Inexpensive Setup Research Articles

Comprehensive Analysis of Parametric Effects on the Specific Heat Capacity of Pristine and Aged Lithium-Ion Cells

The precise determination of the specific heat capacity of lithium-ion cells is essential for thermal management design. Though, varying influences and insufficient parametric analyses are found in the literature. Therefore, a simple and inexpensive measurement setup is utilized to measure the specific heat capacity of cells independent of their format and dimensions. A comprehensive parametric analysis is performed assessing the effect of cell casing, cell chemistry, temperature, state-of-charge, and state-of-health. For the first time ever, a predictive analysis on material level is conducted allowing for understanding the individual factors in detail. Thus, an analytical approach for calculating the specific heat capacity can be validated by comparing predictive values to experimental data for the first time. It is found that the cell format has a significant influence on the specific heat capacity due to varying mass fractions and housing materials. Furthermore, the cell chemistry and corresponding layer thicknesses are of high importance, too. By selecting specific heat capacities for individual materials from the general literature, the analytical prediction matches the experimental data and is thus validated for the first time ever. Moreover, temperature has a positive linear effect on the specific heat capacity which can increase by up to 15% over the operating range. Furthermore, the positive temperature dependency improves the charging performance. Finally, neither SOC nor SOH significantly affect the specific heat capacity of lithium-ion cells.

Cellular behavior analysis from live-cell imaging of TCR T cell-cancer cell interactions.

T cell therapies, such as chimeric antigen receptor (CAR) T cells and T cell receptor (TCR) T cells, are a growing class of anti-cancer treatments. However, expansion to novel indications and beyond last-line treatment requires engineering cells' dynamic population behaviors. Here we develop the tools for cellular behavior analysis of T cells from live-cell imaging, a common and inexpensive experimental setup used to evaluate engineered T cells. We first develop a state-of-the-art segmentation and tracking pipeline, Caliban , based on human-in-the-loop deep learning. We then build the Occident pipeline to collect a catalog of phenotypes that characterize cell populations, morphology, movement, and interactions in co-cultures of modified T cells and antigen-presenting tumor cells. We use Caliban and Occident to interrogate how interactions between T cells and cancer cells differ when beneficial knock-outs of RASA2 and CUL5 are introduced into TCR T cells. We apply spatiotemporal models to quantify T cell recruitment and proliferation after interactions with cancer cells. We discover that, compared to a safe harbor knockout control, RASA2 knockout T cells have longer interaction times with cancer cells leading to greater T cell activation and killing efficacy, while CUL5 knockout T cells have increased proliferation rates leading to greater numbers of T cells for hunting. Together, segmentation and tracking from Caliban and phenotype quantification from Occident enable cellular behavior analysis to better engineer T cell therapies for improved cancer treatment.

Experimental studies on dynamic response of piezoelectric based hemispherical resonator gyroscope

PurposeThis work measures the performance characteristics of a hemispherical resonator gyroscope (HRG) and compares it with a numerical model.Design/methodology/approachThis work we explore the optical and piezoelectric measurement methods to determine the resonant frequency of HRG. These experimental results are compared with their numerically obtained values. To explore the performance characteristics, the effect of varying actuation voltages on the sense mode displacement and the piezoelectric sensor output was studied in the absence of input angular rate. The structure was then subjected to range of angular rate signals, at a constant actuation voltage and the corresponding sensor response was analysed.FindingsExperimental values of the resonant frequencies in drive and sense modes are found to be within 8% of the numerical results. The sensor output depicts a quadratic dependency on the applied angular rate, which is synchronous with the governing equations of the HRG. The experimental output is within 12% of that obtained numerically. The sensor is found to resolve upto 0.24 rad/s.Originality/valueThis work presents an in-house developed inexpensive measurement setup for static and dynamic characterization of mesoscale MEMS gyroscopes. The measurement setup can also be modified accordingly for measurement of other MEMS-based devices.

Preparation of low-concentration H2 test gas mixtures in ambient air for calibration of H2 sensors

Abstract. Using electrochemical gas sensors for quantitative measurements of trace gas components in ambient air introduces several challenges, of which interference, drift and aging of the sensor are the most significant. Frequent and precise calibration as well as thorough characterization of the sensor helps to achieve reliable and repeatable results. We therefore propose the use of a simple, lightweight and inexpensive setup to produce hydrogen calibration gases with precisely known concentrations in ambient air. The hydrogen is produced by electrolysis with electric current monitoring, and the output can be set to any value between ∼ 3 and ∼ 11 µgH2min-1. With a dilution flow of 500 mL min−1, for example, this results in a concentration range from ∼ 70 up to ∼ 240 ppm, but concentrations significantly below or above this range can also be covered with accordingly modified dilution flows. This setup can be used not only for calibration, but also for thorough and long-term characterization of electrochemical gas sensors to evaluate sensitivity, zero voltage and response time over extended periods of time.

A comparative study of the effectiveness of photogrammetric versus manual anthropometric measurements.

The accurate measurement of the human body is essential when it comes to designing agricultural tools and equipment that can effectively accommodate and interact with individuals when performing a task. The traditional method for measuring an individual's body measurements is highly complex and requires two or more skilled individuals and reliable measurement tools. Finding a new approach that is speedier, more precise, and less expensive than current methods is therefore necessary. This study aims to develop an inexpensive novel photogrammetric anthropometric measurement setup that can extract the dimensions of an individual subject irrespective of their body shape. This study involved the creation of a setup comprising four cameras for a 360° photoshoot of human subjects to calibrate and test the developed measurement setup for capturing photos of human subjects and compare the results with manual measurements. Ten different body dimensions were measured using the setup. There was a significant correlation between the manual and photogrammetric measurement methods (0.943 < r < 0.997). The highest absolute error recorded was 1.87%. The photogrammetric method for collecting anthropometric data is a reliable substitute for manual measurements across diverse populations. The results indicate that this low-cost approach is highly precise and reliable, with strong correlation to manual measurements. Multiview photogrammetry proves effective for individuals of various body shapes, making it a versatile option for data collection.

Electrochemically‐Enabled Construction of N‐Acyl Iminophosphoranes

AbstractN‐acyl iminophosphoranes have shown significant potentials in various areas. This paper presents two electrochemical strategies for the successful construction of a series of substituted N‐acyl iminophosphoranes using electrochemical activation of hydroxylamines or dioxazolones to generate unprotected amide radicals and radical anions. The simple undivided cell with inexpensive electrode setups (graphite felts, carbon rods, and copper sheets) provides excellent substrates tolerance under mild conditions. This simple electrochemical construction of N‐acyl iminophosphoranes also has good scalability, practicability and extensibility. Furthermore, synthetic transformations of the N‐acyl iminophosphorane products have demonstrated the application potentials of the P=N bond.

Inverse design from the catenary problem

Inverse problems in science normally involve the challenge of obtaining from a set of observations the causal factors that generated them in the first place. However, physics students are seldom exposed to such problems as part of their training. Here we revisit the mechanics problem of finding the shape of a hanging cable, but this time in reverse, i.e. by asking what mass density a cable must have to follow a specific shape. This concept is then generalised into the possibility of identifying a cable whose hanging shape follows any form we wish to design. This inverted design strategy is experimentally verified with an inexpensive setup that is suitable for classroom activities.

Simple and inexpensive microwave setup for industrial based applications: Quantification of flower honey adulteration as a case study.

A simple and inexpensive microwave measurement setup based on measurements of magnitudes of transmission properties ( ) is proposed for industrial-based microwave aquametry (moisture or water content) applications. An easy-to-apply calibration procedure based on normalization is implemented to eliminate systematic errors in the measurement system. As a case study, we applied this setup for the quantification of water-adulteration in flower honey. After validating this system by distilled water and pure flower honey measurements, measurements of the pure flower honey with various adulteration percentages ( ) up to 9% are conducted to examine the performance of the measurement setup for quantification of water adulteration. A multi-dimensional fitting procedure is implemented to predict using the proposed inexpensive microwave measurement setup. It is shown that it is possible to quantify an adulteration level with an accuracy better than % by the proposed measurement setup and the applied multi-dimensional fitting procedure.

A 3D-Printed and Freely Available Device to Measure the Zebrafish Optokinetic Response Before and After Injury.

Zebrafish eyes are anatomically similar to humans and have a higher percentage of cone photoreceptors more akin to humans than most rodent models, making them a beneficial model organism for studying vision. However, zebrafish are different in that they can regenerate their optic nerve after injury, which most other animals cannot. Vision in zebrafish and many other vertebrate animals, including humans, can be accessed using the optokinetic response (OKR), which is an innate eye movement that occurs when tracking an object. Because fish cannot use an eye chart, we utilize the OKR that is present in virtually all vertebrates to determine if a zebrafish has vision. To this end, we have developed an inexpensive OKR setup that uses 3D-printed and off-the-shelf parts. This setup has been designed and used by undergraduate researchers and is also scalable to a classroom laboratory setup. We demonstrate that this setup is fully functional for assessing the OKR, and we use it to illustrate the return of the OKR following optic nerve injury in adult zebrafish.

An Immersive Hybrid Approach to Materials &amp; Solid Mechanics Lab Activities for Undergrad Students

This paper describes two institutions' efforts to provide engineering science students with experiential learning opportunities using low-cost, simple physical lab experiments and its efficacy in improving students perceived understanding levels. A Canadian university developed a "hybrid" lab activity that combined a virtual lab simulator with physical lab experiments to teach materials, solid mechanics, and instrumentation concepts in two different 2nd year undergraduate solid mechanics courses. At a small American college, students in an introduction to materials engineering course completed four individual laboratory exercises using simple and relatively inexpensive material testing setups that explored topics covered in course lectures and readings. Students learned about the behavior of engineering materials and structural analysis using low-cost materials test apparatuses for different loading modes, engaging their senses to aid their understanding. Students then constructed a virtual simulation model using Finite Element Analysis (FEA). Students at both institutions gave positive feedback and reported improved understanding of course topics. The use of low-cost experiments combined with traditional engineering labs shows promise for improving student understanding of engineering science concepts.

Construction and remote demonstration of an inexpensive but efficient experimental setup for studying self-inductance and mutual-inductance between two coils

Measurement of self-inductance and mutual-inductance has been demonstrated as a laboratory experiment with great simplicity. The novelty of this demonstration lies in its straightforward circuitry and methodology, enabling the experiment accessible to students with minimal prerequisite knowledge. A coil-pair setup was constructed using discarded common household articles, costing very low but provides high quality throughput in teaching-learning experience. The demonstration can be easily conducted online using a laptop without the need of extra power supply. The experiment suits for high school and pre-university students, as evident by twenty first-semester undergraduate physics and electronics students who found it both satisfying and enjoyable with a relatively flat learning curve.

Continuous Renal Replacement Therapy During Long-term Normothermic Machine Perfusion of Human Donor Livers for up to 7 D.

Normothermic machine perfusion (NMP) is used to preserve and test donor livers before transplantation. During NMP, the liver is metabolically active and produces waste products, which are released into the perfusate. In this study, we describe our simplified and inexpensive setup that integrates continuous renal replacement therapy (CRRT) with NMP for up to 7 d. We also investigated if the ultrafiltrate could be used for monitoring perfusate concentrations of small molecules such as glucose and lactate. Perfusate composition (urea, osmolarity, sodium, potassium, chloride, calcium, magnesium, phosphate, glucose, and lactate) was analyzed from 56 human NMP procedures without CRRT. Next, in 6 discarded human donor livers, CRRT was performed during NMP by integrating a small dialysis filter (0.2 m2) into the circuit to achieve continuous ultrafiltration combined with continuous fluid substitution for up to 7 d. Within a few hours of NMP without CRRT, a linear increase in osmolarity and concentrations of urea and phosphate to supraphysiological levels was observed. After integration of CRRT into the NMP circuit, the composition of the perfusate was corrected to physiological values within 12 h, and this homeostasis was maintained during NMP for up to 7 d. Glucose and lactate levels, as measured in the CRRT ultrafiltrate, were strongly correlated with perfusate levels (r = 0.997, P < 0.001 and r = 0.999, P < 0.001, respectively). The integration of CRRT into the NMP system corrected the composition of the perfusate to near-physiological values, which could be maintained for up to 7 d. The ultrafiltrate can serve as an alternative to the perfusate to monitor concentrations of small molecules without potentially compromising sterility.

Large eye–head gaze shifts measured with a wearable eye tracker and an industrial camera

We built a novel setup to record large gaze shifts (up to 140∘\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$^\\circ $$\\end{document}). The setup consists of a wearable eye tracker and a high-speed camera with fiducial marker technology to track the head. We tested our setup by replicating findings from the classic eye–head gaze shift literature. We conclude that our new inexpensive setup is good enough to investigate the dynamics of large eye–head gaze shifts. This novel setup could be used for future research on large eye–head gaze shifts, but also for research on gaze during e.g., human interaction. We further discuss reference frames and terminology in head-free eye tracking. Despite a transition from head-fixed eye tracking to head-free gaze tracking, researchers still use head-fixed eye movement terminology when discussing world-fixed gaze phenomena. We propose to use more specific terminology for world-fixed phenomena, including gaze fixation, gaze pursuit, and gaze saccade.

Ultrasound reforms droplets.

Size-controlled monodisperse droplets are indispensable in food, cosmetics, and healthcare industries. Although emulsion formation from bulk phases is well-explored, a robust in situ method to continuously reform existing emulsions is unavailable. Remarkably, we introduce a continuous flow acousto-microfluidics technique which enables simultaneous trapping-coalescence-splitting of droplets to reform an existing polydisperse emulsion into size-controlled droplets with improved monodispersity. In contrast to conventional approaches, our platform enables controlling droplet characteristics in situ by regulating acoustic power without altering hydrodynamical parameters thereby improving response time and facilitates continuous nozzle-less clogging-free droplet generation from a liquid plug in a chamber instead of from a liquid stream at a narrow junction. The technique can process polydisperse droplets produced not only due to fluid-source fluctuations or unstable jetting regime but also externally by non-microfluidic or inexpensive setups. Our theoretical scaling suggests that the sum of capillary (Ca) and acousto-capillary (Caa) numbers ∼ (1), and predicts the generated droplet size, both agreeing well with the experimental findings. We identify acousto-visco-capillary number, Caav = (Ca Caa)1/2, which governs the generated droplet size. We also explore and characterize acoustic streaming- and coalescence-based mixing of samples inside the trapped plug. Distinctively, our platform is amenable to continuous mixing of inhomogeneous droplets, offering monodisperse mixed-sample droplets, and holds the potential to match current throughput standards through suitable design modifications.

LuBiA (Luciferase-Based Binding Assay): Glowing Peptides as Sensitive Probes to Study Ligand-Receptor Interactions.

The quantitative and qualitative biochemical description of molecular interactions is fundamental to the study of ligand/receptor pairs and their structure/function relationships. Bioactive peptides often are active at (sub-)nanomolar concentrations, indicating they have a high affinity for their sites of action, notably binding sites on receptors. Since such receptor proteins are commonly of low abundance, highly sensitive detection methods are required to study these ligand/receptor interactions. We present a protocol for an inexpensive luminescence-based detection setup in which the peptide ligand of interest is extended with the 11-amino acid HiBiTtag. This tag can be quantified easily down to fmol amounts by its ability to reconstitute the enzymatic activity of LgBiT, a truncated version of the Oplophorus gracilirostris luciferase.

The wing interference patterns (WIPs) of Parapanteles (Braconidae, Microgastrinae): demonstrating a powerful and accessible tool for species-level identification of small and clear winged insects

Wing interference patterns (WIPs) are color patterns of insect wings caused by thin film interference. Thin film interference is the same phenomenon responsible for the refracted spectral colors sometimes visible on soap bubbles. Insect WIPs are static patterns due to the variable thickness of wing membranes and the colors produced depend on the thicknesses of wing membranes. While WIPs have been studied in several taxa of small insects, they have not been broadly adopted by insect taxonomists. We surveyed WIPs in one moderate-sized genus of parasitoid wasps, Parapanteles (Braconidae: Microgastrinae). Using an inexpensive microscope camera set-up and free imaging and analysis software, we detected consistent WIP differences between Parapanteles species. In some cases, WIPs can be used to diagnose sibling species that would otherwise require SEM images to differentiate or DNA barcodes. Wing interference patters are an underemployed character that may be similarly useful in many other taxa of small clear-winged insects.

Balloon inflation revisited

In the present work, an inexpensive setup of an experiment of balloon inflation is described in order to measure its pressure and diameter. As already children know from blowing into such a (toy) balloon, the initially necessary high pressure level decreases with increasing balloon diameter. The original intention of this project has thus been to quantify this effect, which from a theoretical point of view is known as material instability. With a corresponding instrumentation of the test setup, it is additionally possible to realise and analyse further results for different load scenarios such as loading and unloading cycles, which are able to (re)produce by the presented system quite as easy.

Recent Advances in the Development of Near-Infrared Fluorescent Probes for the in Vivo Brain Imaging of Amyloid-β Species in Alzheimer's Disease.

The deposition of β-amyloid (Aβ) plaques in the parenchymal and cortical regions of the brain of Alzheimer's disease (AD) patients is considered the foremost pathological hallmark of the disease. The early diagnosis of AD is paramount in order to effective management and treatment of the disease. Developing near-infrared fluorescence (NIRF) probes targeting Aβ species is a potential and attractive approach suitable for the early and timely diagnosis of AD. The advantages of the NIRF probes over other tools include real-time detection, higher sensitivity, resolution, comparatively inexpensive experimental setup, and noninvasive nature. Currently, enormous progress is being observed in the development of NIRF probes for the in vivo imaging of Aβ species. Several strategies, i.e., the classical push-pull approach, "turn-on" effect, aggregation-induced emission (AIE), and resonance energy transfer (RET), have been exploited for development. We have outlined and discussed the recently emerged NIRF probes with different design strategies targeting Aβ species for ex vivo and in vivo imaging. We believe that understanding the recent development enables the prospect of the rational design of probes and will pave the way for developing future novel probes for early diagnosis of AD.

High resolution 2D plastic scintillator detectors for radiotherapy departments

Abstract Introduction Plastic scintillation detectors (PSD) have been developed for over four decades and are widely used in a variety of fields, but one can find relatively few reports of their clinical use compared to other dosimetric solutions. Material and methods The inexpensive detector setup made of a Saint-Gobain BC-400 plastic scintillator and commercially available on the market CMOS-based DSLR Pentax camera was investigated. Build PSD detectors were irradiated with 6, 10 and 15 MV flattening filtered (FF) and 6 and 10 MV flattening filter free (FFF) photon beams using a clinical linear accelerator. Data were processed using Matlab software to remove background and artefacts. A comparison of the spatial resolution parameters to the Gafchromic EBT3 films was performed. Results Average dose difference between TPS and PSD was 1.1%. The measured spatial resolution was 0.29 mm, and it differed from the film by 1.1%. MTF50 for PSD was 0.57 mm higher than the Gafchromic film. Signal to dose fit function with an R-square equal to 0.999 was established. The standard deviation of mean pixels value for a series of measurements was below 0.1%, for variable dose rate dependence was below 0.6% and for different energies 1.1%. It was demonstrated that such a setup allows a satisfactory signal-to-dose dependence and provides high spatial resolution at an affordable price compared to a 2D ion chamber or a diode detector array. Moreover, PSDs are reusable and provide a simple readout compared to Gafchromic films commonly used in radiotherapy departments. Conclusions Variable parameters of the camera allow to select signal values at the optimal level. The system presented excellent signal stability, high image resolution and a simple signal-to-dose relationship which encourages further work to investigate PSDs for use in radiation therapy departments.

Quantification of plastic shrinkage and plastic shrinkage cracking of the 3D printable concretes using 2D digital image correlation

There is great potential for 3D concrete printing to considerably transform the construction industry. An important step toward the transfer of this new technology into practical application is to gain the ability to control and mitigate plastic shrinkage of printed elements as well as related cracking. These phenomena occur at a very early age after the deposition of concrete due to the absence of formwork in the first place. The cracks can negatively influence durability, serviceability, and aesthetics of the printed structures. In this context, it is an important task to quantify plastic shrinkage of printed concrete and to capture the formation and propagation of cracks. The research at hand presents an easy-to-use, inexpensive experimental setup for quantifying the magnitude of plastic shrinkage and an accurate method for assessing plastic shrinkage cracking by means of 2D digital image correlation.