Indigenous Trichoderma Research Articles

Isolation, screening, and molecular characterization of indigenous Trichoderma isolates from West Java, Indonesia and their plant growth-promoting capability on rice plants (Oryza sativa L.)

Trichoderma species are widely acknowledged as growth-promoting fungi that have been utilized to enhance the growth and yield of numerous crops. This research examined the capacity of 30 Trichoderma strains, isolated from two organic rice fields in West Java, Indonesia, to enhance the germination, growth, and physiological characteristics of rice plants (Oryza sativa L.). In general, Trichoderma strain TM10 demonstrated the greatest ability to increase seed germination (97.25 %), vigor index (3122.83), and germination speed (59.91 seeds/day). This strain also increased seedling root length and seedling height by 101.62 % and 112.20 %, respectively. Plants treated with TM10 exhibited a notable improvement in root length, plant height, fresh weight, and dry weight compared to control plants, demonstrating increases of 188.68 %, 69.90 %, 157.41 %, and 159.38 %, respectively. Furthermore, the total chlorophyll content and stomatal number in TM10-treated plants exhibited increments of 75.23 % and 75.53 %, respectively. Five selected isolates (TM7, TM10, SB2, SB8, and SB14) were evaluated for their potential to produce plant growth-promoting compounds, including phosphatase enzyme (ranging from 0.54 to 11.14 µg pNP g−1h−1), indole-3-acetic acid (IAA) (ranging from 28.96 to 63.91 µg/mL), ammonia (ranging from 1.96 to 5.79 µg/mL), and hydrogen cyanide (HCN) (ranging from 221.76 to 274.82 ppm). The best strain, namely TM10, was then molecularly identified as Trichoderma yunnanense. This investigation demonstrates that Trichoderma spp. isolated from organic rice fields could be used as a bioinoculant in sustainable rice production.

Trichoderma carraovejensis: a new species from vineyard ecosystem with biocontrol abilities against grapevine trunk disease pathogens and ecological adaptation.

Trichoderma strains used in vineyards for the control of grapevine trunk diseases (GTDs) present a promising alternative to chemical products. Therefore, the isolation and characterization of new indigenous Trichoderma strains for these purposes is a valuable strategy to favor the adaptation of these strains to the environment, thus improving their efficacy in the field. In this research, a new Trichoderma species, Trichoderma carraovejensis, isolated from vineyards in Ribera de Duero (Spain) area, has been identified and phylogenetically analyzed using 20 housekeeping genes isolated from the genome of 24 Trichoderma species. A morphological description and comparison of the new species has also been carried out. In order to corroborate the potential of T. carraovejensis as a biological control agent (BCA), confrontation tests against pathogenic fungi, causing various GTDs, have been performed in the laboratory. The compatibility of T. carraovejensis with different pesticides and biostimulants has also been assessed. This new Trichoderma species demonstrates the ability to control pathogens such as Diplodia seriata, as well as high compatibility with powdered sulfur-based pesticides. In conclusion, the autochthonous species T. carraovejensis can be an effective alternative to complement the currently used strategies for the control of wood diseases in its region of origin.

Exploration and characterization of indigenous Trichoderma spp. as antagonist of Rhizoctonia solani and plant growth promoter of maize

Exploration and characterization of indigenous Trichoderma spp. as antagonist of Rhizoctonia solani and plant growth promoter of maize

Exploring the antagonist potential of indigenous Trichoderma spp., Bacillus, and Pseudomonas against Phytophthora palmivora of Soe mandarin in East Nusa Tenggara, Indonesia

Phytophthora palmivora-induced root and basal stem rot pose a significant threat to the survival of Soe mandarin plants in East Nusa Tenggara Province, Indonesia. We isolated four Trichoderma species, five Bacillus, and 13 Pseudomonas isolates from the rhizosphere of healthy Soe mandarin. This study pursued two main objectives: (a) assessing the inhibitory capabilities of indigenous Trichoderma spp., Bacillus, and Pseudomonas towards P. palmivora in vitro, and (b) investigating the combined efficacy of Trichoderma spp., Bacillus, and Pseudomonas in controlling P. palmivora in a pot trial. Results revealed that four Trichoderma species (T. asperellum, T. hamatum, T. harzianum, and T. viride) significantly inhibited the growth of P. palmivora. Notably, T. asperellum exhibited the highest inhibition, at 84.31%, followed closely by T. harzianum (84.11%), T. viride (83.67%), and T. hamatum (80.28%). Bacillus and Pseudomonas exhibited varying levels of inhibition to P. palmivora growth in vitro, with the most substantial inhibition observed in Bacillus 1, followed by Pseudomonas 6. In the subsequent pot trial, the application of Trichoderma, Bacillus, or Pseudomonas, either individually or in combination, significantly increased the height increment and reduced the disease incidence caused by P. palmivora in Soe mandarin seedlings.

Biocontrol efficacy and induced defense mechanisms of indigenous Trichoderma strains against Fusarium wilt [F. udum (Butler)] in pigeonpea

This study aimed to identify efficient Trichoderma isolate(s) for the management of Fusarium wilt in pigeonpea. In vitro and in vivo (greenhouse) experiments were conducted to evaluate 17 Trichoderma isolate(s) for their anti-pathogenic activity, induction of plant biochemical functions, disease suppression, and plant growth traits comparing with standard Trichoderma strains. Among the indigenous isolates, IIPRTh-31 (T. asperellum) and IIPRTh-33 (T. afroharzianum) had a higher mycelia growth inhibition in vitro [membrane assay (>74%), binary assay (>65%)]. Seed treatment with IIPRTh-31 and IIPRTh-33 isolates led to an increase in peroxidase (173–244%), polyphenol oxidase (48–80%), phenylalanine ammonia–lyase (147–178%) activities in pigeonpea plants over control and reduced disease incidence by 78–85%. Hence, IIPRTh-31, and IIPRTh-33 strains demonstrate excellent bio-control of Fusarium wilt in pigeonpea via induction of defense-related enzymes. These isolates have been registered (Registration No. NAIMCC-R-5, NAIMCC-R-6) and used for talc-based bio-formulations for field application.

Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF

Soil pollution with heavy metals is a serious threat to the environment. However, soils polluted with heavy metals are considered good sources of native metal-resistant Trichoderma strains. Trichoderma spp. are free-living fungi commonly isolated from different ecosystems, establishing endophytic associations with plants. They have important ecological and biotechnological roles due to their production of a wide range of secondary metabolites, thus regulating plant growth and development or inducing resistance to plant pathogens. In this work we used indigenous Trichoderma strains that were previously isolated from different soil types to determine their tolerance to increased copper and nickel concentrations as well as mechanisms of metal removal. The concentrations of bioavailable metal concentrations were determined after extraction with diethylene-triamine pentaacetate (DTPA)-extractable metals (Cd, Cr, Co, Cu, Pb, Mn, Ni, and Zn) from the soil samples by inductively coupled plasma-optical emission spectrometry (ICP-OES). Two indigenous T. harzianum strains were selected for copper tolerance, and three indigenous T. longibrachiatum strains were selected for nickel tolerance tests. Strains were isolated from the soils with the highest and among the lowest DTPA-extractable metal concentrations to determine whether the adaptation to different concentrations of metals affects the mechanisms of remediation. Mechanisms of metal removal were determined using Fourier-transform infrared spectroscopy (FTIR) and X-ray fluorescence spectroscopy (XRF), non-destructive methods characterized by high measurement speed with little or no need for sample preparation and very low costs. Increased DTPA-extractable metal content for nickel and copper was detected in the soil samples above the target value (TV), and for nickel above the soil remediation intervention values (SRIVs), for total metal concentrations which were previously determined. The SRIV is a threshold of metal concentrations indicating a serious soil contamination, thus confirming the need for soil remediation. The use of FTIR and XRF methods revealed that the presence of both biosorption and accumulation of metals in the Trichoderma cells, providing good bioremediation potential for Ni and Cu.

Evaluating the Biocontrol potential of Indigenous Trichoderma spp. against soil borne pathogens of rice and their impact on rhizospheric functional microbial populations

Trichoderma spp. is naturally mycoparasite and the most widely used biocontrol agent (BCA). This Trichoderma spp. is also naturally growth enhancer in crop plants. However, their uses in rice plants are limited to few reports only. The use of excessive Trichoderma as BCA may have effect on soil or soil microflora. We have also characterized Trichoderma spp. biochemically especially for their chitinase and protease activity. The impact of Trichoderma spp. showed that Trichoderma inoculation increases the growth of specific bacterial population and decreases the soil fungal population in rice soil, which indicated that the isolates may the best potential strains to control the soil pathogens. Antagonism effect of isolated Trichoderma spp. was found effective against various soil borne pathogens like R. solani, S. oryzae than S. rolfsii.

Isolation, Evaluation and Characterization of Indigenous Trichoderma spp. for the Management of Wilt of Chickpea Caused by Fusarium oxysporum f. sp. ciceris

Background: Fusarium oxysporum f. sp. ciceris causing chickpea wilt, a significant soil-borne disease that causes severe crop losses. Trichoderma spp. is a diverse fungal bio-control agent with the ability to limit plant disease growth through a variety of ways. The goal of this work is to assess the efficiency of indigenous Trichoderma spp. isolated from chickpea rhizosphere from different North Eastern districts of Karnataka against the wilt pathogen in vitro, as well as to investigate the cultural and morphological aspects of isolates. Methods: Dual culture approach was used to investigate the antagonistic potential of Trichoderma isolates and inverted plate technique was used to examine the synthesis of volatile compounds from Trichoderma spp. The isolates were cultured on potato dextrose agar medium for cultural characterization. Under a microscope, morphological characters were observed. Result: Twenty Trichoderma isolates were reported to be effective against F. oxysporum f. sp. ciceris, preventing mycelial growth by 48.52 to 91.85%. All the isolates produced significant amount of volatile compounds that inhibited F. oxysporum f. sp. ciceris growth by 2.59 to 72.22%. At 72 hours after incubation, the isolates had different radial mycelium growth and formed fluffy and elevated colony growth, with colony margins ranging from smooth to wavy.

Equilibrium kinetics and thermodynamic studies on biosorption of heavy metals by metal-resistant strains of Trichoderma isolated from tannery solid waste.

This study was aimed at finding the metal sorption potential of six indigenous Trichoderma strains by using batch experiments for Cd (II), Cr (VI), Cu (II), and Pb (II). Trichoderma atrobrunneum showed maximum metal biosorption potential at 800mgL-1 of initial concentration. Two adsorption isotherm models, (1) Langmuir (2) Freundlich models, were employed on the biosorption data obtained at various initial metal concentrations (10mgL-1-200mgL-1) and pseudo-first (PSI) and pseudo-second (PSII) order equilibrium kinetic models were subjected to data of agitation time (3-7days). A maximum correlation coefficient value (R2) of ≤ 1 was observed for the Langmuir and PSII model. Results revealed that pH 6-7 was the best for metal sorption, while metal removal efficiency was increased by increasing temperature (298K, 303K, 308K, 313K). The results of thermodynamic study parameters (∆G°, ∆H°, ∆S°) indicated that heavy metal biosorption by Trichoderma strains was an endothermic, spontaneous, and feasible process. Moreover, surface characterization analysis through SEM, BET, FTIR, and XRD showed that T. atrobrunneum and Trichoderma sp. could adsorb more metal ions when grown in high metal concentrations. The results indicate that living biomass of T. atrobrunneum and Trichoderma sp. is an effective multi-metal biosorbent that can be used for efficacious bioremediation of bio-treatment of heavy metal polluted wastewater.

Characterization and evaluation of bioformulation from antagonistic and flower inducing Trichoderma asperellum isolate UCRD5

Trichoderma species due to their fungal plant pathogen suppression and ecofriendly nature are Trichoderma species due to their fungal plant pathogen suppression and ecofriendly nature are deployed in agriculture as biocontrol agents for the management of plant diseases in agricultural crops since a long-time. The potential biocontrol species/strains of Trichoderma from native geographic locations are often used for the effective management of various abiotic and biotic stresses in plants. Furthermore, bioformulation of biocontrol strains are often prepared using different carriers and evaluated for their effectiveness. Therefore, the isolates of Trichoderma were evaluated against plant fungal pathogens such as Fusarium oxysporum, Sclerotinia sclerotiorum, Alternaria solani and Phytophthora sp. suppression. The pot experiments carried in tomato plants inoculated with a combination of pathogens and one of the most effective Trichoderma asperellum isolate UCRD5 revealed that beside disease suppression, the biocontrol strain led to the significantly early induction of flowering in plants, compared to control. Further, characterization of talc powdered and calcium alginate based bioformulation of Trichoderma asperellum isolate revealed better shelf-life for calcium alginate based bead bioformulation. Keeping in view the difference in the biocontrol potential of Trichoderma isolates from different geographic locations, the present study explores the native isolates of Trichoderma from the agricultural fields of Punjab region, for the effective management of various abiotic and biotic stresses in agricultural crops. Further, efforts were made to enhance the shelf-life and biocontrol efficacies using sodium alginate based beads. The ability of the indigenous Trichoderma isolated to suppress plant fungal pathogens and induce flowering in tomato, coupled to encapsulated formulations with better shelf-life indicates its potential in enhancing the agricultural productivity.

Efficacy of indigenous Trichoderma isolates of West Timor, Indonesia, as biocontrol agents of brown spot (Drechslera oryzae) on two upland rice varieties

BackgroundBrown spot disease is one of the most destructive foliar diseases of rice. Biological control of this disease is considered more environmentally safe, but the biological control agents have limited availability. The fungus Trichoderma can be used as a biocontrol agent for various plant diseases, including brown spots. Indigenous Trichoderma species from West Timor were evaluated in this study to reveal their effectiveness in reducing brown spot disease of upland rice. The study was conducted under a screen house condition using a factorial treatment design laid out in a completely randomized design. The two factors were the brown spot disease control method (T. viride, T. harzianum, T. hamatum, fungicide Trivia 73 WP, and a control treatment) and upland rice genotype (Inpago 7 and Breun Senaren 2).ResultsThe results showed that Trichoderma spp. and the fungicide Trivia 73 WP caused significantly lower AUDPC and apparent infection rate than the control treatment. The total phenol content and agronomic characters (number of reproductive tillers per plant, number of filled grains per panicle, grain yield per plant) were higher in the Trichoderma and fungicide treatments than the control treatment. Meanwhile, tannin and saponin levels were non-significantly different among treatments. Trichoderma and the fungicide Trivia treatments effectively suppressed brown spot disease of upland rice, and the efficacy was dependent on the rice variety, with a mean efficacy range over the two varieties of 27.05–36.42%. The Trichoderma and the fungicide Trivia treatments increased the grain yield per plant differentially in the 2 rice varieties, with a mean grain yield increase over the 2 varieties of 13.0% (T. viride), 23.5% (T. harzianum), 35.0% (T. hamatum), and 19.9% (Trivia 73 WP).ConclusionEfficacy of local Trichoderma of West Timor and the fungicide Trivia treatments were varietal dependent with T. hamatum being the most effective in reducing the brown spot disease and producing the highest grain yield increase over the 2 rice varieties. The local Trichoderma species of West Timor have a future potential as biocontrol agents of upland rice brown spot disease, but it is necessary to further evaluate their effectiveness against the disease in replicated field trials.

Screening and biocontrol evaluation of indigenous native Trichoderma spp. against early blight disease and their field assessment to alleviate natural infection

BackgroundEarly blight disease of tomato caused by pathogenic fungi Alternaria solani is the most significant and common disease throughout the world as well as in Kingdom of Saudi Arabia. The aim of this study was to isolate and identify native Trichoderma species from the Jeddah region in Saudi Arabia; evaluate their antagonistic potential against A. solani; and study their influence early blight disease severity in greenhouse and in open field.ResultsThe present study focused to explore the biocontrolling potential of native Trichoderma spp. against A. solani strain to compare with a conventional fungicide. Out of 21, 3 Trichoderma isolates showed an antifungal activity and significantly inhibited the mycelial growth of pathogen that were identified as Trichoderma atroviride, T. harzianum and T. longibrachiatum by their ITS region sequence analysis. Strong in vitro mycelial growth suppression (70.66%) was also recorded at 400 ppm Mancozeb (90%WP®) fungicide. Further, these Trichoderma bioagents and fungicide were further evaluated in greenhouse (artificially inoculated) and in field on naturally infected tomato plants. In greenhouse, (13.74%) disease severity after T. harzianum treatment was recorded, followed by T. longibrachiatum (25.83%) and T. atroviride (21.67%). The disease severity after fungicide (50 mg/L; 10 ml per plant) application was (7.91%). Further, positive impact on the plant biomarkers was demonstrated by all selected Trichoderma isolates in greenhouse. Under natural infection in season I, the disease severity (%) after T. longibrachiatum, T. atroviride and T. harzianum treatments was 11.5, 13.26 and 16.81%, respectively, followed by control (32.12%), whereas 7.18% disease severity was recorded after fungicide application.ConclusionsThe results revealed that native Trichoderma of this region had potential to mitigate the early blight disease intensity in field.

Comparative Performance of Fungicides and Biocontrol Products inthe Management of Fusarium Wilt of Blackberry.

Fusarium wilt of blackberry (FWB) is an emerging disease caused by a Fusarium oxysporum species complex. More than 3,000 ha of blackberry (Rubus spp.) crops have been lost in Mexico since 2011. The objectives of this research were: to evaluate the sensitivity of pathogenic F. oxysporum isolates recovered from symptomatic blackberry plants to fungicides with different modes of action; to assess the potential of these fungicides and plant resistance inducers against FWB in the greenhouse; and to determine the effects of commercial biofungicides and two indigenous strains of Trichoderma spp. on the incidence of FWB. The EC50 values of the fungicides prochloraz, thiabendazole, azoxystrobin, thiophanate-methyl, difenoconazole, triflumizole, and potassium phosphite for six pathogenic F. oxysporum isolates were determined. In a separate experiment, the fungicides acibenzolar-s-methyl (ASM), potassium phosphite, and commercial biofungicides, as well as two soil microbial inoculants and two indigenous Trichoderma strains, were tested for protection against wilt development in blackberry plants in the greenhouse. Prochloraz showed an average sensitivity for EC50 of 0.01 μg ml-1 for the tested F. oxysporum isolates, followed by difenoconazole and thiabendazole. Prochloraz and ASM proved to be the most effective treatments in the greenhouse. In contrast, potassium phosphite was ineffective in both the invitro and invivo experiments. The soil bioinoculants MicroSoil, Baktillis, T. koningiopsis, and T. asperellum significantly reduced the incidence of disease in the greenhouse. These results provide evidence for the potential of the various tools as useful components of integrated FWB management in the field.

Introduction of indigenous Trichoderma spp. as an induced systemic resistance for Dieback disease control on the Nutmeg seedlings

Dieback disease caused by L. theobromae has destroyed nutmeg plantations in Aceh Selatan. Until now, effective control has not been found, while farmer continue to use pesticides that have a negative impact on the environment. Therefore, biological control is an alternative to environmentally friendly control that is being promoted. The purpose of this researth was to control dieback by inducing resistance using three spesies of Trichoderma (local isolates) from the Nutmeg. The three isolates were isolates of plant origin and the nutmeg seeds used are one year old. This researth used a randomized blok design with three species of Trichoderma (T. virens, T. asperellum, and T. harzianum), three doses (5, 10, 15 g), and three replicates.The variables observed were the incubation period, disease incidence, length of stem discoloration, height of the plant, and plant wet weight. Thr result showed that all species of Trichoderma tested were able to induce systemic resistance and promoted plant growth, by inhibiting the incubation period, disease incidence, length of discoloration, increasing height and weight of plant. The treatment of T. virens 10 g was the best treatment result compared to the others.

Impact of Soil Chemical Properties on the Growth Promotion Ability of Trichoderma ghanense, T. tomentosum and Their Complex on Rye in Different Land-Use Systems.

Microbial-based biostimulants that increase plant performance and ensure sustainable restoration of degraded soils are of great importance. The aim of the present study was to evaluate the growth promotion ability of indigenous Trichoderma ghanense, T. tomentosum and their complex on early rye seedlings in sustained grassland and arable soil. The impact of soil chemical properties on the ability of selected Trichoderma strains and their complex to promote plant growth was determined by the evaluation of the rye (Secale cereale L.) early seedling growth—measuring the length of shoots and roots as well as their dry weight. Trichoderma species were tested for their ability to produce extracellular degradative enzymes on solid media. Furthermore, the soil properties and CM-cellulase activity of soil were estimated. The indigenous Trichoderma strains possess the capacity to produce enzymes such as peroxidase, laccase, tyrosinase, and endoglucanase. The results indicated a significant (p < 0.05) increase in plant growth and the improvement of some soil chemical properties (total N, mobile humic and fulvic acids, exchangeable K2O, soil CM-cellulase activity) in inoculated soils when compared to the control. The growth of the roots of rye seedlings in sustained grassland was enhanced when T. tomentosum was applied (p = 0.005). There was an increase in total weight and shoot weight of rye seedlings when T. ghanense was used in the arable soil (p = 0.014 and p = 0.024). The expected beneficial effect of Trichoderma spp. complex on rye growth promotion was not observed in any tested soil. The results could find application in the development of new and efficient biostimulants, since not only do physiological characteristics of fungi play an important role but also the quality of the soil has an impact.

Biocontrol potentials of novel indigenous Trichoderma isolates against Fusarium wilt of chickpea

Biocontrol potentials of novel indigenous Trichoderma isolates against Fusarium wilt of chickpea

Exploring the indigenous Trichoderma strains from pulses rhizosphere and their biocontrol potential against Fusarium oxysporum f. sp. ciceri in chickpea

Exploring the indigenous Trichoderma strains from pulses rhizosphere and their biocontrol potential against Fusarium oxysporum f. sp. ciceri in chickpea

Pectinolytic potential of indigenous Trichoderma strains originated from the north western regions of Pakistan

Pectinolytic potential of indigenous Trichoderma strains originated from the north western regions of Pakistan

In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

BackgroundFaba bean (Vicia faba L.) cultivation is highly challenged by faba bean black root rot disease (Fusarium solani) in high lands of Ethiopia. To ensure sustainable production of faba beans, searching for eco-friendly disease management options is necessary to curb the progress of the disease timely. The indigenous biocontrol agents that suit local environments may effectively strive with in-situ microorganisms and suppress local pathogen strains. This study aimed to screen antagonistic indigenous compatible Trichoderma and Pseudomonas strains against Fusarium solani. In the pathogenicity test, soil-filled pots were arranged in complete random block design and sown with health faba bean seeds. The effect of some fungicides was evaluated against Fusarium by food poisoning methods to compare with the biocontrol agents. The antagonistic efficacy of biocontrol agents and their compatibility was investigated on Potato dextrose agar medium.ResultsFusarium solani AAUF51 strain caused an intense root rotting in faba bean plant. The effect of Mancozeb 80% WP at 300 ppm was comparable with Trichoderma and Pseudomonas strains against Fusarium. The mycelial growth of test the pathogen was significantly (P ≤ 0.05) reduced to 86.67 and 85.19% by Trichoderma harzianum AAUW1 and Trichoderma viridae AAUC22 strains in dual culture, respectively. The volatile metabolites of Pseudomonas aeruginosa AAUS31 (77.78%) found the most efficient in reducing mycelial growth of Fusarium followed by Pseudomonas fluorescens AAUPF62 (71.11%) strains. The cell-free culture filtrates of Pseudomonas fluorescens AAUPF62 and Pseudomonas aeruginosa AAUS31 were more efficient than the Trichoderma strain in reducing the growth of Fusarium isolates. There was no zone of inhibition recorded between Trichoderma harzianum AAUW1, Trichoderma viridae AAUC22, Pseudomonas aeruginosa AAUS31, and Pseudomonas fluorescens AAUPF62 strains, hence they were mutually compatible.ConclusionsThe compatible Trichoderma and Pseudomonas strains showed antagonistic potentiality that could be explored for faba bean protection against black root rot disease and might have a future dual application as biocontrol agents.

Indigenous Trichoderma harzianum as Biocontrol toward Blight Late Disease and Biomodulator in Potato Plant Productivity

Indigenous Trichoderma has widely used in late-blight epidemic areas in Magelang, Indonesia as biocontrol and biomodulator. But it application still lacks of scientific proof, even though it is potently to be developed. Therefore, this study aims to identify and evaluate indigenous Trichoderma application in eradicating late blight and increase productivity. This research was an experimental posttest-only using six experimental plots, with P0 plot with no treatment. The solution was made by dissolve indigenous Trichoderma solid-starter into water (m/v), then used for treatment. The treatment plots including: two groups of sprayed-only plots with doses of 100 g/ 10 L of solution for P1 and 150 g/ 10 L of solution for P2; and two group treated with sprayed-poured method using doses of 100 g/ 10 L of solution for P3 and 150 g/ 10 L of solution for P4. The positive control group was P5 group, treated using chemical pesticides and tuber booster. The microscopic identification revealed that the local bioagent was Trichoderma harzianum species. Application of T. harzianum in P4 was significantly increased the productivity. However, it was not effective in increasing growth, but able to reduce the intensity of late blight disease. The research revealed that native Trichoderma is able to be used as anti-infectious agent and potentially improve the quality of potato plants. In the future, this research may worthwhile for farmers to develop and produce trustworthy and proven Trichoderma-based biocontrol and help them increase the potato production economically.