Influence Of Preservation Solution Research Articles

Étude des caractéristiques biomécaniques sur la préservation des nerfs

Different studies have described the biomechanics of nerves. An important dispersion of outcomes can be observed and can be explained by the biological nature of tissues, the different experimental protocols like different conditions of testing and different preservation solutions that modify they biomechanical behavior. Nevertheless, the influence of preservation solution on biomechanics has not been well characterized. It is capital to quantify the amount of solution absorbed by the nerve during the preservation process before the test in order to understand the different biomechanical behaviors seen on the literature. The next experiment is focused on the characterization of the influence from preservation solution on the rat sciatic nerves biomechanics. Male 6 weeks old Wistar rat sciatic nerves were harvested ex vivo rats 300 g weight. The rats were donated after being used for microsurgery training. During the study, the nerves were preserved on 4 different preservation solutions: PBS, mineral oil, glucose 30% and NaCl0.9%. Nine rat sciatic nerves were used to test the influence of the preservation solutions chosen. The protocol used in the literature works with a unidirectional tensile test. The elastic modulus and stress strain curves were obtained with this specific software. The displacement was measured at a specific time t and the initial length lo as the initial length between the clamps. The sciatic nerve harvesting and the preservation solution have influenced different characteristics. The mineral oil and NaCl do not allow an optimal preservation of nerves. After few days of preservation, the nerve was physically damaged, deformed and even in a putrefaction state judging by the odor of the containers. The preservation modes have a remarkable influence on the physical integrity of the nerve. Additionally, an important absorption capacity of the nerve has been demonstrated. The obtained results showed a twofold increase of weight during few days (mass augmentation more than a 100%). The results have shown that PBS changes the biomechanical behavior of nerves by diminishing the elastic modulus and a by increasing the maximal strain when the solution diffuse into the material. These behavior variations are necessarily linked to the nature of nerves, because the observed variations were confirmed on the nerve microstructure. The influence of preservation solutions on biomechanical properties has been characterized on the rat sciatic nerves. PBS could be a good preservation solution given the dispersion of values during the test, a deep analysis was performed with PBS preserved nerves.

Influence of Preservation Solution on Human Islet Isolation Outcome

The influence of the preservation solution used for in situ perfusion of the donor and pancreas storage on islet isolation has received little attention. In this prospective controlled trial, we compared the outcome of human islet isolation from pancreata perfused with University of Wisconsin (UW) solution or Celsior, an alternative colloid-free extracellular solution. At the 1-year interim analysis, the viability and insulin secretion of islets isolated from donors perfused with UW (n=19) or Celsior (n=5) were identical. However, total islet recovery (IEQ) and isolation yield (IEQ/g) were 1.8-fold and 2.1-fold inferior in the Celsior group (P<0.05 vs. UW). Overall, 13 (68%) of islet preparations were effectively transplanted from the UW group vs. none from the Celsior group (P=0.01). The clinical study was discontinued and the causes of these differences were further explored in the pig (n=14). In contrast to UW, Celsior induced cell swelling and pancreas edema after only four hours of cold storage. These abnormalities were delayed when the donor was perfused with Solution de Conservation d'Organes et de Tissus (SCOT), an extracellular solution containing polyethylene glycol. Our results suggest that colloid-free preservation solutions might be suboptimal for pancreas perfusion and cold storage prior to islet isolation and transplantation. Because pancreata are now frequently recovered for islet transplantation, preliminary experimental and clinical data about islet isolation should be obtained prior to the routine implementation of new preservation solutions for abdominal perfusion during multiorgan recovery.

Influence of preservation solution on early lung function (Euro-Collins vs Perfadex)

Background This clinical study was performed to evaluate the efficiency of 2 different preservation solutions (Euro-Collins [EC] vs Perfadex [P]) on organ function in human lung transplantation. Methods The donor lungs for 46 patients were flushed either with EC solution (25 cases, EC group) or Perfadex (21 cases, P group). Transplant function was assayed using PaO2/FiO2 ratio after transplantation upon intensive care unit (ICU) arrival and at 12 and 24 hours later (T1, T2, and T3, respectively). We also compared the duration of mechanical ventilatory support and ICU stay. Results The PO2/FiO2 ratio was significantly better in the P than EC group at T1, T2, and T3. The duration of mechanical ventilatory support and ICU stay were lower also in P group, whereas age, sex, aetiology of lung disease, donor, PaO2/FiO2 ratio, and ischemia time did not show differences between the 2 groups. Conclusions Our data on graft function tend to confirm better graft preservation using the P preservation solution.

Influence of preservation solution on the morphology after cardioplegic arrest and cold storage

Influence of preservation solution on the morphology after cardioplegic arrest and cold storage

Influence of preservation solution on high energy phosphate (HEP) catabolism after single-dose cardioplegic arrest and during cold storage of donor hearts: J. Minten, S. Sukehiro, T. Möllhoff, W. Dyszkiewicz, W. Flameng, K. U. Leuven, Lab. Exp. Cardiac Surgery, Leuven, Belgium

Influence of preservation solution on high energy phosphate (HEP) catabolism after single-dose cardioplegic arrest and during cold storage of donor hearts: J. Minten, S. Sukehiro, T. Möllhoff, W. Dyszkiewicz, W. Flameng, K. U. Leuven, Lab. Exp. Cardiac Surgery, Leuven, Belgium

Retrospective Comparative Study on the Influence of Preservation Solutions on Human Renal Allograft Function and Survival

This retrospective study has revealed that human cadaveric renal grafts preserved with hypertonic citrate solution have a significantly higher proportion with primary graft function compared with EuroCollins preserved grafts. Furthermore, Hypertonic citrate-preserved grafts have better early renal function. Those grafts with primary function show an improved graft survival at 12 months when compared with those grafts which had delayed function. There was no difference in renal graft function at 6 months following transplantation between the hypertonic citrate and EuroCollins-preserved grafts nor in the graft losses from rejection. Although the overall graft failure rate in the hypertonic citrate group is higher, this is due to patient mortality and not to rejection.