Individual Sweeps Research Articles

Advancing the precision of thermal Hall measurements for novel materials research

Precision thermal Hall measurements unlock potential for unraveling fundamental thermal transport principles in novel materials. This work addresses the challenge of resolving sub-mK temperature differences in magnetic fields by employing high-precision capacitance thermometry with thermalized electronics, achieving a background noise of approximately ▪ at ▪. The novelty lies in the improvement and combination of innovative approaches, including the application of attenuators for thermal anchoring, a modular multi-stage approach to reduce thermal gradients, direct thermometer-sample assembly, and the use of individual capacitance bridges for faster measurements. The setup performance is demonstrated for the pyrochlore oxide terbium titanate and the longitudinal and transverse thermal conductivities agree with the literature. We show that the transverse temperature difference is proportional to the product of magnetic field times heating power for fields up to ▪, enabling improved statistics compared to individual sweeps analysis. We conduct an analysis of uncertainties in the transverse thermal conductivity, considering various thermometer calibrations for extracting temperature differences. This analysis reveals a significant influence of the longitudinal conductivity on the transverse thermal conductivity. These advancements allow for unprecedented temperature resolution and stability, while identified resolution limitations pave the way for next-generation setups.

A detailed analysis of single-channel Nav 1.5 recordings does not reveal any cooperative gating.

Cardiac voltage-gated sodium (Na+ ) channels (Nav 1.5) are crucial for myocardial electrical excitation. Recent studies based on single-channel recordings have suggested that Na+ channels interact functionally and exhibit coupled gating. However, the analysis of such recordings frequently relies on manual interventions, which can lead to bias. Here, we developed an automated pipeline to de-trend and idealize single-channel currents, and assessed possible functional interactions in cell-attached patch clamp experiments in HEK293 cells expressing human Nav 1.5 channels as well as in adult mouse and rabbit ventricular cardiomyocytes. Our pipeline involved de-trending individual sweeps by linear optimization using a library of predefined functions, followed by digital filtering and baseline offset. Subsequently, the processed sweeps were idealized based on the idea that the ensemble average of the idealized current identified by thresholds between current levels reconstructs at best the ensemble average current from the de-trended sweeps. This reconstruction was achieved by non-linear optimization. To ascertain functional interactions, we examined the distribution of the numbers of open channels at every time point during the activation protocol and compared it to the distribution expected for independent channels. We also examined whether the channels tended to synchronize their openings and closings. However, we did not uncover any solid evidence of such interactions in our recordings. Rather, our results indicate that wild-type Nav 1.5 channels are independent entities or exhibit only very weak functional interactions that are probably irrelevant under physiological conditions. Nevertheless, our unbiased analysis will be important for further studies examining whether auxiliary proteins potentiate functional Na+ channel interactions. KEY POINTS: Nav 1.5 channels are critical for cardiac excitation. They are part of macromolecular interacting complexes, and it was previously suggested that two neighbouring channels may functionally interact and exhibit coupled gating. Manual interventions when processing single-channel recordings can lead to bias and inaccurate data interpretation. We developed an automated pipeline to de-trend and idealize single-channel currents and assessed possible functional interactions between Nav 1.5 channels in HEK293 cells and cardiomyocytes during activation protocols using the cell-attached patch clamp technique. In recordings consisting of up to 1000 sweeps from the same patch, our analysis did not reveal any evidence of functional interactions or coupled gating between wild-type Nav 1.5 channels. Our unbiased analysis may be useful in further studies examining how Na+ channel interactions are affected by mutations and auxiliary proteins.

7‐3: A New Line‐by‐Line SWEEP Signal Generation Method for PWM Driving MicroLED TFT Pixel Circuit

This paper proposes a novel method to generate a SWEEP signal line‐by‐line for PWM driving microLED pixel circuit. The proposed method generates a SWEEP signal based on the linearly increasing voltage of the capacitor by flowing a constant current to the horizontal line parasitic capacitive load. The constant current is supplied by the sweep generation (SG) circuit in each line. Since the SG circuit utilizes the existing SCAN signals for the pixel circuit, additional SCAN or clock signals are not required. By using the proposed method, an individual SWEEP signal for each line can be generated without a significant increase in operational complexity in the display panel.

Turbulent Momentum Flux Behavior above a Fire Front in an Open-Canopied Forest

Atmospheric turbulent circulations in the vicinity of wildland fire fronts play an important role in the transfer of momentum into and out of combustion zones, which in turn can potentially affect the behavior and spread of wildland fires. The vertical turbulent transfer of momentum is accomplished via individual sweep, ejection, outward interaction, and inward interaction events, collectively known as sweep-ejection dynamics. This study examined the sweep-ejection dynamics that occurred before, during, and after the passage of a surface fire front during a prescribed fire experiment conducted in an open-canopied forest in the New Jersey Pine Barrens. High-frequency (10 Hz), tower-based, sonic anemometer measurements of horizontal and vertical wind velocity components in the vicinity of the fire front were used to assess the relative frequencies of occurrence of the different types of momentum-flux events, their contributions to the overall momentum fluxes, and their periodicity patterns. The observational results suggest that the presence of surface fire fronts in open-canopied forests can substantially change the sweep-ejection dynamics that typically occur when fires are not present. In particular, sweep events resulting in the downward transport of high horizontal momentum air from above were found to be more prominent during fire-front-passage periods.

Contrast-enhanced ultrasound for screening hepatocellular carcinoma: an implemented program at a semi-rural academic center.

PurposeTo describe our early experience using a contrast-enhanced ultrasound (CEUS) protocol for surveillance of hepatocellular carcinoma (HCC) at a semi-rural academic medical center.MethodsRetrospective, longitudinal study of the first 100 patients who underwent CEUS liver screening imaging over 2 years. Each patient underwent a standard of care abdominal ultrasound, which was checked with the radiologist, who searched for a focal lesion on the cine clips to target specifically with contrast. If none was present, the HCC contrast-enhanced screening protocol consisting of individual sweeps of the right and left lobes was performed from 0 to 60 s and 3–4 min post contrast—Lumason was utilized. Images, report details, and patient demographics were analyzed.Results66 men and 34 women (average age, 59 ± 13 years) were included. On average, the distance from patient’s home to our institution was 39 miles (range 2–179 miles). The majority of our patients were covered under Private insurance (46%) with 43% covered by Medicare. CEUS exams on average took 35 min to complete. Lumason was administered in split doses for an average total of 5 mL per exam. Of the 10 lesions identified, there were five LI-RADS 3, two LI-RADS 4, one LI-RADS 5, two LI-RADS M, and one bland portal vein thrombus. There were no complications reported.ConclusionThis semi-rural single-center study demonstrates the feasibility of starting a HCC CEUS screening program. CEUS can be performed in conjunction with routine ultrasound imaging with added benefit of identifying and characterizing lesions in one setting.

Effects of Transient Cerebral Hypoperfusion on Human Auditory Brainstem Responses

Adequate cerebral blood flow perfusion is crucial to maintaining brain function. The auditory brainstem response (ABR) has been demonstrated to be the most powerful diagnostic tool for assessing auditory function. This study aimed to determine the ABR under transient cerebral hypoperfusion caused by using oscillatory lower body negative pressure (OLBNP). As chronic cerebral hypoperfusion has been associated with the prolongation of the latency of ABR waves, we hypothesized that the fluctuation of cerebral blood flow perfusion also affects this latency. As prior data showing that cerebral autoregulation is not effectively engaged when pressure fluctuations are shorter than 30 sec periods (i.e., higher than 0.03 Hz), we used the sinusoidal pattern of an 18 sec period (0.056 Hz) of 0 ~ - 40 mmHg OLBNP to induce transient cerebral hypoperfusion. Here, we measured ABR and cerebral blood flow velocity (MCAv) during 12 min of control and 12 min of OLBNP (40 cycle of 18-sec periods) in 15 subjects (3 of whom were excluded from the analysis due to an insufficient number of data for ABR). Periodical changes in cerebrovascular variables were aligned relative to each 18 sec of OLBNP cycle onset and were subdivided into three phases (phase-1: 0 ~ 6 sec, phase-2: 6 ~ 12 sec, and phase-3: 12 ~ 18 sec of OLBNP onset). For the ABR analysis, a total of 6800 individual sweeps were recorded for 12 min in the control and OLBNP conditions, and the recorded sweeps in the OLBNP condition were subdivided into three phases. We observed that OLBNP caused cyclic fluctuations of MCAv. One-way repeated-measures analysis of variance (ANOVA) and the post-hoc comparison revealed that MCAv decreased significantly in phases-1 and -2 compared to the control and phase-3 (F(3, 33) = 16.54, p < 0.001). As for the ABR data, ANOVA and the post-hoc comparison revealed that the latency of wave V of ABR at phase-2 was delayed compared to phase-1 (F(3, 33) = 3.70, p < 0.05). Moreover, the results showed that the inter-individual difference in the variance over the phase conditions of the latency of wave V was significantly related to that of cerebral blood flow (r = 0.641, p < 0.05). Our data demonstrate that the fluctuation of cerebral blood flow perfusion could lead to the prolongation of the latency of ABR waves. This suggests that the transient cerebral hypoperfusion induced by an acute orthostatic blood shift to the lower body could impair auditory function.

Trace metal and isotopic depth profiles through the Abitibi cratonic mantle

Geophysical imaging of trans-lithospheric structures provide a spatial link between ore deposits in the crust and the underlying cratonic mantle. However, the deep lithosphere's role in ore deposit genesis remains poorly understood because remotely acquired datasets do not provide any direct constraints on the behaviour of ore elements within these mantle-roots. The abundance and behaviour of ore elements governs the metallic endowment of the cratonic mantle and the economic potential of mantle-derived magmas. Herein we present in situ electron probe microanalysis (EPMA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) geochemical datasets for clinopyroxene and olivine mantle xenocrysts from the Jurassic Kirkland Lake kimberlite field, Abitibi greenstone belt, Canada. We specifically focus on unconventional trace elements, including ore elements with chalcophile and/or siderophile affinities (Ag-As-Au-Bi-Cu-Mo-Pb-Pt-Pd-Sb-Se-Sn-Te-W-Zn). Robust principal component analysis suggests that low-T, large-ion lithophile element alteration (Ba-Sr), which likely occurred during kimberlite emplacement, represents the largest source of variance for the xenocryst dataset. PT-dependent element partitioning during sub-solidus equilibration represents the second most important control on olivine and clinopyroxene chemistry. We demonstrate that least-altered, high-PT mantle silicates are, in fact, a significant mineral host for a range of ore elements (Cu-Zn ± Ag ± As ± Se ± Sn ± Mo) within equilibrated, garnet peridotite at depth (70–190 km). Statistical analysis of the raw, individual mass sweeps for each LA-ICP-MS signal suggest that the most abundant ore elements (Cu-Zn) occur predominantly as PT-dependent substitution reactions with the dominant mineral-forming elements, rather than as inclusions. A subset of high-PT olivine (160–180 km) yields Fe-Ni-S-poor and Na (Au ± Pt ± Pd)-rich compositions, which may reflect metasomatism, sulphide segregation and trapping of precious metal-bearing fluids at the base of the lithosphere. These anomalous mantle fragments possibly represent the first, direct sampling of precious metal-modified mantle peridotite beneath the Abitibi. Mid-PT olivine xenocrysts (70–120 km), which yield Mg-rich and high field-strength element-poor compositions, document a highly melt-depleted segment of mantle peridotite coincident with and below a shallow-dipping, low-seismic-velocity anomaly and conductive feature of the Kirkland Lake mid-lithosphere at 70–100 km. We speculate that the trace element signature of mid-PT xenocrysts documents the re-distribution of high-charge and incompatible elements from refractory garnet peridotite to phlogopite- and/or amphibole-bearing peridotite with conductive metasomatic up-flow zones. The rapid, sub-solidus diffusion of elements at high-T suggest that these processes likely occurred during, and/or immediately preceding, kimberlite volcanism. New in situ Pb isotope analyses of clinopyroxene xenocrysts sampled from metasomatized, low-Al garnet peridotite, however, also document ancient metasomatic events that likely pre-date Jurassic kimberlitic volcanism by at least one billion years.

System identification of flexible aircraft in frequency domain

PurposeThe purpose of this paper is to identify the flexible aircraft model accurately from the frequency responses.Design/methodology/approachThe frequency domain output error method is used to estimate the aerodynamic (rigid body and elastic body) derivatives, and mode shape parameters in the process of identification of flexible aircraft model. The accurate identification of lightly damped low frequency rigid-body response modes requires a careful selection of the frequency sweep length and the fast Fourier transform (FFT) window size, as the FFT window length cannot be longer than any individual sweep records. To address this issue, an effort is made to derive the FFT window length for the application of frequency domain estimation approach.FindingsThe investigations are initially made to select a suitable FFT window size for the accurate identification of the lightly damped low frequency rigid-body response modes of the flexible aircraft. Subsequently, frequency domain estimation approach is applied to simulated data of flexible aircraft. Besides the stability and control derivatives, the structural modes of the flexible aircraft are also estimated as part of state space model identification, and it is shown that all the model parameter estimates are accurate. Identification of such flexible aircraft aerodynamic (rigid body and elastic body) derivatives and structural mode shape parameters will lead to mathematical models of flexible aircraft that are accurate over a wide frequency range. The identified models are validated using the time response of frequency sweep data.Research limitations/implicationsAircraft system identification is an integral part of aerospace system design and life cycle process. This becomes a complex process when the aircraft has significant effects of flexibility on the flight dynamics, especially as the frequencies of the elastic modes become lower and approach those of the rigid body modes. Thus, an integrated mathematical model of flexible aircraft is required to develop, and it should be valid for a wide frequency range and relevant for the design of flight control system.Originality/valueThis paper focuses on the application of frequency domain approach to identify the valid model of flexible aircraft by estimating the aerodynamic (rigid body and elastic body) derivatives and structural mode shape parameters of flexible aircraft. The unknown frequencies of structural modes are also able to identify accurately in frequency domain. This gives more value addition to analyze the flight data of flexible aircraft, as it is challenging problem in parameter estimation of flexible aircraft.

SCM Adaptation to Improve Scanning Rate in RF Interferometry Applications

In this letter, we present a novel structure for performing subcarrier multiplexing (SCM) to improve the scanning rate in low coherence interferometry (LCI) systems combined with the microwave photonics (MWP) technology. In this MWP-LCI proposal, the optical path differences (OPDs) produced by different samples between the arms of an interferometer are closely related to the central frequency of different RF resonances generated in the RF domain. By the proposed adaptation of the SCM technique, “M” subcarriers are multiplexed in the modulation stage and each subcarrier is set to sweep simultaneously a concrete part of the spectrum. The complete electrical transfer function of the structure is obtained combining each individual sweep. Therefore, a considerable reduction of the sweep time is provided to collect the complete electrical transfer function. Therefore, the scanning rate is reduced according to the number of subcarriers (M) employed in the multiplexing stage. An OPD range of 8 mm is achieved with a constant resolution of $120~\mu \text{m}$ in the whole range. Finally, a maximum sensitivity of 60 dB is also reached for that operation range.

Effects of Charge Preheating Methods on the Combustion Phasing Limitations of an HCCI Engine With Negative Valve Overlap

Homogeneous charge compression ignition (HCCI) has the potential to reduce both fuel consumption and NOx emissions compared to normal spark-ignited (SI) combustion. For a relatively low compression ratio engine, high unburned temperatures are needed to initiate HCCI combustion, which is achieved with large amounts of internal residual or by heating the intake charge. The amount of residual in the combustion chamber is controlled by a recompression valve strategy, which relies on negative valve overlap (NVO) to trap residual gases in the cylinder. A single-cylinder research engine with fully-flexible valve actuation is used to explore the limits of HCCI combustion phasing at a load of ∼3 bar gross indicated mean effective pressure (IMEPg). This is done by performing two individual sweeps of (a) internal residual fraction (via NVO) and (b) intake air temperature to control combustion phasing. It is found that increasing both of these variables advances the phasing of HCCI combustion, which leads to increased NOx emissions and a higher ringing intensity. On the other hand, a reduction in these variables leads to greater emissions of CO and HC, as well as a decrease in combustion stability. A direct comparison of the two sweeps suggests that the points with elevated intake temperatures are more prone to ringing as combustion is advanced and less prone to instability and misfire as combustion is retarded. This behavior can be explained by compositional differences (air versus residual gas dilution) which lead to variations in burn rate and peak temperature. As a final study, two additional NVO sweeps are performed while holding intake temperature constant at 30 °C and 90 °C. Again, it is seen that for higher intake temperatures, combustion is more susceptible to ringing at advanced timings and more resistant to instability/misfire at retarded timings.

Rapid dopaminergic and GABAergic modulation of calcium and voltage transients in dendrites of prefrontal cortex pyramidal neurons

The physiological responses of dendrites to dopaminergic inputs are poorly understood and controversial. We applied dopamine on one dendritic branch while simultaneously monitoring action potentials (APs) from multiple dendrites using either calcium-sensitive dye, voltage-sensitive dye or both. Dopaminergic suppression of dendritic calcium transients was rapid (<0.5 s) and restricted to the site of dopamine application. Voltage waveforms of backpropagating APs were minimally altered in the same dendrites where dopamine was confirmed to cause large suppression of calcium signals, as determined by dual voltage and calcium imaging. The dopamine effects on dendritic calcium transients were fully mimicked by D1 agonists, partially reduced by D1 antagonist and completely insensitive to protein kinase blockade; consistent with a membrane delimited mechanism. This dopamine effect was unaltered in the presence of L-, R- and T-type calcium channel blockers. The somatic excitability (i.e. AP firing) was not affected by strong dopaminergic stimulation of dendrites. Dopamine and GABA were then sequentially applied on the same dendrite. In contrast to dopamine, the pulses of GABA prohibited AP backpropagation distally from the application site, even in neurons with natural Cl− concentration (patch pipette removed). Thus, the neocortex employs at least two distinct mechanisms (dopamine and GABA) for rapid modulation of dendritic calcium influx. The spatio-temporal pattern of dendritic calcium suppression described in this paper is expected to occur during phasic dopaminergic signalling, when midbrain dopaminergic neurons generate a transient (0.5 s) burst of APs in response to a salient event.

PICK1-mediated Glutamate Receptor Subunit 2 (GluR2) Trafficking Contributes to Cell Death in Oxygen/Glucose-deprived Hippocampal Neurons

Oxygen and glucose deprivation (OGD) induces delayed cell death in hippocampal CA1 neurons via Ca2+/Zn2+-permeable, GluR2-lacking AMPA receptors (AMPARs). Following OGD, synaptic AMPAR currents in hippocampal neurons show marked inward rectification and increased sensitivity to channel blockers selective for GluR2-lacking AMPARs. This occurs via two mechanisms: a delayed down-regulation of GluR2 mRNA expression and a rapid internalization of GluR2-containing AMPARs during the OGD insult, which are replaced by GluR2-lacking receptors. The mechanisms that underlie this rapid change in subunit composition are unknown. Here, we demonstrate that this trafficking event shares features in common with events that mediate long term depression and long term potentiation and is initiated by the activation of N-methyl-d-aspartic acid receptors. Using biochemical and electrophysiological approaches, we show that peptides that interfere with PICK1 PDZ domain interactions block the OGD-induced switch in subunit composition, implicating PICK1 in restricting GluR2 from synapses during OGD. Furthermore, we show that GluR2-lacking AMPARs that arise at synapses during OGD as a result of PICK1 PDZ interactions are involved in OGD-induced delayed cell death. This work demonstrates that PICK1 plays a crucial role in the response to OGD that results in altered synaptic transmission and neuronal death and has implications for our understanding of the molecular mechanisms that underlie cell death during stroke.

Short-Term Dynamics of Synaptic Transmission Within the Excitatory Neuronal Network of Rat Layer 4 Barrel Cortex

The short-term plasticity of synaptic transmission between excitatory neurons within a barrel of layer 4 rat somatosensory neocortex was investigated. Action potentials in presynaptic neurons at frequencies ranging from 1 to 100 Hz evoked depressing postsynaptic excitatory postsynaptic potentials (EPSPs). Recovery from synaptic depression followed an exponential time course with best-fit parameters that differed greatly between individual synaptic connections. The average maximal short-term depression was close to 0.5 with a recovery time constant of around 500 ms. Analysis of each individual sweep showed that there was a correlation between the amplitude of the response to the first and second action potentials such that large first EPSPs were followed by smaller than average second EPSPs and vice versa. Short-term depression between excitatory layer 4 neurons can thus be termed use dependent. A simple model describing use-dependent short-term plasticity was able to closely simulate the experimentally observed dynamic behavior of these synapses for regular spike trains. More complex irregular trains of 10 action potentials occurring within 500 ms were initially well described, but during the train errors increased. Thus for short periods of time the dynamic behavior of these synapses can be predicted accurately. In conjunction with data describing the connectivity, this forms a first step toward computational modeling of the excitatory neuronal network of layer 4 barrel cortex. Simulation of whisking-evoked activity suggests that short-term depression may provide a mechanism for enhancing the detection of objects within the whisker space.

Suppression of EMG activity by transcranial magnetic stimulation in human subjects during walking.

1. The involvement of the motor cortex during human walking was evaluated using transcranial magnetic stimulation (TMS) of the motor cortex at a variety of intensities. Recordings of EMG activity in tibialis anterior (TA) and soleus muscles during walking were rectified and averaged. 2. TMS of low intensity (below threshold for a motor-evoked potential, MEP) produced a suppression of ongoing EMG activity during walking. The average latency for this suppression was 40.0 +/- 1.0 ms. At slightly higher intensities of stimulation there was a facilitation of the EMG activity with an average latency of 29.5 +/- 1.0 ms. As the intensity of the stimulation was increased the facilitation increased in size and eventually a MEP was clear in individual sweeps. 3. In three subjects TMS was replaced by electrical stimulation over the motor cortex. Just below MEP threshold there was a clear facilitation at short latency (approximately 28 ms). As the intensity of the electrical stimulation was reduced the size of the facilitation decreased until it eventually disappeared. We did not observe a suppression of the EMG activity similar to that produced by TMS in any of the subjects. 4. The present study demonstrates that motoneuronal activity during walking can be suppressed by activation of intracortical inhibitory circuits. This illustrates for the first time that activity in the motor cortex is directly involved in the control of the muscles during human walking.

Cortical potentials at the frequency of absolute wrist velocity become phase-locked during slow sinusoidal tracking movements.

A 1-Hz rhythmic event-related potential was recorded at the scalp during performance of a 0.5-Hz tracking task. At cortical motor areas, negative peaks occurred 10-20 ms after peak tracking speeds. Analysis of single sweeps suggested that EEG phase was reset at initiation of the tracking motion and then maintained a constant relationship to wrist speed until task completion. Frequency analysis indicated that rhythm appearance in the averaged potential was predominantly due to phase-locking, because there was no tracking-related increase in 1 Hz amplitude within individual sweeps. While tracking, phase-locking was present over bilateral parieto-occipital and frontal regions, with a slight predominance at the contralateral frontal region. When subjects observed the target motion, phase-locking was localized to parieto-occipital regions. We suggest mental processes such as visual processing, visuomotor coordination and real-time motor planning are reflected in the pacing of localized cortical potential fluctuations.

Gating of single N-type calcium channels recorded from bullfrog sympathetic neurons.

For many neurons, N-type calcium channels provide the primary pathway for calcium influx during an action potential. We investigated the gating properties of single N-type calcium channels using the cell-attached patch technique. With 100 mM Ba2+ in the pipet, mean N-channel open probability (Po, measured over 100 ms) increased with depolarization, but the range at a single voltage was large (e.g., Po at +40 mV ranged from 0.1 to 0.8). The open dwell time histograms were generally well fit by a single exponential with mean open time (tauo) increasing from 0.7 ms at +10 mV to 3.1 ms at +40 mV. Shut time histograms were well fit by two exponentials. The brief shut time component (taush1 = 0.3 ms) did not vary with the test potential, while the longer shut time component (taush2) decreased with voltage from 18.9 ms at +10 mV to 2.3 ms at +40 mV. Although N-channel Po during individual sweeps at +40 mV was often high ( approximately 0.8), mean Po was reduced by null sweeps, low Po gating, inactivation, and slow activation. The variability in mean Po across patches resulted from differences in the frequency these different gating processes were expressed by the channels. Runs analysis showed that null sweeps tended to be clustered in most patches, but that inactivating and slowly activating sweeps were generally distributed randomly. Low Po gating (Po = 0.2, tauo = 1 ms at +40 mV) could be sustained for approximately 1 min in some patches. The clustering of null sweeps and sweeps with low Po gating is consistent with the idea that they result from different modes of N-channel gating. While Po of the main N-channel gating state is high, the net Po is reduced to a maximum value of close to 0.5 by other gating processes.

Sorted averaging--principle and application to auditory brainstem responses.

A new averaging method for evoked potentials, called sorted averaging, is presented. The method requires an ensemble of sweeps stored in memory and is based on the principle of interchangeability of individual sweeps within this ensemble. Sorted averaging is applied by sorting all sweeps according to their estimated background noise and successive averaging of this sorted ensemble, starting with low-noise sweeps. Signal-to-noise power ratio (SNR2) is estimated by calculating the inverse single point variance (Elberling & Don, 1984). This SNR2 estimate increases linearly with the number of sweeps for standard averaging and shows a clear maximum for sorted averaging. Auditory brainstem responses to click stimuli at 70 dB nHL in 20 normal hearing subjects were recorded and 4000 individual sweeps during each run were stored for analysis. In an off-line analysis, SNR2 for standard averaging with 10 microV artefact rejection, for weighted averaging and for sorted averaging were calculated. Sorted averaging was found to yield a significantly higher SNR2.

Removing non-random artifacts from patch clamp traces

Analysis and interpretation of current records from cellular membranes or lipid bilayers is complicated by perturbations. These perturbations can originate from random noise or deterministic artifacts as capacitive currents caused by step depolarization's, drift, 50 Hz pick up or microphonics. Accurate removal of deterministic perturbations is a prerequisite for further analysis of ion channel kinetics. In this report we present two methods developed for parameter estimation of such artifacts in order to remove these perturbations from single channel patch clamp traces. For both methods artifact removal does not require sweeps lacking channel activity. The first method was designed to extract artifacts from sweeps showing moderate channel activity. Within a certain number of sweeps the artifacts should remain rather constant. The second method allows for artifact removal from one individual sweep with channel activity. With the second method, correction for drift, pick up and microphonics is possible without long periods of minor channel activity. To evaluate the correctness of artifact removal a specific test was set up. The methods were carefully examined using simulated records for a wide range of parameters found in single channel experiments. Further, the developed algorithms were applied to original records obtained from cardiac and recombinant L-type Ca 2+ channels.

Comparison of methods for reducing residual noise in suprathreshold early auditory evoked potential registration

The level of residual noise in auditory brainstem responses (ABR) depends not only on the number of averages but also on the amplitude of background noise and on the frequency of artifacts. This paper describes the influence of digital filtering and of different methods of artifact suppression on the residual noise of ABRs. Amplitude of background noise was estimated for 1033 ABRs recorded under suprathreshold stimulation (70 and 90 dB nHL) in 251 subjects. In 45 ABR recordings in 15 subjects, all 4000 individual sweeps were stored for off-line simulation. The power spectrum of background noise was investigated using an FFT analyzer. A great variability of mean noise amplitude was found both between subjects and in the recordings for each subject. Depending on the slope of the analogue 100-Hz high-pass filter, mean RMS values of background noise of 4.2 microV (6 dB/Oct.) and 2.5 microV (12 dB/Oct.), respectively, were found. Digital high pass filtering before averaging was found to increase the signal-to-noise ratio (SNR) considerably. Results indicate that (i) effective suppression of low-frequency noise components can only be achieved by zero phase digital filtering and (ii) if clipping of noise amplitude to 25 microV is used, optimized artifact rejection as weighted averaging or adopted artifact rejection levels have only small effect on the SNR.

A test of the hendrickson postulate that reduced EEG response variance causes increased aep contour length: Implications for the ‘neural transmission errors’ theory of intelligence

EEG responses to auditory tones were recorded from 76 human subjects in six different age groups with equal numbers of males and females in each group. Care was taken to reproduce the conditions described by D. E. Hendrickson (1982) who had earlier reported high correlations between intelligence test scores and her new ‘string’ and ‘variance’ measures of EEG response differences. The results obtained in the present study show that the Hendrickson string variable derives from EEG activity in the high frequency band whereas the Hendrickson variance variable derives from activity in the low frequency band. In addition, there is a high positive correlation between the string variable and the variance of corresponding high frequency activity in individual sweeps. These results disprove the Hendricksons' ‘neural transmission errors’ theory of intelligence which requires a negative correlation between the number and amplitude of peaks in the averaged evoked potential (AEP) waveform and the variance of corresponding activity in the individual EEG epochs from which the AEP is derived. It is concluded that the Hendrickson theory fails at the critical point where it seeks to link its hypothetical transmission errors to actual EEG differences that sometimes correlate with intelligence differences.