In recent years the risk of transferring vancomycin-resistant enterococci (VRE) from animals to humans has caused great concern. Studies have shown that the glycopeptide growth promoter avoparcin selects for VRE (1, 3), and high numbers have been found in food animals and foodstuff in Europe (4, 7, 10). In one case, identical strains were isolated from both a turkey farmer and his turkeys (9). The finding of VRE in nonhospitalized humans and in meat eaters but not in vegetarians has further indicated a food-associated spread of vancomycin resistance from animals to humans (8). Most of the vancomycin-resistant Enterococcus faecium (VREF) strains isolated in Europe contain Tn1546. This transposon was first isolated from E. faecium BM4147 (2). Studies of Tn1546 have revealed that only the vanA, vanH, and vanX genes are essential for resistance (Fig. (Fig.1).1). Characterization of Tn1546-like elements in isolates of animal and human origin indicated only minor variations caused by inseration sequences outside the essential part of Tn1546 (Fig. (Fig.1),1), and a base pair variation in the vanX gene was discovered at position 8234 (5). In this position either a G (G type) or a T (T type) was found (Fig. (Fig.1).1). Based on all these variations, different types of Tn1546-like elements were defined, and indistinguishable elements were found in isolates of human and animal origin (5). FIG. 1 The Tn1546 encoding vancomycin resistance. The sizes and positions of the genes are indicated. The genes are grouped into the categories Mobility, Regulation, Essential, and Accessory according to their importance for vancomycin resistance. The positions ... In this study a total of 271 VREF isolates of animal (226) and human (45) origin were investigated for this base pair variation. All animal isolates originated from different herds, and all human isolates, except strains from Norway and Saudi Arabia, have previously been typed to independent clones (4–6). PCR amplification of a 424-bp amplicon of the vanX gene of Tn1546 (Fig. (Fig.1)1) was obtained from all isolates, confirming the presence of Tn1546-like elements. By digesting the amplicons with the restriction enzyme DdeI, two distinct patterns of fragments were obtained for the G and T types. Based on these results the distribution of the base pair variation could thus be ascertained. As evident from Table Table1,1, all isolates from poultry belonged to the G type, while 32 of the 33 porcine isolates belonged to the T type. In human isolates both variations were found but only one of the variants dominated locally. Only the G type was found in isolates from England, Norway, and Saudi Arabia, thus associating these isolates with poultry. For the human Nowegian isolates this could be expected, since they were isolated from poultry farmers. All Danish human VREF isolates belonged to the T type, thereby associating them with pigs. TABLE 1 Variations in Tn1546-like elements of VREF isolates of animal and human origin The present study showed that VREF isolates from pigs, poultry, and humans could be divided according to base pair variation in the vanX gene at position 8234 (G or T type). All poultry isolates belonged to the G type, whereas almost all porcine isolates, except one Danish isolate, belonged to the T type. This finding indicates, with particular reference to the Danish isolates, that horizontal exchange of VREF isolates or Tn1546-like elements between poultry and pigs does not occur frequently. On the other hand, both types were found among humans, indicating that humans may be infected from both sources. Based on this observation it may be hypothesized that the primary transmission is from animals to humans and not the other way around. However, further studies are required to test this assertion. The present results indicate that the base pair variation in the vanX gene may be a useful marker for epidemiological studies on the spread of VREF isolates and vancomycin resistance genes.