Increase In Vit Research Articles

Quercetin Mitigates Oxidative Stress, Developmental Toxicity and Teratogenic Effects Induced by High-dose Vitamin D2 in Zebrafish Embryos

The aim of the study was to determine the developmental toxicity of high-dose administration of vitamin D2 (vit. D2) and the synergistic effect of vit. D2 inco-treatment with quercetin. Zebrafish embryos at 6 hpf were treated with either vit. D2 (1, 5, 10 μg/mL) or quercetin (5, 10 μg/mL) alone. The results from visual assessment and morphological feature scoring revealed, the occurrence of different morphological abnormalities spiked and aggravated with an increase in vit. D2 dose. The percentage of hatching, heartbeats/minute, velocity, body length, and survivability rate was downregulated in high dose vit. D2 groups. Subsequently, the production of proinflammatory cytokines and intracellular ROS was upregulated in high-dose vit. D2 groups. Contrastingly, in the co-treatment of vit. D2 (5 μg/mL) and quercetin (1, 5, 10 μg/mL), the occurrence of abnormal morphological characteristics was downregulated and the percentage of survivability rate was significantly increased. The production of inflammatory cytokines, intracellular ROS, and MDA was also observed to be inhibited in co-treatment groups. The important antioxidants such as glutathione and catalase were increased in co-treatment groups. The activity of S-A-β gal and apoptosis was downregulated in co-treatment groups. Collectively, quercetin ameliorated the developmental toxicity and teratogenic effects induced by high-dose vit. D2.

Amino acids and vitamins status during continuous renal replacement therapy: An ancillary prospective observational study of a randomised control trial

BackgroundContinuous renal replacement therapy (CRRT) is associated with micronutrients loss. Current recommendations are to administer 1–1.5g/kg/day of proteins during CRRT. We aim to evaluate the net effect of CRRT on amino acids (AA), vitamins A and C (Vit A, Vit C) levels. MethodsThis is a prospective observational study embedded within a randomised controlled trial comparing two CRRT doses in patients with septic shock. CRRT was provided in continuous veno-venous haemofiltration mode at a dose of either 35ml/kg/h or 70ml/kg/h. All patients received parenteral nutrition with standard trace elements and vitamins (protein intake 1g/kg/d). We measured serum levels of glutamine, valine and alanine as well as Vit A and Vit C upon randomisation, study day four and eight. In addition, we measured a larger panel of AA in a subset of 11 patients. ResultsWe included 30 patients (17 allocated to 70ml/kg/h and 13 to 35ml/kg/h CRRT). Before CRRT initiation, mean plasma levels of glutamine and valine, Vit A and Vit C were low. CRRT was not associated with any significant change in AA levels except for a decrease in cystein. It was associated with an increase in Vit A and a decrease in Vit C levels. CRRT dose had no impact on those nutrients blood levels. ConclusionsIrrespective of dose, CRRT was associated with a decrease in cysteine and Vit C and an increase in Vit A with no significant change in other AA. Further studies should focus on lean mass wasting during CRRT.

Vitamin D partially reverses the increase in p-NF-κB/p65 immunocontent and interleukin-6 levels, but not in acetylcholinesterase activity in hippocampus of adult female ovariectomized rats

The aim of this study was to verify the effects of ovariectomy (OVX) and/or vitamin D supplementation (VIT D) on inflammatory and cholinergic parameters in hippocampus, as well as on serum estradiol and VIT D levels of rats. Ninety-day-old female Wistar rats were randomly divided into four groups: SHAM, OVX, VIT D or OVX + VIT D. Thirty days after OVX, VIT D (500 IU/kg/day) was supplemented by gavage, for 30 days. Approximately 12 h after the last VIT D administration, rats were euthanized and hippocampus and serum were obtained for further analyses. Results showed that OVX rats presented a decrease in estradiol levels when compared to control (SHAM). There was an increase in VIT D levels in the groups submitted to VIT D supplementation. OVX increased the immunocontent of nuclear p-NF-κB/p65, TNF-α and IL-6 levels. VIT D partially reversed the increase in p-NF-κB/p65 immunocontent and IL-6 levels. Regarding cholinergic system, OVX caused an increase in acetylcholinesterase activity without changing acetylcholinesterase and choline acetyltransferase immunocontents. VIT D did not reverse the increase in acetylcholinesterase activity caused by OVX. These results demonstrate that OVX alters inflammatory and cholinergic parameters and that VIT D supplementation, at the dose used, partially reversed the increase in immunocontent of p-NF-Kb/p65 and IL-6 levels, but it was not able to reverse other parameters studied. Our findings may help in the understanding of the brain changes that occurs in post menopause period and open perspectives for futures research involving VIT D therapies.

Awareness of osteoporosis among female head of household: an Iranian experience

The family structure has changed considerably in the past decade. But, women from Iran and many countries in the region and even families with similar cultural background living in other parts of the world are still responsible for childcare as well as the lifestyle adapted by the family. The present study was conducted to assess the awareness of the female head of household of osteoporosis and its complications. As part of a population-based study in two Iranian cities, Arak and Sannandaj, to study osteoporosis and its risk factors, a questionnaire on the knowledge of female head of households of osteoporosis and its complications was completed. The subsample filled out the knowledge survey along with the questionnaire on their demographic data, socioeconomic status (SES), reproductive factors, bone-related lifestyle habits, family and personal medical history, medication use, and compliance with osteoporosis medication (if used) as well as a FFQ filled out for all the 2100 participants. They also underwent a bone mineral density (BMD) test, and their serum levels of vitamin D were calculated. These data were then used to assess the factors affecting their awareness level. The knowledge of 81.3% (473) of the studied women was found to be poor on different aspects of osteoporosis and its complications. Multivariate analysis revealed that each level increase in vit D intake tertile increases the chance of higher awareness level in K-Total by 1.5 times. Each level increase in physical activity tertile was associated with a 30.6% lower chance of having a good knowledge. Considering the poor awareness level of the studied women and their poor practice of bone healthy behavior, one could conclude that more programs are needed or the content/delivery style of the existing ones should be optimized.

Association between serum vitamin D and metabolic syndrome in middle-aged and older adults and role of supplementation therapy with vitamin D.

To evaluate i) the correlation between vitamin D (vit. D) serum concentrations and metabolic syndrome (MetS); ii) the efficacy of 6 months supplementation therapy with vit. D. 200 patients were enrolled. Blood analyses and anthropometric measurements were carried out. Patients with hypovitaminosis D received an oral supplement therapy. 81% of the sample shows vit. D levels < 30 ng/mL. Rate of MetS was significantly higher in vit. D deficiency group than in vit D insufficiency (p = 0.009) and sufficiency (p = 0.002) groups. Vit. D shows a significant negative correlation with both waist circumference (WC) (ρ - 0.202 p = 0.004) and glycaemia values (FBG) (ρ -0.185 p = 0.009). After the supplementation therapy in a group of 60 subjects a significant increase in vit. D levels (p = 0.001) and a significant reduction in WC values (p = 0.001) were observed. MetS, WC and FBG appeared to be associated vit. D status and it is well-known that central obesity, with the inflammatory alterations thereto correlated that determine insulin resistance, can be considered the "primum movens" for the development of MetS.

Abstract P1-08-19: Changes in circulating vitamin D levels as a predictor for pathological response to neoadjuvant chemotherapy (NAC) in breast cancer (BC): A Dutch breast cancer trialists group (BOOG) side-study

Abstract Background: Vitamin D (vit D) status is suggested to be of prognostic value for treatment outcome in women with breast cancer. However, there are no data of the predictive value of vit D status and changes of vit D levels for response to neoadjuvant chemotherapy (NAC). Methods: A subset of patients (pts) from the NEOZOTAC trial in whom vit D data were available was evaluated. NEOZOTAC is a randomized phase III study comparing the efficacy of NCT with or without zoledronic acid (ZA) in pts with stage II/III, measurable, HER2-negative BC. Vit D deficiency and severe deficiency were defined as vit D levels of ≤ 50 and ≤25 nmol/L, respectively. Baseline vit D levels were available for correlation to pathological response of 165 pts (83 ZA-arm), while 67 pts (35 ZA arm) could be evaluated for changes in vit D levels between baseline and cycle 6. Pts who were allocated to the ZA arm should by protocol receive daily supplements of calcium/vit D 500/400 IU. Pathological response was assessed using the Miller and Payne scoring system; pathological complete response (pCR) was defined as absence of tumor cells in the tumor bed and good response was defined as ≥90% decrease of tumor cellularity. Results: Vit D was measured in 168 pts and was done in 75% of pre/perimenopausal pts and 51.3% of postmenopausal pts. There was no significant relation between baseline vit D deficiency (&amp;lt; 50 nmol/L) and pCR (pCR 25.8% for deficient pts vs. 14.1% for non-deficient pts, P = 0.06). Pts with severe vit D deficiency (&amp;lt;25 nmol/L) tended to respond less (pCR 10.5 vs 19.9%, p = 0.53). At the end of chemotherapy, good pathological responders seemed to have a slight increase in vit D levels compared to non-responders who rather showed a decrease (mean 1.11 vs. -9.71, P = 0.08). After multivariate analysis correcting for menopausal status and treatment arm, this result was significant (P = 0.03, 95% C.I. 1.004-1.055). When pts in the ZA arm were analyzed separately, again, good response was rather associated with an increase than a decrease (mean = 9.8 vs. -1.6, P = 0.12). From 17 out of 35 ZA treated pts who were vit D deficient at baseline, only 5 (29.4%) reached levels &amp;gt;50 nmol/L at the end of treatment. Conclusions: Baseline vit D status was not predictive for pCR. However, increase in vit D levels during therapy tended to be associated with better pathological response. Therefore, achieving higher vit D levels can be important. Daily suppletion with calcium/ vitamin D 500/400 might be inadequate for achieving sufficient levels after NAC. Contact information: Dr. J.R. Kroep, M.D., Ph.D., Department of Medical Oncology, email:j.r.kroep@lumc.nl or A. Charehbili, BSc. Department of Surgery and Medical Oncology, email: a.charehbili@lumc.nl or LUMC datacenter, Department of Surgery, phone +31(0)71-5263500, fax +31(0)71-5266744, email: datacenter@lumc.nl, Leiden University Medical Center (LUMC), Leiden, The Netherlands. Citation Information: Cancer Res 2013;73(24 Suppl): Abstract nr P1-08-19.

Impact of high-fat diet on antioxidant status, vascular wall thickening and cardiac function in adult female LDLR&amp;lt;sup&amp;gt;–/–&amp;lt;/sup&amp;gt; mice

Background: Western diet, rich in saturated fatty acids and cholesterol, is associated with increased cardiovascular risk. We thus investigated in female mice the influence of this diet on plasma antioxidant status, vascular wall thickening and cardiac function. Methods and Results: Adult female C57BL/6J wild type (WT) and LDLR–/– mice were fed a normal diet (ND) or a high-fat diet (HFD) for 17 weeks. HFD induced an increase in plasma lipids and vitamin C (Vit C) levels in both groups but at a much higher level in LDLR–/– and a decrease in plasma ascorbyl free radical levels to Vit C ratio (an endogenous oxidative stress index) in LDLR–/–. We only found a slight decrease in circulating antioxidant status evaluated by the Oxygen Radical Absorbance Capacity (ORAC) assay in WT, but not in LDLR–/–. Echocardiography evidenced an increase in arterial wall thickness in aortic arch at atherosclerosis predilection sites in HFD LDLR–/– as compared to ND LDLR–/– and HFD WT. This result was confirmed by histology. Further-more, histological examination of aortic valves showed an increase in atherosclerotic lesions. Our study, using echocardiography, show that chronic HFD does not induce any major modifications of systolic function in the both mice groups. Conclusions: High-fat intake in mice causes serious disturbances in lipid plasma levels associated to variations of circulating antioxidant status due, at least in part, to an increase in Vit C. At this stage, atherosclerotic lesions, observed in aortic arch and valve, do not impair cardiac function in HFD-fed mice.

Enhancement of gilthead seabream (Sparus aurata) larval growth by dietary vitamin E in relation to two different levels of essential fatty acids

The objective of this study was to determine the effect of dietary vitamin E on gilthead seabream (Sparus aurata) growth and survival, at two different highly unsaturated fatty acids (HUFAs) levels. Eighteen days old gilthead seabream larvae were fed four formulated experimental diets combining two different dietary levels of HUFAs (M: medium 2.5 + 1.5, DHA + EPA, H: high 5 + 2.5 DHA + EPA g per 100 g) with two different levels of vitamin E (M: medium 540 mg kg−1, H: high 2900 mg kg−1): MM, MH, HM, HH (HUFA/vitamin E). After 2-week feeding trial, the average survival rate was 52.6% and there were no significant differences found among treatments. Increase in vitamin E up to high level markedly improved larval growth, particularly when dietary HUFA levels were lower, suggesting a higher protection value when these fatty acids are more limiting. At medium dietary HUFA levels, increase in vitamin E from medium to high level enhanced larval growth performance in terms of total length. Moreover, increase in vit E enhanced HUFAs content in the larval polar lipids denoting the anti-oxidative effect of vitamin E.