Hauling ore and waste rock is a major cost factor in open-pit mines, encompassing both road maintenance and off-road truck operation. Road maintenance costs are directly influenced by traffic volume and the size of trucks using the roads. Off-road truck operating costs are impacted by various factors, including those affecting their movement, such as the direct interaction between tires and the road surface. Tire-road interaction significantly affects rolling resistance. Rougher surfaces with deeper grooves caused by track sinks lead to higher rolling resistance. This study employed laser profiling to characterize the surface roughness of five mine haul roads in two large Brazilian iron ore mines. This study evaluated the impact of these road irregularities on various operating parameters for 240-ton off-highway trucks, including rolling resistance, average travel speed, travel time, productivity, and unit transport cost. The results indicate that a 10 cm increase in road surface roughness can lead to a significant increase in rolling resistance (up to 5%), a substantial decrease in average speed (25%), a notable increase in travel time (26%), a decrease in productivity (19%), and a corresponding increase in unit transport cost (21%).