The bolted joint is an important link that affects the accuracy maintenance of the heavy-duty machine tool. Due to bolts of the crossbeam are under a preload of several hundred kN for an extended period, the bolt gradually creeps and elongates as the machine tool's service time increases. This relaxation of the preload results in a degradation of the bolted stiffness, weakening the dynamic response of the bolted joint system. To reveal that the degradation law of crossbeam bolting performance of the heavy-duty machine tool induced by bolt creep, based on the Norton-Bailey(NB) model, a method for analyzing the creep of bolts with precision threads is proposed, and the influence of thread geometry and friction coefficient on the creep stress relaxation of bolts is studied. Considering the interface contact, an interface contact stiffness model is derived based on fractal theory. The dynamic equation of the bolted beam system is established using the Matrix27 stiffness matrix. The dynamic response of the bolted system, resulting from the degradation of interface stiffness due to bolt creep, is analyzed. The results show that the stress relaxation of bolt creep is not only related to creep parameters, but also related to the mate-thread interaction. The dynamic model with Matrix27 stiffness matrix is comparable to experiment in calculation accuracy and efficiency, which provides technical guidance for machine tool dynamics analysis and effectively solves the scientific problem of beam bolting performance degradation during the service of heavy-duty machine tools.