Increase In Wet Weight Research Articles

High-Density Fermentation of Lactobacillus plantarum P6: Enhancing Cell Viability via Sodium Alginate Enrichment

Lactobacillus plantarum exhibits a wide range of beneficial physiological functions, including maintaining intestinal microbiota balance, reducing serum cholesterol, and promoting digestive health. According to the specific nutrient requirements of Lactobacillus plantarum P6, we investigated the effects of various carbon sources, nitrogen sources, trace elements, growth-promoting substances, as well as the initial pH and inoculum size on the growth of Lactobacillus plantarum P6 under fermentation conditions. The optimal growth conditions for Lactobacillus plantarum P6 were identified to facilitate high-density fermentation in small-scale fermenter production, achieving a cell concentration of 1.03 × 1011 CFU/mL. This resulted in a 2.5-fold increase in bacterial wet weight, and fermentation time was reduced to 12 h when utilizing a specific medium enriched with 0.2% sodium alginate. It is hypothesized that sodium alginate forms a protective film around the bacterial cells, promoting cell aggregation and enhancing self-coalescence, potentially triggering a bacterial community effect. These results provide a basis for the industrial-scale high-density cultivation of Lactobacillus plantarum, offering potential for enhanced biotechnological applications.

Impact of dielectric barrier discharge plasma and plasma-activated water on cotton seed germination and seedling growth

Unfavorable environmental conditions during planting can reduce seed germination and hinder seedling growth. To address this issue, manufacturers are exploring innovative and cost-effective methods, such as cold plasma discharge. This simple, low-cost, and efficient physical technique induces significant biological responses in seeds and plants without the use of traditional, environmentally hazardous chemicals. This study investigated the impact of dielectric barrier discharge (DBD) plasma and plasma-activated water (PAW), produced by gliding arc plasma, on the germination and seedling growth of My344 cotton seeds. The seeds were pre-treated with 80 W of DBD plasma for 0, 1, 2, and 3 min, and subsequently soaked for 90 min in PAW with varying pH levels of 5.82, 3.88, 3.63, and 3.38. The results showed that plasma treatment positively influenced seed germination and seedling growth. The highest germination percentage (98.89 %) was observed with 1 min of DBD treatment, followed by PAW priming at pH levels of 3.63 and 3.38. Additionally, a 3-min DBD treatment followed by soaking in PAW with a pH of 3.63 led to significant increases in stem length (76.76 %), root length (48.77 %), and wet weight (76.44 %). Furthermore, it was observed that the electrical conductivity of the seeds in all groups decreased significantly with increased PAW acidity. The physical and chemical effects of cold DBD plasma on the seed surface, as well as changes in hydrophilicity, were further examined using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and water contact angle imaging.

Study of the phytotoxicity of margines on Pistia stratiotes L.

Abstract The olive trituration activity is one of the most important industrial activities in Fez, Morocco. These effluents are highly loaded with organic, inorganic, and phenolic compounds without any preliminary treatment that affects water quality. In this sense, the waters of Oued Fez are deteriorating due to the discharge of wastewater from oil mills and the excessive proliferation of Pistia stratiotes L., an invasive macrophyte that represents a significant stress to the aquatic ecosystem, eutrophication, and a reduction in biodiversity. This ecological situation has prompted us to carry out phytotoxicity bioassays based on the detection of the lethal concentration of P. stratiotes in the wastewater from the olive oil mill to evaluate the sensitivity and tolerance of macrophytes to the different concentrations of total polyphenols (TPP) present in the olive mill wastewater. To estimate their impact on the environment. Fresh whole plants of P. stratiotes were exposed to varying concentrations of olive oil mill wastewater with a series of TPP concentrations (0–30 mg/l) for 1 week in the natural environment. The results also show that P. stratiotes is able to grow rapidly in culture with 20 mg/l of TPP; the highest growth of wet weight of P. stratiotes occurred at 20 mg/l treatment with an average of 13 g wet weight increase. At 0 mg/l, treatment is known to cause very slow growth with an average of 2 g. Olive oil mill wastewater was toxic to the plant at concentrations higher than 30 mg/l, and the phytotoxic effect was manifested by retardation of growth, detachment of roots, wilting, and chlorosis of leaves. This indicates that polyphenols have great potential to inhibit the proliferation of P. stratiotes in aquatic environments.

Effect of the number of Cyperus rotundus and medium height on the performance of batch-constructed wetland in treating aquaculture effluent

Increasing aquaculture cultivation produces large quantities of wastewater. If not handled properly, it can have negative impacts on the environment. Constructed wetlands (CWs) are one of the phytoremediation methods that can be applied to treat aquaculture effluent. This research was aimed at determining the performance of Cyperus rotundus in removing COD, BOD, TSS, turbidity, ammonia, nitrate, nitrite, and phosphate from the batch CW system. Treatment was carried out for 30 days with variations in the number of plants (10, 15, and 20) and variations in media height (10, 12, and 14 cm). The result showed that aquaculture effluent contains high levels of organic compounds and nutrients, and C. rotundus can grow and thrive in 100% of aquaculture effluent. Besides that, the use of C. rotundus in CWs with the effect of numbers of plants and media height showed performance of COD, BOD, TSS, turbidity, ammonia, nitrate, nitrite, and phosphate with 70, 79, 90, 96, 64, 82, 92, and 48% of removal efficacy, respectively. There was no negative impact observed on C. rotundus growth after exposure to aquaculture effluent, as indicated by the increase in wet weight, dry weight, and growth rate when compared to the control. Thus, adding aquaculture effluent to CWs planted with C. rotundus supports the growth and development of plants while also performing phytoremediation.

Obtaining a primary suspension cell culture of Dracocephalum palmatum Stephan ex Willd

Dracocephalum palmatum Steph. grows on the southern slopes of the Oymyakon plateau in Yakutia (Northeast of Russian Federation) in conditions of harsh continental climate with continuous permafrost. The aboveground phytomass of the plant contains various complexes of secondary metabolites including polyphenolic compounds. It is a potential source of secondary metabolites needed for practical use in the pharmaceutical industry. The aim of the study is to obtain a primary suspension cell culture of Dracocephalum palmatum, growing in the conditions of the Cold Pole — Oymyakon. The work includes optimization of the nutrient medium for introducing calluses into a suspension culture, analysis of the dynamics of biomass growth of the obtained suspension culture, and morphological characteristics of the cells of the suspension culture. The callus cell cultures of Dracocephalum palmatum, cultivated on Murashige-Skoog (MS) medium with the addition of 0.5 mg/L α- naphthylacetic acid (NAA) and 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), were most successfully transitioned into suspension culture. The maximum biomass growth of cell suspension culture was observed when cultivated in liquid MS medium with the addition of 2,4-D (0.5 mg/L), 6- benzylaminopurine (0.5 mg/L), and NAA (0.5 mg/L). The primary cell suspension culture of Dracocephalum palmatum, cultivated for 22 days, had an increase in wet weight of 9,2084 g, dry weight — 0,34135 g, and contained dedifferentiated aggregates of parenchyma-like cells and single round-shaped cells. Samples of the obtained cell suspension culture of Dracocephalum palmatum will be used for the analysis of secondary metabolites and for the development of optimal cultivation conditions in a bioreactor.

Seasonal temperature variation in Zostera marina seedlings under ocean acidification

ObjectiveTo investigate the responses of Zostera marina seedlings to the individual and combined stresses of seasonal temperature increase and ocean acidification (OA) caused by global climate change and anthropogenic factors. This data will help in efforts to protect and restore seagrass beds in temperate coastal zones of China.MethodsA mesoscale experimental system was utilized to analyze stress response mechanisms at multiple levels - phenotype, transcriptome, and metabolome - during the seedling stage of Z. marina, a dominant temperate seagrass species in China. The study monitored the seedlings under varying conditions: increased seasonal temperature, OA, and a combination of both.ResultsFindings revealed that under high-temperature conditions, carotenoid biosynthesis was stimulated through the upregulation of specific metabolites and enzymes. Similarly, the biosynthesis of certain alkaloids was promoted alongside modifications in starch, sucrose, and nitrogen metabolism, which improved the plant’s adaptation to OA. Unique metabolic pathways were activated under OA, including the degradation of certain amino acids and modifications in the citric acid cycle and pyruvate metabolism. When subjected to both temperature and OA stresses, seedlings actively mobilized various biosynthetic pathways to enhance adaptability and resilience, with distinct metabolic pathways enhancing the plant’s response under diversified stress conditions. In terms of growth, all treatment groups exhibited significant leaf length increase (p < 0.05), but the weakest growth index was observed under combined stress, followed by the thermal treatment group. Conversely, growth under OA treatment was better, showing a significant increase in wet weight, leaf length, and leaf width (p < 0.05).ConclusionSeasonal temperature increase was found to inhibit the growth of Z. marina seedlings to some extent, while OA facilitated their growth. However, the positive effects of OA did not mitigate the damage caused by increased seasonal temperature under combined stress due to seedlings’ sensitivity at this stage. Our findings elucidate differing plant coping strategies under varied stress conditions, contingent on the initial environment. This research anticipates providing significant data support for the adaptation of Z. marina seedlings to seasonal temperature fluctuations and global oceanic events like OA, propelling the effective conservation of seagrass beds.

Screening and characterization of plant growth promoting rhizobacteria and their effect on growth and yield of barley (Hordeum vulgare L.)

A multitude of roles are played by microbes in food and agriculture that include nutrient cycling and management, organic matter decomposition and fermentation. Plant growth promoting rhizobacteria (PGPR), representing microbial groups and with the ability of colonizing plant roots, influence plant growth through various indirect and direct modes in order to promote its growth and/or protect it from diseases or damage due to insect attack. This research was conducted during August 2022 to April 2023 at Chandigarh University, Punjab, wherein 15 bacterial isolates were isolated from rhizospheric soils of different crop plants. Isolates were identified based on biochemical characteristics and their plant growth promotional efficiencies were tested in-vitro. A total of ten isolates were found positive for four or more than 4 plant growth promoting traits. These isolates were further identified at molecular level using amplification and sequencing of 16S rDNA sequences. Sequences were submitted to the NCBI GenBank portal and accessions numbers were obtained. Potential microbial strains were inoculated with Barley seeds and a significant increase in plant height, dry weight and wet weight as well as number of seeds was recorded in inoculated plants as compared to control plants. However, maximum plant growth and yield was recorded in the plants inoculated with isolates 21B (Klebsiella quasivariicola) and 28B (Arthrobacter sp.). These isolates may become very crucial alternatives of chemical fertilizers for sustainable agriculture.

The influence of various tillage technologies on soil fertility, weed infestation and crop productivity in crop rotation

As a result of research for the third rotation of crop rotation (2008-2015), conducted on the basis of the ISA - a branch of the Federal State Budgetary Scientific Institution FNAC VIM in the conditions of the Ryazan region, the influence of various tillage technologies on the fertility of podzolized chernozem, weediness of crops and productivity of crop rotation crops was established. The smallest number of weeds was observed in the variants with combined treatments - up to 11.2-12.9 pcs/m? against the background of the use of herbicides and 21.3-19.6 pcs/m? against the unprotected background, which is lower compared to the control by 19.4-7.2 % in variants with protection and by 28.3-34.0 % in variants without protection. Also in these variants, a decrease in the wet weight of weeds was observed - by 13.5-15.2 % against the background with herbicides, respectively, while against the background without protection, an increase in wet weight was observed by 21.6-11.8 %, respectively. With flat-cut tillage, an increase in weed infestation to 16.2 pcs/m? with herbicides and 37.9 pcs/m? on an untreated background, as well as a wet weight of weeds up to 110.4-108.2 g/m?, respectively, was noted. The maximum amount of digestible forms of nitrate nitrogen is observed when using deep combined treatments up to 7.5-8.1 mg/kg of soil. The greatest increase in the content of humus in the soil layer of 0-30 cm was noted according to the results of the use of 2-tier tillage – up to 3.99 %, which exceeds the initial value by 0.38 %. The highest yield was obtained when using different-depth treatments – 5.19 t/ha without protection and 5.45-5.52 t/ha with the use of herbicides. The maximum productivity of grain crops was also obtained in variants with combined treatments and amounts to 5.93 thousand feed units/ha against the background without protection, and 6.50 thousand feed units/ha against the background of chemicalization.

Increasing mustard (Brassica juncea L.) yields through exposure sound and preventive pest management based on refugia plants

Abstract. In Indonesia, mustard plants will increase by 8.2% in 2021, but more than that is needed to meet the community's needs due to a growing population. Pests and fertilization problems hinder expected growth. The provision of plant fertilizers can be replaced through the application of sound. Exposure sounds to plants can increase plant growth by opening stomata, improving water absorption, and increasing plant nutrient uptake. This study aimed to examine the application of sound effects and refugia plants t890po increase the growth and yield of mustard greens (Brassica juncea L.). The study used a Time's Series Randomized Block Design with 3 replications. The first factor is sound applications (S) consisting of S0 = without sound, S1 = rock music (Frequency 21-14,000 Hz), and S2 = classical sound (Frequency 21-14,000 Hz). The second factor is the refugia plant (R) consisting of R0 = without refugia, R1 = Tagetes erecta L., R2 = Zinnia elegans L, and R3 = Ocimum bassilicum L. The application of classical sound frequency (20-14,000 Hz) affects the growth process of mustard plants through the increase in plant height (14.5%), leaf area (20.92%), wet weight (3.5%), dry weight (26.41%), total chlorophyll (11.62%) and plant N content (31.03%). Meanwhile, rock music frequency (20-14,000 Hz) can increase plant height (2.97%), leaf area (19.11%), and total stomata (9.6%). Refugia plants affect the process of plant protection through their ability to attract pests in the research area.

Phytoremediation Using Different Numbers of Individual Kayu Apu Plant (Pistia stratiotes) in Reducing Lead (Pb) Levels in Paper Recycling Factory Waste

Recycled paper mill waste contains inorganic materials, one of heavy metal lead. Metal lead is non-essential and non-degradable that harmful to living things. Reduce lead levels by using Kayu Apu plants (Pistia stratiotes) is needed. The aims, to determine number of Kayu Apu plants that effective in reducing lead levels, temperature and pH of optimum waste according to quality standards, increase in plant's wet weight, and morphological condition of plants. Method is experiment with completely randomized design. Treatment consisted of number of Kayu Apu plants A: control, B: 2 individuals, C: 5 individuals, and D: 9 individuals with 3 replications. Acclimatization of Kayu Apu plant was carried out, measuring physical, chemical and biological parameters, then data were analyzed using one-way ANOVA and paired sample t-test. Results showed that lead levels of treatment using 9 individuals decreased while in control, 2 individuals and 5 individuals increased. Average temperature and pH of waste are still optimum according to quality standards. Average fresh weight of plants increased in treatments B and C, while treatment D decreased. Number of Kayu Apu plants that effective in reducing lead was treatment using 9 individuals. Average temperature and pH of waste were optimum according to quality standards. Wet weight of plants increased in treatments B and C while D decreased. Morphology of Kayu Apu leaves in all treatments turned yellow, dried and fall off but the buds grow.Keywords: Kayu Apu (Pistia stratiotes), lead (Pb), Paper mil waste

Structure-Activity Relationships of Triphenylethylene Derivatives and Their Evaluation as Anticancer and Antiviral Agents.

Tamoxifen (TAM) is a selective estrogen receptor modulator (SERM) that is used in the treatment of breast cancer, yet with the risk of developing uterine cancer. A perfect SERM would act as an estrogen activator on bones, the cardiovascular system, and the central nervous system while providing neutral or estrogen blocking effects on the breast and the uterus. Herein, we report on the design, synthesis, and evaluation of new rigid and flexible TAM analogues. Mainly, a chloro substituent is introduced at the para position of the TAM ring C blocking the CYP2D6 hydroxylation site. Most compounds showed estrogenic activity higher than TAM using the yeast estrogen screen assays, indicating the determinant role of the chloro substituent upon functional activity. Despite being estrogenic, compound 2B showed potent antiproliferative activity in the NCI 60 cell lines with mean GI50 = 3.67 μM, GI50 = 1.05 μM on MCF-7 cell lines, and GI50 = 1.30 μM on MDA-MB-231. The estrogenic activity of compound 2B was further confirmed by stimulating alkaline phosphatase in Ishikawa cells, and it showed no increase in relative uterine wet weight in ovariectomized rats. Compound 2F showed EC90 = 0.31 μg/mL and SI90 = 60 against Ebola virus; this is 200-fold more potent than the positive control favipiravir. This is the first time to report estrogenic triphenylethylenes as anti-EBOV agents. The anti-EBOV activity reported is a function of the substitution pattern of the scaffold rather than the functional activity. Moreover, compound 3D showed excellent PO pharmacokinetic properties in mice. In conclusion, for this class of TAM-like compounds, the blockage of the p-position of ring C is decisive for the functional activity; meanwhile, the triarylethylene substitution pattern is detrimental for the antiviral activity.

Konsentrasi Dan Lama Perendaman Ekstrak Bawang Merah (Allium Cepa L.) Terhadap Invigorasi Benih Jagung ( Zea Mays L.) Kadarluasa

Seeds that are expired will result in delays in the process of growth and germination. This study aims to determine the concentration of shallot extract on the growth of expired corn seeds, determine the soaking time of shallot extract on the growth of expired corn seeds and determine the best combination between the concentration and soaking time of expired shallot extract on the growth of expired corn seeds. This research was carried out in the Pat Petulai University laboratory from April 2021 to May 2021 in Curup. The seeds used are hybrid corn seeds Pioneer P32 which had expired on April 15, 2020. This study used a completely randomized design (CRD) factorial with 2 factors. The first factor is the concentration of shallot extract (K) consisting of 4 levels K0 = Without giving shallot extract (Control), K1 = Concentration of shallot extract 20% /100 ml distilled water, K2 = Concentration of shallot extract 40% /100 ml distilled water .K3 = Concentration of shallot extract 60%/ 100 ml of distilled water, while the second factor is soaking time (P) consisting of 4 levels: P0 = Without immersion (control) P1 = 3 hours P2 = 6 hours P3 = 9 hours. The observed variables included germination rate, sprout height, stem diameter, plant fresh weight. Data were analyzed using Analysis of Variance at 5% level. If there is a significant difference, it will be continued with the Least Significant Difference (LSD) test. The results showed that the treatment with shallot extract concentration and soaking time had a significant effect on plant height, stem diameter and plant fresh weight, but had no effect on sprout height and root length. It was found that there was an interaction between the concentration of shallot extract and the soaking time because it could increase the increase in plant height and plant wet weight. The best plant height, stem diameter and plant fresh weight were seen in the treatment of shallot extract concentration of 60%/100 m l of distilled water.
 

Anti-Inflammatory, Antinociceptive, Antipyretic, and Gastroprotective Effects of Eurycoma longifolia Jack Ethanolic Extract.

Tongkat ali (Eurycoma longifolia Jack) (ELJ) is a plant in the Simaroubaceae family. Its roots are used in traditional Thai medicine to treat inflammation, pain, and fever; however, the antiulcer abilities of its ethanolic extract have not been studied. This study examined the anti-inflammatory, antinociceptive, antipyretic, and gastroprotective effects of ethanolic ELJ extract in animal models and found that ELJ effectively reduced EPP-induced ear edema in a dose-dependent manner and that a high dose of ELJ inhibited carrageenan-induced hind paw edema formation. In cotton-pellet-induced granuloma formation, a high dose of ELJ suppressed the increases in wet granuloma weight but not dry or transudative weight. In the formalin-induced nociception study, ELJ had a significant dose-dependent inhibitory impact. Additionally, the study found that yeast-induced hyperthermia could be significantly reduced by antipyretic action at the highest dose of ELJ. In all the gastric ulcer models induced by chemical substances or physical activity, ELJ extracts at 150, 300, and 600 mg/kg also effectively prevented gastric ulcer formation. In the pyloric ligation model, however, the effects of ELJ extract on gastric volume, gastric pH, and total acidity were statistically insignificant. These findings support the current widespread use of Eurycoma longifolia Jack in traditional medicine, suggest the plant's medicinal potential for development of phytomedicines with anti-inflammatory, antinociceptive, and antipyretic properties, and support its use in the treatment of gastric ulcers due to its gastroprotective properties.

Overexpression of GRK2 in vascular smooth muscle leads to inappropriate hypertension and acute heart failure as in clinical scenario 1

Clinical scenario 1 (CS1) is acute heart failure (HF) characterized by transient systolic blood pressure (SBP) elevation and pulmonary congestion. Although it is managed by vasodilators, the molecular mechanism remains unclear. The sympathetic nervous system plays a key role in HF, and desensitization of cardiac β-adrenergic receptor (AR) signaling due to G protein-coupled receptor kinase 2 (GRK2) upregulation is known. However, vascular β-AR signaling that regulates cardiac afterload remains unelucidated in HF. We hypothesized that upregulation of vascular GRK2 leads to pathological conditions similar to CS1. GRK2 was overexpressed in vascular smooth muscle (VSM) of normal adult male mice by peritoneally injected adeno-associated viral vectors driven by the myosin heavy chain 11 promoter. Upregulation of GRK2 in VSM of GRK2 overexpressing mice augmented the absolute increase in SBP (+ 22.5 ± 4.3 mmHg vs. + 36.0 ± 4.0 mmHg, P < 0.01) and lung wet weight (4.28 ± 0.05 mg/g vs. 4.76 ± 0.15 mg/g, P < 0.01) by epinephrine as compared to those in control mice. Additionally, the expression of brain natriuretic peptide mRNA was doubled in GRK2 overexpressing mice as compared to that in control mice (P < 0.05). These findings were similar to CS1. GRK2 overexpression in VSM may cause inappropriate hypertension and HF, as in CS1.

Potassium Application Enhanced Plant Growth, Mineral Composition, Proximate and Phytochemical Content in Trachyandra divaricata Kunth (Sandkool).

Wild leafy vegetables are commonly included in the diet of people in rural homesteads. Among various wild edible vegetables in South Africa, Trachyandra divaricata (Sandkool) is one of the most abundant but underutilized due to the dearth of literature on its cultivation and nutritional value. In the present study, the effect of potassium application and pruning on growth dynamics, mineral composition, and proximate and phytochemical content in T.&amp;nbsp;divaricata were evaluated. Treatments consisted of three potassium concentrations (0.0072, 0.0144, and 0.0216 M) supplemented in the form of potassium sulphate (K2SO4) with four pruning levels (unpruned, 5, 10, and 15 cm) applied in each treatment. The potassium doses were added to the nutrient solution, while the control treatment was sustained and irrigated with nutrient solution only. The results revealed a significant increase in flower bud yield, height, total dry and wet weight of shoots and roots, as well as ash and neutral detergent fibre in plants irrigated with 0.0072 M of K2SO4 without pruning. Conversely, chlorophyll content and Ca were comparable among treatments, while the highest yield of Na, P, N, and Zn was recorded in treatment 100 mL of K2SO4 with 10 cm pruning. Likewise, the highest antioxidant value (Polyphenols, Flavonol and DPPH) was obtained from plants irrigated with 0.0072 M of K2SO4 with 10 cm pruning. Based on these findings, T. divaricata is a promising leafy vegetable as a minimum dose (0.0072 M) of K with moderate pruning optimised its productivity in terms of growth, biomass parameters, nutritional content, and antioxidant potential. Due to its rich nutritional value, the plant should be domesticated and studied further for its potential nutraceutical benefits.

Pengaruh Pemberian Biourin Sapi terhadap Pertumbuhan dan Produksi Tanaman Kangkung Darat (Ipomoea reptans Poir) pada Tanah Ultisol

Ultisols have the potential to be developed as productive agricultural land, but need several efforts by means of organic matter and fertilizer applications. Biourine is a fermented liquid waste from cow urine. One of the commodities developed in Ultisol is spinach (Ipomoea reptans Poir). The study aims to determine the effect of treatments of six biourin concentrations on available nitrogen, soil pH, and growth plants of spinach on Ultisol soil. The single factor of completely randomized design (CRD) used as an experimental design consists of six treatments with four replications (24 experimental units). The results showed that biourine had no effect on increasing soil ammonium-N and had no effect on soil nitrate-N and soil pH. Biourin affects the increase in wet weight and dry weight of spinach plants. There is a relationship between soil NO3- and NH4+ levels with wet weight. Biourin concentration level of 2% is the best concentration to increase ammonium-N, wet weight and dry weight.

Evaluation of Anti Estrogenic Activity and Anti-Osteoporotic Activity of Extracted Quercetin from Bambusa arundinacea Leaves on Ovariectomized Rats

Phytoestrogens has now become a emerging era of interest for the researchers. The mimicking effects of phytoestrogens had lead to its wide use in reproductive system. In this study it was aimed to study the estrogenic activity and anti-osteoporotic activity of isolated quercetin female Wistar rats. The estrogenic effect was analyzed by uterotropic assay, vaginal cytology and measurement of vaginal opening in female Wistar rats. The administration of isolated quercetin in ovariectomized immature and mature female Wistar rats in a dose of 30 mg/kg b.w. resulted in significant increase in the uterine wet weight when compared with ovariectomized control rats. The treated rats, showing only Cornified epithelial cells was an indication of the presence of the estrogen and also showed 100% vaginal opening. Thirty six female albino Wistar rats were randomly divided into six groups (n=6). After 60 days of Ovariectomy, animals were treated with of isolated quercetin for the next 45 days. Finally femur bone length, weight, bone ash calcium level, and bone mineral density (BMD) were estimated. The levels of serum alkaline phosphates (ALP), calcium, and phosphorous, and bone histopathology were also evaluated. OVX-induced increased serum ALP, calcium, and phosphorous levels were impaired in of isolated quercetin treated rats. The isolated quercetin which was evident by uterotropic assay, measurement of vaginal opening, and histopathological changes. It also exhibited a significant anti-osteoporotic effect in the experimental model of OVX-induced osteoporosis in rats, indicating advantageous effect in postmenopausal osteoporosis. Thus it concludes the Anti Estrogenic activity and Anti-Osteoporotic activity of extracted Quercetin from Bambusa arundinacea leaves on ovariectomized rats.

Effect of 50-Hertz Sinusoidal Vibration on the Uterus in Ovariectomy-Induced Osteoporotic Rats.

Objective To evaluate the influence of sinusoidal vibration (50-Hertz) stimulation on the uterus of osteoporotic rats. Methods We constructed an osteoporosis rat model by ovariectomy (OVX). 36 3-month-old Sprague Dawley rats were randomly divided into the control group, vibrating group, sham operation group, sham operation vibrating group, OVX group, and OVX vibrating group (n = 6 per group). Rats started to vibrate one week after the operation: one 10 minutes 50-Hertz sinusoidal vibration per day, except for Saturday and Sunday. In the second, 8, and 12 week after vibration stimulation, rats were sacrificed in batches. And then, the uteruses were taken out to measure the wet weight and calculate uterus relative wet weight. Results Compared with the control group, OVA induced a significant increase in wet weight and relative wet weight in rat uterus. The vibration was to the uterus wet weight and the uterus relative wet weight in ovariectomized rats and at the same time had no significant effect, but the 12-week prolonged vibration can significantly reduce the uterus wet weight and the uterus relative wet weight in ovariectomized rats than 2 weeks. Conclusions The uterus wet weight and the uterus relative wet weight were increased in the OVA-induced osteoporosis rats. The 50-Hertz sinusoidal vibration had no significant effect on the uterus wet weight and the uterus relative wet weight in the ovariectomized rats at the same time, but 12 weeks of vibration can significantly reduce the uterine wet weight and uterine relative wet weight of ovariectomized rats. And the uterus relative wet weight can be used as a new indicator of stimulating the uterus.

Plant Growth Promotion and Biocontrol Properties of Aneurinibacillus migulanus Isolated from Maize Roots (Zea mays)

Aims: To isolate Plant Growth Promoting Bacillus strain from maize roots, to evaluate its biocontrol potentials and to characterize the isolate using16S rRNA sequencing.&#x0D; Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, between February 2019 and March 2020.&#x0D; Methodology: The isolation of Plant Growth Promoting Rhizobacteria (PGPR) from maize roots was done using Pikovskaya (PVK) agar. Quantitative determination of phosphate was carried out using PVK broth. Evaluations of other plant growth promoting properties were carried out such as IAA, etc. Fusarium and Enterobacter plant pathogens were isolated from diseased maize plants. The in vitro antagonism effects of the PGPR isolates against the pathogens were analyzed using the dual culture plate technique. The pot experiment was carried out in a completely randomized design. Plant characteristics such as plant height, shoot and root weight, chlorophyll content, as well as disease assessment were recorded accordingly. The organisms were identified using phenotypic and molecular methods.&#x0D; Results: Seven PGPR bacteria were isolated from maize (Zea mays) roots using PVK agar. Aneurinibacillus migulanus gave the highest solubilization index of 4.21 while isolate IS48 gave the lowest solubilization index of 1.47. A. migulanus produced IAA, ammonia and cellulase enzyme but no hydrogen cyanide. The organism showed antagonism activity against the two tested phytopathogens. In the pot experiment, A. migulanus treated plants showed a statistically insignificant difference in maize plant height at P=0.05 but gave significant increases in shoot and root wet weights. The organism offered 83.33% and 71.43% protection against Enterobacter and Fusarium pathogens respectively in the pot experiment.&#x0D; Conclusion: A. migulanus solubilized phosphate in addition to other plant growth promoting properties. It showed biocontrol potentials both in vitro and in vivo and thus can be used as substitute for synthetic agrochemicals.

Manipulating Estrogenic/Anti-Estrogenic Activity of Triphenylethylenes towards Development of Novel Anti-Neoplastic SERMs.

Selective estrogen receptor modulators (SERMs) act as estrogen receptor (ERα) agonists or antagonists depending on the target issue. Tamoxifen (TAM) (a non-steroidal triphenylethylene derivative) was the first SERM approved as anti-estrogen for the treatment of metastatic breast cancer. On the hunt for novel SERMs with potential growth inhibitory activity on breast cancer cell lines yet no potential to induce endometrial carcinoma, we designed and synthesized 28 novel TAM analogs. The novel analogs bear a triphenylethylene scaffold. Modifications on rings A, B, and C aim to attenuate estrogenic/anti-estrogenic activities of the novel compounds so they can potentially inhibit breast cancer and provide positive, beneficial estrogenic effects on other tissues with no risk of developing endometrial hyperplasia. Compound 12 (E/Z-1-(2-{4-[1-(4-Chloro-phenyl)-2-(4-methoxy-phenyl)-propenyl]-phenoxy}-ethyl)-piperidine) showed an appreciable relative ERα agonistic activity in a yeast estrogen screen (YES) assay. It successfully inhibited the growth of the MCF-7 cell line with GI50 = 0.6 µM, and it was approximately three times more potent than TAM. It showed no potential estrogenicity on Ishikawa endometrial adenocarcinoma cell line via assaying alkaline phosphatase (AlkP) activity. Compound 12 was tested in vivo to assess its estrogenic properties in an uterotrophic assay in an ovariectomized rat model. Compared to TAM, it induced less increase in wet uterine wet weight and showed no uterotrophic effect. Compound 12 is a promising candidate for further development due to its inhibition activity on MCF-7 proliferation with moderate AlkP activity and no potential uterotrophic effects. The in vitro estrogenic activity encourages further investigations toward potential beneficial properties in cardiovascular, bone, and brain tissues.