This study examines the characteristics of various scanning trajectories or patterns under the influence of scanning parameters in order to develop a theory to define their corresponding image resolutions. The lack of an accurate estimation of pixel size for a specified set of scanning parameters and their connection is a key challenge with existing scanning methods. Thus, this research aimed to propose a novel approach to estimate the pixel size of different scanning techniques. The findings showed that there is a link between pixel size and a frequency ratio NP, which is the ratio of two waveform frequencies that regulates the density of the scanning pattern. A theory has been developed in this study to explain the relationship between scanning parameters and scanning density or pixel size, which was not previously considered. This unique theory permitted the a priori estimate of the image resolution using a particular set of scanning parameters, including the scan time, frequencies, frequency ratio, and their amplitudes. This paper presents a novel and systematic approach for estimating the pixel size of various scanning trajectories, offering the user additional flexibility in adjusting the scanning time or frequency to achieve the desired resolution. Our findings also reveal that in order to achieve a high-quality image with high signal-to-noise and low error, the scanning trajectory must be able to generate a fairly uniform or regular pattern with a small pixel size.