In this study, a thermal-diffusive model using a sinusoidal function for the oscillation term is presented. The model is employed to evaluate the pulsating behavior in a premixed counterflow flame fed with biomass particle cloud considering Lewis number and thermal radiation effects. In order to scrutinize the pulsating characteristics of a flame, time-related governing equations are written for the assumed multi-zone system and analytically solved utilizing Matlab and Mathematica software. To enforce continuity, suitable matching conditions for the temperature and heat flux at the interfaces are derived. Time-dependent characteristics of burning velocity, flame temperature, flame front position, reactants temperature, and mass distributions are presented, taking into account effective Lewis number, thermal radiation, and strain rate influences. An acceptable comparison with numerical simulations affirms the reliability of the mathematical procedure. The obtained results indicated that the pulsating instability of biomass-fueled premixed flames in counterflow arrangements significantly relevant not only to the effective Lewis number but also to thermal radiation transferred from the reaction zone to unburnt reactants. Furthermore, it is found that in counterflow premixed flames, strain rate, in addition to Lewis number and thermal radiation, can be the key to controlling the individual flame oscillation modes.