Influence Of Low-intensity Laser Irradiation Research Articles

The influence of low- intensity laser irradiation versus hyperbaric oxygen therapy on transcutaneous oxygen tension in chronic diabetic foot ulcers: a controlled randomized trial.

Evaluation of the stage and severity of the chronic diabetic foot ulcer (CDFU) is vital to increase the healing rate andto select the suitable treatment. We aim to assess the influence of low-intensity laser irradiation (LILI) and hyperbaric oxygenation therapy (HBOT) to acceleratethe CDFU healing thru the transcutaneous oxygen tension (TcPO2) measurements. Seventy-five diabetic patients (type 2) of both genders, their ages ranged from 40-65years with CDFUs (duration of ulcer < 6weeks). All patients were randomly assigned into LILI, HBOT, and the control group. Measurement of TcPO2 using transcutaneous oximetry was performed for all patients once in the baseline and consequently in the second, fourth, and sixth- weeks duration. LILI utilized by a 33-diode cluster contact applicator with output power 1440 mW, energy density (fluency) was adjusted for 4J/Cm2 at 10kHz, and for 8min per session, three times per week for a total of consecutive 6weeks. HBOT was pressurized up to 2.5 ATA and patients delivered 100% oxygen for 60min per session for 30 sessions. The Control group received conventional wound care only, twice daily, with saline and apply a new bandage after cleaning. MANOVA revealed a statistically insignificant difference in the control group, while statistically significant improvement in both the LILI and HBOT groups. The intergroup comparisons showed an insignificant statistical difference in the pre-test, while highly statistically significant differences for the three post-measures in favor of HBOT and LILI groups. The percentage of improvement of the HBOT group was higher than LILI. Post-hoc test using the least significant difference (LSD) revealed statistically significant differences of HBOT in favor of the LILI group. Both LILI and HBOT may be used as adjunctive methods to improve TcPO2 that accelerate healing in CDFUs. HBOT may be favorable in the improvement of TcPO2 than LILI.

Influence of low-intensity laser radiation on the dynamics of some phytohormone content in the callus tissues of wheat Triticum aestivum L.

We carried out an analysis of the influence of low-intensity He–Ne laser radiation on the dynamics of changes observed in the content of the phytohormones abscisic, jasmonic, salicylic, and indoleacetic acids in wheat calluses of Triticum aestivum L. using chromatography–mass spectrometry. He–Ne laser irradiation (λ = 632.8 nm) in its stimulant dose (3.5 J cm−2) was proved to cause a decrease in jasmonic and indoleacetic acid content and, simultaneously, a significant (2.5–3 times) increase in ‘stress’ abscisic and salicylic acids. The response of jasmonic acid to He–Cd laser irradiation (λ = 441 nm) was specific, which is probably due to the blue-light-induced biosynthesis of the acid. This article discusses a possible connection between substantial changes in growth regulator content within the plant tissue under the influence of low-intensity laser irradiation and the stress character of this type of radiation.

The influence of low-intensity laser irradiation of blood on the lactoferrin level in the patients presenting with community-acquired pneumonia

The objective of the present study was to estimate the influence of low-intensity laser irradiation of blood on the levels of lactoferrin in the patients presenting with community-acquired pneumonia. All the patients were divided into two groups. Those comprising the control group received only medicamental therapy. The standard treatment of the patients in the second group was supplemented by intravenous laser irradiation of blood (IVLIB-405 technique). Each irradiation session performed in a continuous mode lasted 5-7 minutes, with the total treatment course consisting of 7 daily procedures. Lactoferrin was determined before and after the treatment by the enzyme immunoassay with the use of "Lactoferrin Strip" kits ("Vektor-Best- Yug" JSC, Russia). Analysis of the results of the study revealed a significant decrease in the blood lactoferrin level down to the normal range in the patients treated with the use of laser therapy. The application of intravenous laser irradiation of blood for the combined treatment of the patients with community-acquired pneumonia was accompanied by normalization of the blood lactoferrin level and the improvement of the clinical course of the disease.

Influence low-intensity laser irradiation on the ultrastructural organization of loach embryo cells

The ultrastructural organization of loach embryo cells (Misgurnus fossilis L.) at the stage of the first and the tenth embryo divisions was investigated under the influence of low intensity helium-neon laser irradiation of 5 min exposure. It was determined that the effect of laser irradiation led to ultrastructural changes: the shape of mitochondria and multivesicular bodies changes. The obtained results explain the possible mechanism of influence of low-intensity laser irradiation at the cellular level.