Influence Of Host Plant Research Articles

Increased microbial complexity and stability in rhizosphere soil: A key factor for plant resilience during mining disturbance

The network-based complexity and stability of the microbial community are critical for host fitness under disturbance, but there are still gaps in our understanding of whether there are general rules governing this relationship. Despite evidence that the rhizosphere microbiome plays an important role in host fitness, it is unclear whether rhizosphere microbial complexity and stability influence host plant fitness under scenarios of environmental disturbance. Here, we investigated the effects of mining disturbance on the complexity and stability of the rhizosphere microbiome and its potential role in plant fitness. Our findings demonstrated that, compared with mildly disturbed mining sites, severely disturbed mining sites exhibited significantly increased complexity and stability indices of the rhizosphere microbial community. Furthermore, we identified a positive feedback relationship between microbial complexity and stability and the functional potential of the microbial community, which ultimately benefits plant fitness. Our study provides empirical evidence that mining disturbance increases microbial complexity and stability, thereby increasing the resilience of host plants to environmental disturbance. Understanding microbially mediated tolerance to mining disturbance may improve our ability to predict and manage plant adaptability in changing environments.

Influence of Temperature and Host Plant on the Digestion of Frankliniella intonsa (Trybom) Revealed by Molecular Detection.

Frankliniella intonsa (Trybom) (Thysanoptera: Thripidae) is an important type of thrip and a polyphagous pest, which poses a serious threat to many crops, especially those in tropical regions of China. Its feeding behavior and the damage caused vary among different host plant species and are affected by ambient temperature and plant nutrients as well. The digestion rate is an important index for directly observing the digestion process, but there have been no studies directly measuring the digestion in thrips under the influence of different temperatures and host plants. Here, the digestion rate of F. intonsa was assessed by using a molecular diagnostic tool. We also determined the nutrient content in three host plant (mango, cowpea, and pepper), including soluble proteins, free fatty acids, soluble sugars, and water. The results showed that the high and low temperatures (16 °C and 32 °C) both seemed to accelerate the digestion of F. intonsa compared to the optimal temperature (26 °C) and the protein content of plants played an important role in the digestive response of F. intonsa to temperature changes. The findings can help reveal the feeding damage caused by F. intonsa to different plants and help to better understand its feeding ecology, according to its interaction with the host plant.

Nutrient requirements shape the preferential habitat of Allorhizobium vitis VAR03-1, a commensal bacterium, in the rhizosphere of Arabidopsis thaliana.

A diverse range of commensal bacteria inhabit the rhizosphere, influencing host plant growth and responses to biotic and abiotic stresses. While root-released nutrients can define soil microbial habitats, the bacterial factors involved in plant-microbe interactions are not well characterized. In this study, we investigated the colonization patterns of two plant disease biocontrol agents, Allorhizobium vitis VAR03-1 and Pseudomonas protegens Cab57, in the rhizosphere of Arabidopsis thaliana using Murashige and Skoog (MS) agar medium. VAR03-1 formed colonies even at a distance from the roots, preferentially in the upper part, while Cab57 colonized only the root surface. The addition of sucrose to the agar medium resulted in excessive proliferation of VAR03-1, similar to its pattern without sucrose, whereas Cab57 formed colonies only near the root surface. Overgrowth of both bacterial strains upon nutrient supplementation inhibited host growth, independent of plant immune responses. This inhibition was reduced in the VAR03-1 ΔrecA mutant, which exhibited increased biofilm formation, suggesting that some activities associated with the free-living lifestyle rather than the sessile lifestyle may be detrimental to host growth. VAR03-1 grew in liquid MS medium with sucrose alone, while Cab57 required both sucrose and organic acids. Supplementation of sugars and organic acids allowed both bacterial strains to grow near and away from Arabidopsis roots in MS agar. These results suggest that nutrient requirements for bacterial growth may determine their growth habitats in the rhizosphere, with nutrients released in root exudates potentially acting as a limiting factor in harnessing microbiota.

Influence of host plant on the wing shape of Bactrocera cucurbitae and Bactrocera tau (Diptera: Tephritidae)

ABSTRACT Bactrocera fruit flies are a significant pest of fruits and vegetables. Some of them are polyphagous and show rapid reproduction. Our study evaluated the impact of three host plants: bottle gourd (Lagenaria siceraria), ridge gourd (Luffa acutangula), and cucumber (Cucumis sativus) on the shape and size of the wings of Bactrocera cucurbitae and B. tau by using geometric morphometrics. The wings of females were significantly larger than those of males, and the wings of B. cucurbitae were significantly smaller than those of B. tau. Individuals feeding on different host plants did not differ significantly in wing size. Wing shape differed significantly both between sexes and between species. Differences in wing shape between males and females were larger than differences between B. cucurbitae and B. tau. Wing shape can be used to identify males and females of the two species without errors. Wing shape differed significantly between males of the two species feeding on cucumber and bottle gourd. Wing shape can be used to identify males of B. cucurbitae and B. tau feeding on those two host plants without errors.

Impact of different host plants on biology and morphometrics of various developmental stages of Papilio demoleus (Linn.)

The comparative studies under laboratory conditions have been carried out to investigate the influence of different alternative host plants viz., bael [Aegle marmelos (L.)], curry leaves [Murraya koenigii (L.) Spreng.], ber (Zizyphus mauritiana Lam.) and lemon [Citrus limon (L.) Burm.f.] on biology and morphometrics of Lemon butterfly, Papilio demoleus (Linn.). The lemon is the most preferred host of P. demoleus used as a controlled experimental host plant. The variations in periods of larval, pupal and rate of emergence into adult have been recorded and found that the lemon was the most preferred host plant on which all the larval stages survived smoothly. The caterpillars when reared on alternative hosts showed an increase in average larval period. The average survival percent of larvae were reported highest on bael leaves, followed by curry leaves and ber leaves. Similar patterns of results were also recorded on the pupal period and rate of emergence with alternative hosts. The morphometric variations in weight, size and colour of caterpillars, pupae and adults were also measured and found in decreasing order of host preferences as lemon[greater than]bael[greater than]curry[greater than]ber.

Foraging ants affect community composition and diversity of phyllosphere fungi on a myrmecophilous plants, Mallotus japonicus.

Many microorganisms inhabit the aboveground parts of plants (i.e. the phyllosphere), which mainly comprise leaves. Understanding the structure of phyllosphere microbial communities and their drivers is important because they influence host plant fitness and ecosystem functions. Despite the high prevalence of ant-plant associations, few studies have used quantitative community data to investigate the effects of ants on phyllosphere microbial communities. In the present study, we investigated the effects of ants on the phyllosphere fungal communities of Mallotus japonicus using high-throughput sequencing. Mallotus japonicus is a myrmecophilous plants that bears extrafloral nectaries, attracting several ant species, but does not provide specific ant species with nest sites like myrmecophytes do. We experimentally excluded ants with sticky resins from the target plants and collected leaf discs to extract fungal DNA. The ribosomal DNA internal transcribed spacer 1 (ITS1) regions of the phyllosphere fungi were amplified and sequenced to obtain fungal community data. Our results showed that the exclusion of ants changed the phyllosphere fungal community composition; however, the effect of ants on OTU richness was not clear. These results indicate that ants can change the community of phyllosphere fungi, even if the plant is not a myrmecophyte.

The Hidden World of Insect-Plant Interactions: a review

This review examines the hidden world of plant-insect interactions by emphasizing on the sensory perception and behavior of phytophagous insects, nutritional influence on insect reproduction, host plant resistance, Insects, weed and crop interaction, Insect pollinator plant interaction, tri-trophic interaction, and insect biotechnology. It explores how insects use sensory cues to forage for food, find mates, perceive dangers, and navigate their environment. It also examines the influence of host plants on insect behavior and the use of chemical cues for communication. The potential use of semio-chemicals in pest management for sustainable agriculture is highlighted. Nutritional factors and their impact on insect reproductive success are also discussed, emphasizing the need for balanced diets. The different categories of host plant resistance and their effects on insects are examined. The interdependent relationships between insects, plants, and weeds in agricultural ecosystems are explored, with a focus on the role of insect pollinators. The decline of insect pollinators and the importance of studying them are emphasized. The importance of tri-trophic interactions in maintaining ecological balance and biodiversity is discussed. Moreover, the role of biotechnological techniques like genomics, proteomics, transcriptomics, and epigenetics in understanding insect plant interactions as well as developing insect pest control strategies is discussed. The potential use of natural products produced by plants in environmentally friendly pest control methods is also examined. Overall, this review provides a comprehensive exploration of insect-plant interactions and the potential for sustainable pest control methods.

Host plants directly determine the α diversity of rhizosphere arbuscular mycorrhizal fungal communities in the National Tropical Fruit Tree Field Genebank

Arbuscular mycorrhizal fungi (AMF) typically exhibit reduced diversity in nutrient-rich soils. However, whether the influence of host plants on AMF within the rhizosphere is direct or indirect through the alteration of soil nutrient levels has yet to be determined. This study explored the diversity and colonization of AMF in the National Tropical Fruit Tree Genebank, where chemical fertilizers are used to preserve minor tropical fruit germplasms. We aimed to understand the direct and indirect effects of host plants on AMF community dynamics, sporulation, and colonization. By analyzing fine roots and rhizosphere soil from 12 tropical fruit trees, we assessed mycorrhizal colonization indices, soil nutrients, AMF spore density, and community structure. Despite their low colonization density and arbuscular abundance, all the tree roots harbored AMF, with spore densities ranging from 24.00 to 204.80 spores/100 g of dry soil. High-throughput sequencing identified 207 AMF virtual taxa (VTs) from 60 soil samples, with dominant taxa, including early-evolved Paraglomus fungi and ruderal AMF, being minimally affected by soil nutrient levels. Interestingly, there was no correlation between α diversity and spore density. Structural equation modeling (SEM) indicated that host plant evolutionary divergence time (ET) directly influenced AMF α diversity indices and indirectly impacted spore density via soil nutrients. However, neither ETs nor soil nutrients significantly affected the AMF community structure, with only arbuscular abundance showing a negative correlation with ET. This research underscores the intricate relationship between host plants and AMF in genebanks, offering insights for effective AMF resource management and informed conservation practices.Graphical

Halyomorpha halys (Hemiptera: Pentatomidae) trap captures at orchard and nonorchard sites and the influence of uncultivated woody host plants in adjoining woodlots.

The invasive Halyomorpha halys (Hemiptera: Pentatomidae) has threatened Mid-Atlantic tree fruit since 2010. To identify factors underlying observed differences in H. halys pest pressure among individual orchards within a geographically proximate area, a 3-yr study was conducted across 10 apple orchard and 8 nonorchard sites bordered by unmanaged woodlots. At each site, 3 pheromone traps were monitored weekly for H. halys captures from late April to mid-October. Apple injury was assessed at harvest at orchard sites annually, and a survey of woody plants found in woodlots adjacent to all sites was conducted. There were no significant differences in captures between orchard and nonorchard site types, but captures were significantly different among individual orchard sites and among individual nonorchard sites. A significant positive relationship between the amount of stink bug injury on apple at harvest and late season captures was detected at orchard sites in 2018 and 2019. Among woodlots adjacent to all sites, a significant positive relationship between the proportion of Lonicera spp. and mid- and late-season nymphal captures was identified. Season-long nymphal captures were positively related to the proportion of Lonicera and Elaeagnus and negatively with Sassafras. For adults, captures were negatively related to the proportion of Ailanthus and positively related to the proportion of Fraxinus in the early and mid-season, respectively. Our results indicate that orchard presence was not driving the relative abundance of localized H. halys populations and that differences in relative densities among sites point to other factors, such as abundance of specific uncultivated woody hosts in unmanaged areas.

Artificially applied late-terminal drought stress in the field differentially affects Ramularia leaf spot disease in winter barley

Ramularia leaf spot disease (RLS) is one of the most dominating fungal diseases in barley. The disease typically appears late in the season after flowering and results in a rapid loss of photosynthetic leaf area. A recent decline in fungicide efficacy and a lack of RLS-resistant cultivars hamper effective control. Global warming will provoke increasing droughts which influence host plant physiology and probably affect outbreak and severity of RLS. Relatively little is known about genetic resistance to RLS in winter barley and about the influence of various weather conditions and climate change on RLS pathogenesis. Hence, we evaluated severity of RLS on 15 winter barley genotypes under persistent late-terminal drought stress or controlled irrigation, respectively, in a field rainout shelter. Over three consecutive years, we observed reproducible differences in quantitative RLS field resistance of the used cultivars and variable suppression of RLS under drought. Our results support a function of drought in suppression of RLS in winter barley, but also reveal strong year effects even under semi-controlled rainout shelter conditions. Data may be relevant for genotype selection in breeding programmes for RLS resistance and for farmers in the frame of integrated disease management under a changing climate.Graphical abstract

Nutrient supply and accessibility in plants: effect of protein and carbohydrates on Australian plague locust (Chortoicetes terminifera) preference and performance.

In contrast to predictions from nitrogen limitation theory, recent studies have shown that herbivorous migratory insects tend to be carbohydrate (not protein) limited, likely due to increased energy demands, leading them to preferentially feed on high carbohydrate plants. However, additional factors such as mechanical and chemical defenses can also influence host plant choice and nutrient accessibility. In this study, we investigated the effects of plant protein and carbohydrate availability on plant selection and performance for a migratory generalist herbivore, the Australian plague locust, Chortoicetes terminifera. We manipulated the protein and carbohydrate content of seedling wheat (Triticum aestivum L.) by increasing the protein:carbohydrate ratio using nitrogen (N) fertilizer, and manipulated the physical structure of the plants by grinding and breaking down cell walls after drying the plants. Using a full factorial design, we ran both choice and no-choice experiments to measure preference and performance. We confirmed locust preference for plants with a lower protein-carbohydrate ratio (unfertilized plants). Unlike previous studies with mature wild grass species, we found that intact plants supported better performance than dried and ground plants, suggesting that cell wall removal may only improve performance for tougher or more carbohydrate-rich plants. These results add to the growing body of evidence suggesting that several migratory herbivorous species perform better on plants with a lower protein:carbohydrate ratio.

Drivers of bacterial and fungal root endophyte communities: understanding the relative influence of host plant, environment, and space

Bacterial and fungal root endophytes can impact the fitness of their host plants, but the relative importance of drivers for root endophyte communities is not well known. Host plant species, the composition and density of the surrounding plants, space, and abiotic drivers could significantly affect bacterial and fungal root endophyte communities. We investigated their influence in endophyte communities of alpine plants across a harsh high mountain landscape using high-throughput sequencing. There was less compositional overlap between fungal than bacterial root endophyte communities, with four 'cosmopolitan' bacterial OTUs found in every root sampled, but no fungal OTUs found across all samples. We found that host plant species, which included nine species from three families, explained the greatest variation in root endophyte composition for both bacterial and fungal communities. We detected similar levels of variation explained by plant neighborhood, space, and abiotic drivers on both communities, but the plant neighborhood explained less variation in fungal endophytes than expected. Overall, these findings suggest a more cosmopolitan distribution of bacterial OTUs compared to fungal OTUs, a structuring role of the plant host species for both communities, and largely similar effects of the plant neighborhood, abiotic drivers, and space on both communities.

Host plant-mediated effects on Buchnera symbiont: implications for biological characteristics and nutritional metabolism of pea aphids (Acyrthosiphon pisum).

The pea aphid, Acyrthosiphon pisum, is a typical sap-feeding insect and an important worldwide pest. There is a primary symbiont-Buchnera aphidicola, which can synthesize and provide some essential nutrients for its host. At the same time, the hosts also can actively adjust the density of bacterial symbiosis to cope with the changes in environmental and physiological factors. However, it is still unclear how symbionts mediate the interaction between herbivorous insects' nutrient metabolism and host plants. The current study has studied the effects of different host plants on the biological characteristics, Buchnera titer, and nutritional metabolism of pea aphids. This study investigated the influence of different host plants on biological characteristics, Buchnera titer, and nutritional metabolism of pea aphids. The titer of Buchnera was significantly higher on T. Pretense and M. officinalis, and the relative expression levels were 1.966±0.104 and 1.621±0.167, respectively. The content of soluble sugar (53.46±1.97µg/mg), glycogen (1.12±0.07µg/mg) and total energy (1341.51±39.37µg/mg) of the pea aphid on V. faba were significantly higher and showed high fecundity (143.86±11.31) and weight (10.46±0.77µg/mg). The content of total lipids was higher on P. sativum and T. pretense, which were 2.82±0.03µg/mg and 2.92±0.07µg/mg, respectively. Correlation analysis found that the difference in Buchnera titer was positively correlated with the protein content in M. officinalis and the content of total energy in T. pratense (P < 0.05). This study confirmed that host plants not only affected the biological characteristics and nutritional metabolism of pea aphids but also regulated the symbiotic density, thus interfering with the nutritional function of Buchnera. The results can provide a theoretical basis for further studies on the influence of different host plants on the development of pea aphids and other insects.

Mycorrhizae influence plant vegetative and floral traits and intraspecific trait variation.

Arbuscular mycorrhizal fungi (AMF) can strongly influence host plant vegetative growth, but less is known about AMF effects on other plant traits, the relative impacts of AMF on vegetative growth versus floral traits, or AMF-induced intraspecific variation in traits. In an experimental greenhouse study, we inoculated seven species of wildflowers with six species of AMF in a factorial design. We assessed how the AMF-forb combinations influenced plant survival, vegetative biomass, and floral traits and whether AMF effects on floral traits were similar in magnitude and direction to effects on vegetative biomass. For one forb species, we investigated intraspecific plant trait variation within and across AMF treatments. AMF species varied from negative to positive in their effects on host plants. AMF often had inconsistent effects on vegetative biomass versus floral traits, and therefore, quantifying one or the other may provide a misleading representation of potential AMF effects. AMF treatments generated key variation in plant traits, especially floral traits, with potential consequences for plant-pollinator interactions. Given increased intraspecific trait variation in Linum lewisii plants across AMF species compared to uninoculated individuals or single AMF treatments, local AMF diversity and their host plant associations may scale up to influence community-wide patterns of trait variation and species interactions. These results have implications for predicting how aboveground communities are affected by belowground communities. Including AMF effects on not just host plant biomass but also functional traits and trait variation will deepen our understanding of community structure and function, including pollination.

Sorghum cuticular waxes influence host plant selection by aphids.

Quantification of cuticular waxes coupled with insect bioassays and feeding behavior analysis demonstrate that long-chain C32 fatty alcohol impacts host plant selection by aphids. Cuticular waxes constitute the first point of contact between plants and their environment, and it also protect plants from external stresses. However, the role of waxes in Sorghum bicolor (sorghum) against sugarcane aphid (Melanaphis sacchari), a relatively new and devastating pest of sorghum in the U.S., is not fully understood. In this study, we monitored sugarcane aphid behavior on two genotypes of young sorghum plants with different wax chemistry: a wild-type plant (bloom) with lower C32 alcohol cuticular wax, and a mutant plant (bloomless) with 1.6 times the amount of wax compared to wild-type plants. No-choice aphid bioassays revealed that sugarcane aphid reproduction did not vary between wild-type and the bloomless plants. Electrical Penetration Graph (EPG) monitoring indicated that the sugarcane aphids spent comparable amount of time feeding from the sieve elements of the wild-type and bloomless plants. However, aphids spent more time feeding on the xylem sap of the bloomless plants compared to the wild-type plants. Furthermore, aphid choice assays revealed that the sugarcane aphids preferred to settle on bloomless compared to wild-type plants. Overall, our results suggest that cuticular waxes on young sorghum leaves play a critical role in influencing host plant selection by sugarcane aphids.

Biotic Potential Induced by Different Host Plants in the Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae).

Simple SummaryFall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is an economically important insect pest of corn crops globally. However, some other host plants on which this pest can successfully complete its generation have also been reported. Our main objective was to study the biology of fall armyworm feeding on maize, sorghum, wheat, and rice. Our overall findings show that maize is the most preferred host plant; however, the pest completed the life cycle successfully on sorghum and wheat. The survival rate was low when rice leaves were provided to larvae as diet, suggesting that rice is a non-preferred host plant.Fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a polyphagous insect pest of many important crops. To evaluate the influence of host plants on the biology and survival of the Pakistani population of S. frugiperda, we examined life table parameters of S. frugiperda raised on maize, sorghum, wheat, and rice. The development rate was significantly higher on the maize crop than on the other three host plants. Different larval diets affected development time and fecundity. S. frugiperda attained the fastest larval development (16 days) on maize and the slowest development (32.74 days) on rice. Adult females from maize-fed larvae laid 1088 eggs/female, those from sorghum-fed larvae laid 591.6 eggs/female, those from wheat-fed larvae laid 435.6 eggs/female, and those from rice-fed larvae laid 49.6 eggs/female. Age stage-specific parameters also indicated the higher fecundity, higher life expectancy, and higher survival of S. frugiperda on maize plants than on the other three hosts. Larval diets had a significant varying effect on the finite and intrinsic increase rates, reflecting that maize was the most suitable diet. The findings of the present study are useful for predicting population dynamics especially in areas cultivating Poaceae crops, except maize, to develop sustainable integrated pest management strategies for this pest.

Influence of host plants on the efficacy of tomato fruitworm parasitoids

Influence of host plants on the efficacy of tomato fruitworm parasitoids

Identifying the natural reserve area of Cistanche salsa under the effects of multiple host plants and climate change conditions using a maximum entropy model in Xinjiang, China.

Cistanche salsa (C. A. Mey.) G. Beck, a holoparasitic desert medicine plant with multiple hosts, is regarded as a potential future desert economic plant. However, as a result of excessive exploitation and poaching, its wild resources have become scarce. Thus, before developing its desert economic value, this plant has to be protected, and the identification of its natural reserve is currently the top priority. However, in previous nature reserve prediction studies, the influence of host plants has been overlooked, particularly in holoparasitic plants with multiple hosts. In this study, we sought to identify the conservation areas of wild C. salsa by considering multiple host–plant interactions and climate change conditions using the MaxEnt model. Additionally, a Principal Component Analysis (PCA) was used to reduce the autocorrelation between environmental variables. The effects of the natural distribution of the host plants in terms of natural distribution from the perspective of niche similarities and extrapolation detection were considered by filtering the most influential hosts: Krascheninnikovia ceratoides (Linnaeus), Gueldenstaedt, and Nitraria sibirica Pall. Additionally, the change trends in these hosts based on climate change conditions combined with the change trends in C. salsa were used to identify a core protection area of 126483.5 km2. In this article, we corrected and tried to avoid some of the common mistakes found in species distribution models based on the findings of previous research and fully considered the effects of host plants for multiple-host holoparasitic plants to provide a new perspective on the prediction of holoparasitic plants and to provide scientific zoning for biodiversity conservation in desert ecosystems. This research will hopefully serve as a significant reference for decision-makers.

Arbuscular mycorrhizal colonization increases plant above-belowground feedback in a northwest Chinese coal mining-degraded soil by increasing photosynthetic carbon assimilation and allocation to maize.

A three-compartment culture system was used to study the mechanism by which the AM fungus Funneliformis mosseae influences host plant growth and soil organic carbon (SOC) content in a northwest China coal mining area. A 13CO2 pulse tracing technique was used to trace the allocation of maize photosynthetic C in shoots, roots, AM fungus, and soil. Carbon accumulation and allocation in mycorrhizal (inoculated with Funneliformis mosseae) and non-mycorrhizal treatments were detected. AM fungal inoculation significantly increased the 13C concentration and content in both above- and below-ground plant parts and also significantly enhanced anti-aging ability by increasing soluble sugars and catalase activity (CAT) in maize leaves while reducing foliar malondialdehyde content (MDA) and leaf temperature and promoted plant growth. AM fungi also increased P uptake to promote maize growth. Soil organic carbon (SOC), glomalin, microbial biomass carbon (MBC), and nitrogen (MBN) contents increased significantly after inoculation. A mutually beneficial system was established involving maize, the AM fungus and the microbiome, and the AM fungus became an important regulator of C flux between the above- and below-ground parts of the system. Inoculation with the AM fungus promoted plant growth, C fixation and allocation belowground to enhance soil quality. A positive above-belowground feedback appeared to be established.

First Report of Fungal Endophyte Communities and Non-Defensive Phytochemistry of Biocontrol-Inoculated Whitebark Pine Seedlings in a Restoration Planting

Plant endosymbionts (endophytes) influence host plant health and express genotype-dependent ecological relationships with plant hosts. A fungal species intended to confer host plant resistance to a forest pathogen was used as inoculum to test for effects of inoculation on disease resistance, microbiomes, and phytochemistry of a threatened pine species planted in a restoration setting. Correlations of inoculation presence/absence, phytochemistry, spatial location of seedlings, maternal seed sources, and fungal endophytic communities in the foliage of six-year-old whitebark pine (Pinus albicaulis) seedlings were assessed five years after an experimental inoculation of seedlings with foliar endophytic fungi cultured from whitebark pine trees at Crater Lake National Park, including Paramyrothecium roridum. We hypothesized that P. roridum would modify host microbiomes in a manner that combats white pine blister rust disease. Our assessment of seedlings in the field five years after inoculation allowed us to consider whether inoculation stimulated long-lasting changes in microbiome communities and whether effects varied by seedling genetic family. Tests for effects of endophyte inoculation on disease resistance were inconclusive due to current low levels of rust infection observed at the field site. Foliar fungal endophyte richness and Shannon diversity varied with maternal seed sources. Isotopic stoichiometry and phytochemistry did not vary with seedling spatial proximity, inoculation treatment, or maternal seed family. However, endophyte community composition varied with both seedling spatial proximity and maternal seed sources. Endophytic communities did not vary with the inoculation treatment, and the hypothesized biocontrol was not detected in inoculated seedlings. We draw three conclusions from this work: (1) fungal microbiomes of whitebark pine seedlings across our study site did not vary with host phytochemical signatures of ecophysiological status, (2) the inoculation of P. albicaulis seedlings with a mixture of fungal endophytes did not lead to persistent systemic changes in seedling foliar microbiomes, and (3) in correspondence with other studies, our data suggest that maternal seed source and spatial patterns influence fungal endophyte community composition.