Influence Of Different Extracts Research Articles

Quercus infectoria gall extracts reduce quorum sensing-controlled virulence factors production and biofilm formation in Pseudomonas aeruginosa recovered from burn wounds

BackgroundQuercus gall extracts’ ability to kill pathogens in vitro and even removal of chronic drug-resistant infections has been reported by several studies. The current investigation is focused on the action of extracts of Quercus infectoria gall in their sub-inhibitory concentrations on the corresponding bacterial behaviours instead of killing them.MethodsThe effect of gall extracts on the quorum sensing (QS) associated virulence of multiple drug resistant Pseudomonas aeruginosa recovered from burns wounds was studied. The influence of different extracts on the production of bacterial virulence and biofilm, and expression of the genes encoding quorum sensing and exotoxin A were investigated. Quorum sensing is a crucial regulator of virulence and biofilm development in Pseudomonas aeruginosa and other medical related microbes.ResultsExperiments to characterise and quantify Q. infectoria gall extracts impact on the quorum sensing networks of P.aeruginosa revealed that the expression of las, rhl, and exotoxin A (ETA) genes levels including the associated virulence were reduced by the extracts at their subinhibitory concentrations.ConclusionsThe obtained results indicated that extracts of Q. infectoria galls fight infections either by their inhibitory constituents, which vigorously eradicate cells or by disruption of the pathogens quorum sensing system through weakening the virulence and bacterial coordination.

The influence of spring barley extracts on pseudomonas putida PCL1760

The aim of the present study was to evaluate the influence of different extracts (water, ethanol, enzymatic) from germinated seeds of spring barley on the development of Pseudomonas putida bacteria. The tests on optical density were performed to study the influence of different extracts on the development of model extracts. The analysis of the influence of different extracts from germinated seeds of spring barley on biological agent - Pseudomonas putida PCL1760, contained in biopreparations for crop protection showed that ethanol extract from Raushan variety of spring barley increased the resistance of Pseudomonas putida PCL1760 to the heat and osmotic stresses. All the types of extracts did not contribute to the increase of bacteria resistance to low temperatures. There was no significant difference established between the activity of spring barley extracts with vernalization and without vernalization. The materials of the study showed that ethanol extracts from spring barley can be used for the development of Pseudomonas spp. based on biopreparations for the enhancement of biocontrol microorganisms (BCMs) activity.

Screening of Complement Stimulating Activity of Lindernia madayiparense Extracts

This study evaluates the influence of different extracts of newly identified Lindernia madayiparense from Linderniaceae on complement system. The similar species under this family are used in China and India to prevent and cure diverse ailments and to rejuvenate the immune system without any marketing standards. Various extracts of plant were evaluated through complement mediated hemolytic assay. Two plant extracts shows complement modulating property against alternative pathway of complement system, i.e. ethanol extract and decocted extract. These fractions showed significant complement stimulating activity compared to positive control used.

EXTRACTS OF POPLARS BALSAMIC AS GROWTH REGULATORS OF SPRING WHEAT

The influence of different extracts obtained from leaves and buds of the poplars balsamic Populus balzamifera (water-soluble substances of the leaves, essential oil kidney and alcoholic extract of the kidneys), growing in the Krasnoyarsk territory, on the growth and development of spring wheat varieties Omskaya 36 in laboratory and field conditions is studied. Water-soluble substances leaves was isolated by the method of hot water extraction, alcoholic extract – ethyl alcohol under optimum conditions, the essential oil is by steam distillation. The obtained extracts from the vegetative parts of poplar researched generally accepted in chemistry of vegetable raw materials methods. Found that all prepared from extracts of poplar water emulsions affect the germination of seeds in laboratory conditions and on the initial growth (germination), and biological yield of spring wheat varieties Omskaya 36 in the field. The greatest positive impact on the productivity of wheat in the field had only emulsion based water-soluble substances leaves of the poplars balsamic with a concentration of 0,5 g/L. Increasing the drug concentration 10 times inhibited plant growth. Carbohydrates, amino acids and mineral components contained in the extract, presumably had a cumulative effect on seed germination and showed regulatory activity.

Influence of different extracts from persimmon leaves on the antioxidant activity in diabetic mice

To investigate the antioxidant effect of different solvent extracts from persimmon leaves (PL) in diabetic mice induced by streptozotocin (STZ). The total ethanol-extracted fraction of PL was further extracted with chloroform, ethyl acetate and n-butanol, in that order, the residues after ethanol extraction were water-extracted and alcohol-precipitated, and concentrated. The hypoglycemic effects of different solvents extracts from PL were evaluated in diabetic mice induced by STZ. The experimental mice were randomly divided into groups: control group, model group, glibenclamide group, low and high dosage groups of the various solvent extracts. The drugs were administrated to mice in every morning for 15 days. During this time period, the contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were determined. The water-extracted and ethanol-precipitated fractions and the ethyl acetate-extracted fraction markedly reduced the content of MDA and increased the activity of SOD in the livers of STZ-induced diabetic mice (P<0.01 or P<0.05). The chloroform-extracted and n-butanol-extracted fraction did not markedly reduce the content of MDA nor increase the activity of SOD in liver of STZ-induced diabetic mice (P>0.05). The ethyl acetate-extracted fraction, water-extracted and ethanol-precipitated fraction of persimmon leaves have potential value in the treatment of diabetes. The mechanism of action of the antioxidant is related to the hypoglycemic effects of extracts from persimmon leaves.

Lignicolous fungi as potential natural sources of antioxidants

As a result of an interest in natural derived metabolites around the world higher fungi (Basidiomycotina) have taken on great importance in biochemical investigations. A large number of structurally divergent compounds - both cellular components and secondary metabolites - have been extracted and found to possess significant biological activity, such as an immunomodulative effect on the human body. Effects of fungal biomolecules as potential natural antioxidants have not been examined so far. Biochemical analysis have included in vitro testing of the influence of different extracts (water methanol, chloroform) of selected fungal sporocarps on Fe2+/ascorbate-induced lipid peroxidation (LP) in a lecithin liposome system by TBA assay, as well as various other procedures. Qualitative analysis by TLC revealed a distinction both between different extracts of the same fungal species and between similar extracts of different species. The results obtained on antioxidative activities (LP inhibition and "scavenging" activity) indicate that MeOH extracts manifested a degree of activity higher than that of CHCl3 extracts with respect to antioxidative activity, the extracts can be ranged in the following declining order: Ganoderma lucidum, Ganoderma applanatum Meripilus giganteus, and Flammulina velutipes. The obtained results suggest that the analyzed fungi are of potential interest as sources of strong natural antioxidants in the food and cosmetics industries, whereas synthetic ones have proved to be carcinogenic. &lt;br&gt;&lt;br&gt;&lt;b&gt;&lt;font color="red"&gt;Detected autoplagiarism. Link to the Editorial Decision &lt;u&gt;&lt;a href="http://dx.doi.org/10.2298/ABS1004251U"&gt;10.2298/ABS1004251U&lt;/a&gt;&lt;/u&gt;&lt;/font&gt;&lt;/b&gt;&lt;br&gt;

CHEESE-RIPENING STUDIES: THE INFLUENCE OF DIFFERENT EXTRACTS ON THE ACID PRODUCTION OF LACTIC ACID BACTERIA

The influence of different extracts on the acid production of two strains of Streptococcus cremoris and two of Betacoccus cremoris isolated from Kingston cheese has been studied.The enriching entity has no effect on acid production by Streptococci in sugar broth. Enrichment with yeast or alfalfa extract causes not only a marked increase in the acid production by Betacocci, but also a definite stimulating effect on the rate of acid production.Enriching milk with yeast or alfalfa extract has a marked stimulating effect on the vital activity of the Betacocci and of Streptococcus EMB1173, but is without influence on Streptococcus EMB1195. The response of culture EMB1173 is immediate and direct, but in the case of cultures EMB2168 and EMB2173 the action of the enriching entity is cumulative in its effect. Alfalfa extract would appear to provide, in the case of Betacoccus EMB2173, a stimulating influence not to be found in yeast extract.The influence of other forage crop enrichments on rate of acid production has also been studied. The factor or factors present in alfalfa and shown to exert a stimulating influence on the vital activity of the organisms are to be found to some extent in all forage crops investigated.