Indigenous Bacteria Research Articles

Effects of Inoculation of Thermotolerant Bacillus Strains on Lignocellulose Degradation

Thise study investigated the effect of three lignocellulolytic thermophilic Bacillus strains (F11, Q1, and FP4) on lignocellulose degradation, enzymatic activities, and microbial community structure in composting. The lignin degradation rate reached 36% in the presence of the inoculant, the hemicellulose degradation rate ranged from 43% (F11) to 51% (Q1), and cellulose degradation rates reached 57% in F11 and in FP4, which were significantly higher than the control (CK). The inoculation treatment could explain 28% of the lignin degradation for all three strains. The contribution of FP4 to hemicellulose and cellulose degradation was 30% and 20%, respectively. Compared to CK, lignin peroxidase activity in the water extract of the compost had increased by 66~145% for inoculation treatments, and manganese peroxidase and laccase activity increased by 114% and 78% for Q1. The inoculation stimulated the growth of indigenous bacteria with stronger lignocellulolytic enzyme-producing ability; such shifts in microbial communities were most likely responsible for the improved lignocellulose degradation.

Giardia antagonizes beneficial functions of indigenous and therapeutic intestinal bacteria during protein deficiency

ABSTRACT Undernutrition in children commonly disrupts the structure and function of the small intestinal microbial community, leading to enteropathies, compromised metabolic health, and impaired growth and development. The mechanisms by which diet and microbes mediate the balance between commensal and pathogenic intestinal flora remain elusive. In a murine model of undernutrition, we investigated the direct interactions Giardia lamblia, a prevalent small intestinal pathogen, on indigenous microbiota and specifically on Lactobacillus strains known for their mucosal and growth homeostatic properties. Our research reveals that Giardia colonization shifts the balance of lactic acid bacteria, causing a relative decrease in Lactobacillus spp. and an increase in Bifidobacterium spp. This alteration corresponds with a decrease in multiple indicators of mucosal and nutritional homeostasis. Additionally, protein-deficient conditions coupled with Giardia infection exacerbate the rise of primary bile acids and susceptibility to bile acid-induced intestinal barrier damage. In epithelial cell monolayers, Lactobacillus spp. mitigated bile acid-induced permeability, showing strain-dependent protective effects. In vivo, L. plantarum, either alone or within a Lactobacillus spp consortium, facilitated growth in protein-deficient mice, an effect attenuated by Giardia, despite not inhibiting Lactobacillus colonization. These results highlight Giardia’s potential role as a disruptor of probiotic functional activity, underscoring the imperative for further research into the complex interactions between parasites and bacteria under conditions of nutritional deficiency.

Evaluating effect of aeration and sand addition on soil physicochemical properties and soil total petroleum hydrocarbon bioremediation efficiency by indigenous isolated bacteria

In line with different investigations for bioremediation of oil-polluted soil at the global scale, the present study designed new insight about qualified physicochemical properties along with bioremediation of oil-polluted soil. The principle of presented bioremediation technique of oil-polluted soil in the present study was a combination of physical and biological procedure for the remediation of oil-polluted soils. The study investigates the impact of aeration and sand addition on bioremediation efficiency of two types oil-polluted soil with different ages of oil pollution. The experiment involves isolating and identifying indigenous bacteria, preparing the inoculum, and inoculating it into oil-contaminated soils. The physical and chemical properties of the soil samples were analyzed, and total petroleum hydrocarbon (TPH) levels were assessed before and after the bioremediation period followed by sand addition and air-forced soil aeration. The achieved results from laboratory-scale investigation show about 80% in soil TPH decreasing followed by sand addition and aeration for two types of fresh and aged polluted soils, while TPH decreasing in soil without sand addition and aeration was about 48% from studied soils. The findings of the present study can be utilized as parametric investigation in designing bioreactors of oil-polluted soil bioremediation purposes.

Bioremediation of metal cyanide complexes from electroplating wastewater for long-term application using Agrobacterium tumefaciens SUTS 1 and Pseudomonas monteilii SUTS 2.

The purpose of this study was to investigate the optimum conditions, including aerobic and anoxic conditions, for operating a long-term bioreactor system to decrease the toxicity of industrial electroplating wastewater effluents containing metal cyanide using Agrobacterium tumefaciens SUTS 1 and Pseudomonas monteilii SUTS 2. The initial results revealed that bacteria performed better under aerobic conditions than under anoxic conditions. An aerobic bioreactor system was subsequently set up in a long-term study lasting 30days under optimum operating conditions. Both mixed-culture bacteria and indigenous bacteria promoted the high-efficiency treatment of cyanide and metals in the first 7days of the study. When the system had high removal rates, cyanide removal was greater than that of zinc, copper, nickel, and chromium (CN- > Zn > Cu > Ni > Cr), with removal efficiencies of 96.67%, 93.93%, 74.17%, 63.43%, and 44.65%, respectively, with residual concentrations of 0.15 ± 0.01, 0.24 ± 0.005, 0.03 ± 0.002, 18.41 ± 0.06 and 14.26 ± 0.15mg/L, respectively. The cell concentration in the bioreactor increased to approximately 107CFU/mL over 30days from initial cell concentrations of 6.15 × 105 CFU/mL and 1.05 × 103CFU/mL for the mixed culture and indigenous inoculation, respectively. These results implied that the bacteria were resistant to heavy metal toxicity. The addition of an appropriate carbon source with sufficient aeration to a bioreactor resulted in increased cyanide degradation.

Comparison and mechanism study on solidification of loose pisha sandstone by indigenous bacteria and sporosarcina pasteurii

Pisha sandstone is a kind of sandstone which is easy to collapse by water in Shanxi, Shaanxi and Inner Mongolia of China, and suffers from hydraulic erosion all the year round. In recent years, some scholars have used microbial induced calcium carbonate precipitation (MICP) technology to solidify Pisha sandstone to improve the water erosion resistance of Pisha sandstone. However, for the climate environment with low average temperature in Pisha sandstone area, the commonly used Sporosarcina pasteurii are not well adapted. The purpose of this study is to use the indigenous strainsto solidify the loose Pisha sandstone, and to compare the growth adaptability, mechanical properties and water erosion resistance of the solidified layer with Sarcina pasteurii at different temperatures, and to explore the mechanism of different temperatures and strains affecting the microbial solidification of Pisha sandstone from the micro scale. At the same time, a mixed bacterial liquid solidification test was also set up. The results showed that the solidified thickness of indigenous strains was 4.65 % higher than that of Sporosarcina pasteurii, and the thickness and strength of mixed strains were increased by 19.57 % and 36.62 %, respectively. The growth and solidification effect of indigenous strains were less affected by low temperature. Compared with Sporosarcina pasteurii, at low temperature, the bacterial concentration decrease of indigenous strains was reduced by 26.13 %, the thickness loss of solidified layer was reduced by 13.04 %, and the strength loss of solidified layer was reduced by 13.39 %. The effect of low temperature on the growth of bacteria is mainly reflected in affecting the maximum concentration of bacteria and the growth rate. The effect on MICP mainly reflected in affecting the life activities of bacteria and the crystal form and morphology of calcium carbonate. The research results provide a theoretical basis for the MICP technology application of indigenous strains and multi-strains in Pisha sandstone area soil reinforcement and solidification slope.

Identification of Catabolic Genes Involved in the Degradation of Used Engine Lubricant- Contaminated Soil by Lysinibacillus odysseyi and Bacillus sp. (in: firmicutes)

This study aimed to identify selected catabolic genes in two indigenous hydrocarbon-degrading bacteria involved in the biodegradation of used engine lubricant-contaminated soil. Used engine lubricant-contaminated and uncontaminated soil samples were collected, and their physicochemical characteristics were evaluated. The results indicated that water holding capacity, potassium, nitrogen, and phosphate were higher in used lubricant-uncontaminated soil than in used lubricant-contaminated soil but vice versa for pH, total carbon, cation exchange, organic carbon, nickel, and lead levels. Culturable heterotrophic and hydrocarbon-utilizing bacterial counts were carried out on both Nutrient agar and Mineral Salt Medium (MSM) amended with used engine lubricant. The counts for heterotrophic bacteria (3.8×108±0.21 cfu/gm) were higher than that of hydrocarbon-utilizing bacteria (2.2×108±0.15 cfu/gm). All isolated bacteria (16) underwent screening for hydrocarbon degradation potential. Lysinibacillus odysseyi and Bacillus sp (in: firmicutes) emerged as the best oil degraders. Further screening of the chromosomal and plasmid DNA of the two bacteria was done to determine the presence and location of some selected catabolic genes (NidA, AlkB, NahH, NahAC, and Alma). The presence of Alkane monooxygenase (AlkB) and Naphthalene dioxygenase (NahAC) genes was confirmed in both isolates, while Pyrene dioxygenase (NidA) was confirmed in Bacillus sp. (in: firmicutes) only. The location of AlkB was confirmed to be both plasmid and chromosome, while NidA and NahAC genes were confirmed to be the plasmid. In conclusion, the soil contaminated with used engine lubricant contained indigenous bacteria, Lysinibacillus odysseyi, and Bacillus sp. (in: firmicutes), the two bacteria with the highest degradation potential, contained catabolic genes, monooxygenase (AlkB), NidA, and dioxygenase (NahAC). Therefore, they are effectively used as engine lubricant degraders, and the possibility of horizontal gene transfer can be used in important industrial applications and is recommended for the bioremediation of petroleum compounds.

Enhancing Compressive Strength of Cement by Indigenous Individual and Co-Culture Bacillus Bacteria

Using a Taguchi experimental design, this research focuses on utilizing indigenous bacteria from the Danube River to enhance the self-healing capabilities and structural integrity of cementitious materials. Bacillus licheniformis and Bacillus muralis were used as individual bacterium or in co-culture, with a concentration of 8 logs CFU, while the humidity variation involved testing wet and wet–dry conditions. Additionally, artificial neural network (ANN) modeling of the compressive strength of cement samples results in improvements in compressive strength, particularly under wet–dry conditions. By inducing targeted bacterial activity, the formation of calcium carbonate precipitates was initiated, which effectively sealed formed cracks, thus restoring and even enhancing the material’s strength. In addition to short-term improvements, this study also evaluates long-term improvements, with compressive strength measured over periods extending to 180 days. The results demonstrate sustained self-healing capabilities and strength improvements under varied environmental conditions, emphasizing the potential for long-term application in real-world infrastructure. This study also explores the role of environmental conditions, such as wet and wet–dry cycles, in optimizing the self-healing process, revealing that cyclic exposure conditions further improve the efficiency of strength recovery. The findings suggest that autochthonous bacterial co-cultures can be a viable solution for enhancing the durability and lifespan of concrete structures. This research provides a foundation for further exploration into bio-based self-healing mechanisms and their practical applications in the concrete industry.

Detoxification of ars genotypes by arsenite-oxidizing bacteria through arsenic biotransformation.

The detoxification process of transforming arsenite (As(III)) to arsenate (As(V)) through bacterial oxidation presents a potent approach for bioremediation of arsenic-polluted soils in abandoned mines. In this study, twelve indigenous arsenic-oxidizing bacteria (AOB) were isolated from arsenic-contaminated soils. Among these, Paenibacillus xylanexedens EBC-SK As2 (MF928871) and Ochrobactrum anthropi EBC-SK As11 (MF928880) were identified as the most effective arsenic-oxidizing isolates. Evaluations for bacterial arsenic resistance demonstrated that P. xylanexedens EBC-SK As2 (MF928871) could resist As(III) up to 40 mM, while O. anthropi EBC-SK As11 (MF928880) could resist As(III) up to 25 mM. From these bacterial strains, genotypes of arsenic resistance system (ars) were detected, encompassing ars leader genes (arsR and arsD), membrane genes (arsB and arsJ), and aox genes known to be crucial for arsenic detoxification. These ars genotypes in the isolated AOBs might play an instrumental role in arsenic-contaminated soils with potential to reduce arsenic contamination.

Metabolic route and bacterial community changes induced by inactivation of indigenous bacteria in dark fermentation

Biohydrogen production by dark fermentation (DF) can be achieved using only indigenous bacteria from substrates or by adding an external inoculum. This study aims to provide new insights on the role of indigenous bacteria in DF process operation by investigating DF performances and microbiological aspects. DF tests are performed with only indigenous bacteria, with indigenous and exogenous bacteria and with only exogenous bacteria by inactivating indigenous bacteria by gamma irradiation. Sorghum irradiation reduces DF performances for both hydrogen (17.8 ± 12.8 versus 45.2 ± 1.7 mLH2/gVSadded) and total metabolite (0.22 ± 0.01 versus 0.30 ± 0.01 gCOD/gVSadded) yields. In contrast, no difference is observed with the organic fraction of municipal solid waste, suggesting a distinct role of indigenous bacteria for both substrates. Indigenous bacteria inactivation strongly modifies the metabolic routes and final bacterial community composition. This study proves the key role of indigenous bacteria in influencing metabolite production and bacterial composition.

Biosurfactant production by Bacillus cereus GX7 utilizing organic waste and its application in the remediation of hydrocarbon-contaminated environments.

The use of biosurfactants represents a promising technology for remediating hydrocarbon pollution in the environment. This study evaluated a highly effective biosurfactant strain-Bacillus cereus GX7's ability to produce biosurfactants from industrial and agriculture organic wastes. Bacillus cereus GX7 showed poor utilization capacity for oil soluble organic waste but effectively utilized of water- soluble organic wastes such as starch hydrolysate and wheat bran juice as carbon sources to enhance biosurfactant production. This led to significant improvements in surface tension and emulsification index. Corn steep liquor was also effective as a nitrogen source for Bacillus cereus GX7 in biosurfactant production. The biosurfactants produced by strain Bacillus cereus GX7 demonstrated a remediation effect on oily beach sand, but are slightly inferior to chemical surfactants. Inoculation with Bacillus cereus GX7 (70.36%) or its fermentation solution (94.38%) effectively enhanced the degradation efficiency of diesel oil in polluted seawater, surpassing that of indigenous degrading bacteria treatments (57.62%). Moreover, inoculation with Bacillus cereus GX7's fermentation solution notably improved the community structure by increasing the abundance of functional bacteria such as Pseudomonas and Stenotrophomonas in seawater. These findings suggest that the Bacillus cereus GX7 as a promising candidate for bioremediation of petroleum hydrocarbons.

Use of Indigenous Lactic Acid Bacteria for Industrial Fermented Sausage Production: Microbiological, Chemico-Physical and Sensory Features and Biogenic Amine Content

The use of starter cultures in the meat industry is common, even if the number of available commercial cultures is limited, inducing product standardisation and microbial diversity reduction. On the other hand, some artisanal products relying on spontaneous fermentation can represent a source of isolation of new interesting strains. In this work, four LAB strains derived from Mediterranean spontaneously fermented sausages were tested as new starter cultures for the industrial production of fermented sausages, in comparison to a commercial starter culture. The products obtained were analysed for physico-chemical parameters, microbiota, biogenic amines and aroma profile. A consumer test was also performed to evaluate product acceptability. The strains induced different acidification kinetics. LAB counts showed high persistence when Latilactobacillus curvatus HNS55 was used as the starter culture, while the addition of Companilactobacillus alimentarius CB22 resulted in a high concentration of enterococci (6 log CFU/g), 2 log higher than in other samples. Tyramine was detected at concentrations of 150–200 mg/kg, except for in the sample produced with Lactiplantibacillus plantarum BPF2 (60 mg/kg). Differences were observed in the aroma profile, with a high amount of 2-butanone found in the samples obtained with Comp. alimentarius CB22. These latter sausages also showed the lowest score in terms of acceptability. This study allowed us to select new LAB strains for fermented sausage starter cultures, increasing the product diversification.

Characterization of Cr(VI)-reducing indigenous bacteria from a long-term tannery waste-contaminated soil

This paper investigated the Cr (VI) detoxification potential of 4 novel bacterial strains isolated from a long-term tannery waste-contaminated soil. Molecular techniques were used to identify the bacterial strains using 16 S rDNA gene sequencing. The Cr (VI) detoxification capacity of the bacteria was determined by 1,5-diphenylcarbazide (DPC) methods. The identified bacterial strains were Bacillus subterraneus MMKT-10, Klebsiella quasivariicola MMKT-15, Acinetobacter seohaensis MMKT-19, and Staphylococcus saprophyticus MMKT-25. All the strains showed maximum tolerance concentration (MTC) of Cr (VI) up to 4000 mg/L. However, in terms of Cr(VI) reduction rate, K. quasivariicola can be considered the most efficient, reducing the two preliminary Cr(VI) concentrations (10 and 20 mg/L) at 15 and 18 h, respectively, while the rest of the strains needed 30 h to reduce the same concentrations from the culture medium. The favorable temperature for Cr(VI) detoxification ranged from 30–40 °C. However, 100 % Cr (VI) reduction was achieved by all the strains at 35 0C. Interestingly, all the bacterial strains reduced a significant amount of Cr (VI) at 50 0C, indicative of their thermotolerant nature. The ideal pH for Cr (VI) reduction was 7 for B. subterraneus MMKT-10 and K. quasivariicola MMKT-15, whereas it was 8 for Acinetobacter seohaensis MMKT-19 and Staphylococcus saprophyticus MMKT-25. The indigenous bacterial strains isolated in this study could be one of the promising candidates for the detoxification of Cr (VI) contaminated sites.

Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru.

Pollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new bacterial strains from underexplored habitats could improve remediation efforts at contaminated sites. This study aimed to evaluate the hydrocarbon biodegradation capacity of bacteria isolated from agricultural soils in Huamachuco, Peru. Soil samples from Oca crops were collected and bacteria were isolated. Biodegradation assays were conducted using diesel as the sole carbon source in the Bushnell Haas Mineral medium. Molecular characterization of the 16S rRNA gene identified four strains. Diesel biodegradation assays at 1% concentration were performed under agitation conditions at 150 rpm and 30 °C, and monitored on day 10 by measuring cellular biomass (OD600), with hydrocarbons analyzed by gas chromatography. The results showed Pseudomonas protegens (PROM2) achieved the highest efficiency in removing total hydrocarbons (91.5 ± 0.7%). Additionally, Pseudomonas citri PROM3 and Acinetobacter guillouiae ClyRoM5 also demonstrated high capacity in removing several individual hydrocarbons. Indigenous bacteria from uncontaminated agricultural soils present a high potential for hydrocarbon bioremediation, offering an ecological and effective solution for soil decontamination.

Enhanced indigenous bacteria-mediated bioremediation of aged petroleum-contaminated soil by Fenton pretreatment: Synergistic effect of soil environmental improvement and microbial community response

Due to the harsh soil environment and low microbial activity, the efficacy of indigenous bacteria-mediated bioremediation is often limited in aged petroleum-contaminated soils. In this study, Fenton pretreatment (H2O2: 200 ∼ 600 mmol/kg, H2O2: Fe2+ = 100: 1) was applied to improve the aged soil environment and rebuild the microbial community to enhance subsequent bioremediation. After pretreated by 400 mmol/kg H2O2 and 4 mmol/kg Fe2+ (T2), the removal efficiency of total petroleum hydrocarbons (TPHs) was elevated to 93.27 %, equivalent to 1.96-fold of the natural attenuation (CK). Additionally, the half-life of biodegradation was shorted to 45.57 d, representing a decrease to 0.29-fold that of CK. Correlation analysis revealed a radar plot comprising five indicators, including bacterial quantification (BQ), dehydrogenase (DHA), polyphenol oxidase (PPO), permeability coefficient (K), and bulk density coefficient (γ), all of which exhibited positive correlations with TPHs degradation. Compared to CK, Fenton pretreatment increased the richness and evenness of the microbial community during incubation, thus facilitating TPHs biodegradation. Moreover, Fenton pretreatment accelerated the shift of the dominant genus in T2 from Pseudomonas (0.599) to Pseudomonas (0.258), Nocardioides (0.109), Dietzia (0.157), and Stenotrophomonas (0.120) throughout the incubation. Based on the analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG), the metabolism and enzymatic functional genes related to the biodegradation of petroleum hydrocarbons in T2 were enhanced during incubation, accounting for the high removal efficiency of TPHs. These findings demonstrated that moderate Fenton pretreatment was an effective approach to stimulate indigenous bacteria for the enhanced biodegradation of aged petroleum-contaminated soil.

Identification of novel broad host-range promoter sequences functional in diverse Pseudomonadota by a promoter-trap approach.

Finding novel promoter sequences is a cornerstone of synthetic biology. To contribute to the expanding catalog of biological parts, we employed a promoter-trap approach to identify novel sequences within an Antarctic microbial community that act as broad host-range promoters functional in diverse Pseudomonadota. Using Pseudomonas putida KT2440 as host, we generated a library comprising approximately 2,000 clones resulting in the identification of thirteen functional promoter sequences, thereby expanding the genetic toolkit available for this chassis. Some of the discovered promoter sequences prove to be broad host-range as they drove gene expression not only in P. putida KT2440 but also in Escherichia coli DH5α, Cupriavidus taiwanensis R1T, Paraburkholderia phymatum STM 815T, Ensifer meliloti 1021, and an indigenous Antarctic bacterium, Pseudomonas sp. UYIF39. Our findings enrich the existing catalog of biological parts, offering a repertoire of broad host-range promoter sequences that exhibit functionality across diverse members of the phylum Pseudomonadota, proving Antarctic microbial community as a valuable resource for prospecting new biological parts for synthetic biology.

Dietary supplementation of Roseovarius litoreus improves survival and immune defense of the swimming crab Portunus trituberculatus

The aquaculture of the swimming crab Portunus trituberculatus is challenged by vibriosis, and Rhodobacteraceae strains play a crucial role in maintaining gut health and enhancing the immunity of crustaceans. In this study, we investigated the effects of dietary supplementation of Roseovarius litoreus, a member of Rhodobacteraceae, on the survival and immune defense of the swimming crab. The results showed that 60-day dietary supplementation of R. litoreus resulted in a marked increase in the mRNA expression of gut ZO-1. Meanwhile, R. litoreus increased zero-radius operational taxonomic unit (ZOTU) richness and changed the gut bacterial community, which was manifested by the enriched Carboxylicivirga and Roseovarius and depleted Photobacterium and Vibrio, thereby upregulating functional potentials of the gut bacterial community in the immune system and biosynthesis of other secondary metabolites. After a 72-h V. parahaemolyticus challenge, R. litoreus-supplemented crabs had an increased survival rate, reduced histological damage of hepatopancreas and gut, and improved expressions of immune-related genes and lysozyme activity. Moreover, R. litoreus-supplemented crab gut still harbored more Carboxylicivirga and Roseovarius but had fewer Vibrio gene copies than control crabs, which led to similar changes in functional potentials. Notably, all ZOTUs that were positively correlated with immune-related genes and lysozyme activity belonged to Bacteroidetes, Bacteroidales, or Carboxylicivirga. Taken together, we highlight the probiotic role of R. litoreus in the survival and immune defense of the swimming crab. These findings may contribute to the development of indigenous bacteria of the swimming crab as potential probiotics for the control of vibriosis.

Co-cultivation of microalgae and bacteria for optimal bioenergy feedstock production in wastewater by using response surface methodology

This work uses response surface methodology (RSM) to study the co-cultivation of symbiotic indigenous wastewater microalgae and bacteria under different conditions (inoculum ratio of bacteria to microalgae, CO2, light intensity, and harvest time) for optimal bioenergy feedstock production. The findings of this study demonstrate that the symbiotic microalgae-bacteria culture not only increases total microalgal biomass and lipid productivity, but also enlarges microalgal cell size and stimulates lipid accumulation. Meanwhile, inoculum ratio of bacteria to microalgae, light intensity, CO2, and harvest time significantly affect biomass and lipid productivity. CO2 concentration and harvest time have significant interactive effect on lipid productivity. The response of microalgal biomass and lipid productivity varies significantly from 2.1 × 105 to 1.9 × 107 cells/mL and 2.8 × 102 to 3.7 × 1012 Total Fluorescent Units/mL respectively. Conditions for optimum biomass and oil accumulation are 100% of inoculation ratio (bacteria/microalgae), 3.6% of CO2 (v/v), 205.8 µmol/m2/s of light intensity, and 10.6 days of harvest time. This work provides a systematic methodology with RSM to explore the benefits of symbiotic microalgae-bacteria culture, and to optimize various cultivation parameters within complex wastewater environments for practical applications of integrated wastewater-microalgae systems for cost-efficient bioenergy production.

Hormesis of black soldier fly larva: Influence and interactions in livestock manure recycling

Black soldier fly larvae (BSFL) are considered important organisms, utilized as tools to transform waste including manure into valuable products. The growth and cultivation of BSFL are influenced by various factors, such as the presence of toxic substances in the feed and parasites. These factors play a crucial role in hormesis, and contributing to regulate these contaminants hermetic doses to get sustainable byproducts. This review aims to understand the effects on BSFL growth and activities in the presence of compounds like organic and inorganic pollutants. It also assesses the impact of microbes on BSFL growth and explores the bioaccumulation of pharmaceutical compounds, specifically focusing on heavy metals, pesticides, pharmaceuticals, indigenous bacteria, insects, and nematodes. The review concludes by addressing knowledge gaps, proposing future biorefineries, and offering recommendations for further research.

Identification of Indigenous Bacterial Strains from Thai Agricultural Fields for Potential Bioremediation of Carbofuran

This study explored the bioremediation potential of indigenous soil bacteria for carbofuran-contaminated agricultural areas. Twenty soil samples from regions with a history of pesticide use in Pathum Thani and Nakhon Pathom provinces, Thailand, were collected. Five bacterial strains capable of degrading carbofuran were isolated and subjected to cell morphology and biochemical analyses. Phylogenetic analysis of the 16S rRNA gene sequences identified these strains as related to Pseudomonas and Stenotrophomonas species. High-Performance Liquid Chromatography (HPLC) analysis confirmed the complete degradation of carbofuran within 3 days, reducing its concentration from 0.05 mg/mL to below detectable limits. These bacteria could use carbofuran as their sole carbon source, demonstrating their potential for bioremediation of contaminated soils. Given their resilience and ability to thrive in natural environmental conditions, these indigenous strains are well-suited for in-situ degradation of pollutants. The findings indicate that these isolated soil bacteria present a promising method for reducing the environmental risks associated with carbofuran contamination.

Assessing the effectiveness of indigenous phosphate-solubilizing bacteria in mitigating phosphorus fixation in acid soils.

The online version contains supplementary material available at 10.1007/s13205-024-04042-2.