Incremental Ratio Research Articles

Cost-effectiveness of C-reactive protein point of care testing for safely reducing antibiotic consumption for acute exacerbations of chronic obstructive pulmonary disease as part of the multicentre, parallel-arm, open, individually randomised, controlled PACE trial

ObjectivesMany patients presenting with acute exacerbations of chronic obstructive pulmonary disease (AECOPD) in primary care do not benefit from antibiotics. Excessive use wastes resources, promotes antimicrobial resistance and can harm...

The role of incremental stress ratio in mechanical behavior and particle breakage of calcareous sand

The role of incremental stress ratio in mechanical behavior and particle breakage of calcareous sand

Comparison of the Dynamic Characteristics of Tuned Liquid Column Dampers with Different Elbow Forms

This study aims to examine experimentally the damping performance of Tuned Liquid Column Dampers (TLCD) by considering different elbow forms. Experiments carried out within the scope of the study point out the dynamic characteristics of TLCD, which has 45 (open) and 90 (closed) degree-elbow forms with theoretical angular frequencies that are the same. Experiments consist of ±5 and ±10 mm harmonic excitation amplitude to observe the relationship between the damping ratio and head loss coefficient with the mass ratio of the TLCD. These experiments with an incremental mass ratio of 0.05% from 0.80% to 1.00% and with an incremental mass ratio of 0.10% from 1.00% to 1.20% had been carried out in detail. Mass and stiffness Modification Factors (MF) are suggested to minimize the difference between numerical and experimental results. Determined MFs are used to examine the earthquake performance of TLCD on a Single-Degree-of-Freedom (SDOF) system. It is shown that the open elbow has an advantage of approximately 25% over the close elbow under earthquake performance on the SDOF system.

Homogeneous Mo1−xRexS2 monolayer and lateral 1T’@2H heterostructures for significantly enhanced and highly reproducible SERS responses and mechanistic insights

As a promising surface-enhanced Raman scattering (SERS) substrate, two-dimensional (2D) nanomaterials ensure the uniformity and reproducibility of the SERS response. However, their extremely flat surfaces are not beneficial for high-capacity binding of analytes and significant enhancement of Raman signals. In this contribution, monolayer Mo0.27Re0.73S2 alloys with distorted lattice and modulated optoelectronic structure are facilely fabricated by space-confined chemical vapor deposition (CVD), exhibiting a significantly enhanced SERS performance with an enhancement factor of 2.0 × 1011. As demonstrated, the continuous increment of Re ratio results in a phase transition from 2H to 1T’ for homogeneous Mo1−xRexS2 monolayers. As a result, the 1T’-phase Mo0.27Re0.73S2 alloys achieve a sensitive detection of down to 10−15 M for Rhodamine 6G while the value on MoS2 or ReS2 is only 10−9 M. Additionally, the alloy-based SERS substrate demonstrates excellent SERS detection reproducibility, long-term stability, and universality. Referring to the space-confined CVD, a dynamics-controlled process is further employed for the in-situ fabrication of 1T’@2H Mo1−xRexS2 heterostructures, providing a new approach and insight for facilely building varied phases-based lateral heterostructures by the control of composition ratio. Overall, this work demonstrates the enormous potential of alloy engineering in improving the SERS performance of TMDs and the convenient fabrication of out-of-phase TMD alloys.

Enhancement of PV and concentered PV panels using evaporative cooling during summer and winter seasons: A case study in Egypt

Numerous efforts are made to comprehend the performance evolution of photovoltaic (PV) panels. The elevated surface temperature causes a deterioration in PV panels' performance, hindering the expected increase in harvested energy associated with integrating solar reflectors. In this context, the year-round efficacy of cooling PV panels supported by reflective mirrors is investigated to assess the effectiveness of solar reflector implementation. A conventional PV panel, a CPV (cooled PV panel), and a CCPV (concentrated cooled PV panel) were compared side-by-side to determine their relative performance and energy productivity. Evaporative cooling pads are utilized to chill the two modified PV systems by varying the coolant flow rate during the summer and winter seasons of 2022 and 2023, respectively, in the environmental conditions of the Tenth of Ramadan City, Egypt. The findings indicate that the average daily power increment ratio (PIR) for CPV ranges from 4.7 % up to 7.4 %, while CCPV ranges from 6.7 % to 12 % for different climate conditions year-round. Finally, changing the setup configuration from CPV to CCPV increased the (PIR) 1.5 times during the summer tests and 1.7 times during the winter tests.

High-risk human papillomavirus testing for cervical cancer screening in Uganda: Considering potential harms and benefits in a low-resource setting.

The World Health Organization supports both the screen-and-treat (ST) approach and the screen, triage and treat (STT) approach to cervical cancer screening using high-risk human papillomavirus (hrHPV) testing. For Uganda, the sequence of hrHPV-ST and hrHPV-STT could be similar, with visual inspection with acetic acid (VIA) after positive hrHPV tests in both. To consider potential tradeoffs (overtreatment in ST versus missed cancer cases in STT), we compared hrHPV-STT with VIA triage (STT-VIA), and STT with HPV 16/18 genotyping risk stratification, to hrHPV-ST for Uganda, in terms of overtreatment, cervical cancer incidence, and life years, for the general female population of Uganda. A microsimulation model of cervical cancer was adapted. Incremental benefit-harm ratios of STT were calculated as ratios of prevented overtreatment to reduced life years, and to increased cancer cases. Additional scenarios with 20% difference in intra- and inter-screening follow-up between ST and STT were modeled. Both STT strategies resulted in life year losses on average compared to ST. STT-VIA prevented more overtreatment but led to increased cervical cancer incidence and life year losses. STT-G-VIA resulted in better harm-benefit ratios and additional costs. With better follow-up, STT prevented overtreatment and improved outcomes. For Uganda, the STT approach appears preferrable, if the screening sequences of hrHPV-based ST and STT are similar in practice. While VIA triage alone would reduce overtreatment the most, it could also result in more cancer cases. Risk stratification via genotyping could improve STT. Potential follow-up differences and resource availability should be considered by decision-makers when planning Uganda's hrHPV-based screening strategy.

Evaluation framework for bitumen-aggregate interfacial adhesion incorporating pull-off test and fluorescence tracing method

The interfacial adhesion between bitumen and aggregate is crucial to maintain the mechanical performance of asphalt pavement. Numerous indicators characterize the bonding ability of bitumen to aggregate; however, indicators based on limited conditions are used infrequently to effectively evaluate the interfacial adhesion between bitumen and aggregate. This study introduces a method to assess adhesion and moisture damage resistance at the bitumen-aggregate interface by combining the pull-off test with the fluorescence tracing method. The improved pull-off test considers different bitumen film thicknesses, ambient temperatures, material combinations, and moisture damage conditions, and the fluorescence tracing method and surface energy parameters are utilized to precisely detect the extent of bitumen adherence to the aggregate. The findings of this study are summarized as follows. (1) The bitumen stripping ratio characterizes the influence of ambient temperature and bitumen film thickness, and the pull-off strength/work quantifies the influence of ambient temperature and material combination. (2) The indicators derived from surface free energy theory exhibit lower sensitivity to material combinations compared with the pull-off performance indicators, with a coefficient of variation for both adhesion and stripping work of approximately 6 % compared to 14.29 %–70.13 % for the pull-off performance indicators. (3) The pull-off strength indicator dominates the pull-off performance evaluation, with the bitumen stripping ratio necessary at 0°C and pull-off work significant at 40°C to evaluate adhesive properties. In addition, the bitumen stripping ratio increment should be considered alongside pull-off strength loss ratio when estimating anti-moisture damage performance. (4) The fluorescence tracing method shows great potential in terms of enhancing the accuracy of bitumen stripping ratio detection in the pull-off test, with bitumen stripping ratio discrepancies at 0°C–40°C ranging from 0.98 % to 7.16 % for 70#-Limestone and 2.10–9.21 % for 70#-Granite. The methodology presented in this study represents a promising framework to determine the interfacial adhesive properties of asphalt mixtures with high accuracy.

The economic impact of dry port investment in Indonesia: A case study of Bangil, Pasuruan District, East Java Province

Acknowledging the pivotal role of ports and dry ports in shaping a nation’s economic landscape, this study explores the economic impact of port development in East Java, specifically Bangil Dry Port in Pasuruan over at least 30 years of business operational process. This study pioneers the quantification of gross value added, investment multiplier effect, tax contribution, and Creating Shared Value (CSV) resulting from dry port investment. The method used for calculating multiplier investment is Keynesian multiplier approach and for labor absorption is Incremental Labor Output Ratio (ILOR). Field surveys were conducted to determine the condition of Bangil, and secondary data on the growth of container flows at Port of Tanjung Perak was used as a reference for growth in Bangil. Overall, these results show that Bangil Dry Port investment will generates positive impact both for government and society in general, but also the economy of East Java and Pasuruan District in particular. These results offer practical guidance for decision-makers in optimizing public funds allocation, while also laying the groundwork for future scholars to conduct comprehensive societal-scale cost-benefit analyses of strategic port investments, contributing to a deeper understanding of the economic implications.

Incremental Cost Benefit Ratio (ICBR) of Poly House and Open Field Conditions in Cabbage (Brassica oleracea var. capitata) after Chemical Intervention

This study evaluates the Incremental Cost Benefit Ratio (ICBR) between Poly house and Open field conditions in cabbage cultivation chemical intervention. The research aimed to compare the economic feasibility of adopting poly house technology over traditional open field methods, considering costs and benefits associated with chemical inputs. A comparative cost analysis was conducted to assess production expenses like costs for insecticidal treatments and labor charges. Yield data from both environments were collected to determine output differences and subsequent economic returns. Results indicate that Incremental cost benefit ratio of cabbage heads yields to the cost of treatments in poly house and open field presented as there was a significant superior difference in ICBR ratio of poly house with 1: 0.946 as compared to open field with 1: 0.639 of cabbage heads. The economic analysis revealed that the higher yields and quality achieved in poly house conditions due to less dissipation and undisturbed environmental factors on insecticide applied. Resulting in a favorable incremental Cost benefit ratio compared to open field cultivation.

Innovative Strategies for Controlling Sorghum Shoot Fly: Seed Treatments and Botanical Insecticides

Aim: To assess the efficacy of various treatments for managing sorghum shoot fly, Atherigona soccata Rondani, and their effects on plant vigour, growth, yield, and Incremental Cost-Benefit Ratio (ICBR). Study Design: A field experiment with a Randomized Block Design, involving three replications and nine treatments. Place and Duration of Study: Chaudhary Charan Singh Haryana Agricultural University, Hisar (29.1492° N, 75.7217° E), during the Kharif season of 2020. Methodology: Sorghum variety Swarna was sown in August 2020. Treatments included Imidacloprid 70WS, Thiamethoxam 30FS, Neem oil, Karanj oil, and their combinations. Treatments were applied at 7 and 17 days after emergence. Data on plant stand, egg counts, dead hearts (%), plant height, green fodder yield, and ICBR were analyzed. Results: Thiamethoxam 30FS @ 10ml/kg + neem oil @ 2% showed the best performance, significantly reducing shoot fly infestation (19.98%) and enhancing plant vigour (1.00), height (86.93 cm), and yield (309.60 q/ha). Neem oil @ 2% was more effective than Karanj oil @ 2%. Imidacloprid 70WS had the highest Incremental Cost Benefit Ratio (ICBR) (1:21), while Karanj oil @ 2% had the lowest (1:0.78). Conclusion: Thiamethoxam 30FS @ 10ml/kg + neem oil @ 2% was the most effective for managing shoot fly and improving crop performance. Imidacloprid 70WS offered the highest economic benefit, indicating its cost-effectiveness in pest management.

Evaluation of Physical and Sensory Properties of Biscuits Made from Blended Wheat Flour and Pumpkin Powder

Biscuit is a type of confectionery popular among all age groups and able to change to become a complete food with necessary nutrients. Pumpkin is a nutritious vegetable that has high post-harvest losses in Sri Lanka. The aim of this research was to analyze the composition of pumpkin powder and evaluate the physical and sensory properties of biscuits made from blended wheat flour and pumpkin powder. Analyses revealed that moisture, protein, fat, ash, crude fiber, and carbohydrate content of pumpkin powder were 8.5%, 5.2%, 1.2%, 3.4%, 4% and 77.7% respectively. Biscuits were made by replacing wheat flour with pumpkin powder at different levels as 0, 5, 10, 20, 30, and 40% (w/w) in the standard formulation. When the percentage of pumpkin powder increased from 10 to 40%, the thickness of biscuits decreased significantly compared to control (0%) There was a significant increment in the spread ratio of biscuits between 10 and 40% levels of pumpkin powder when compared with the control. While 10 to 40% replacement levels of pumpkin powder resulted in significant increase in biscuit hardness, it decreased moisture content significantly compared to control. The color of the biscuits was assessed in terms of lightness, redness, and yellowness. When increasing the pumpkin powder percentage in biscuit, lightness reduced, while redness and yellowness increased gradually. Consumer acceptability test on sensory attributes indicated 5% (w/w) level of pumpkin powder was the most preferred replacement level. The study concludes that pumpkin powder has the potential to be used in biscuit applications.

Impact of biopolymer-based Trichoderma harzianum seed coating on disease incidence and yield in oilseed crops

The use of microbe-based biological control for crop pests is recognized as an environmentally safe substitute for conventional chemical pesticides. However, the practical application of microbial inoculants in large-scale agriculture is underexplored, impeding their widespread commercial adoption. This study addresses the scarcity of research on effective delivery methods for microbial inoculants, particularly through seed coating, which has the potential to be a cost- and time-efficient strategy in crop management. In this research, the Trichoderma harzianum strain Th4d, a biological control agent (BCA), was incorporated into specially formulated biopolymeric compositions based on chitosan and cellulose. The efficacy of this seed coating approach was tested against various soil- and seed-borne pathogens in oilseed crops, including soybean, groundnut, and safflower. Results indicate that safflower treated with the biopolymer chitosan-based T. harzianum Th4d 1 % liquid formulation blend exhibited a higher seed yield of 793 kg/ha, seed germination of 84.7 %, and a significant reduction in wilt and root rot by 64.7 %. In groundnut crops, the seed coating led to a seed germination rate of 88.6 %, a 72 % reduction in root rot incidence, and a seed yield of 3040 kg/ha. Similarly, soybean crops treated with the biopolymer chitosan and T. harzianum Th4d displayed 83.4 % seed germination, a 70.9 % reduction in root rot, and a seed yield of 1239 kg/ha. Further on-farm evaluations demonstrated promising results, with the biopolymer chitosan-based T. harzianum Th4d 1 % liquid formulation blend seed treatment showing a high incremental cost-benefit ratio in safflower (1:4.5), soybean (1:2.5), and groundnut crops (1:3.3). This study underscores the potential of microbe-based seed coating as a sustainable and economically viable strategy for pest management in oilseed crops."

Biocompatibility and antimicrobial efficacy of silver-doped borosilicate bioactive glass for tissue engineering application

Borate bioactive glasses are an auspicious material for tissue engineering applications due to their enhanced dissolution rate, bioactivity, and capacity to integrate therapeutic ions. In this study, borate-based S49B4 bioactive glass doped with silver at mass fraction of 0.5, 1, and 3 wt% were studied for bioactivity, degradation, antibacterial, and cytocompatibility. Thermogravimetric analysis revealed that the bioactive glasses were thermally stable between 600 and 700 °C. Fourier transform infrared spectroscopy and X-ray diffraction confirmed the successful synthesis of an amorphous phase of the doped borosilicate bioactive glasses and the incorporation of silver ion crystals within the structure, as well as associated contributions from borate and silicate network formers in the borosilicate bioactive glass. Morphological evaluation revealed that the borosilicate bioactive glasses exhibit a uniform and spherical shape across all formulations, with the mean particle size varying from 65 to 76 nm. An in-vitro acellular bioactivity in simulated body fluid medium showed that increasing the silver content increased the degradation rate and pH. Besides, scanning electron microscopy and Energy dispersive X-ray spectroscopy analysis revealed an upsurge in apatite production on the BBGs' surfaces as well as incremental Calcium-Phosphate ratio values of 1.50, 1.65 and 1.70 as the silver content increases. The antibacterial effect was tested against Escherichia coli and Staphylococcus aureus, while cytocompatibility was tested against human gingival epithelial cells. Silver integration at 1 wt percent yielded the most promising outcomes, which were particularly bactericidal at 79.8 % for Escherichia coli and 93.41 % for Staphylococcus aureus. Similarly, its Lactate Dehydrogenase percentage is significantly similar to the negative control employed in the study, indicating its biocompatibility. In contrast, 3 wt% silver exhibited the maximum bactericidal activity while also exhibiting mild cytotoxicity. In summary, our research indicates that elevated silver concentration enhances the bioactivity and antimicrobial characteristics of borosilicate bioactive glasses; nevertheless, a higher silver weight percent in this study also increases the possibility of cytotoxicity. It is therefore essential to carefully regulate the amount of silver doping at lower concentrations in order to maximize antibacterial action and minimize toxicity to human cells. The results presented here contribute to our understanding of the prospective use of silver doped borosilicate bioactive glasses as a possible material for tissue engineering applications.

Enhancing yield and economic benefits through sustainable pest management in Okra cultivation.

Okra (Abelmoschus esculentus) is a prominent vegetable crop in Asia, confronting persistent threats from pests such as leafhoppers, whiteflies, and shoot and fruit borers. Conventional chemical control methods, despite their adverse ecological effects, remain the primary approach for pest management. Indiscriminate chemical use has led to reduced biodiversity among natural predators and the disruption of food webs in ecosystems. To address these challenges, this study assessed the efficacy of integrated (IM) and biointensive (BM) pest management modules in comparison to conventional chemical methods (CM) for mitigating insect damage to okra leaves and fruits, and subsequently, their impact on okra yield. Our result revealed that the BM exhibited the least effectiveness but outperformed untreated control plots significantly. In contrast, both IM and CM significantly reduced damage from sap-sucking insects and borer pests. Notably, plots treated with the chemical module found decreased populations of natural enemies. The IM demonstrated the lowest fruit infestation rate (5.06%), yielding the highest crop production (8.97 t ha-1), along with the maximum net return (Indian Rupees: 44,245) and incremental cost-benefit ratio (3.31). Thus, the study suggested that the implementation of integrated pest management practices can result in higher okra yields and greater economic benefits. These findings shed light on the potential of sustainable agricultural practices as a safer and more economically viable alternative to chemical-intensive pest control in okra cultivation.

Response characteristics of soil slope under mainshock-aftershock sequences-type ground motions: Incremental damage effect, polarity effect, and correlation

Aftershocks frequently induce further damage to slopes that have already been compromised by mainshocks. Most of the current research concentrates on the case-based studies of structural response to the mainshock-aftershock sequences (MAS), however, the influence of the MAS parameter characteristics has not been adequately considered. In this study, the peak characteristic, spectrum characteristic, cumulative characteristic and polarity effect of the MAS were considered, the correlation between 21 MAS parameters and slope response were analyzed, and the response characteristics of soil slope under the MAS action were comprehensively and systematically revealed. The results show that: (1) Aftershocks can induce significant incremental damage to slopes, with the extent of this damage being contingent upon the severity of damage caused by the mainshock; (2) Among the MAS parameters, the Cumulative Absolute Velocity (CAVma) and Peak Ground Velocity (PGVma) are optimal for assessing the response of soil slopes under MAS conditions. Furthermore, the incremental damage caused by aftershocks can be predicted using the displacement increment ratio (δD); (3) The polarity of the MAS has an impact on the displacement of the slope, following the pattern: MAS along-slope direction > mainshock along-slope direction and aftershock reverse-slope direction > aftershock along-slope direction and mainshock reverse-slope direction > MAS reverse-slope direction; (4) The MAS polarity also affects the correlation between the MAS parameters and the slope displacement response, especially for the aftershock displacement. The research results aim to provide a foundation for the selection of evaluation factors and the analysis of soil slopes stability under the MAS action.

Finite element method (FEM) analysis of heat transfer by natural convection in a circular cavity containing a corrugated hollow cylinder

PurposeThe purpose of this study is to analyze the free convection phenomena arising from a temperature disparity between a cold circular cylinder and a heated corrugated cylinder.Design/methodology/approachNumerical simulations were used to analyze the convection patterns. The inner cylinder, made of a thermally conductive solid material, was heated through its inner surface, while the space between the cylinders was filled with air. The governing equations for velocity, pressure and temperature were solved using a Galerkin finite element method-based solver for partial differential equations.FindingsThe study explored various parameters affecting the dynamic and thermal structure of the flow, including the Rayleigh number (103 ≤ Ra ≤ 106), the number of corrugations of the inner cylinder (3 ≤ N ≤ 18), the thermal conductivity of the hollow cylinder (1 ≤ K ≤ 200) and the angle of inclination of the inner cylinder (0° ≤ φ ≤ 90°). Results indicated a notable sensitivity of flow intensity to changes in the Rayleigh number and the inner cylinder’s inclination angle φ. Particularly, for Ra = 106, the average heat transfer rate increased by 203% with a K ratio increment from 1 to 100 but decreased by 16.3% as the number of corrugations increased from 3 to 18.Originality/valueThis research contributes to understanding the complex interplay between geometry, thermal properties and flow dynamics in natural convection systems involving cylindrical geometries. The findings offer useful insights for improving the transfer of heat procedures in real-world situations.

The footprint mismatch of cervical disc arthroplasty comes from degenerative factor besides ethnic factor

A mismatch in footprints of cervical total disc arthroplasty (CTDA) implants occasionally occurred in Asian population and it had been attributed solely to ethnic factor. Yet, cervical degeneration process may play a role. Our purpose was to compare the cervical vertebra morphometric data with and without degeneration. The study included patients with CT scans of cervical spine from our hospital between January, 2019, and September, 2021. The total cervical degenerative index (TCDI) of each patient were collected by adding CDI score for 5 disc-levels. Patients were categorized into normal (TCDI 0–5) and degeneration groups (TCDI 6–60). Various measurements of the C3–C7 vertebral body and endplate were taken. Forty-nine patients in the normal group and 55 in the degeneration group were included. No significant difference was noted in gender, BH, BW, or BMI except age and TCDI (p < .001). During degeneration, disproportional endplate size changes were observed, with an increment ratio of 12–20% in the anteroposterior and 5–17% in the mediolateral plane throughout C3–C7, while vertebral body height remained constant. In conclusion, degeneration process, besides ethnic factor, causes the endplate size and shape mismatch. This information can help spine surgeon choose appropriate implants in CTDA surgery.

Effects of different forging ratios on microstructure, mechanical properties and friction and wear behaviour of Q355D steel

ABSTRACT To meet the mechanical performance demands of large oil cylinder barrels and piston rods, an investigation into the forging process of Q355D low-alloy high-strength steel was conducted. This study rigorously examined the impact of various forging ratios (2.6, 4.3, and 6.2) on both the microstructural characteristics and mechanical properties (including tensile strength, hardness, elongation, and impact toughness) of Q355D steel through a comprehensive systematic analysis. The findings revealed that an escalation in the forging ratio resulted in a refinement of the grain size in both ferrite and pearlite, concurrently with the fragmentation of MnS inclusions. The augmentation in both hardness and impact toughness correlated proportionally with the increased forging ratio, primarily attributed to the grain size refinement and augmented dislocation density. The yield strength exhibited a corresponding increase with the forging ratio increments: 303.56 MPa, 324.32 MPa, and 346.69 MPa, while hardness values were recorded as 174.32 HV, 187.31 HV, and 200.95 HV, respectively. Furthermore, grain refinement significantly contributed to enhancing the steel's ductility and toughness. Additionally, the escalated forging ratio notably reduced the duration required for the steel to reach a stabilized friction coefficient and reduced the stabilized friction coefficient, consequently amplifying the steel's resistance to frictional wear.

Comparison of the Cost Effectiveness of Orthognathic Surgery Treatment Between Orthodontic-first and Surgery-first Approaches in the Surgical Phase in Thammasat University Hospital

Objectives: This retrospective cohort study aims to evaluate the costs and advantages of the surgical phase of the surgery-first approach (SFA) versus the orthodontic-first approach (OFA). Methods: Orthognathic surgery has been described as having two concepts: an orthodontic-first approach and a surgery-first approach. However, there was no consensus on which group has the best cost effectiveness in the surgical treatment phase. In total, 70 patients were treated; half of the patients were treated via the SFA, and another half were treated via the OFA. The information collected included operation cost, operation time, total hospital cost, and length of hospitalization. Effectiveness was determined by quality of life, which was measured with the Orthognathic Quality of Life Questionnaire Thai Version (OQLQ) before and then 6 months after treatment. The cost effectiveness was assessed with an incremental cost effectiveness ratio (ICER) and an incremental time effectiveness ratio (ITER). Results: The results indicated the intervention cost and time of SFA were slightly higher but more effective than those of OFA. However, the operation cost (p=0.375), operation time (p=0.556), total hospital cost (p=0.363), and length of hospitalization (p=0.643) and OQLQ scores (p=0.344) of both groups were not significantly different. Conclusions: The intervention cost and time of SFA were slightly higher but more effective than those of OFA. Depending on the result of this study, SFA treatment planning was a good choice for orthognathic treatment.

Several common methods of making vesicles (except an emulsion method) capture intended lipid ratios

Researchers choose different methods of making giant unilamellar vesicles to satisfy different constraints of their experimental designs. A challenge that arises when researchers use a variety of methods is that each method may produce vesicles with a different average lipid ratio, even if all experiments use lipids from a common stock mixture. Here, we use mass spectrometry to investigate ratios of lipids in vesicle solutions made by five common methods: electroformation on indium tin oxide slides, electroformation on platinum wires, gentle hydration, emulsion transfer, and extrusion. We made vesicles from either five-component or binary mixtures of lipids chosen to span a wide range of physical properties: di(18:1)PC, di(16:0)PC, di(18:1)PG, di(12:0)PE, and cholesterol. For a mixture of all five of these lipids, ITO electroformation, Pt electroformation, gentle hydration, and extrusion methods result in only minor shifts in lipid ratios (≤5 mol %) relative to a common stock solution. In contrast, emulsion transfer results in ∼80% less cholesterol than expected from the stock solution, which is counterbalanced by a surprising overabundance of saturated PC-lipid relative to all other phospholipids. Experiments using binary mixtures of saturated and unsaturated PC-lipids and cholesterol largely support results from the five-component mixture. In general, our results imply that experiments that increment lipid ratios in small steps will produce data that are highly sensitive to the technique used and to sample-to-sample variations. For example, sample-to-sample variations are ∼±2 mol % for five-component vesicles produced by a single technique. In contrast, experiments that explore larger increments in lipid ratio or that seek to explain general trends and new phenomena will be less sensitive to sample-to-sample variation and the method used.