Infertility Problems Research Articles

Determining effective waste human hair hydrolyzing parameters combination and its typical physicochemical characteristics in synthesizing liquid nitrogenous organic fertilizer

Discarded Human hairs are considered as waste material in most parts of the world and its accumulation causing several environmental problems. However as a potential material resource, human hair has the advantage that it is completely biodegradable, renewable and available in every locality. Thus reutilizing human hair cut discards with a target of extracting a particular major nutrient supply, namely nitrogen, carbon, sulfur and so forth, can create a positive impact on solving soil’s infertility problems and less productivity issues of concerned agrarian communities. This study has tried to develop waste human hair based bio-fertilizer by primarily identify the better performing hydrolyzing mixture rang of formulation (H5), ratio of human hair waste mass to aqueous hydrolyzer mixture usages (1:5), favorable reacting time ( 90 min) and temperature ( 70 oC) values progressively. Then the physicochemical characteristics were analyzed to reveal the resulted final product’s viscosity (~ 76.0 cp.), density (~ 1.17 gm/l), pH (~ pH 9), organic nutrients availability concerning primary Macronutrients (~ 18:0.03:1 N-P-K ratio ), Secondary Macronutrients (~ 0.13:0:7 Ca-Mg-S ratio) and other trace micronutrient constituents; which are considered as requirements for the soil nutrient enrichments and plant growths.

The function of the endocrine system in women with infertility

Infertility is the inability of a couple of childbearing age to conceive a child within 1 year with regular unprotected sexual activity. In the modern world, the problem of infertility is felt very acutely. According to statistics, from 17 to 25% of couples are infertile, the indicators depend on the region. ART (assisted reproductive technologies) in the modern world often become the only possible solution for an infertile couple. In recent years, they have been resorted to much more often and this trend will clearly continue to be observed. Over the past 10 years, the number of women who have undergone embryo transplantation in the Department of Assisted Reproductive Technologies of the MRCCB Perinatal Center has increased from 203 to 501. Infertility of endocrine genesis is one of the main diagnoses with which patients seek the help of assisted reproductive technologies.

Overexpression of TAF4B Promoted the Proliferation of Undifferentiated Spermatogonia in Cattleyak InVitro.

As the hybrid between cattle and yak, cattleyak is a typical male sterile mammal, and the underlying mechanism for its spermatogenic arrest is still unclear. In this study, the coding region of cattleyak TAF4B gene was cloned by RT-PCR and analysed by bioinformatics. To investigate the effects of TAF4B on cellular proliferation and differentiation, an expression vector was generated and introduced into undifferentiated spermatogonia (UDSPG) of cattleyak. The results showed that the protein encoded by TAF4B did not contain the signal peptide sequence. The expression level of TAF4B in UDSPG of cattleyak was lower than that in yak, while the overexpression of TAF4B in cattleyak promoted the proliferation activity of cattleyak UDSPG. Meanwhile, the expression of proliferation and meiosis-related genes was increased but the differentiation-related genes were decreased. Therefore, the aberrant expression of TAF4B in cattleyak UDSPG possibly impaired its proliferation and differentiation equilibrium and decreased its growth potentiality, thereby reducing the quantity of UDSPG and affecting spermatogenesis. This study provided a potential approach for further elucidation of the mechanism of spermatogenesis arrest and provided a new idea for solving the problem of male infertility in cattleyak.

ЛЕЧЕБНО-ВОССТАНОВИТЕЛЬНЫЕ И БИОТЕХНОЛОГИЧЕСКИЕ РЕСУРСЫ НАУЧНОЙ МУЗЫКОТЕРАПИИ В РЕШЕНИИ ПРОБЛЕМ БЕСПЛОДИЯ

Scientific music therapy is an advanced therapeutic and preventive direction, widely in demand in various areas of health care, psychological assistance, and social rehabilitation, but still underestimated in reproductive medicine. Complex scientific research conducted over thirty years has helped to better understand the nature of the main mechanisms of music's influence on the neuroendocrine system,vital organs, and cellular structures. On this basis, unique and effective methods of scientific music therapy and promising biotechnologies have been created, the possibilities of which in solving infertility problems are discussed in this article.

Anomaly Detection in Embryo Development and Morphology Using Medical Computer Vision-Aided Swin Transformer with Boosted Dipper-Throated Optimization Algorithm.

Infertility affects a significant number of humans. A supported reproduction technology was verified to ease infertility problems. In vitro fertilization (IVF) is one of the best choices, and its success relies on the preference for a higher-quality embryo for transmission. These have been normally completed physically by testing embryos in a microscope. The traditional morphological calculation of embryos shows predictable disadvantages, including effort- and time-consuming and expected risks of bias related to individual estimations completed by specific embryologists. Different computer vision (CV) and artificial intelligence (AI) techniques and devices have been recently applied in fertility hospitals to improve efficacy. AI addresses the imitation of intellectual performance and the capability of technologies to simulate cognitive learning, thinking, and problem-solving typically related to humans. Deep learning (DL) and machine learning (ML) are advanced AI algorithms in various fields and are considered the main algorithms for future human assistant technology. This study presents an Embryo Development and Morphology Using a Computer Vision-Aided Swin Transformer with a Boosted Dipper-Throated Optimization (EDMCV-STBDTO) technique. The EDMCV-STBDTO technique aims to accurately and efficiently detect embryo development, which is critical for improving fertility treatments and advancing developmental biology using medical CV techniques. Primarily, the EDMCV-STBDTO method performs image preprocessing using a bilateral filter (BF) model to remove the noise. Next, the swin transformer method is implemented for the feature extraction technique. The EDMCV-STBDTO model employs the variational autoencoder (VAE) method to classify human embryo development. Finally, the hyperparameter selection of the VAE method is implemented using the boosted dipper-throated optimization (BDTO) technique. The efficiency of the EDMCV-STBDTO method is validated by comprehensive studies using a benchmark dataset. The experimental result shows that the EDMCV-STBDTO method performs better than the recent techniques.

Sexual function in women with unexplained infertility

Background. The problem of infertility, including unexplained infertility (UEI), does not lose its relevance. It is known that infertile women are prone to emotional disorders. Such psychological changes can contribute to the development of sexual dysfunction. Aim. To study the characteristics of sexual function in women with unexplained infertility. Materials and methods. A comparative study of sexual function was conducted in 60 women with UEI aged 20–40 years (group 1). Group 2 consisted of 75 fertile women with at least one living healthy child and no history of infertility. Group 3 included 75 healthy nulliparous women who do not plan pregnancy and do not have a diagnosis of “Infertility”. The questionnaire “Index of Female Sexual Function” (FSFI) was used to assess sexual function. The following parameters were assessed: “desire”, “arousal”, “lubrication”, “orgasm”, “satisfaction”, “pain”, and the final score. Results. The lowest values were in group 2. In this group, statistically significant differences were obtained with groups 1 and 3 in the parameters “Attraction”, “Arousal”, “Lubrication”, “Satisfaction”, “Orgasm”. In group 1, the final score was significantly higher than in groups 2 and 3 – 30.2 (26.3–31.7), 26.6 (23.7–28.8) and 28.3 (27.5–29.9); p0.001, respectively. The highest proportion of women with sexual dysfunction (FSFI26.55) was in group 2 – 37 (49.33%). In groups 1 and 3, the number of women with sexual dysfunction was 13 (21.66%) and 9 (12%), respectively (the differences between groups 1 and 3 were not significant). Conclusion. The quality of sexual function was not associated with reproductive disorders. The lowest rates were in group 2. This is probably due to psychological aspects and anatomical changes in the pelvic floor after childbirth. Many women, regardless of the presence of children, need therapeutic and preventive sexological care. In the case of sexual dysfunctions that impair a woman's quality of life, qualified sexological and psychological assistance should be provided regardless of her fertility.

Modern concepts about the problem of endometrial infertility against the backdrop of the "thin endometrium" (literature review)

Endometrial infertility is the cause of inadequate implantation and serves as an obstacle to the onset and progression of spontaneous pregnancy and the implementation of assisted reproductive technology (ART) programs. One of the most common mechanisms for the development of infertility is endometrial thinning. Despite the abundance of publications, today there are no generally accepted diagnostic criteria and treatment and rehabilitation approaches for this pathology, which encourages continued scientific research in this direction. Aim was to highlight the problem of decreased fertility caused by “thin endometrium”. To create the review, the bibliographic method was applied and domestic and foreign literary sources from the Pubmed, MedLine, RSCI, e-library and Cyberleninka search engine databases were used. The search depth was 10 years. The following search phrases were used: “uterine factor of infertility”, “endometrial factor of infertility”, “thin endometrium”. Seventy five sources that were included in this review were studied and analyzed. Data presented in the literature indicate that endometrial causes of infertility are associated with incompetence of the uterine cavity mucosa, which may manifest itself as a phenomenon of ”thin endometrium”, the development mechanisms of which have not yet been fully studied. Determining the functional status of the endometrium is very important for predicting the onset of pregnancy in the natural cycle or in ART programs, since even in the presence of a good quality embryo, endometrial deficiency can prevent adequate implantation. Thus, before planning a subsequent pregnancy, it is necessary to diagnose and, if possible, neutralize or correct endometrial causes of infertility. One of the main markers of readiness for implantation at the moment is the thickness of the endometrium.

Comparative Characteristic of the Methods of Isolation of Oocytes and Spermatozoa in Laboratory Animals (Review).

Over the past decade, there has been an increasing trend in the use of assisted reproductive technologies, which have significantly expanded the opportunities to overcome the problem of infertility. However, the problem of increasing the effectiveness of in vitro fertilization remains open. Isolation of germ cells from animals is a necessary process for various experimental studies. Animal germ cells can be used in experiments to study physical, chemical, genetic, immunological, and microbiological factors affecting reproduction efficiency and for the development of techniques that increase the effectiveness of in vitro fertilization. All of the above determines the relevance of studying existing methods of oocyte and sperm isolation for experimental in vitro studies. Here we discuss the existing methods of sperm and oocyte isolation from animals and their advantages and disadvantages, and also substantiate priority methods for use.

EFFECT OF PHYSIOTHERAPY ON THE IMPLANTATION POTENTIAL OF THIN ENDOMETRIUM

The article is dedicated to the urgent problem of infertility associated with thin endometrium. The mechanisms of action of various physiotherapeutic methods on the endometrium, such as magnetotherapy, laser therapy, ultrasound therapy, and electrical stimulation, are discussed. The beneficial effects of physiotherapy are described in detail, including improved blood flow, reduced inflammation, and stimulation of tissue regeneration. The results of clinical trials confirming the effectiveness of physiotherapy in increasing the thickness and improving the structure of the endometrium are presented. The article also includes indications and contraindications for the use of physiotherapy and emphasizes the importance of an individualized approach to treatment.

Correlation of Recombinant FSH Doses with Follicular and Serum Level Resolvin E1 in Polycystic Ovarian Syndrome Women Undergoing ICSI

Background: Reproduction is a intricately structured process achieved by interaction of the higher centers in the brain, the gonads, and different body parts; Whereas infertility is a multifactorial pathological condition affecting approximately 10–15% of couples, especially in industrialized region.RESOLVIN E1 is a member of specialized pro resolving mediator family of bio active lipids. It has anti-inflammatory effects in various inflammatory diseases. A promising researches in their way to prove its positive role in reproduction through direct effect on folliculogenesis. It has a fundamental role in regulating follicular growth and maturation at central and peripheral levels highlighting its possible role in diagnosing and treating infertility problems. High level of RESOLVIN E1 which might play critical role in poly cystic ovarian syndrome due to its anti-inflammatory effects. Objectives: To investigate the correlation of serum and follicular RESOLVIN E1 to gonadotropin (recombinant FSH doses) in PCOS patient undergoing ICSI. Subject, Patients, Materials, and Method: A prospective cross-sectional study conducted in the high institute for infertility diagnosis and assisted reproductive technology /Al Nahrain University /Baghdad /Iraq, from November 2023 till March 2024. Twenty-nine infertile PCOS women who were undergoing intracytoplasmic sperm injection were enrolled in the study. Serum and follicular RESOLVIN E1 at day of oocyte pickup were measured using ELISA technique. Results: The current study shows significantly lower gonadotropins stimulation dose needed for PCOS females (p <0.001). It also revealed no significant correlations between total gonadotropins dose with both serum and follicular fluids resolvin E1, while there were insignificant negative correlations between serum and follicular fluids resolvin E1 with patients’ ages and body mass indices among PCOS females. Conclusions: There is no correlation between the serum and follicular RvE1 levels in PCOS infertile females.

AYURVEDIC MANAGEMENT OF KSHEENA SHUKRA (OLIGOSPERMIA) LEAD-ING TO MALE INFERTILITY: A CASE STUDY

As per the World Health Organization (WHO) report in India, the overall prevalence of primary infertility ranges between 3.9 and 16.8%. A recent report on the status of infertility states that 50% of it is related to productive anomalies or disorders in males. In comparison, 90% of male infertility problems are related to Oligospermia and other abnormalities in semen analysis. As per Ayurveda, Oligospermia can be considered as Ksheena Shukra (Oligospermia). Surgery, Hormone treatments, medications and Assisted Reproductive Technology (ART) are the main lines of treatment in Conventional medicine, but these are associated with many unwanted and severe adverse effects. The present case discussion is about effectively managing a case diagnosed as Ksheena Shukra with Ayurvedic intervention. This case report aims to evaluate the effect of Ayurvedic treatment in managing Ksheena Shukra. In this case study, Shodhana (Purificatory Therapy) procedures {especially Virechana Karma (Purgation) and Vasti(Enema)} were performed before administering Rasayana (Rejuvenation) drugs; the pre- and postsemen analysis during the treatment period shows a marked increase in sperm count and motility. The outcome of this case reveals that Ayurvedic treatments can effectively manage the Oligospermia associated with male infertility.

Recent scientific data on the treatment of female infertility of tubal origin

In 1976 in the United States about 10-15% of couples failed to conceive after at least one year of marriage without the use of contraceptive methods. At the same time, the number of visits to private doctors for infertility problems increased from about 600,000 in 1968 to about 900,000 in 1980. This increased concern about infertility problems appears to be concentrated in that cohort of the American female population born after World War II, currently in their thirties or early forties.

Effect of Myo-Inositol in Treating Polycystic Ovary Syndrome (PCOS): A Review

Introduction: Background: Women of reproductive age are often affected by Polycystic Ovary Syndrome (PCOS), a condition that can cause infertility and metabolic problems. Hormonal changes contribute to PCOS's mechanism. It involves three interrelated symptoms, namely ovulation disorders, androgen excesses, and Polycystic Ovarian Morphology (PCOM), which should all be treated appropriately. Inositol therapy has been shown to play a significant role in PCOS in several studies. Despite this, there is no comprehensive discussion of Myo-Inositol (MI) and D-Chiro-Inositol (DCI) in relation to particular symptoms. Aim: The purpose of this review is to demonstrate how well Myo-inositol treats PCOS symptoms. Additionally, the study emphasises on evaluating inositol consumption while considering their physiological characteristics and the process by which certain PCOS symptoms arise. Methods: Using the databases PubMed and Google Scholar, a review of the literature was carried out using one of the following keywords: PCOS, myo-inositol, and insulin resistance. Results: Multiple research studies have shown that the treatment of MI improved the function of ovaries and fertility in patients with Polycystic Ovarian Syndrome (PCOS), reduced symptoms of hyperandrogenism, including acne and hirsutism, beneficially affected metabolic aspects, and regulated a number of hormonal factors that are deeply connected to the function of the reproductive system and ovulation. Thus, using MI as a treatment has become a breakthrough approach to enhance spontaneous ovulation, stimulate ovulation, or minimise PCOS symptoms. Conclusion: A physiological ratio of 40:1 between MI and DCI could prove to be advantageous for addressing the metabolic, hormonal, and reproductive components of PCOS, according to the existing clinical evidence.

A Study of Psychological Distress Among Women with Polycystic Ovarian Syndrome in Kashmir

Background: Polycystic Ovarian Syndrome, was previously thought to be an endocrine condition. Research has now demonstrated that polycystic ovarian syndrome affects a patient's quality of life and is a metabolic, hormonal and psychosocial condition. Women's joys are disrupted by PCOS because the stigma attached to hyperandrogenism is strongly felt and may harm women's psychological development. Methods: The aim of the study was to comprehend the psychosocial problems experienced by women in Kashmir and to obtain a thorough understanding of the coping mechanisms employed by women with PCOS. The research design was both qualitative and quantitative in nature. Thematic analysis was used to derive themes from the data. Sampling was purposeful in nature. Results: The study underscores that women with PCOS encounter numerous difficulties encompassing social and psychological aspects. Psychological issues like mood swings, stress, anxiety and sleeping disorders are prevalent among women with PCOS. Furthermore, the research has revealed that menstrual disorders significantly contribute to depression in women. The study also highlights a connection between infertility and psychological problems. Conclusion: In conclusion, the research underscores the multifaceted challenges faced by individuals with PCOS. Therefore, understanding these issues is crucial for developing effective coping mechanisms and improving the quality of life for women dealing with PCOS.

БЕРЕМЕННОСТЬ, РОДЫ И ПЕРИНАТАЛЬНЫЕ ИСХОДЫ У БЕРЕМЕННЫХ ПОСЛЕ ЭКСТРАКОРПОРАЛЬНОГО ОПЛОДОТВОРЕНИЯ

Almost every day we face the problem of infertility among married couples. It should also be noted that currently male and female infertility occur with approximately the same frequency. According to information provided by the World Health Organization, one in six people in the world faces the problem of infertility. Assisted reproductive technologies for the treatment of infertile couples are considered a serious medical intervention worldwide. The updated data confirmed the absence of significant differences in the prevalence of infertility among different regions of the world. Thus, high-, middle- and low-income countries have comparable infertility rates. This means that this problem is a worldwide health problem. At the same time, there are significant differences in the availability, quality and provision of infertility treatment services between high-income and low-income countries. The purpose of the study: to study the features of the course of pregnancy, childbirth and perinatal losses in pregnant women after in vitro fertilization. Materials and methods of research. A retrospective analysis of the course of pregnancies, childbirth and perinatal outcomes was performed in 155 patients whose pregnancy occurred after the use of assisted reproductive technologies (in vitro fertilization). Only women whose pregnancy ended in childbirth were included in the study. Results. Further clinical studies are needed to reduce the incidence of pregnancy complications and improve perinatal outcomes. In addition, it is necessary to expand the availability of all necessary low-cost and high-quality methods of infertility treatment.

Impact of pre-conception diet on female infertility

Introduction and purpose Infertility is defined as a condition characterized by the inability to become clinically pregnant after 12 months of regular, unprotected sexual intercourse, or because a person's ability to reproduce, either as an individual or with his or her partner, is impaired. Infertility affects between 8% and 12% of couples of reproductive age worldwide. Fertility decline in women begins as early as age 25-30. The purpose of this article is to present the problem of infertility in women, possible diagnostic options, methods of prevention and treatment of infertility. We draw attention to the important role of a proper lifestyle, including diet in the fight against infertility. State of knowledge The article reviews the literature on infertility with special attention to the problem of infertility in women. Knowing the causes of infertility, it is possible to implement targeted diagnosis and then treatment. Attention is paid to the influence of proper diet on female fertility. Material and method A review of the literature available on PubMed and Google Schoolar was conducted. We focused on presenting different causes of infertility in women, diagnostic options, treatment methods depending on the cause of the problem. We paid special attention to proper diet and its impact on female fertility. Summary In our review, we presented how big a problem female infertility is. We managed to describe the current state of knowledge on the definition, epidemiology of fertility and infertility, and the main causes of female infertility. Based on a number of studies, we have proven the beneficial effects of a proper lifestyle with emphasis on a proper diet.

Dual Jeopardy: Managing Vaginismus in a Female Having a Partner Suffering from Psychotic Disorder

Vaginismus is a complex condition to manage, and in the presence of a partner, management becomes more complex as it involves the engagement of both partners. Besides addressing vaginismus, the management may involve addressing additional issues such as marital disharmony, situational erectile dysfunction in the partner, infertility, mental morbidity, and other associated psychosocial problems. There is a lack of data on the management of vaginismus in a woman with a partner with a psychotic disorder. In this report, we describe a couple in which the female partner had vaginismus that led to a lack of consummation of marriage for six years, and the male partner had schizophrenia. We discuss the challenges faced in the successful management of vaginismus by using an eclectic approach.

The role of infertility-related stress and sexist beliefs in anxiety symptoms in infertility couples

ABSTRACT It is necessary to continue identifying the predictors of anxiety symptoms in men and women undergoing assisted reproductive technology (ART), especially from a psychosocial perspective. For this reason, this study evaluated a mediation model that demonstrates the effect of various stressors related to infertility, as well as the variables of sex, age, social support, and sexist beliefs, on anxiety symptoms. The sample of this cross-sectional study consisted of 215 infertility patients (146 women and 69 men) undergoing ART and residing in Spain. The Inventory of Infertility Problems (FPI) was used, which measures five stressors related to infertility (social, sexual and relationship concerns, rejection of a child-free lifestyle and need for parenthood). The Hospital Anxiety and Depression Scale (HADS) and the Ambivalent Sexism Inventory (ASI) were also used. Being women and perceiving less social support was associated with higher anxiety symptoms, due to the mediated effect of social, sexual and relationships concerns. Higher levels of sexist beliefs were related to greater concern about the relationship, greater rejection of a childless lifestyle and greater need for parenting. This pioneering research in Spain identifies factors predisposing to anxiety in ART patients. This evidence should be considered in infertility research and clinical practice.

Innovative methods for solving the problem of female infertility associated with endocrine disorders

Aim: to analyze the publications assessing innovative methods of diagnostics and treatment used to solve the problem of female infertility associated with endocrine disorders.Materials and Methods. There was conducted a search for publications in electronic databases PubMed, Google Scholar, and eLibrary, which were selected in accordance with the PRISMA guidelines. All relevant articles published up to January 2024 were included in thereview. As a result, 374 publications retrieved from PubMed, 147 – from eLibrary, and 33– from Google Scholar were extracted. Duplicates and non-full-text versions of articles were excluded. After the selection procedure, 72 publications were included in the review.Results. During the data analysis, we identified key aspects providing deeper understanding of endocrine disorders affecting female infertility. In particular, new correlations were demonstrated between the level of select hormones and treatment success, as well as pathogenetic mechanisms were also identified influencing the conception and pregnancy process. These results will allow to develop more accurate diagnostic criteria and effective treatment methods able to increase women's chances of successful conception and pregnancy.Conclusion. The data analysis identified the hallmark aspects for diagnostics and treatment of female endocrine infertility, with new diagnostic criteria and effective treatment methods identified able to markedly improve outcomes. The crucial potential of the innovative approaches and methods considered contributes to improving reproductive health and increasing the chances of successful conception. We strongly encourage the introduction of such advanced technologies into clinical practice to optimize reproductive success.

Exploring the lived experiences of women with infertility using traditional healthcare services in Harare urban, Zimbabwe.

Infertility has a significant impact on the lives of women. Therefore, affected women often consider the treatment options available to deal with their condition, including traditional healthcare services (THS). The aim of this phenomenological study was to explore the lived experiences of women with infertility problems who sought help from traditional health practitioners in Harare, Zimbabwe. Data from interviews with five women with infertility was explicated using a simplified version of Hycner (1985) five step explication process. Two major themes and eight sub themes emerged from the findings. The major themes were traditional diagnosis experiences and traditional treatment experiences. Consultation and divination were the diagnosis methods experienced by the women with infertility. The THS offered comprehensive management of infertility through couples therapy, as well as pre- and post-natal therapies, which include lifestyle counselling. The findings also showed that women with infertility commonly receive concurrent treatment, including both allopathic and traditional medicine. This presents an opportunity to explore the convergence of traditional and allopathic approaches in the management of infertility in women.