Our study was conducted to detect virulence genes in Serratia marcescens. It has many virulence genes that cause nosocomal infections in immunocompromised persons and neonates. A total of 24/100 (24%) S. marcescens were obtained from neonates suffering from meningitis, and they were identified using culture characteristics biochemical- tests and confirmed by Polymerase chain reaction (PCR) technique, using the 16S rRNA gene. All virulence factors, including the fimA gene that encodes type-1 fimbria, the bsmB gene that encodes exo polysaccharide production, and ampC that encodes ß-lactamase enzymes, were done using the PCR technique. The results revealed that S. marcescens isolates have 16S rRNA gene at the percentage (100%), fimA gene at the percentage (54%), bsmB gene at the percentage (71%) and ampC gene at the percentage (100%). Finally, the DNA sequencing of (fimA, bsmB, and ampC genes) was done using a DNA sequencer technique to determine the sequence of nucleotides. The results revealed the similarities of the genes in local isolates of S. marcescens (98%) with S. marcescens isolates globally registered on the NCBI-Genbank website. Keywords: fimA gene, bsmB gene, ampC gene, Serratia marcescens, DNA sequences.