This research leads to the analysis of quality parameters and chemical profiles of Artemisia maritima essential oils (EOs) extracted from four different wild areas viz: Darcha, Jispa, Gemur and Tandi of Lahaul valley of Indian western Himalaya in comparison to a market sample. The EOs content was found higher in Darcha (0.54%) and Jispa (0.48%). The quality parameters such as color, odor, density, refractive index and optical rotations were found comparable with the market sample. Further, volatile chemo‐profile of EOs was generated using GC/GC‐MS. A total of 34 metabolites were identified in five samples (n=3) that account around 87.3‐95.1% of the total oils. These EOs majorly contained monoterpene hydrocarbons (3.0‐68.5%) and oxygenated monoterpenes (26.6‐85.0%) with β‐myrcene (9.8‐20.6%), α‐terpinene (0.7‐17.3%), β‐phellandrene (1.5‐22.4%), 1,8‐cineole (3.0‐50.8%), terpinen‐4‐ol (2.5‐7.2%), ascaridole (11.5%), bornyl acetate (5.1‐14.2%), trans‐sabinyl acetate (0.8‐49.6) and trans‐ascaridole (7.9%). Moreover, cytotoxicity study of EOs against CaCo2 and HT‐29 human colon carcinoma cell lines unveiled significant anticancer potential of A. maritima. AMD (82.87±0.39%) AMG (61.17±1.45%) and AMM (73.37±2.1%) showed comparable activity at 600 µg/ml (72 hr) in comparisons to vinblastine (20 µM). A. maritima is less explored as compared to other species and could be a new potential source for EOs and anticancer candidate.