Indian Hemp Research Articles

Peripheral Ischemia and Necrosis Revealing Buerger's Disease in an Internal Medicine Department

Buerger's disease is a systemic vasculitis strongly correlated with tobacco use. It entails a heavy socio-occupational impact. We report the case of a 26-year-old patient with ischemia of the lower limbs revealing Buerger’s disease. He is known smoker with passive exposure to Indian hemp. He was seen for ischemic foot pain that had been progressing for a year. Involvement began in the left big toe. Subsequently, hyperalgesic necrotic lesions were observed on the 3rd, 4th and 5th toes of the foot. Biological investigations revealed an inflammatory syndrome with normocytic anemia and increased C-reactive protein. Retroviral, syphilitic, Hepatitis B and C viruses and SARS-CoV-2 serologies were negative. Antinuclear antibodies were initially borderline at 100 IU with speckled fluorescence, then negative on control. Neutrophil cytoplasmic antibodies and antiphospholipid antibodies were negative. Investigation of thrombophilia was non-contributory, notably factor V mutation testing, antithrombin III assay, proteins C and S and fibrinogen. An arterial ultrasound revealed extensive arterial thrombosis with thickening of the femoral arterial vessel walls. Thromboangiitis obliterans was confirmed and the patient was put on corticosteroids and adjuvant therapy. Surgical treatment was performed 4 months later. Buerger's disease is a serious vascular disorder which must be diagnosed very early in order to prevent complications. Early smoking cessation leads to remission in the early phase.

Untangling Indian Hemp: Identifying Woodlands Bast Fibers in Collections

ABSTRACT Many textiles produced by Native American Woodlands cultures are made from bast (phloem) fibers from North American plants. While bast fibers are easy to identify with microscopy, determining the exact species has long been a challenge. Historically, bast fibers from Native American communities have been referred to as “Indian hemp,” a confusing term at best. This project undertaken as part of an Andrew W. Mellon Fellowship at the National Museum of the American Indian (NMAI) focused on a combined approach by working with both community members and scientists to provide a cultural background for these important fibers as well as useful diagnostic tools for identification. Both macroscopic and microscopic characteristics were examined including color, feel, microfibrillar orientation, crystal species, and cross section analysis. A physical and digital reference collection was created for comparison. Eight fiber samples taken from six historic objects in NMAI’s collection were then examined to test effectiveness of bast fiber identification protocol. Results from this study confirm there continues to be limited success when identifying the exact bast fiber species in historic artifacts because of small sample size and aging characteristics.

Characterization of Indian hemp (Canabinus sativa L.) fibre and study for potentiality in textile application

Characterization of Indian hemp (Canabinus sativa L.) fibre and study for potentiality in textile application

Judicial Interpretations, Findings and Pronouncements of the Nigerian Apex Court: A Review of Mohammed Vs Frn

Purpose: The objective of this review was to challenge the apex court's decision, encompassing its findings, judicial interpretations, and pronouncements (DFIP). A notable issue arises from the discovery that the National Assembly (NA) mistakenly granted 'exclusive' criminal jurisdiction to the Federal High Court (FHC) regarding Indian hemp offenses. Consequently, this study critically examined the DFIP in depth.
 Methodology: This work adopts the doctrinal method of research, which involves the use of primary and secondary sources of law. This study relied on key primary sources, including the constitution of the Federal Republic of Nigeria, Acts of the National Assembly, case laws, and Decrees. Supplementary information was derived from secondary sources such as books and articles. This review extensively employed primary and secondary legal sources, focusing on statutory interpretation rules, the hierarchy of norms, particularly in relation to the principle of inconsistency, and the unalterable nature of jurisdiction prescribed by the Constitution of the Federal Republic of Nigeria (CFRN). These criteria were systematically applied to evaluate the Decision, Findings, Interpretations, and Pronouncements (DFIP) of the apex court.
 Findings: The apex court's determination that Section 8 of the Indian Hemp Act (IHA) was implicitly repealed by Section 26 of the National Drug Law Enforcement Agency (NDLEA) Act is inaccurate. Similarly, the assertion that the National Assembly (NA) granted 'exclusive' criminal jurisdiction to the Federal High Court (FHC) is flawed, as Section 251(3) of the Constitution of the Federal Republic of Nigeria (CFRN) actually confers concurrent criminal jurisdiction on the FHC. 
 The finding of the apex court that Magistrate Courts (MC), being a creation of state legislation, cannot try Indian hemp offences is erroneous. The apex court's assertion that Indian hemp is both a 'drug' and a 'narcotic drug' is refuted as erroneous in the course of this review.
 Recommendations: Statutory intervention in the manner of amendment of Sections 251(1)(s) and 251(3) is required if the decision of the apex court is to represent the position of the law in Nigeria.

Settling ‘Dagga’? Shifting Frontiers of Cannabis Knowledge and Governance in South Africa

After the South African War (1899–1902), state-makers’ efforts to control ‘dagga’ was controversial on several fronts. But ‘dagga’ also proved a moving target for official classification. Was it a species of Leonotis, common around the countryside? Was it ‘Indian hemp’, understood by some as a habit-forming drug that debilitated wage workers and caused insanity? This paper traces dagga as a multiple object and problematic of governance in South Africa during the years before the formation of the Union of South Africa and into the early decades of the Union period. A focus on three contested boundaries of top-down knowledge-making and policy – botanical taxonomies; colonial geographies; and political-economy – demonstrates dagga’s shifting ontologies across time and space. Together, these empirical snapshots combine as a case study, revealing how the legal reification of a substance as a ‘drug’ involved political processes that were local, dispersed and unresolved. We show how uncertainties and ambivalences about cannabis long remained productive for different brokers and gatekeepers who navigated the frontiers of competing interests. This history is important for understanding the changing politics of cannabis in South Africa, as it became legible for regulation as a ‘dangerous drug’ during the first half of the 20th century.

A Study of the Prevalence of Substance Consumption Among In-School and Out-of-School Youths in ETI OSA Local Government Area of the Lagos Metropolis

This study quantifies the prevalence of substance use among youth in the Eti-Osa Local Government Area of the Lagos Metropolitan Region. This research examines both in and out-of-school youths in a cross-sectional manner. Using a multistage sampling method, four hundred respondents were chosen as the sample population from the Eti-Osa LGA population. Data were gathered using a quantitative, then analysed using SPSS. The study indicated that the group under observation had a high knowledge of problematic substance use (93.5%). That substance use was common and most prevalent in male youth. Indian Hemp and Tramadol were the two most complex substances most frequently consumed. Nonetheless, codeine was more frequently ingested by the individuals under study. These drugs are pretty widely available and reasonably priced. Friends (they inspired 88% of users), followed by social media (41%), movies and entertainment (48%), and other sources (48%). The use of drugs is increasing and could soon become out of control. The government must provide the youths with proper management and control through education, campaigns, and counselling.

English

In the last decade, there have been different mental health challenges among students due to academic stress, especially during examinations. This has led to the need to explore the relationship between psychoactive ingredients, behavioral responses and academic stress in relation to the use of hair creams. The objectives of the study were to identify hair creams containing psychoactive ingredients in use by female students, assess behavioral responses manifested by female students due to use of these hair creams and compare behavioral responses due to the use of these hair creams across the different departments as it relates to academic workload. The study was a cross-sectional survey of four hundred female students (100 to 500 levels) of the Obafemi Awolowo University Ile-Ife, in the Faculties of Pharmacy, Basic Medical Sciences, Administration and Social Sciences. The sample size was determined and randomly obtained from the population. A semi-structured questionnaire which was used as a data collection instrument was pretested. The data was subsequently organized with descriptive statistics like frequencies, percentages and analyzed with inferential statistics like Chi Square, Kruskal-Wallis tests and Mann Whitney test for post-hoc determinations. Twelve percent of the respondents confirmed using hair creams containing psychoactive ingredients. The hair products that contain psychoactive ingredients were Morgan hair cream (4.2%), Dax (2.8%), Mega growth (2.8%), Kuza Indian Hemp (1.4%) and Bergamot (0.8%). The behavioral responses (Anxiety (p=0.217), Mood disorders (p=0.14) and Depression (p=0.234)), were not significantly manifested due to psychoactive ingredients of hair creams. Also, there was no significant relationship existing between academic workload and behavioral responses from the use of hair creams (Anxiety (p=0.079), Mood disorders (p=0.141) and Depression (p=0.169)). The study concluded that only 12% of hair creams contain psychoactive ingredients and these hair creams are Morgan Dax, Mega growth, Kuza Indian Hemp and Bergamont hair creams. Data gathered indicated that there was insufficient evidence linking female students' usage of hair products containing psychoactive chemicals with their behavioral responses, and also, that academic workload did not have a role in the behavioral responses that were being displayed. Key words: Psychoactive ingredients, behavioral responses, academic stress, hair creams.

Introduction to Opioids: A Review

Opioids are psychoactive chemical substances that have been known to reduce feelings of pain. They are a class of drugs that have been implicated in depressing the central nervous system and causing several physical and psychological reactions including numbness, inducing sleep, hyperactivity, drowsiness, mental confusion, nausea, euphoria as well as constipation. The commonest examples of opioids are tramadol, heroin, morphine, codeine, etc. The main reason for Opioid use is for therapeutic purposes. However, the use of Opioids has also been widely implicated in increasing energy and libido. It is also used as a coping mechanism against pressure, the impact of post-traumatic stress, poverty, crime, etc. The indiscriminate use of opioids is usually associated with overdose, addiction and withdrawal. This study focuses on the incidence of opioid use in Nigeria that has become an epidemic in all regions of the country. In addition to being an active component of cough syrups, Codeine and tramadol which are the predominant types of opioids in Nigeria, have been reported to be a leading cause of health implications and fatality amongst Nigerians, cutting across religion, gender, age, social and educational backgrounds. Due to their availability, ease of accessibility, relative affordability, and the euphoric sensation they cause, Codeine and Tramadol have been tremendously used indiscriminately. There are recorded incidents of fatal overdose and adverse interactions between opioids and other drug classes such as Indian hemp. The addictive ability and the resultant antisocial behaviour, fatality and potential health implication poses Opioid use as a threat in the society. This menace has therefore incited the government to put measures in place to enforce the reduction in Opioid use.

‘You want to chill with the big boys’: Proverbs 21:4–7 and drug trafficking in Nigeria

Although progress has been made in the study of Proverbs 21:4–7, existing literature is yet to shift focus on the relevance of the pericope in examining the different aspects of drug trafficking by Nigerians. There is no month that passes without the news of a Nigerian caught in a national or international airport for one form of drug trafficking or another by the National Drug Law Enforcement Agency (NDLEA). These drugs include cocaine, tramadol and Indian hemp, among others. This has brought disgrace to those caught in the act, their families as well as Nigerian society. Those who were supposed to protect and fight against these drug traffickers have also involved themselves, as pastors and policemen have also been caught trafficking drugs. Proverbs 21:4–7 is a storehouse of moral instruction and has always been regarded as containing the concentrated deposit of ancient Israelite morality. It was written to sharpen an individual’s ability not to be crafty or cunning but to transform a person of evil devices into a person of discretion, turning craftiness into prudence. This study used literary analysis as its research method. There is a close relationship between Proverbs 21:4–7 and the activities of drug traffickers in Nigeria. The themes embedded in the pericope, such as contentment, righteousness, diligence, self-control and patience, are believed to speak anew to the increasing activities of drug traffickers in Nigeria.Intradisciplinary and/or interdisciplinary implications: Drug trafficking by Nigerians, nationally and internationally, has increased social crimes and Proverbs 21:4–7 provides new approach for solving this anomaly.

Analysis and Identification of Suspected Substance in the Trial of Drug Offences in Nigeria: A Review of Oyem v FRN

Purpose: The purpose of the review was to show that the affirmation of the appellant’s conviction in the case of Oyem v FRN by the Nigerian apex court was erroneous. To achieve the purpose, it is expedient to unravel the various errors committed by the apex court as a result of its failure to adhere to the law and to observe the peculiarities in the procedure for the trial of drug offences.
 Methodology: This work adopts the doctrinal method of research which involves the use of primary and secondary sources of law. The primary sources used in this work were legislation (Acts of the National Assembly), Case laws and Decrees while the secondary sources were books and article. The review of Oyem’s case in substance reveals that the apex court affirmed the conviction of the appellant on the assumption that, (1) the result of the preliminary (color) test conducted by the exhibit keeper at the NDLEA Command, using UN Narcotic Identification Testing Kit was the legal proof of the suspected substance as Indian hemp, instead of a chemist’s report on the mandatory confirmatory laboratory test by an analyst and, (2) the appellant’s confessional statement and guilty plea served as alternative proof that the suspected substance was Indian hemp even when the trial court did not carry out the test of veracity on the confessional statement.
 Findings: The two (2) assumptions by the apex court are the main findings in this work. Furthermore, even when the appellant was charged, tried and convicted for an offence constituted by expert evidence, no expert gave evidence at the trial, at least not on record. This work is of the view that extra-judicial confession made prior to analysis of a suspected substance cannot serve as proof of the nature of the substance, that is, Indian hemp. Moreover, the Nigerian Evidence Law having provided for the method for the analysis and identification of suspected substance in the trial of drug offences no other method can be used under the law.

Overlooked Influence of Indian Hemp (Cannabis sativa) Cultivation on Soil Physicochemical Fertility of Humid Tropical Agroecosystems: Upland Soils

Overlooked Influence of Indian Hemp (Cannabis sativa) Cultivation on Soil Physicochemical Fertility of Humid Tropical Agroecosystems: Upland Soils

Antioxidant Activity and Profile of Phenolic Compounds in Selected Herbal Plants

Herbs are characterized by a high content of biologically active substances that positively affect human health. Phenolic compounds are one of the main bioactive compounds in these plants with highly beneficial properties (e.g., anti-carcinogenic, cardioprotective, immune system support and antibacterial). Therefore, this study aimed to determine the composition of free and bound phenolic compounds and antioxidant activity in 10 different herbs (bogbean leaves, sage leaves, whole Indian hemp, whole heartsease, whole horsetail, whole blessed thistle, whole thyme, chamomile flower, corn silk and pine buds). Phenolic compounds were analyzed using the HPLC-TOF-MS/MS method, total phenolic (TP) and flavonoid (TF) contents were measured using spectrophotometric methods, while antioxidant activity was determined by ABTS and DPPH assays. The highest content of free TP was found in thyme, while sage was characterized by the highest range of these compounds released from ester and glycosidic derivatives by hydrolysis. In turn, the highest values of TF in non-hydrolyzed extracts were found in Indian hemp. The highest values of TF, including bound phenols were observed in extracts obtained from sage leaves. In the analyzed herbs thirty-three phenolic compounds were found, constituting 16 phenolic acids, 9 flavonoids and eight stilbenes. Moreover, the analyzed compounds appeared in the bound form than in the free form. The antioxidant activity of herb extracts differed significantly across varieties (P < 0.05). The research showed that each tested herb possesses its own fingerprint of phenolic compounds and antioxidant properties.

Legal status of Cannabidiol

The plant Cannabis sativa L., is a dioecious species belonging to the familie Cannabaceae, natived to Central Asia, with a long history. The first data on the use of this plant date from 2500 BC [1]. In Europe, the plant began to be cultivated between the 14th and 15th centuries, during which time it played an important role in agriculture, helping economic growth. In the Western world, it was increasingly used in nineteenth-century medicine [2]. It was cultivated worldwide in the 18th and 19th centuries as a source of fiber, food and oil. The main ingredients of hemp oils are phytocannabinoids such as cannabidiol (CBD) and terpenoids. The main active compound in the plant is d-9-tetrahydrocannabinol (D-9-THC), which is largely responsible for the psychoactive effects that made Indian hemp famous. While d9-THC has returned to the attention of researchers, other constituents of cannabis, such as CBD, have been studied in recent years for its therapeutic uses [3].Hemp has gained substantial attention in recent years due to the fact that an increasing number of countries legalized Cannabis for medicinal and recreational use. Taking into account the current legislation, there are small differences in the amounts of THC allowed in hemp preparations, ranging from 0.05 to 0.6%. As a result, if the plant contains THC it has a high illicit use and interest, therefore its cultivation is prohibited by national laws [4]. Among the illicit cannabis preparations, we can mention the following: marijuana (a mixture of leaves, flowers and seeds of the hemp plant), hashish (obtained from unfertilized buds) and also oils that can be easily prepared [5].Globally, the use of cannabis-derived preparations in the medical field has a long history. In the twentieth century, consumption became limited until March 30, 1961, when the Cannabis plant and cannabinoids were classified in the Single UN Convention as non-medical substances. In recent years, however, patients' interest in the use of cannabinoids has increased [6].CBD, the main phytocannabinoid obtained from Cannabis sativa, brings new hope to patients suffering from a wide range of conditions: pain, inflammation, epilepsy, sleep disorders, multiple sclerosis, anorexia, schizophrenia, cancer.

OPULATION AT DANGER ZONE: ANALYSIS OF YOUTH CONSUMPTION OF PSYCHOTROPIC SUBSTANCES IN APAPA LOCAL GOVERNMENT, LAGOS STATE

Consumption of substances constitutes a health problem, especially among youth globally. Substances become a problem when they are consumed against professionals’ advice. Thus, this study analyses youth involvement in the consumption of psychotropic substances in Apapa Local Government, Lagos State. The study focused on the following objectives: to determine the predisposing factors to the harmful consumption of psychotropic substances; find out the types of psychotropic substances commonly consumed; examine the health problems associated with harmful consumption of psychotropic substances by the youths in Apapa Local Government Area of Lagos State. The Edwin Sutherland’s Differential Association theory was adopted as the theoretical framework for the subject matter. A qualitative narrative research design with the use of an in-depth interview guide was employed to gain insight and understanding about the experiences of thirty-two (32) psychotropic users, who were selected from across six wards of the study area through purposive and snowball sampling techniques. Findings revealed that lack of parental supervision; availability of drugs; and the need to gather extra energy to work for long hours, among others were largely responsible for harmful consumption of the substances. The various types of psychotropic substances consumed by youths in the study area include cannabis [popularly known as Indian hemp], alcohol, tramadol and codeine. The study further reveals that the youth involvement in harmful consumption of psychotropic substances had made them suffer health challenges, such as chronic cough, weight loss, tooth decay, bad breath, dry mouth and throat and headache. The study concludes that harmful consumption of psychotropic substances exposes youth to terminal illnesses and premature deaths. The study therefore recommends that treatment programs such as counselling, medication and other behavioural therapies should be readily available and be effective in attending to the multiple needs of addicted psychotropic users’ problems to mitigate their involvement in the consumption of the psychotropic substances.

THE USE OF PSYCHOACTIVE DRUGS AMONG STREET LADIES IN ABA METROPOLIS OF NIGERIA: AN ASSESSMENT OF PUBLIC HEALTH IMPLICATIONS

Introduction: The use of psychoactive drugs among ladies is engaged in on an alarming increase in the postmodern Nigerian society, as it remains one of the public health and social vices that affects the contemporary human community.&#x0D; Purpose: The objectives of this study were to identify the drugs mostly abused and to determine the risk behaviours they engage in when under the influence of psychoactive drugs.&#x0D; Methodology: This study adopted a cross-sectional approach involving the use of oral interview and a semi- structured questionnaire as the instrument of data collection. This involved 20 interviewees in simple random selection. The data collected were analyzed using a descriptive statistics and statistical test of significance and results presented in charts.&#x0D; Findings: Major findings in this study attest to the fact that the mostly abused psychoactive drugs are Cigarette, Indian hemp, tramadol and codeine, cocaine. The use of psychoactive drugs is precursory to mental health challenges, depression, and behavioural abnormalities among younger women. The risk behaviours engaged when under the influence of these psychoactive drugs are aggressiveness/ ready to fight, unrestricted sexual activities, desire for more drug use. Consequently, the study findings maintain that the perpetration of heinous crimes that are carried out in the society cannot be independent of the influence of drugs, since the abused psychoactive drugs alter the normal function of the brain thus affecting the mental health of the user.&#x0D; Contribution to theory, practice and policy: Therefore, public health education and promotion with a focus on dissuading the unrestricted circulation and abuse of psychoactive drugs, especially among Aba young ladies is perceived profitable.&#x0D; Conclusion and Recommendation: Community awareness regarding drug and substance among women and constant checkmating of the activities of street girls should be carried out.

ASSESSMENT OF FARMERS’ INFORMATION SOURCES UNDER SHELTERBELT PROJECTS IN THE FRONTLINE STATES OF NORTH WESTERN NIGERIA

The study analyzed farmers’ information sources under shelterbelt projects in the frontline States of North Western Nigeria. The respondents used were 450. Data was analyzed using descriptive statistics. Mean age of the respondents was 35 years; mean household size was 12 persons; mean farming experience was 12 years; and mean farm size was 6 hectares. About 67% of the respondents were married. Their sources of agricultural information of 29.1% was extension agents, radio accounted for 27.8%, family member (13.3%) and non-governmental organizations/community-based organizations (NGOs/CBOs) occupy 6.7%, print publications (4.4%) and internet (3.1%). Also, extension contact (30.0%), extension agents (25.5%) visited for two times, 18.9% were visited three (3) times, 11.1% were visited four (4) times annually, 7.8% were visited for five (5) times, and 6.7% were visited more than five (5) times annually. The results revealed that 61.1% listened to agricultural program through radio regularly. Majority (72.2%) watched/listened to agricultural program through television once in a week, 18.9% watched/listened twice a week and; 2.2% of the respondents were regular and 6.7% were irregular. Most (40.0%) preferred to radio program as their means of communication. Group meeting (extension agents/farmers) was only 5.1%, agriculture shows/field visit/tours occupy (11.1%) and extension agents interpersonal visit (8.9%), television (7.1%), newspaper and magazine (4.4%); and journals/conference proceedings and the internet services was 2.2%, respectively. The recommended conversion of the shelterbelts to recreational parks and tourism; reducing criminal activities like Indian hemp smoking, theft and kidnapping in the belt; and also, local communities should be allowed to participate in the management and sharing of revenue from the shelterbelts.

Study on the Characteristics of Cottonized Indian Industrial Hemp Fibers

ABSTRACT Hemp fiber is one of the natural bast fibers, mainly obtained from the hemp plant with the species of Cannabis sativa(L). In recent time, hemp fibers have received wide applicability in composite materials on account of their sustainability and low density compared with manmade fibers. Hemp fibers also have inherent mechanical, acoustic, thermal, and physical properties. Industrial hemp is a genetically modified form of C. sativa(L) plant containing low THC (Δ-9 Tetrahydrocannabinol) value of below 0.3%. In this study, an attempt has been made to cottonization of Indian industrial hemp fiber. The fibers were given an alkali treatment for the removal of non-cellulosic content. Three different levels of concentration, time and temperature were selected for the treatments on the Box-Behnken response surface design principle. The effect of alkali treatment on mechanical properties viz; length, strength, and weight loss and physical properties viz; diameter, surface were analyzed. This process has eliminated the Indian traditional tedious water retting and time-consuming process used on Hemp fibers. The resultant fiber obtained from the proposed process is textile grade quality and spinnable by the cotton dry spinning system. The effect of alkali treatment on the mechanical and physical properties was found significant.

Newly defined allergens in the WHO/IUIS Allergen Nomenclature Database during 01/2019-03/2021.

The WHO/IUIS Allergen Nomenclature Database (http://allergen.org) provides up‐to‐date expert‐reviewed data on newly discovered allergens and their unambiguous nomenclature to allergen researchers worldwide. This review discusses the 106 allergens that were accepted by the Allergen Nomenclature Sub‐Committee between 01/2019 and 03/2021. Information about protein family membership, patient cohorts, and assays used for allergen characterization is summarized. A first allergenic fungal triosephosphate isomerase, Asp t 36, was discovered in Aspergillus terreus. Plant allergens contained 1 contact, 38 respiratory, and 16 food allergens. Can s 4 from Indian hemp was identified as the first allergenic oxygen‐evolving enhancer protein 2 and Cic a 1 from chickpeas as the first allergenic group 4 late embryogenesis abundant protein. Among the animal allergens were 19 respiratory, 28 food, and 3 venom allergens. Important discoveries include Rap v 2, an allergenic paramyosin in molluscs, and Sal s 4 and Pan h 4, allergenic fish tropomyosins. Paramyosins and tropomyosins were previously known mainly as arthropod allergens. Collagens from barramundi, Lat c 6, and salmon, Sal s 6, were the first members from the collagen superfamily added to the database. In summary, the addition of 106 new allergens to the previously listed 930 allergens reflects the continuous linear growth of the allergen database. In addition, 17 newly described allergen sources were included.

The effect of different vegetable oils on energy content of table eggs yolk

Article Details: Received: 2020-08-31 | Accepted: 2020-11-16 | Available online: 2021-06-30 https://doi.org/10.15414/afz.2021.24.02.137-140 The aim of this study was to analyse the gross energy (GE) value of egg yolk from hens feed with different vegetable oils addition and based on gained results calculate the linear regression between GE and dry matter % (DM) in yolk. Total 66 hens Lohmann brown lite were divided in to 11 groups according to concentration and type of used vegetable oil (pumpkin oil 3%, flax seed oil 3%, indian hemp seed oil 2.5% and 5%, grape seed oil 2.5% and 5%, olive oil 2.5 and 5%, apricot seed oil 2.5% and 5%). Hens were in 40 th week of life and were housed per 6 in cage. From each group eight eggs were randomly selected. In total 88 egg yolks were separated and analysed for DM and GE content. The highest yolk GE was detected in group with 3% pumpkin oil addition (1639 kJ/100g) followed by groups with 2.5 and 5% indian hemp seed oil addition 1632 and 1632 kJ/100g, respectively. Difference between these three groups compared to yolk GE content (1584 kJ/100g) in control group was significant (P&lt;0.05). All other added oils increased the yolk GE (P&gt;0.05). GE concertation of yolk can be calculated as follows: GE = -17.34 + (32.73 * DM), R 2 = 0.819. Keywords: laying hens, egg yolk gross energy, regression References Antova, G. A. et al. (2019). Comparative analysis of nutrient content and energy of eggs from different chicken genotypes. Journal of the Science of Food and Agriculture, 99(13), 5890–5898. https://doi.org/10.1002/jsfa.9863 AOAC. (2000). Official methods of analysis AOAC. International 17th edition. Association of Analytical Communities, Gaithersburg. Benková, J. (2008). Artificial hatching of poultry is nothing simple. Slovenský chov, 13(6), 45-47. In Slovak. Gálik, B.et al. (2014). The effect of dietary Rhus coriaria L. on table eggs yolk nutrients composition. Acta fytotechnica et zootechnica, 17(3), 93–95. https://doi.org/10.15414/afz.2014.17.03.93-95 Herkeľ, R. et al. (2016). Fatty acid profile and nutritional composition of table eggs after supplementation by pumpkin and flaxseed oils. Acta Veterinaria Brno, 85(3), 277–283. https://doi.org/10.2754/avb201685030277 Hrnčár, C. &amp; Bujko, J. (2012). Effect of pre-storage incubation of hatching eggs on hatchability of poultry. Acta fytotechnica et zootechnica, 15(2), 34-37. http://www.acta.fapz.uniag.sk/journal/index.php/on_line/article/view/31 Javad, N. et al. (2011). Effects of amino acids and metabolizable energy on egg characteristics and broiler breeder performance. African Journal of Biotechnology, 10(49), 10066–10071. https://doi.org/10.5897/ajb11.1268 Noble, R. C. et al. (1996). Yolk lipids and their fatty acids in the wild and captive ostrich (Struthio camelus). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 113(4), 753–756. https://doi.org/10.1016/0305-0491(95)02097-7 Pearson, R. A. &amp; Herron, K. M. (1981). Effects of energy and protein allowances during lay on the reproductive performance of broiler breeder hens. British Poultry Science, 22(3), 227–239. https://doi.org/10.1080/00071688108447881 Pištěková, V. et al. (2011). The quality comparison of eggs laid by laying hens kept in battery cages and in a deep litter system. Czech Journal of Animal Science, 51(7), 318–325. https://doi.org/10.17221/3945-cjas Radu-Rusu, R. et al. (2014). Chemical features, cholesterol and energy content of table hen eggs from conventional and alternative farming systems. South African Journal of Animal Science, 44(1), 33. https://doi.org/10.4314/sajas.v44i1.5 Roe, M. et al. (2013). Nutrient analysis of eggs. Analytical Report (revised version). UK Government, Department of Health and Social Care. Retrieved December 10, 2020 from https://assets.publishing.service.gov.uk/government/uploads/system/ uploads/attachment_data/file/167973/Nutrient_analysis_of_ eggs_Analytical_Report.pdf Roztočilová, A. et al. (2018). Effect of purple wheat RU 687- 12 on performance parameters of laying hens at the end of the lay. In NutriNET 2018. Proceedings of reviewed scientific papers. Mendel University in Brno (pp. 92–97). Vojtaššáková, A. et al. (2000). Milk and eggs. Food nutrition tables. Výskumný ústav potravinársky, Bratislava. In Slovak. Van der Wagt, I. et al. (2020). A review on yolk sac utilization in poultry. Poultry Science, 99(4), 2162–2175. https://doi.org/10.1016/j.psj.2019.11.041 Zhang, Y. (2016). Studies on chicken hatchability and its relation with egg yolk metabolites. Dissertation thesis. GeorgAugust-University, Göttingen.

Students’ Perceptions on Drug Abuse and Academic Performance in Computer Science and Allied Subjects

Given the high rate of drug abuse in society, this study investigates how much of its knowledge is available to secondary school students. Also evaluated is the perceived effect of drug abuse on their academic achievement in subjects such as Computer science and allied subjects. A questionnaire was developed to obtain the respondents’ opinion with 304 responses analyzed for this study. This research reveals that the majority (97.4%) of students know about drug abuse and the harm it causes to health. The most abused drugs by the students surveyed are Alcohol (5.9%), Tobacco (3.3%), and Marijuana (Indian hemp) (2.3%). The hypotheses tested showed a statistically significant (p&lt;0.05) relationship between the type of school (public/private) a student attends and their tendency to abuse drugs, as well as how much knowledge is possessed by the students. We conclude that the academic performance of students can be negatively influenced by the abuse of drugs.