Influence Of Effective Microorganisms Research Articles

The influence of effective microorganisms on the yield and quality of individual seed components of different soybean genotypes

The influence of effective microorganisms on the yield and quality of individual seed components of different soybean genotypes

Influence of Effective Microorganisms and Clinoptilolite on Gut Barrier Function, Intestinal Health and Performance of Broiler Chickens during Induced Eimeria tenella Infection

The prohibition of certain coccidiostats in poultry has created a need to seek an alternative to control Eimeria infection. The aim of this study was to evaluate the effects of effective microorganisms (EM) in a multi-strain probiotic (Bokashi®), with clinoptilolite as a feed supplement on the mRNA expression of tight junction proteins and redox enzymes in the caecal tissue of chickens infected with E. tenella. The integrity of the intestinal barrier was tested by determining the concentration of fluorescein isothiocyanate dextran (FITC-d) in the chicken’s serum. A total of 600 1-day-old Ross 308 male chickens received diets with a 0.5% or 0.8% concentration of the probiotic together with clinoptilolite. The experiment used 5 treatment groups, and a control group, each with 5 replicates with 20 birds. The results indicate that the use of the 8 kg/t of feed multi-strain probiotic together with clinoptilolite in the diet of poultry caused a significant reduction in the number of E. tenella oocysts in the faeces and caecum and significantly improved the growth rate of chicken broilers infected with E. tenella. In addition, the probiotic and clinoptilolite enhanced antioxidant processes in the caecal mucosa and reduced oxidative stress induced by E. tenella infection.

Influence of Effective Microorganisms, Colloidal Nanosilver and Silver Compounds on Water Content in New and Used Engine Oil: A Preliminary Study

This paper presents research upon the impact of ecological measures on the water content of new and used oil. Water and microorganisms are major factors of contamination for engine oils and fuels, and they lead to a significant reduction in the lifetime and performance of engines. The microorganisms occur naturally in the environment, from which they enter into the oil and fuel. Despite various preventive measures, it is not possible to completely remove water from petrochemical products. That is why the protection against and prevention of the various types of contamination of petroleum products, especially microbiological contamination, is very important. Biocides are one example of the agents used for the prevention of contamination; biocides belong to a group of pesticides that are used to eliminate microbial contamination. Due to the fact that currently available methods are ineffective and often have a detrimental effect on the natural environment, research is underway to discover modern and ecological measures to combat the phenomenon of the microbiological contamination of petroleum products. This paper shows the effects of environmentally friendly additives on the water content in lubricating oil, i.e., whether these additives cause the release of water bound in the oil, and whether their composition increases the water content of the oil. Samples of new and used oil were utilized for the tests. Effective microorganisms (EM), in both liquid form and within ceramic tubes, were added to the new and used oil samples. In addition, silver solution and silver compounds were added in the same amounts as the liquid effective microorganisms. In order to confirm and compare the obtained test results, the dynamic viscosity, flash point, acid number, base number, and water content were measured, and these are presented in this study. These measurements were followed by the analysis of the influence of the additives on the water content. It was found that the adding of effective microorganisms to fresh oil in liquid form caused a significant increase in the water content of the oil; in the case of a smaller amount (2.5 mL), the water content more than doubled, and in the case of a larger amount, the water content exceeded the measuring range of the device. Next, an evaluation of the impact of these environmental measures on the water content was carried out. It was found that the adding of liquid effective microorganisms to new oil caused a significant increase in the water content of the oil; the water content more than doubled for the addition of 2.5 mL, while for a larger addition (5 mL), the water content exceeded the measuring range of the device. The same was true for the addition of silver compounds, regardless of their type and amount. The best results were obtained with ceramic effective microorganisms, but the results for silver cannot be presented due to the over-titration of the device (the amount of water exceeded the measuring range). For used oil, the liquid effective microorganism was found to give the best result.

Influence of effective microorganisms on pikeperch nonspecific humoral immunity, general condition, and development

Abstract Products containing effective microorganisms (EMTM) use microorganisms to work in the environment in which they are applied. EMTM is used in many countries worldwide, mainly in agriculture, including in aquaculture, and in environmental protection. Fish farmers use these products to stimulate effective growth and conditioning and as immunity enhancers. The aim of the study was to evaluate the effect of EMTM Probiotyk (Greenland, Poland) on the development of humoral non-specific resistance in pikeperch (Sander lucioperca) in the initial stage of rearing in recirculating aquaculture systems (RAS). The experimental diet was administered for 28 days with 0% (control group), 2%, and 4% EMTM supplementation. The results from the humoral parameters (Lys, TP, Ig) showed no statistical differences. The only statistically significant difference was noted in Cer for the 4% group. Non-specific humoral-mediated immunity in fish plays a key role in defense against damaging factors. Pikeperch is a fish used for restocking open waters, where fish can be subjected to agrotechnical treatments, including EMTM. The results permitted estimating the potential risks of using EMTM in aquaculture.

The Effect of Additional Effective Microorganisms in Vegetative Growth of Aerobic Paddy

The influence of effective microorganisms (EM), microbial inoculant containing yeasts, fungi, bacteria and actinomycetes was evaluated in field trials of Aerob 1 paddy cultivation in Jasin, Melaka. Aerobic paddy is a new way of rice cultivation in areas where water resources have been scare and affected by the climate change. As water shortage and climate change is becoming severe, the technology of growing rice with aerobic rice systems need to be further refined or developed to ensure the quality of rice production in water-short areas. The objective of this study was to determine the effect of additional EM on the growth pattern, and to evaluate the efficiency of EM uptake on the aerobic plant. This experiment was carried out by using random controlled Randomized Controlled Block Design (RCBD) consisted of three treatments with two replications in four blocks. Aerobic seed cv. Aeron1 was used as planting materials and has been applied by three different treatments of EM during vegetative growth. The treatment for this study was T1 = 1.8 g NPK with urea (control) while T2 was 1.8 g NPK and T3, with 0.9 g NP with 100 mL of EM respectively during 15 (early vegetative stage) and 45 DAS (late vegetative stage). The RCBD experimental design was used with two replications in each treatment in four blocks. There are three series of harvesting (35, 50 and 60 DAS) was conducted throughout this study. The growth parameter studied was shoot and root dry biomass, number of tillers, RGR at each of harvesting. Our finding revealed that the application of EM did not improve plant growth parameter but the growth pattern of T2 and T3 showed steady improvement, although not significant compared to T1. The application of EM at different growth stages did not enhance the relative plant growth rate of Aeron 1 under aerobic condition, but in comparison to T1 and T2, T3 treatment with 50% reduction of NPK and EM could significantly reduce the cost of land management and fertilizer.

Influence of Effective Micro-Organisms (EM) and some Antioxidant on Storability and Fruit Quality of Mandarin under Cold Storage Conditions

Influence of Effective Micro-Organisms (EM) and some Antioxidant on Storability and Fruit Quality of Mandarin under Cold Storage Conditions

Water quality and phytoplankton structure changes under the influence of effective microorganisms (EM) and barley straw – Lake restoration case study

Many lakes worldwide, especially shallow, experience great changes due to eutrophication, manifested in severe, usually toxic water blooms, disqualifying them from recreation. In order to improve water quality, restoration programs are implemented, including numerous methods. Intense nutrient cycling resulting from detrimental role of sediments impede obtaining of clear water state. One of the restoration methods proposed in recent years was Effective Microorganisms (EM), i.e. the set of microorganisms aiming at the inhibition of harmful bacteria through competitive exclusion. This approach was introduced in shallow Konin Lake (Western Poland), suffering from severe cyanobacterial water blooms. Prior to the treatment, protective action was conducted i.e. the elimination of external nutrient loads with backwater from the river. Changes in water chemistry, phytoplankton structure and macrophytes distribution were noted during the 5-year studies (2011–2015), covering the treatment (2013–2015) as well as two previous years. Oscillatoriacean cyanobacteria were most abundant in (2011−2012), while Nostocales in summer 2014–2015, as a result of decreased phosphorus but increased nitrogen concentrations. Slight increase in Cladoceran zooplankton was observed, but none in submerged macrophytes due to low water transparency. EM application initiated positive changes in the ecosystem by means of excessive organic matter decomposition and increased diversity of phytoplankton, nevertheless cyanobacteria blooms were still present due to high nutrient content.

Influence of effective microorganisms on the dry matter yield and chemical composition of meadow vegetation

Influence of effective microorganisms on the dry matter yield and chemical composition of meadow vegetation

Influence of effective microorganisms on the non-specific cellular defence mechanisms of pikeperch

Effective Microorganisms (EM) technology uses microorganisms to work in the surrounding environment. Complex microbiological preparations show synergistic effects, combining the effects of probiotics, prebiotics and synbiotics. EMTM technology is well-known and used in 120 countries worldwide, mainly in agriculture, including aquaculture, and in environmental protection. Fish farmers use these preparations to enhance fish growth, condition and immunity. The aim of the study was to evaluate the effect of EM-Probiotic (Greenland) on the development of cellular non-specific resistance in pikeperch (Sander lucioperca) at the initial stage of rearing in a recirculating aquaculture system (RAS). The experimental diet was administered for 28 days with EM supplementation at 0% (control group), 2% and 4% of the feed. Water temperature in the RAS was maintained at 22.0ºC. Hematopoietic organs (spleen and kidney) were collected twice, after 28 days of EM supplementation (first term) and 28 days after cessation of supplementation (second term). The respiratory burst activity (RBA) and potential killing activity (PKA) of macrophages, as well as the proliferative activity of T- and B-lymphocytes (MTT assay) were assessed. The results of the study show that EM at the initial stage of pikeperch development (body weight of 2 to 14 g) suppress cellular defence mechanisms, decreasing the activity of immunocompetent cells in RBA, PKA and MTT tests. The results from the first organ harvesting showed differentiated EM activity at a statistically insignificant level (P>0.05). In samples collected 28 days later, the inhibitory effect was demonstrated at a statistically significant level (P<0.05) in all parameters in both experimental groups. Non-specific cellular immunity in fish plays a key role in defence against damaging factors, including pathogenic ones. Since fish in an RAS are also susceptible to potentially pathogenic agents, immunosuppression of these mechanisms may aggravate the disease..

Influence of Effective Microorganisms on Growth Performances of Broiler Chicken under Local Conditions

The study was conducted to assess the effects of Effective Microorganisms (EM) as an additive to drinking water on growth performance of broiler chicken under local conditions. Hundred and ninety eight, day-old chicks were randomly allocated into two treatments, in a Complete Randomized Design (CRD) with three replicates of 33 birds per each. One group was fed with EM in 10 mL EM/L concentration level with drinking water and other group was considered as the control. Body weights of birds were measured at day 1, 10, 26 and 42 and feed intakes were measured weekly. Weight gain and feed conversion ratio (FCR) were calculated for the starter, finisher and total study period separately. At the age of 42 days, ten birds were randomly selected from each pen and scarified. Observed data were analyzed using the T-test procedure in ‘SAS’. Growth performance and FCR in starter period were significantly different (p 0.05). Total feed intake was significantly higher in EM treated birds than control group (p 0.05). In conclusion, EM positively affected the initial growth phase of broilers under local conditions though there is no significant influence on their latter part of growth.

The Influence of Effective Microorganisms on Microbes and Nutrients in Kiwifruit Planting Soil

To understand the effects of effective microorganisms (EMs) containing multiple strains on microbes and nutrients in kiwifruit planting soil, EMs prepared with four different strains were added to kiwifruit planting soil monthly from April to August. The counts of bacteria, fungi, actinomycetes, and total microbes were determined. The pH, total nitrogen (TN), alkali-hydrolyzable nitrogen (A-N), organic matter (OM), available potassium (A-K), and available phosphorus (A-P) of the soil were measured. Results indicated that the counts of bacteria, fungi, actinomycetes, and total microbes reached 60.33 × 105, 4.00 × 105, 0.92 × 105, and 65.25 × 105 CFU/g, respectively, in August, all of which were higher than those of the control group (CK). The bacterial count of the experimental group (EG) was higher than that of the CK in August. The pH-values of the EG were always lower than those of the CK. In August, the TN content of the EG was 1.52 g/kg, which was higher than that of the CK (1.35 g/kg). A significant negative association between the actinomycetes count and TN (p < 0.05) was found. For A-N and OM, the content of the EG (A-N, 125.18 mg/kg; OM, 49.84 mg/kg) was roughly the same as that of the CK (A-N, 112.51 mg/kg; OM, 53.11 mg/kg) in August. However, the A-K and A-P contents of the EG (A-K, 145.25 mg/kg; A-P, 111.25 mg/kg) were lower than those of the CK (A-K, 182.52 mg/kg; A-P, 202.19 mg/kg) in August. Results show that application of EMs in kiwifruit planting soil can increase the counts of soil microbes and might promote the absorption of major nutrients for kiwifruit tree.

The influence of effective microorganisms (EM) and yeast on the degradation of strobilurins and carboxamides in leafy vegetables monitored by LC-MS/MS and health risk assessment.

The aim of this study was to determine the behaviour of strobilurin and carbocyamides commonly used in chemical protection of lettuce depending on carefully selected effective microorganisms (EM) and yeast (Y). Additionally, the assessment of the chronic health risk during a 2-week experiment was performed. The statistical method for correlation of physico-chemical parameters and time of degradation for pesticides was applied. In this study, the concentration of azoxystrobin, boscalid, pyraclostrobin and iprodione using liquid chromatography–mass spectrometry (LC–MS/MS) in the matrix of lettuce plants was performed, and there was no case of concentration above maximum residues levels. Before harvest, four fungicides and their mixture with EM (1 % and 10 %) and/or yeast 5 % were applied. In our work, the mixtures of 1%EM + Y and 10%EM + Y both stimulated and inhibited the degradation of the tested active substances. Adding 10%EM to the test substances strongly inhibited the degradation of iprodione, and its concentration decreased by 30 %, and in the case of other test substances, the degradation was approximately 60 %. Moreover, the addition of yeast stimulated the distribution of pyraclostrobin and boscalid in lettuce leaves. The risk assessment for the pesticides ranged from 0.4 to 64.8 % on day 1, but after 14 days, it ranged from 0.0 to 20.9 % for children and adults, respectively. It indicated no risk of adverse effects following exposure to individual pesticides and their mixtures with EM and yeast.

Effective microorganisms modify protein and polyamine pools in common bean (Phaseolus vulgaris L.) plants grown under saline conditions

No information is available regarding the influence of effective microorganisms (EM) on protein synthesis and polyamine balance in plants grown under saline conditions. Thus, as a first approach, this study sheds light on some different mechanisms that may protect EM-treated plants against salt excess. The response of common bean (Phaseolus vulgaris L.) cv. Nebraska to soil salinization [0.1dSm−1 (non-saline), 2.5 and 5.0dSm−1] and/or EM application was investigated. Plants grown in saline soils exhibited a significant decline in productivity, membrane stability index, nitrate reductase activity, nitrate and protein content, K+ concentration, and K+/Na+ ratio. However, EM application ameliorated the deleterious effects of salinity and significantly improved the above parameters. Soil salinity induced oxidative damage through increased lipid peroxidation and hydrogen peroxide content. EM application significantly reduced the oxidative damage. Polyamines responded to salinity stress by increasing its content, particularly putrescine level. The EM treatment changed the polyamine balance under saline conditions, a high increase in spermidine and spermine levels was observed. Moreover, EM application significantly reduced the activities of diamine oxidase and polyamine oxidase in salt-stressed plants. Both the modulation of polyamine pool and the regulation of protein synthesis can be one of the most important mechanisms used by EM-treated plants to improve plant adaptation to saline soils.

The Influence of Effective Microorganisms on the Growth and Nitrate Content of Vegetable Transplants

The Influence of Effective Microorganisms on the Growth and Nitrate Content of Vegetable Transplants

Influence of Effective Microorganisms on Polluted Marine Sediment and Its Microbial Community

Lactobacillus sp., Acetobacter sp. and yeast were the most dominant organisms in the EM stock culture and subculture product. Lactic acid bacteria and yeast were able to grow in the fermentation process utilizing seawater. EM treatment of higher concentrations using EM stock culture and EM clay balls (1% or 4%) contributed to an early removal of malodor and an increase of DO in the polluted sediments, indicating an odor-removing activity of EM. The EM treatment of higher concentrations (1% or 4%) somewhat appeared to modify the microbial communities within the sediments, which was confirmed by existence of a few unique fragments from the stock culture based on PCR-DGGE. It still remains to be elucidated that EM cultures were directly involved in the malodor removal and potential sediment bioremediation.

Influence of “Effective Microorganisms” (EM) on Vegetable Production and Carbon Mineralization–A Preliminary Investigation

ABSTRACT The influence of effective microorganisms (EM), a commercially available microbial inoculant containing yeasts, fungi, bacteria and actinomycetes, was evaluated in field trials of commercially produced, irrigated vegetable crops on “organic” farms in Canterbury, New Zealand during 1994–1995, and in a laboratory incubation. EM plus molasses were both applied, at 10 L ha□ 1 in 10,000 L ha□ 1 water, three times to the onions, twice to the peas and seven times to the sweetcorn. EM plus molasses increased the onion yield by 29% and the proportion of highest grade onions by 76%. EM plus molasses also increased pea yields by 31% and sweetcorn cob weights by 23%. A four week incubation at 30°C of loamy sand and 1% w/w pasture litter had treatments including a control, glucose, and EM plus glucose, and captured respired carbon (C) using NaOH traps. By the end of the incubation the glucose treatment had respired 38% more C than the control. The EM treatment respired an additional 8% more C than the glucose treatment. Using EM stimulated C mineralization in the laboratory incubation, but a corresponding increase in mineralization of organic nitrogen, phosphorus and sulphur was not measured.