Abstract Fejér’s integral inequality is a weighted version of the Hermite-Hadamard inequality that holds for the class of convex functions. To derive his inequality, Fejér [Über die Fourierreihen, II, Math. Naturwiss, Anz. Ungar. Akad. Wiss. 24 (1906), 369–390] assumed that the weight function is symmetric w.r.t. the midpoint of the interval. In this study, without assuming any symmetry condition on the weight function, Fejér’s inequality is extended to the class of sub-biharmonic functions, namely, the set of functions f ∈ C 4 ( I ) f\in {C}^{4}\left({\mathbb{I}}) satisfying f ″ ″ ≤ 0 {f}^{^{\prime\prime} ^{\prime\prime} }\le 0 , where I {\mathbb{I}} is an interval of R {\mathbb{R}} . In the special case when the weight function is symmetric w.r.t. the midpoint of some interval, and the function f f is convex and sub-biharmonic, an interesting refinement of Fejér’s inequality is deduced. Moreover, this inequality is extended to a new class of functions defined on the plane, that we call the set of sub-biharmonic functions on the coordinates. Assuming in addition that the function is convex on the coordinates, a refinement of an inequality due to Dragomir [On the Hadamard’s inequality for convex functions on the co-ordinates in a rectangle from the plane, Taiwan. J. Math. 5 (2001), 775–788] is obtained.