aberystwyth, UK to evaluate the effects of seeding density and row spacing on the contents and uptake of N, P and K in linseed during 1993 and 1994. The contents of all three nutrients decreased with age of the plants up to the start of flowering, but N and P slightly increased at maturity in both the experiments. The increase of N and P at maturity could be the result of greater accumulation of these elements in the seeds. The uptake of all three elements (N, P and K) increased progressively with age of the plants, with a peak at maturity, but the level of K decreased by almost half in both experiments between flowering and maturity. The progressive increase of uptake with age of the plants may be related to total dry matter production. The decrease of K at maturity indicated that lower plant parts (stem and leaves) accumulated a higher percentage of K than seeds. The senescence of leaves at maturity might also have contributed to the decrease of K at maturity. The characteristics of the crop were in general quite similar in 1993 and 1994. The small variations found in the contents and uptake of N, P and K could be due to variation in weather conditions and the total length of the growth period. In 1993, the weather was moist and relatively cool, while in 1994 it was dry and relatively hot.

Synthesis and Expression of Smooth Muscle Phenotype Markers in Primary Culture of Rabbit Aortic Smooth Muscle Cells: Influence of Seeding Density and Media and Relation to Cell Contractility

Rabbit aortic smooth muscle cells (SMC) were seeded at moderate or high densities and grown either in the presence of serum or in the serum-substitution formula Monomed. Expression and synthesis of marker proteins caldesmon, calponin, smooth muscle myosin, and vinculin were monitored during SMC cultivation. Contractility was tested by the ability of cultured SMC to deform silicone membranes following ionomycin treatment. The results show that cells of moderate density grown in Monomed, as opposed to those grown in 5% serum, have the smooth muscle isoform of caldesmon 1.6-fold higher, calponin 1.4-fold and smooth muscle myosin 1.4-fold higher on Day 14 of cultivation. Synthesis of these proteins corresponded to their expression in SMC. The metavinculin:vinculin ratio slightly decreased over the first days with a following reestablishment on Day 8. Contraction was observed until Day 13, compared with Day 7 for cells grown in the presence of serum. High seeding density also prevented a decrease in the expression of smooth muscle markers with the exception of smooth muscle caldesmon whose content in the high density SMC culture was not significantly different from that in the moderate density culture. The period of contractility of SMC in the high density culture was also similar to that in the moderate density culture in the presence of serum. We conclude that cultivation of primary SMC in Monomed allows the maintenance of cells in the contractile phenotype more effectively than high initial seeding density.

Influence of seeding density on multicellular organization and nuclear events in cultures of normal and neoplastic mouse mammary epithelium.

Influence of seeding density on multicellular organization and nuclear events in cultures of normal and neoplastic mouse mammary epithelium.

Influence of Seeding Density and Row Spacings on the Resistance of Spring Wheats to the Wheat Stem Sawfly1

In the spring-wheat varieties solid-stemmed Rescue and hollow-stemmed Thatcher infestation and cutting by the wheat stem sawfly (Cephus cinctus Nort.) decreased as the seeding density increased and row spacing decreased. The decrease in cutting was due in part to a lower infestation, which was in turn associated with a decrease in moisture in stems, diameter of stems, and plant height at peak of oviposition. Apparently sawflies selected the larger, more succulent stems in the lighter seedings for oviposition.