Independent Action Research Articles

Pot breaking in the wedding ritual of Don Cossacks: areal variations in the semantics and pragmatics of action

The breaking of dishes at weddings is known to all Slavs. It accompanies the most important stages of the ritual and symbolizes the change of the young couple’s social status. This action in the Don wedding tradition reveals a number of meanings, which are united by the symbolism of the ritual transition of the wedding participants, especially the bride, as well as the newlywed and their mothers. Often “pot beating” is part of the whole micro rites, including various manipulations with dishes and its fragments. Sweeping up the shards of broken crockery is the most stable element of the ritual, which can be combined with other components and performed as an independent action. In the memory of old residents of Don villages and farms for a long time there were actions (breaking a pot on the belly of the mothers-in-law, putting a chicken or a rooster into it, throwing crock-pots into the fire and jumping over it), behind which there are archaic folk beliefs related to the conclusion of marriage. However, the general orientation of breaking dishes in the Don tradition changes in the direction of increasing entertainment and competition.

Service-life multi-hazard risk assessment of structures: framework and application

Fragility and risk assessment of civil engineering structures against various design loads and their combinations need to be conducted to evaluate structural vulnerability. This is typically done at the design stage. However, during the service (design) life, it is important to investigate the change (increase) in the risk posed to the structure on account of multiple hazards. This article discusses a holistic novel framework that considers aging effects in addition to major or minor structural damage resulting from independent hazards. The framework is illustrated for independent action of earthquake and fire hazards on reinforced concrete (RC) buildings with varied occupancies while considering continuous structural deterioration on account of chloride- and carbonation-induced corrosion over a building’s service life. The change in the structure’s performance in the fire over its service life, in terms of incident risk and the resistance period, is evaluated for a combination of environmental effects and multi-hazard effects of earthquake and fire. The results from this study indicate that there is a significant increase in the risk of post-earthquake fire if continuous structural degradation due to environmental factors and minor damage due to earthquakes is not addressed.

Mechanistic understanding of the biochar-induced inhibition of growth and soil phosphorus solubilization by Bacillus megaterium

Due to the complex composition of biochar and the potential interactions between the components that jointly make up biochar, previous studies have always shown controversial results regarding impacts of biochar on soil phosphorus (P) solubilization. Using a nested experimental design, this study investigated the effects of different components of biochar, i.e. whole biochar (BC), dissolved biochar (DBC) and washed biochar (WBC) on the microbial-mediated P solubilizing process. The growth of Bacillus megaterium, acid/alkaline phosphatase activity, differential metabolites of bacteria and metabolic pathways were measured in treatments with biochar pyrolyzed at 200, 300, 400, 500 and 600 °C. Results showed that BC, DBC and WBC all negatively affected the growth and alkaline phosphatase activity of B. megaterium, and BC500/WBC500 and BC400/WBC400 had the greatest impacts. The negative effects of high-temperature biochar on cell proliferation were caused by persistent free radicals and included down-regulation of histidine metabolism by BC400 and interference in nucleotide metabolism by BC600. The impacts of low-temperature biochar on cell proliferation and alkaline phosphatase activity were highly related to the metal content. Using the independent action model, antagonistic effects were found between DBC and WBC on inhibiting B. megaterium growth, while these biochars had synergistic effects on inhibition of alkaline phosphatase activity. Overall, this study provides insights on how biochar adversely modulates microbial-mediated P solubilization.

Impact of emerging pollutants mixtures on marine and brackish phytoplankton: diatom Phaeodactylum tricornutum and cyanobacterium Microcystis aeruginosa

Pharmaceuticals and ionic liquids (ILs) are emerging as significant micropollutants with environmental presence and potential ecological impacts. The possible simultaneous occurrence of these two groups of pollutants in aquatic environments raises complex challenges due to their diverse chemical properties and potential for interactive effects. Given the documented widespread presence of pharmaceuticals and the emerging concerns about ILs, the study aims to evaluate the adverse effects of binary mixtures of imidazolium ionic liquid IM1-8C(CN)3 and two representatives of pharmaceuticals: antibiotic oxytetracycline (OXTC) and metabolite carbamazepine 10,11 epoxide (CBZ-E) on the brackish cyanobacterium Microcystis aeruginosa and the marine diatom Phaeodactylum tricornutum during chronic exposure experiments. A comprehensive approach was employed, incorporating various endpoints including oxidative stress, chlorophyll a fluorescence, detailed photoprotective and photosynthetic pigment profiles of target microorganisms to assess modes of action and identify the mixture effects of the selected substances.The observed alterations in pigment production affecting carotenoids synthesis in both selected species may be attributed to the differential impacts of these substances on the photosynthetic pathways and metabolic processes in the cyanobacterial and diatom cells. Changes in chlorophyll a fluorescence-specific parameters suggest impairment of the photosynthetic activity, particularly affecting the efficiency of photosystem II.The application of Concentration Addition (CA) and Independent Action (IA) mathematical models, complemented by the evaluation of Model Deviation Ratios (MDR), revealed predominantly antagonistic interactions within the studied mixtures. The findings of this study provide important insights into the effects of mixtures of organic micropollutants and their potential impact on environment including brackish and marine waters.

Empowering Communities to Act for a Change: A Review of the Community Empowerment Programs towards Sustainability and Resilience

At the global level, significant efforts have been made to address societal challenges and improve the lives of people and restore the planet’s ecosystems through sustainability and resilience programs. These programs, however, tend to be driven by governments, private sectors, and financial institutions, and therefore often lack a process of empowerment to ensure that the local communities can participate actively in co-designing and implementing these programs. More knowledge is needed on how to develop such programs and how the process of empowerment can be organized so that it supports in the long run sustainability transformation. Against this background, the paper explores the role of community empowerment programs as a critical tool for sustainability management strategies and practices. A semi-systematic review of 21 community empowerment programs for sustainability and resilience is conducted. The analysis reveals that the programs mostly aimed to address challenges such as the lack of education and capacity, limited access to basic services and resources, and poor governance and management. The programs initiators involve a diverse set of actors, especially through established partnerships and networks. Most of the programs address the specific needs of vulnerable or marginalized groups or communities. The structure of the programs typically follows a phased methodological approach, beginning with awareness-raising and problem identification, followed by capacity building that allows for making decisions collaboratively and for co-creating innovative solutions based on local knowledge and values. Also, monitoring and evaluation of transformative impact are mentioned as important structural elements. Specifically, the analysis highlights four main focus areas of empowerment: (1) capacity building, (2) self-reliance, control, ownership, responsibility, and independence, (3) participation, engagement, and collective action, and (4) integration of local knowledge and values. However, there is no one-size-fits-all approach to such programs. Instead, successful empowerment programs towards sustainability depend on a deep understanding of local contexts and the ability to tailor strategies to meet specific community needs. The review also identified knowledge gaps that require further investigation to enhance the effectiveness of empowerment programs for both people and nature.

Independent effects of testosterone, estradiol, and sex chromosomes on gene expression in immune cells of trans- and cisgender individuals.

The origins of sex differences in human disease are elusive, in part because of difficulties in separating the effects of sex hormones and sex chromosomes. To separate these variables, we examined gene expression in four groups of trans- or cisgender individuals: XX individuals treated with exogenous testosterone (n=21), XY treated with exogenous estradiol (n=13), untreated XX (n=20), and untreated XY (n=15). We performed single-cell RNA-sequencing of 358,426 peripheral blood mononuclear cells. Across the autosomes, 8 genes responded with a significant change in expression to testosterone, 34 to estradiol, and 32 to sex chromosome complement with no overlap between the groups. No sex-chromosomal genes responded significantly to testosterone or estradiol, but X-linked genes responded to sex chromosome complement in a remarkably stable manner across cell types. Through leveraging a four-state study design, we successfully separated the independent actions of testosterone, estradiol, and sex chromosome complement on genome-wide gene expression in humans.

Fluorescent nanoplastics increase the toxic effects of Graphene oxide nanoparticles in freshwater algae Scenedesmus obliquus

The increased usage of Graphene oxide (GO) in various industrial applications led to their entry into freshwater systems. Other secondary contaminants like nanoplastics (NPs) often co-exist with GO in the environment. This study examines the possible role of fluorescent nanoplastics (FNPs) in modifying the toxic effects of GO on freshwater algae Scenedesmus obliquus. Selected concentrations of GO (0.1, 1, and 10 mg L−1) were combined with a fixed concentration of FNPs (1 mg L−1) to perform combinational toxicity tests on algae. FNPs significantly enhanced the toxic effects of GO in the mixtures in comparison with the pristine GO. In addition to the cytotoxic effects, oxidative stress parameters like total ROS generation and malondialdehyde (MDA) production also increased in the case of the combined pollutants. The antioxidant enzymatic activities like catalase (CAT) and superoxide dismutase (SOD) in the cells were also assessed. Algal exposure to the pristine pollutants and their mixture led to a notable decrease in photosynthetic activities in the cells, with the mixed pollutants aggravating the loss of activity. The interactive toxic effects of the contaminants when present in mixtures were evaluated using Abbotts' Independent action modelling. Furthermore, optical microscopic images revealed the morphological changes in the algal cells after exposure to the contaminants both in the pristine and combined forms.

New binary mixtures of fungicides against Macrophomina phaseolina: Machine learning-driven QSAR, read-across prediction, and molecular dynamics simulation

Quantitative Structure-Activity Relationship (QSAR) analysis greatly enhances the development and research of pesticides. This study employed Multiple Linear Regression (MLR), machine learning (ML), and read-across (RA) approaches to investigate the combined effects of binary mixtures of fungicides on Macrophomina phaseolina. Using the Fixed Ratio Ray Design (FRRD) method, 75 binary mixtures of six frequently used fungicides were generated, with many exhibiting additive interactions as indicated by the Concentration Addition (CA) and Independent Action (IA) models. The QSAR analysis revealed that Support Vector Regression (SVR) and Gaussian Process Regression (GPR) models were the most effective, outperforming the Least Squares Kernel (LSK), MLR, and RA methods. SVR achieved an outstanding R2 of 0.95 and Q2LMO of 0.81, whereas GPR demonstrated values of 0.93 and 0.81 for the same metrics. Internal and external validation confirmed the reliability and generalizability of these models, suggesting they could be applied to a wider array of data. Moreover, Molecular Dynamics (MD) simulations showed that the effects of the fungicides are linked to physiological mechanisms rather than intermolecular interactions within their formulations. This study establishes a robust framework for creating potent fungicide combinations that improve disease management efficacy while promoting environmental sustainability and reducing the chemical load to mitigate negative impacts.

Development of the Volcanic Disaster Risk Reduction Education Program Using the ICT Tool “YOU@RISK Volcanic Disaster Edition” —Practical Verification at a Junior High School in the Mt. Nasu Area—

This study developed a volcanic disaster risk reduction education program that aims to help junior high school students visually understand the risks of volcanic eruptions using map information and acquire independent assessments and actions to protect their own lives from eruption disasters. First, this program analyzed the present state, problems, and educational needs of volcanic disaster risk reduction education in Japan, and thereafter designed the learning content to fuse information and communication technology (ICT) education based on the Global and Innovation Gateway for All (GIGA) School Initiative promoted by Japan with disaster risk reduction education from a geographical viewpoint. We adopted the ADDIE model from instructional design theory to develop the program. An important feature of this program is the realization of geography learning using the “YOU@RISK Volcanic Disaster Edition,” a web-based GIS application developed by the National Research Institute for Earth Science and Disaster Resilience (NIED), as an ICT tool. Learners use YOU@RISK on their tablets and perform operations such as visualizing the risk range in the event of a volcanic eruption and searching for appropriate evacuation sites and routes, while acquiring the knowledge and skills necessary for geographical literacy and emergency response actions. The program is able to realize interactive learning, which is a step ahead of education that relies on conventional paper-based hazard maps. Among active volcanoes nationwide, Mt. Nasu was selected as target volcano for this study. In addition, a junior high school located in the area surrounding Mt. Nasu, Nasu Junior High School in Nasu Town, Tochigi Prefecture, was selected as the target school. The program was implemented by teachers using tablets provided for each student at the school. We evaluated the effectiveness of the program by measuring and analyzing the effects of learning through the practice. Consequently, the effectiveness of the volcanic disaster risk reduction education program using ICT was confirmed.

Проявление агентности студенчества в проектном обучении: кейс федерального университета

According to experts, there will be a growing demand for hybrid and multidisciplinary specialists with advanced cognitive skills, emotional intelligence, and technological and social competence by 2030. These professionals will be able to make independent and innovative decisions in a rapidly changing labor market. The system of higher professional education faces the challenge of addressing the quantitative and qualitative shortage of young professionals. In this context, the topic of modernizing personnel training includes the introduction and active promotion of project-based learning. Project-based training should promote the agency of students as young professionals by transferring them within the framework of training from passive behavior to active independent actions, effective interaction with the professional community, disclosure of leadership potential, development of initiative and readiness to design new interdisciplinary solutions. The manifestations of student agency in project-based learning is an urgent issue on the research agenda and management practice of universities. The aim of the study was to investigate the key features of UrFU students' agency, as manifested in their project-based learning experiences. The article presents the findings from a survey conducted among students (N=1,070) using a quota sampling method in 2024 (including sex, training level and major subject). The survey error does not exceed 3 percent. According to the survey results, the relevance of key features of agency and project-based learning was established. It was empirically proved that project-based learning encourages independent, proactive behavior, promotes the development of leadership qualities, and enhances students' innovative potential.

Decoding the Role of Nuclear Sirtuins in Parkinson's Pathogenesis.

Parkinson's disease (PD) is the second most prevailing degenerative disease that deals with dopaminergic neuronal loss and deficiency of dopamine in SNpc and striatum. Manifestations primarily include motor symptoms like tremor, rigidity, and akinesia/dyskinesia along with some nonmotor symptoms like GI and olfactory dysfunction. α-Synuclein pathogenesis is the major cause behind progression of PD; however there are many underlying molecular mechanisms behind the pathophysiology of PD. Sirtuins are small molecular deacetylases that have an imperative role in pathology of such neurodegenerative disorders like PD. Sirtuins are majorly classified according to their location; nuclear (SIRT1,7,6), mitochondrial sirtuins (SIRT3-5), and cytosolic (SIRT2). These actively take part in pathological development and possess independent actions. In this review, the role of nuclear sirtuins is individualistically explored for better understanding of PD pathology and development of advanced therapeutics targeting sirtuins.

Non-conventional endpoints show higher sulfoxaflor toxicity to Chironomus riparius than conventional endpoints in a multistress environment

Evidence grows that standard toxicity testing might underestimate the environmental risk of neurotoxic insecticides. Behavioural endpoints such as locomotion and mobility have been suggested as sensitive and ecologically relevant additions to the standard tested endpoints. Possible interactive effects of chemicals and additional stressors are typically overlooked in standardised testing. Therefore, we aimed to investigate how concurrent exposure to environmental stressors (increased temperature and predation cues) and a nicotinic acetylcholine receptor (nAChR)-modulating insecticide (‘sulfoxaflor’) impact Chironomus riparius across a range of conventional and non-conventional endpoints. We used a multifactorial experimental design encompassing three stressors, sulfoxaflor (2.0–110 µg/L), predation risk (presence/absence of predatory cues), and elevated temperature (20 °C and 23 °C), yielding a total of 24 distinct treatment conditions. Additional stressors did not change the sensitivity of C. riparius to sulfoxaflor. To assess potential additive effects, we applied an Independent Action (IA) model to predict the impact on eight endpoints, including conventional endpoints (growth, survival, total emergence, and emergence time) and less conventional endpoints (the size of the adults, swimming abilities and exploration behaviour). For the conventional endpoints, observed effects were either lower than expected or well-predicted by the IA model. In contrast, we found greater than predicted effects of predation cues and temperature in combination with sulfoxaflor on adult size, larval exploration, and swimming behaviour. However, in contrast to the non-conventional endpoints, no conventional endpoints detected interactive effects of the neurotoxic insecticide and the environmental stressors. Acknowledging these interactions, increasing ecological context of ecotoxicological test systems may, therefore, advance environmental risk analysis and interpretation as the safe environmental concentrations of neurotoxic insecticides depend on the context of both the test organism and its environment.

Threat in the context of identifying the victim

It is indicated that an important feature of the injured person, which allows to distinguish him from other persons, is related to the infliction of damage. The etymological and semantic analysis of the term «victim» shows that damage is an integral feature of a victim. The article examines the possibility and expediency of recognizing a person as a victim in the criminal law field in the event of a threat to cause harm to him. Provisions of the current Criminal Code of Ukraine, the project of the new Criminal Code of Ukraine, national judicial practice are taken into account. It is argued that in context of the injured person, term damage is understood in two meanings: 1) narrowly (includes only real damage); 2) in a broad sense (includes both damage and the danger/threat of causing it, as well as the threat). At the same time, attributing the threat as an independent action to a type of harm, as suggested by authors of the draft Criminal Code of Ukraine, seems debatable, because in such a case the term «threat» will denote both an action and a consequence. It is also worth paying attention to whether there is a need to use both the term «threat» and the term «danger» in the draft Criminal Code of Ukraine. It has been established that the term «threat» in the context of the definition of the victim means both actual moral or other types of harm to him, as well as the threat/danger of his task. It has been proven that victim in a certain composition of a criminal offense can be characterized not only by harm in its narrow sense, but also by threat or danger of its occurrence, that is, harm in the broad sense, as well as the threat of harm. The threat in the context of the criminal-legal understanding of victim is important, first of all, if there is no implementation. In case of the implementation of a threat, which is provided as an independent crime and is a stage of a single criminal activity, united by a single intention and aimed at causing damage to the same object, qualification under the article providing for responsibility for the threat is not required, and therefore and there is no need to establish the victim of the threat. If a threat is a way of committing an act, then the victim is determined in the context of act in general, taking into account the threat as a way of committing it.

Ecotoxicological study of seven pharmaceutically active compounds: Mixture effects and environmental risk assessment

Pharmaceutically active compounds (PhACs) have been detected in several aquatic compartments, which has been of environmental concern since PhACs can cause adverse effects on the aquatic ecosystem at low concentrations. Despite the variety of PhACs detected in surface water, ecotoxicological studies are non-existent for many of them, mainly regarding their mixture. In addition, water bodies can continuously receive the discharge of raw or treated wastewater with micropollutants. Thus, PhACs are subject to mixture and interactions, potentiating or reducing their toxicity. Therefore, the present study evaluated the toxicity on Aliivibrio fischeri of seven PhACs, which still needs to be explored in the literature. The effects were evaluated for the PhACs individually and for their binary and tertiary mixture. Also, the experimental effects were compared with the concentration addition (CA) and independent action (IA) models. Finally, an environmental risk assessment was carried out. Fenofibrate (FEN), loratadine (LOR), and ketoprofen (KET) were the most toxic, with EC50 of 0.32 mg L-1, 6.15 mg L-1 and 36.8 mg L-1, respectively. Synergistic effects were observed for FEN + LOR, KET + LOR, and KET + FEN + LOR, showing that the CA and IA may underestimate the toxicity. Environmental risks for KET concerning algae, and LOR e 17α-ethynylestradiol (EE2) for crustaceans and fish were high for several locations. Besides, high removals by wastewater treatment technologies are required to achieve the concentrations necessary for reducing KET and LOR risk quotients. Thus, this study contributed to a better understanding of the toxic interactions and environmental risks of PhACs.

Metal Mixture Effects of Ni, Cu, and Zn in a Multispecies, Two-Trophic-Level Algal-Daphnid Microcosm Can Be Predicted From Single-Trophic-Level Effects: The Role of Indirect Toxicity.

Effect assessments of metals are mostly based on single-metal, single-species tests, thereby ignoring metal-mixture effects and indirect effects through species interactions. We tested the combined effects of metal and species interactions in two-trophic algal-daphnid microcosms. Metal-mixture effects on daphnid communities may propagate from effects on the generally more sensitive algal communities. Four different algal communities (three species each), with and without addition of the same daphnid community (three species) were exposed to single metals and one metal mixture (17:17:51 µg/L Ni:Cu:Zn). Daphnid densities were negatively affected by metals in the two-trophic test, the magnitude of which depended on the algal community composition. Algal densities were overall positively affected by the metals in the two-trophic test but negatively in the single-trophic test, illustrating an indirect positive effect in the two-trophic system due to a reduced grazing pressure. Metal effects on daphnid communities in the two-trophic test (day 21) were correlated with metal effects on the single-trophic-level algal communities during exponential growth (R2 = 0.55, p = 0.0011). This finding suggests that metal effects propagate across trophic levels due to a reduced food quantity. However, the indirect positive effects on algal densities, resulting in abundant food quantity, suggests that metal effects can also propagate to daphnids due to a reduced food quality (not measured directly). Metal-mixture interactions on daphnid densities varied during exposure, but were additive or antagonistic relative to independent action when final daphnid densities were considered (day 56). This suggests stronger indirect effects of the mixture compared with the single metals. Overall, our study highlights the dynamic aspect of community-level effects, which empirical reference models such as independent action or concentration addition cannot predict. Environ Toxicol Chem 2024;43:2350-2364. © 2024 SETAC.

Effects of Non-steroidal Anti-inflammatory Drugs (Ibuprofen and Diclofenac) and Their Mixtures on the Growth of Three Green Algae

Ibuprofen (IBU) and diclofenac (DFN) are the most widely consumed non-steroidal anti-inflammatory (NSAIDs) worldwide. Since they are not completely metabolized in the human body, the unaltered active ingredients can reach aquatic ecosystems through domestic effluents. The aim of this study was to analyse the toxic effects of IBU and DFN, individually and in mixtures, on three freshwater green algae strains, the international standard strain Raphidocelis subcapitata and two native strains, Ankistrodesmus fusiformis and Tetradesmus obliquus. Bioassays were carried out according to the 72-h algal growth inhibition test. The concentration-response curve of the mixture was compared to predicted effects based on both the concentration addition (CA) and the independent action (IA) models. The two drugs tested individually were toxic to all three algal strains, with EC50 values <100 mg L-1. According to these values, the most sensitive strain was R. subcapitata (IBU = 20 mg L-1 and DFN = 8 mg L-1). The most resistant strain was T. obliquus (IBU = 74.1 mg L-1 and DFN = 92.7 mg L-1) and the A. fusiformis strain showed intermediate sensitivity (IBU = 26.7 mg L-1 and DFN = 14.6 mg L-1). The mixtures showed a synergistic effect on R. subcapitata, an additive effect on A. fusiformis, and an antagonistic effect on T. obliquus. Neither the CA nor the IA model was appropriate for predicting the toxicity of the mixtures. In conclusion, IBU and DFN showed toxic effects on the algae growth at the concentrations tested. Individual and mixed toxicity was different according to the species. These differences could be species-specific, showing the importance of including native strains in ecotoxicological studies to evaluate the potential effects of pollutants in regional aquatic environments.

A pan-cancer screen identifies drug combination benefit in cancer cell lines at the individual and population level

Combining drugs can enhance their clinical efficacy, but the number of possible combinations and inter-tumor heterogeneity make identifying effective combinations challenging, while existing approaches often overlook clinically relevant activity. We screen one of the largest cell line panels (N= 757) with 51 clinically relevant combinations and identify responses at the level of individual cell lines and tissue populations. We establish three response classes to model cellular effects beyond monotherapy: synergy, Bliss additivity, and independent drug action (IDA). Synergy is rare (11% of responses) and frequently efficacious (>50% viability reduction), whereas Bliss and IDA are more frequent but less frequently efficacious. We introduce "efficacious combination benefit" (ECB) to describe high-efficacy responses classified as either synergy, Bliss, or IDA. We identify ECB biomarkers invitro and show that ECB predicts response in patient-derived xenografts better than synergy alone. Our work here provides a valuable resource and framework for preclinical evaluation and the development of combination treatments.

Equations for estimating binary mixture toxicity: 3-methyl-2-butanone with a series of electrophiles.

Mixture toxicity was determined for 32 binary combinations. One chemical was the non-reactive, non-polar narcotic 3-methyl-2-butanone (always chemical A) and the other was a potentially reactive electrophile (chemical B). Bioluminescence inhibition in Allovibrio fischeri was measured at 15-, 30-, and 45-minutes of exposure for A, B, and the mixture (MX). Concentration-response curves (CRCs) were developed for each chemical and used to develop predicted CRCs for the concentration addition (CA) and independent action (IA) mixture toxicity models. Also, MX CRCs were generated and compared with model predictions using the 45-minute data. Classification of observed mixture toxicity used three specific criteria: 1) predicted IA EC50 vs. CA EC50 values at 45-minutes, 2) consistency of 45-minute MX CRC fit to IA, CA, or otherwise at three effect levels (EC25, EC50 and EC75), and 3) the known/suspected mechanism of toxicity for chemical B. Mixture toxicity was then classified into one of seven groupings. As a result of the predicted IA EC50 being more toxic than the predicted CA EC50, IA represented the greater toxic hazard. For this reason, non-sham MXs having toxicity consistent with CA were classified as being "coincident" with CA rather than mechanistically-consistent with CA. Multiple linear regression analyses were performed to develop equations that can be used to estimate the toxicity of other 3M2B-containing binary mixtures. These equations were developed from the data for both IA and CA, at each exposure duration and effect level. Each equation had a coefficient of determination (r2) above 0.950 and a variance inflation factor <1.2. This approach can potentially reduce the need for mixture testing and is amenable to other model systems and to assays that evaluate toxicity at low effect levels.

THE PROBLEM OF DEPENDENCE ON SOCIAL WELFARE BENEFITS IN THE EXPERIENCE OF SOCIAL WORKERS

ABSTRACT: Aim: To learn and understand the problem of dependence on social assistance of clients of this institution on the basis of professional experience of social workers. Methods: The research used a qualitative research method - a collective case study. A total of 15 problem-focused and partially guided individual interviews were conducted with social workers, in three selected provinces in Poland. Results: The research shows that the phenomenon of clients' dependence on social welfare benefits is an important professional challenge experienced by social workers. According to the respondents, the social welfare system in Poland only partially activates the wards to take independent action to overcome difficult life situations, which further contributes to the creation and perpetuation of claimant and passive attitudes towards social welfare institutions. Coclusion: The presented results of the research prompt reflection on the role and function of social welfare benefits granted. The activities of social welfare institutions should aim to maximize the independence of their clients through social and professional activation, mobilization of human forces inherent in the environment, and revealing, stimulating and consolidating these forces. Oryginality: The article represents an area of analysis that is not often discussed in the scientific space. There is a lack of studies or research reports on this topic. The text presented here is one of the first to address the issue of research in the field of social welfare addiction of its clients from the perspective of professional social services. Keywords: social assistance, social worker, addiction, helplessness, exclusion, marginalization, passivity, entitlement.

Bacterial toxicity of Acetaminophen and Edaravone, and their binary mixtures: experimental and predicted values using traditional and novel Van Laar-based models

In recent years, the presence of Pharmaceutical Active Compounds (PhACs) in ecosystems has become a serious environmental problem due to their capacity to induce harmful effects at extremely low concentrations in both humans and wildlife. Water treatment plants have not been designed to remove these types of compounds efficiently. Thus, the detection of these pollutants is essential to evaluate their negative impacts and is one of the emerging issues in environmental chemistry. The main objective of this study is to determine the bacterial toxicity of two PhACs (both individually and as a mixture) through the quantification of bioluminescence inhibition in the marine bacteria Aliivibrio fischeri, a commonly used method in short-term toxicity tests. In this work, Acetaminophen and Edaravone, two drugs approved by the Food and Drug Administration, have been studied. The acute toxicity of these PhACs has been tested at two exposure times (5 and 15 min) and different concentrations, by estimation of the median effective concentration (EC50) for each individual compound or in combination at different concentrations. Moreover, the EC50 of the binary mixtures Acetaminophen/Edaravone have been forecast using two traditional predictive models, Concentration Addition and Independent Action. The results show that toxicity decreases with exposure time and depends on the concentration tested. Furthermore, a novel semi-empirical Van Laar-based model has been proposed and validated with the experimental data from this study and literature data, obtaining satisfactory estimations of the EC50 for binary mixtures.