The Yamuna River A tributary of the Ganga river is one of India's most well-known and important rivers. Regrettably, numerous sections of the Yamuna River are heavily polluted, including many metropolitan areas like Prayagraj, Agra, Delhi etc. Water pollution with lead is a long-term problem. Rural and urban expansion, as well as rapid industrial development, are key sources of lead poisoning in lakes, rivers, groundwater, and other water sources as a result of increased population growth. Water samples were taken from each of the five sampling locations. Samples were taken throughout the course of a whole 2019 year, from January to December, with a 20–25-day break between them, with climate variation as a primary factor. Water sample were analysed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and Atomic Absorption Spectroscopy (AAS) were used to quantify the content of lead (Pb) in water from the Yamuna in Delhi. When results were compared to the WHO permissible limits of Lead (Pb) and Nickel (Ni) in water, they were found to be greater. It can be observed that the concentration increases as the temperature rises and the humidity falls. Lead and nickel are both very poisonous in nature, and human and animal exposure to these heavy metals through water can cause chronic intoxication, which can be fatal.