Individual Species Research Articles

Mercury in four common fishes sold in Algeria and associated humans risk.

This study aimed to analyse the concentrations of mercury in fish samples available in the Algerian market and evaluate the potential health risks associated with their consumption. A total of 135 fish samples, representing the species Sardina pilchardus, Merluccius merluccius, Sparus aurata and Auxis rochie, were collected and analysed. Mercury levels were determined using thermal decomposition amalgamation Atomic Absorption Spectrophotometry. Health risks were evaluated by calculating hazard quotients. The overall mean concentration was 0.19 ± 0.11 mg/kg across all species. Individual species concentrations were recorded as 0.17 ± 0.08 mg/kg for Sardina pilchardus, 0.26 ± 0.19 mg/kg for Merluccius merluccius, 0.27 ± 0.18 mg/kg for Sparus aurata and 0.23 ± 0.13 mg/kg for Auxis rochei. Hazard quotients were below 1, indicating low health risk for fish consumers. Nevertheless, it is recommended to conduct periodic monitoring of heavy metal levels in fish, coupled with ongoing risk assessments, to ensure continued consumer protection.

Investigation of heavy metals uptake in root-shoot of native plant species adjoining wastewater channels.

Metal pollution in water, soil, and vegetation is an emerging environmental issue. Therefore, this study investigated the abundance of heavy metals (HMs) within roots and shoots of native plant species i.e., Bromus pectinatus, Cynodon dactylon, Poa annua, Euphorbia heliscopa, Anagallis arvensis, and Stellaria media grown in the adjoining area of municipal wastewater channels of a Pakistani city of Abbottabad. HMs concentrations (mg L-1) in municipal wastewater were: chromium (Cr) (0.55) > nickel (Ni) (0.09) > lead (Pb) (0.07) > cadmium (Cd) (0.03). Accumulation of HMs in both roots and shoots of plant species varied as B. pectinatus > C. dactylon > P. annua > E. heliscopa > A. arvensis > S. media. Irrespective of the plant species, roots exhibited higher concentrations of HMs than shoots. Higher amount of Cr (131.70 mg kg-1) was detected in the roots of B. pectinatus and the lowest amount (81 mg kg-1) in A. arvensis, Highest Cd concentration was found in the shoot of B. pectinatus and the lowest in the E. heliscopa. The highest concentration of Ni was found in the roots of S. media (37.40 mg kg-1) and the shoot of C. dactylon (15.70 mg kg-1) whereas the lowest Ni concentration was achieved in the roots of A. arvensis (12.10 mg kg-1) and the shoot of E. heliscopa (5.90 mg kg-1). The concentration of HMs in individual plant species was less than 1000 mg kg-1. Considering the higher values (> 1) of biological concentration factor (BCF), biological accumulation co-efficient (BAC), and translocation factor (TF), B. pectinatus and S. media species showed greater potential for HMs accumulation than other species. Therefore, these plants might be helpful for the remediation of HM-contaminated soil.

Comparative transcriptomic analysis provides insights into the genetic networks regulating oil differential production in oil crops

BackgroundPlants differ more than threefold in seed oil contents (SOCs). Soybean (Glycine max), cotton (Gossypium hirsutum), rapeseed (Brassica napus), and sesame (Sesamum indicum) are four important oil crops with markedly different SOCs and fatty acid compositions.ResultsCompared to grain crops like maize and rice, expanded acyl-lipid metabolism genes and relatively higher expression levels of genes involved in seed oil synthesis (SOS) in the oil crops contributed to the oil accumulation in seeds. Here, we conducted comparative transcriptomics on oil crops with two different SOC materials. In common, DIHYDROLIPOAMIDE DEHYDROGENASE, STEAROYL-ACYL CARRIER PROTEIN DESATURASE, PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE, and oil-body protein genes were both differentially expressed between the high- and low-oil materials of each crop. By comparing functional components of SOS networks, we found that the strong correlations between genes in “glycolysis/gluconeogenesis” and “fatty acid synthesis” were conserved in both grain and oil crops, with PYRUVATE KINASE being the common factor affecting starch and lipid accumulation. Network alignment also found a conserved clique among oil crops affecting seed oil accumulation, which has been validated in Arabidopsis. Differently, secondary and protein metabolism affected oil synthesis to different degrees in different crops, and high SOC was due to less competition of the same precursors. The comparison of Arabidopsis mutants and wild type showed that CINNAMYL ALCOHOL DEHYDROGENASE 9, the conserved regulator we identified, was a factor resulting in different relative contents of lignins to oil in seeds. The interconnection of lipids and proteins was common but in different ways among crops, which partly led to differential oil production.ConclusionsThis study goes beyond the observations made in studies of individual species to provide new insights into which genes and networks may be fundamental to seed oil accumulation from a multispecies perspective.

Climate change could negate U.S. forest ecosystem service benefits gained through reductions in nitrogen and sulfur deposition

Climate change and atmospheric deposition of nitrogen (N) and sulfur (S) impact the health and productivity of forests. Here, we explored the potential impacts of these environmental stressors on ecosystem services provided by future forests in the contiguous U.S. We found that all stand-level services benefitted (+ 2.6 to 8.1%) from reductions in N+S deposition, largely attributable to positive responses to reduced S that offset the net negative effects of lower N levels. Sawtimber responded positively (+ 0.5 to 0.6%) to some climate change, but negatively (− 2.4 to − 3.8%) to the most extreme scenarios. Aboveground carbon (C) sequestration and forest diversity were negatively impacted by all modelled changes in climate. Notably, the most extreme climate scenario eliminated gains in all three services achieved through reduced deposition. As individual tree species responded differently to climate change and atmospheric deposition, associated services unique to each species increased or decreased under future scenarios. Our results suggest that climate change should be considered when evaluating the benefits of N and S air pollution policies on the services provided by U.S. forests.

Comparative Assessment of Plant Species and Their Taxa Distribution between the Capital Cities of Akwa Ibom and Bayelsa States, Nigeria

The study examined the comparative assessment of plant species and their taxa distribution between the Capital Cities of Akwa Ibom and Bayelsa States, Nigeria. The study established quadrats of 30mx200m along road (transects) in GRAs of Uyo City, Akwa Ibom State and Yenagoa City, Bayelsa State labelled as sampled sites and a quadrat of 100mx100m were established as control sites (secondary forest) at a minimum of 300m from the sampled sites. Descriptive statistics were employed to analyse the data. Findings revealed that that a total of 32 plant individual species types were found in the sampled sites in Uyo while a total of 16 plant individual species types belonging to 13 families were found in Yenagoa. Also, the 32 individual plant species found under the sampled sites belong to 20 families with Arecaceae and Moraceae having the highest species individuals in Uyo Town while 26 individual plant species were identified belonging to 19 families with Apocynaceae and Guthiferae producing the highest numbers of species individuals in Yenagoa. The study can be concluded that there was variation in the plant taxa between Akwa Ibom State and Yenagoa State as the plant composition in the Uyo is more than that of Yenagoa Town suggesting the influence of the level of urbanization and other anthropogenic activities. The study recommended that urban greening activities should commence immediately in the areas where there is a shortage of plant composition.

Assessing the effect of COVID 19 lockdowns on the composition of organic compounds and potential source of PM2.5 in Hanoi, Vietnam.

The ambient air quality during COVID-19 lockdowns has been improved in many cities in the world. This study is to assess the changes in persistent organic pollutants in PM2.5 during the COVID-19 lockdown in Hanoi. Individual organic species in PM2.5 ((e.g., polycyclic aromatic hydrocarbons (PAHs), polychlorobiphenyls (PCBs), and organochlorine pesticides (OCPs)) were measured in an urban residential area in Hanoi from before the March 10th to April 22nd, 2020, including before the partial lockdown (BL) and the partial lockdown (PL) phases. During the PL phase, the concentration of Σ14PAHs and Σ28PCBs was reduced by 38 and 52% compared with the BL period, respectively. The diagnostic ratio method implied that the sources of PAHs within the PL phase had a less effect on traffic and industrial activities than in the BL phase. The characteristic ratio method indicated that PCBs were mixed by commercial product and combustion process in both the BL and the PL periods, however, the source of PCBs in the BL phase was influenced by municipal waste incineration more than those in the PL phase. The decreasing concentration of Σ20OCPs during the partial lockdown was attributed to the restriction of human activities during the quarantine period. The results suggested that the source of OCPs was probably derived from the usage of pesticides in current and, historical degradation or the transportation of pesticides from the soil to the atmosphere.

Macroevolution of the plant-hummingbird pollination system.

Plant-hummingbird interactions are considered a classic example of coevolution, a process in which mutually dependent species influence each other's evolution. Plants depend on hummingbirds for pollination, whereas hummingbirds rely on nectar for food. As a step towards understanding coevolution, this review focuses on the macroevolutionary consequences of plant-hummingbird interactions, a relatively underexplored area in the current literature. We synthesize prior studies, illustrating the origins and dynamics of hummingbird pollination across different angiosperm clades previously pollinated by insects (mostly bees), bats, and passerine birds. In some cases, the crown age of hummingbirds pre-dates the plants they pollinate. In other cases, plant groups transitioned to hummingbird pollination early in the establishment of this bird group in the Americas, with the build-up of both diversities coinciding temporally, and hence suggesting co-diversification. Determining what triggers shifts to and away from hummingbird pollination remains a major open challenge. The impact of hummingbirds on plant diversification is complex, with many tropical plant lineages experiencing increased diversification after acquiring flowers that attract hummingbirds, and others experiencing no change or even a decrease in diversification rates. This mixed evidence suggests that other extrinsic or intrinsic factors, such as local climate and isolation, are important covariables driving the diversification of plants adapted to hummingbird pollination. To guide future studies, we discuss the mechanisms and contexts under which hummingbirds, as a clade and as individual species (e.g. traits, foraging behaviour, degree of specialization), could influence plant evolution. We conclude by commenting on how macroevolutionary signals of the mutualism could relate to coevolution, highlighting the unbalanced focus on the plant side of the interaction, and advocating for the use of species-level interaction data in macroevolutionary studies.

Combining mesocosms with models reveals effects of global warming and ocean acidification on a temperate marine ecosystem.

Ocean warming and species exploitation have already caused large-scale reorganization of biological communities across the world. Accurate projections of future biodiversity change require a comprehensive understanding of how entire communities respond to global change. We combined a time-dynamic integrated food web modeling approach (Ecosim) with previous data from community-level mesocosm experiments to determine the independent and combined effects of ocean warming, ocean acidification and fisheries exploitation on a well-managed temperate coastal ecosystem. The mesocosm parameters enabled important physiological and behavioral responses to climate stressors to be projected for trophic levels ranging from primary producers to top predators, including sharks. Through model simulations, we show that under sustainable rates of fisheries exploitation, near-future warming or ocean acidification in isolation could benefit species biomass at higher trophic levels (e.g., mammals, birds, and demersal finfish) in their current climate ranges, with the exception of small pelagic fishes. However, under warming and acidification combined, biomass increases at higher trophic levels will be lower or absent, while in the longer term reduced productivity of prey species is unlikely to support the increased biomass at the top of the food web. We also show that increases in exploitation will suppress any positive effects of human-driven climate change, causing individual species biomass to decrease at higher trophic levels. Nevertheless, total future potential biomass of some fisheries species in temperate areas might remain high, particularly under acidification, because unharvested opportunistic species will likely benefit from decreased competition and show an increase in biomass. Ecological indicators of species composition such as the Shannon diversity index decline under all climate change scenarios, suggesting a trade-off between biomass gain and functional diversity. By coupling parameters from multilevel mesocosm food web experiments with dynamic food web models, we were able to simulate the generative mechanisms that drive complex responses of temperate marine ecosystems to global change. This approach, which blends theory with experimental data, provides new prospects for forecasting climate-driven biodiversity change and its effects on ecosystem processes.

COMPOSITION, ABUNDANCE AND DISTRIBUTION OF NON-CICHLID FISH COMMUNITY IN OGBO STREAM AND ITS FLOODPLAIN, ENHWE, DELTA STATE, NIGERIA

A preliminary survey of the composition, abundance and distribution of non-cichlid fish community was carried out at Ogbo stream and its floodplain in Enhwe, Delta StateNigeria. Water samples and fishes were sampled and analyzed using APHA methods and standard fishing gear respectively. The physicochemical parameters revealed distinct values for station 1 (Temperature: 25.63°C, Transparency: 25.09 cm, Sulphate: 7.56 mg/L) and station 2 (Temperature: 23.60 °C, Transparency: 19.90 cm, Sulphate: 6.68 mg/L). Among the parameters, only Temperature, Transparency, and Sulphate exhibited a noteworthy difference (p<0.05) between the two stations. Transparency had a negative correlation with other parameters in Station 1.A total of 725 individual fish species belonging to eight families and genera were sampled. Among the notable findings, Clariidae emerged as the most abundant family, constituting 82.90% of the total relative abundance across both stations. Clarias camerunensisexhibited the highest abundance, comprising 62.20% of the total individuals sampled. Parachanna africanaand Clarias pachynemaalso demonstrated significant presence, contributing 15.00% and 13.20% to the total relative abundance, respectively. ANOVA indicates a significant difference in the composition and abundance of fish species between the stream and floodplain (p < 0.05), emphasizing the influence of habitat-specific factors. The Margalef’s richness (1.67), dominance (0.64), diversity (1.32) and evenness (0.34) index of fishes was higher in the stream than in the floodplains. Magnesium, sulphate, temperature, and transparency were positively correlated to the distribution patterns of Pantodon bucholzi, Clarias jaensis, Phractolaemus ansorgei, and Heterotis niloticusin station 1, with the exception of P. africanaand Polypterus senegalus. There was a negative association between dissolved oxygen, nitrate, pH, and Iron, and the distribution of fishes in station 2. Ogbo stream and its floodplain have low abundance and distribution of fish species. Therefore, it demands for sampling efforts and long-term studies to unravel the checklist of species

Bioremedial Capacity of Indigenous Hydrophytes and Microalgae of Bangladesh: A Comparative Study on their Potential in Tannery Effluent Treatment

Aims: This study aimed to identify the bio-remedial potential of indigenous hydrophytes (Salvinia cucullata and Lemna minor) and microalgae (Spirulina platensis) of Bangladesh for treatment of tannery wastewater disposed in the open environment. Study Design: The capacity of improving the water quality of the tannery effluent by bioremediation was assessed. The quantitative determination included the comparison of physical characters (pH, TDS, EC, DO, COD) and heavy metal profile (Pb, Cr, Cu, Zn) of the tannery effluent before and after treatment. Effluent treatment was carried out by individual species separately and in combination with all for a total of 25 days. Place and Duration of Study: Major experiments were carried out at the Applied Botany Laboratory, Dhaka Laboratory, BCSIR, Dhaka, Bangladesh from January 2022 to February 2024. Quantitative estimations were carried out at Soil & Water Laboratory, Dhaka Laboratory, BCSIR, Dhaka, Bangladesh. Methodology: The tannery effluent was characterized by means of physiochemical parameters and heavy metal concentration prior to the experimental procedure. Four treatment plans were designed, three treatments (TSC, TLM, TSP) using individual species separately and one treatment (TC) using all three species in combination. The treatment continued for 25.0 days. The physiochemical parameters of the treated effluent were measured at 5.0-day intervals and heavy metal conc. Were measured on the 0th, 10th, and 25th days of the experiment. Comparative analysis of the data was utilized to determine useful species for further applied studies in future. Results: After treatments, a reduction at TDS level down to 3230 from 5150 mg/L, and an EC dropdown to 3290 from 10340 μS/cm was observed. Heavy metals removal capacity of the tested species was found as follows, S. cucullata: Zn > Cr >Pb> Cu, L. minor: Pb> Cr > Cu > Zn, S. platensis: Cu > Zn >Pb> Cr, and the combination treatment: Pb> Cu > Zn > Cr. The observation showed high efficiency of S. platensis in TDS reduction and Zn absorption, L. minor in COD reduction and Pb absorption, and S.cucullata in Zn absorption. Conclusion: The comparative data suggested the overall improvement of the effluent quality can be achieved by a combined treatment of L. minor and S. platensis, quickly and cost-efficiently. Further investigation is required for an in-depth understanding of their combined potential. Additionally, investigation will be carried out on their potential in Arsenic (As) removal and nano-particle production from post-treatment biomass.

Evaluation of the effects of dust pollution on specific plant species near and around the marble mining site in Rajasthan, India.

Airborne particles (dust pollution) pose a significant threat to both human and plant populations. Plant leaves act as crucial biofilters, capturing significant amounts of air pollution; this characteristic offers a valuable tool to measure local pollution levels and assess individual plant species' ability to intercept and mitigate harmful dust particles. The present study was carried out to asses the effect of responses of various plant species to dust pollution near and around the marble mining site comprising residential site, highway area, and Central University of Rajasthan as control. The anticipated pollution index, air pollution tolerance index (APTI), dust absorption capacity, metal accumulation index (MAI), and biochemical factors were used to evaluate plant responses. Azadirachta indica A. Juss. demonstrated the highest (29.0) and Vachellia nilotica L. showed lowest (5.6) APTI, respectively. A. indica showed maximum MAI values in comparison to other plant species situated at residential site. Additionally, monitoring of particulate matter (PM10) observed to highest at highway, followed by mining, residential, and control sites. Overall A. indica representing highest APTI and effective dust capturing capacity at all sites could serve as potential pollution sinks. V. nilotica, with its very low APTI, can be marked as biomonitoring tool for detecting dust pollution.

Tafazzin deficiency causes substantial remodeling in the lipidome of a mouse model of Barth Syndrome cardiomyopathy

Barth Syndrome (BTHS) is a rare X-linked disease, characterized clinically by cardiomyopathy, skeletal myopathy, neutropenia, and growth retardation. BTHS is caused by mutations in the phospholipid acyltransferase tafazzin (Gene: TAFAZZIN, TAZ). Tafazzin catalyzes the final step in the remodeling of cardiolipin (CL), a glycerophospholipid located in the inner mitochondrial membrane. As the phospholipid composition strongly determines membrane properties, correct biosynthesis of CL and other membrane lipids is essential for mitochondrial function. Mitochondria provide 95% of the energy demand in the heart, particularly due to their role in fatty acid oxidation. Alterations in lipid homeostasis in BTHS have an impact on mitochondrial membrane proteins and thereby contribute to cardiomyopathy. We analyzed a transgenic TAFAZZIN-knockdown (TAZ-KD) BTHS mouse model and determined the distribution of 193 individual lipid species in TAZ-KD and WT hearts at 10 and 50 weeks of age, using electrospray ionization tandem mass spectrometry (ESI-MS/MS). Our results revealed significant lipid composition differences between the TAZ-KD and WT groups, indicating genotype-dependent alterations in most analyzed lipid species. Significant changes in the myocardial lipidome were identified in both young animals without cardiomyopathy and older animals with heart failure. Notable alterations were found in phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysophosphatidylethanolamine (LPE), lysophosphatidylcholine (LPC) and plasmalogen species. PC species with 2–4 double bonds were significantly increased, while polyunsaturated PC species showed a significant decrease in TAZ-KD mice. Furthermore, Linoleic acid (LA, 18:2) containing PC and PE species, as well as arachidonic acid (AA, 20:4) containing PE 38:4 species are increased in TAZ-KD. We found higher levels of AA containing LPE and PE-based plasmalogens (PE P-). Furthermore, we are the first to show significant changes in sphingomyelin (SM) and ceramide (Cer) lipid species Very long-chained SM species are accumulating in TAZ-KD hearts, whereas long-chained Cer and several hexosyl ceramides (HexCer) species accumulate only in 50-week-old TAZ-KD hearts These findings offer potential avenues for the diagnosis and treatment of BTHS, presenting new possibilities for therapeutic approaches.

Synthesis, characterization, and photocatalytic application of cadmium(II) sulfide-magnetite in one-pot multi-component synthesis of some 1-amidoalkyl-2-naphthols

Cadmium(II) sulfide-magnetite is disclosed as a new magnetic separable photocatalyst in the one-pot multi-component synthesis of 1-amidoalkyl-2-naphthols, as significant pharmaceuticals that have diverse biological activities including antifungal, anti-inflammatory, antioxidant, and antidiabetic properties. This novel and environmental-friendly photocatalyst is biosynthesized from C. Cretica L. leaf extract and characterized by FT-IR, XRD, FE-SEM, EDS, XPS, UV–Vis, and VSM analytical techniques. Results exhibited that the nano-photocatalyst has significantly higher photocatalytic activity compared to individual components Fe3O4 and CdS. This property would be corresponded to retarded recombination of hole-electron pairs in this nanomaterial compared to the individual species. The effective species like OH•, •O2−, and h+ are proposes to be involved in the photocatalytic process. This new green method brings several key benefits like quick reaction time, easy work-up, cost-effectiveness, wide range of applicable substrates, and high yields.

Supplementing narasin or monensin to control coccidiosis in naturally infected calves

Abstract This experiment compared narasin and monensin as anticoccidials for calves naturally infected with Eimeria spp. Twenty-four weaned, non-castrated male calves (Bos indicus × B. taurus cross) were assigned to this experiment (d -8 to 42). All calves were infected by Eimeria spp. according to oocyst count per gram (OPG) from fecal samples collected on d -8 and -7 (average 1,059 ± 101 oocysts/g). Calves were housed in individual pens, received corn silage, mineral mix and water for ad libitum consumption, in addition to a grain-based supplement at 200 g/head daily. Fecal samples were collected on d -2 and -1 for OPG, and results averaged as initial OPG value. Calves were blocked according to initial OPG into 8 blocks of 3 calves each, ranked within each block according to BW recorded on d -1, and assigned to receive narasin (NAR; 0.8 mg/kg of BW), monensin (MON; 1 mg/kg of BW), or no ionophore (CON; negative control). Ionophores were added to the grain-based supplement, and offered from d 0 to 42 of the experiment. Calf BW was recorded on d 7, 14, 21, 28, 35 and 42. Fecal samples were collected on d 6 and 7, 13 and 14, 20 and 21, 26 and 27, 34 and 35, 41 and 42 for OPG analysis, and results from samples collected in consecutive days were averaged. Aliquoted fecal samples were also pooled across calves from the same treatment and collection days, and used to determine prevalence of individual species of Eimeria. No treatment effects were detected (P ≥ 0.51) for calf BW or growth rate. A treatment × day interaction was detected (P < 0.01) for OPG, as NAR and MON calves had less (P < 0.01) OPG compared with CON calves beginning on d 7. The OPG was also less (P ≤ 0.03) in MON compared with NAR calves on d 7, 14, and 28, but did not differ (P ≥ 0.48) on d 21, 35 and 42. The anticoccidial efficacy of NAR and MON did not differ (P ≥ 0.16) when calculated across all Eimeria spp., or according to prevalence of E. bovis and E. alabamensins. A treatment × day interaction was detected (P = 0.04) for anticoccidial efficacy to E. alabamensis, which was greater (P < 0.01) in MON calves on d 7 and 14 and did not differ (P ≥ 0.40) afterwards. Collectively, both ionophores were similarly effective in controlling coccidiosis upon completion of the 42-d study, although the anticoccidial effects of monensin were noted earlier in the experiment. Nonetheless, these results corroborate narasin as an efficient anticoccidial ionophore for naturally infected calves.

Combined application of entomopathogenic fungi and predatory mites for biological control of Tetranychus urticae on chrysanthemum.

Most studies on efficacy of fungal pathogens and predatory mites against Tetranychus urticae have been done on individual species in the laboratory. We evaluated fungi and predatory mites separately and together against glasshouse populations of T. urticae on chrysanthemum plants. First, effectiveness of the fungal pathogens Beauveria bassiana (Bb88) and Metarhizium anisopliae (Ma129) was compared; then, effectiveness of the predatory mites Phytoseiulus persimilis and Neoseiulus californicus. Based on the results, N. californicus and isolate Ma129 were selected and evaluated in combination. In all experiments, treatment effects were assessed for eggs and motile stages of T. urticae. The first experiment showed no significant effect of either fungal isolate on T. urticae populations, except on plants initially infested with 20 mites, where more eggs were found in the control compared to the fungal treatments. In the second experiment, both predatory mites were equally effective at reducing T. urticae populations compared with the control, regardless of initial T. urticae population density. The last experiment demonstrated that populations of T. urticae were reduced most when M. anisopliae (Ma129) and N. californicus were applied together, compared with the control and when each natural enemy was applied separately. Metarhizium anisopliae (Ma129) and B. bassiana (Bb88) isolates did not have a significant effect on reducing T. urticae populations. Both predatory mites reduced T. urticae populations, regardless of T. urticae density. Combined application of M. anisopliae (Ma129) and N. californicus were more effective against T. urticae than the control or when each agent was applied separately. © 2024 Society of Chemical Industry.

Design and Synthesis of 1,3-Diarylpyrazoles and Investigation of Their Cytotoxicity and Antiparasitic Profile.

Herein, we report a series of 1,3-diarylpyrazoles that are analogues of compound 26/HIT 8. We previously identified this molecule as a 'hit' during a high-throughput screening campaign for autophagy inducers. A variety of synthetic strategies were utilized to modify the 1,3-diarylpyrazole core at its 1-, 3-, and 4-position. Compounds were assessed in vitro to identify their cytotoxicity properties. Of note, several compounds in the series displayed relevant cytotoxicity, which warrants scrutiny while interpreting biological activities that have been reported for structurally related molecules. In addition, antiparasitic activities were recorded against a range of human-infective protozoa, including Trypanosoma cruzi, T. brucei rhodesiense, and Leishmania infantum. The most interesting compounds displayed low micromolar whole-cell potencies against individual or several parasitic species, while lacking cytotoxicity against human cells.

What drives growth responses of nitrogen and phosphorus (co-)limited primary producer communities?

The growth of autotroph communities is frequently (co-)limited by essential nutrients such as nitrogen (N) and phosphorus (P). Several mechanisms have been proposed to explain the observed co-limitation patterns at different levels of biological organization, especially at the biochemical level for individual species. When considering communities, the presence of different species and functional groups with contrasting physiologies and nutrient requirements leads to a more difficult understanding of the mechanisms involved in nutrient (co-)limitation. To investigate what drives co-limitation patterns and possible underlying mechanisms based on biomass responses in autotroph communities, we grew phytoplankton communities differing in species composition in experimental microcosms on three N:P ratios to impose different limiting conditions. Afterwards, N, P, both, or none were factorially supplied to the communities to test which nutrients were limiting growth. We measured the biovolume of single species in the communities to assess how they responded to nutrient additions and compared it to the response of the overall community biovolume. The types of nutrient (co-)limitation identified, i.e. the factorial limitation scenarios for community biomass were single N limitation or simultaneous co-limitation by N and P, and were strongly driven by the dominant species. The phytoplankton species in the communities responded differently to the nutrient addition treatments, i.e. they showed contrasting limitation outcomes and therefore likely different nutrient requirements. Our experiment indicates that phylogenetically distantly-related phytoplankton species grown in a community can have different resource use efficiencies and thus can be limited by different nutrients. We suggest that the dominance of species or groups with similar traits in nutrient requirements and acquisition is one of the leading mechanisms that determines the biomass pattern of nutrient (co-)limitation observed at the community level. This work also highlights the potential of predicting community growth limitation outcomes based on knowledge of nutrient use efficiencies of one or few dominant species, which can be a suitable tool for lake restoration and oligotrophication efforts.

Arthropod Diversity of Municipal Solid Waste (MSW) in Ebonyi Waste to Wealth Company Abakaliki Ebonyi State, Nigeria

The study was designed to determine the diversity of arthropods in Ebonyi State waste to wealth Abakaliki, a municipal waste site (MWS) in Abakaliki where solid wastes of all sorts are deposited. The arthropod specimens were analysed using standard entomological technique of sweep net, pitfall traps and malaise trap respectively. Arthropod species identification was done morphologically at insect Museum Ahmed Bello University in Zaria. A total of 2000 arthropod specimens were collected comprising 8 orders, 18 families and 28 species. The most abundant species in diversity is Bengalia gallard 208 (10.4) followed by Musca domestica 205(10.2). Out of the 2000 individual species of arthropods collected from MSW dump site in Abakaliki, Diptera (48.30) and Hymenoptera (39.12) were the most prevalent while Dermaptera were the least in abundance. Four insect species were heterometabolic including medically important species, Blattela sp. (Blattodea). Insect Order Diversity in Respect to traps used for collection showed that pitfall trap with the total of 854 insects recorded the highest and Hymenoptera (43.2) recording the highest frequency. Shannon Weiner's Diversity Index of Insect Orders revealed that Diptera (0.48) had the highest diversity. Determination of the arthropod diversity in MSW will help in the control of possible vector borne disease epidemics especially in areas where there is no awareness of disease to its vector transmission.

Phytophthora Communities Associated with Agathis australis (kauri) in Te Wao Nui o Tiriwa/Waitākere Ranges, New Zealand

Studies of Phytophthora impact in forests generally focus on individual species without recognition that Phytophthora occur in multispecies communities. This study investigated community structure of Phytophthora species in the rhizosphere of Agathis australis (kauri) in Te Wao Nui o Tiriwa/Waitākere Ranges, New Zealand, in the context of kauri dieback disease expression. Soil sampling and tree monitoring were conducted on 767 randomly selected mature kauri trees. Phytophthora species were detected using both soil baiting and DNA metabarcoding of environmental DNA (eDNA). Four species were detected with soil baiting (P. agathidicida, P. cinnamomi, P. multivora, and P. pseudocryptogea/P. cryptogea) and an additional three species with metabarcoding (P. kernoviae, P. cactorum/P. aleatoria and an unknown clade 7 species). Phytophthora cinnamomi was the most abundant species and was distributed throughout the forest. Both P. multivora and P. agathidicida were limited to forest edges, suggesting more recent introductions. P. agathidicida presence was strongly correlated with declining canopy health, confirming its role as the main driver of kauri dieback. The limited distribution of P. agathidicida and infrequent detections (11.0% samples) suggests that that this species is spreading as an introduced invasive pathogen and provide hope that with strategic management (including track upgrades and closures, restricting access to uninfected areas, and continual monitoring) uninfected areas of the forest can be protected. The frequent detections of P. cinnamomi and P. multivora from symptomatic trees in the absence of P. agathidicida suggest more research is needed to understand their roles in kauri forest health.

Wood Basic Density in Large Trees: Impacts on Biomass Estimates in the Southwestern Brazilian Amazon

Wood basic density (WD) plays a crucial role in estimating forest biomass; moreover, improving wood-density estimates is needed to reduce uncertainties in the estimates of tropical forest biomass and carbon stocks. Understanding variations in this density along the tree trunk and its impact on biomass estimates is underexplored in the literature. In this study, the vertical variability of WD was assessed along the stems of large trees that had a diameter at breast height (DBH) ≥ 50 cm from a dense ombrophilous forest on terra firme (unflooded uplands) in Acre, Brazil. A total of 224 trees were sampled, including 20 species, classified by wood type. The average WD along the stem was determined by the ratio of oven-dry mass to saturated volume. Five models were tested, including linear and nonlinear ones, to fit equations for WD, selecting the best model. The variation among species was notable, ranging from 0.288 g cm−3 (Ceiba pentandra, L., Gaertn) to 0.825 g cm−3 (Handroanthus serratifolius, Vahl., S. Grose), with an average of 0.560 g cm−3 (±0.164, standard deviation). Significant variation was observed among individuals, such as in Schizolobium parahyba var. amazonicum (H. ex D.), which ranged from 0.305 to 0.655 g cm−3. WD was classified as low (≤0.40 g cm−3), medium (0.41–0.60 g cm−3), and high (≥0.61 g cm−3). The variability in WD along the stem differs by wood type. In trees with low-density wood, density shows irregular variation but tends to increase along the stem, whereas it decreases in species with medium- and high-density wood. The variation in WD along the stem can lead to underestimations or overestimations, not only in individual trees and species but also in total stocks when estimating forest biomass. Not considering this systematic bias results in significant errors, especially in extrapolations to vast areas, such as the Amazon.