This research aimed to know the influence of application technique and biofertilizer that given concentration of biosurfactant diethanolamide (DEA) for the growth of cocoa seedlings and also to know the interaction between application technique and concentration level of biofertilizer given DEA growth of cacao seedling (Theobroma cacao L.). This research was conducted in UPTD SITANDU, Banten and the Soil Agroclimate Laboratory, Faculty of Agriculture, University of Sultan Ageng Tirtayasa from December 2020 until March 2021, by using randomize complete block design with two factors. The first factor is technique application T1 at soil, T2 at leaf, and T3 with combination soil and leaf fertilization), and the second factor is concentration level of biofertilizer given DEA P1 10 ml and 5%, P2 10 ml and 10%, P3 20 ml and 5%, P4 20 ml and 10%. As a result, there were twelve possible treatment combinations, and each was applied three times. Parameters total chlorophyll (unit), root length (cm), stem diameter (mm), seedlings dry weight (g) and root shoot rasio (g). The results there was no interaction effect between the two treatments, namely application technique and concentration level of biofertilizer with DEA biosurfactant, on all observed parameters of cocoa seedlings.
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