Increased Milk Production Research Articles

Association of CXCR1 Gene Polymorphism With Clinical Mastitis and Performance Traits in Murrah Buffalo.

This study revealed the presence of single nucleotide polymorphisms in the CXCR1 gene and their association with performance traits and mastitis incidence in Murrah buffalo. The targeted SNP rs211042414 (T > C) at the g.106216468 locus in partial exon 2 of the CXCR1 gene was genotyped using PCR amplification and restriction enzyme digestion by Alu I, Bsa I, Dde I, Ava I, Hind III, EcoRV, Hae III, and Hae II restriction enzymes. The genotypic frequencies revealed three genotypes: CC, CT, and TT, with the C allele being the most prevalent (0.79) compared to the T allele (0.21). Targeted SNP showed significant association with the incidence of clinical mastitis incidence, and the study revealed that the TT genotyped animals showed a higher incidence of clinical mastitis compared to the TT and CT genotyped animals in the targeted population. Furthermore, least squares analysis revealed that targeted performance traits, viz. 305 days milk yield, total milk yield, and lactation length, were significantly associated with the genetic variants of the CXCR1 gene. TT genotyped animals exhibited higher milk yield than CT and CC genotyped animals, indicating a positive association between the T allele and increased milk production. These findings revealed that there is a scope for the genetic improvement of Murrah buffalo. Incorporating the identified CXCR1 gene polymorphisms into existing selection criteria can help to improve disease resistance and milk production traits in Murrah buffalo. However, further validation with a larger sample size is necessary to strengthen the observed associations and ensure their practical applicability.

Constraint Analysis and Suggestion of Respondents Regarding Dairy Management Practices in Semi-Urban and Rural Areas

India needs to increase milk production which can be possible by narrowing down the gap between the existing technology and their adoption. Ensuring domestic demand and enduring top most position in the world, India needs to produce 300-400 million tones of milk by 2050 (Vision 2050, 2015). The dairy is a business which provides the continuous source of income to the occupants and in case of landless person who are economically week, livestock farming is a better option. The research was based on a survey done in 2022-23 to analyzing constraint and suggestion of respondents regarding dairy management practices in semi-urban and rural areas of Fatehpur district in Uttar Pradesh. 15 respondents from each selected semi-urban and rural villages were randomly selected to constitute 120 samples (60 semi-urban + 60 rural). The perceived constraints have been studies under different aspects like: personal constraints, economical constraints, technological constraints and marketing constraints. The semi-urban respondents has perceived most serious type constraints viz., lack of poor economic condition of farmers, dysfunctional of animal insurance schemes, A.I. and BAIF centers are away from the village, lack of remunerative milk prices. Whereas rural respondents has perceived most serious type constraints viz., lack of interest of other family members, veterinary medicines are very costly for treatment of sick animals, A.I. and BAIF centers are away from the village, poor transportation also affects adversity in proper disposal of milk products. Suggestions as perceived by semi-urban respondents viz., ‘educational programmes regarding cattle breeds improvement for higher milk production should be initiated’ was ranked first whether in case of rural dairy farmers ‘government must provide marketing facilities at village level for selling the milk and milk products’ was ranked first.

Effects of Smart Farming on the Productivity of Korean Dairy Farms: A Case Study of Robotic Milking Systems

The Korean agricultural sector faces increasing challenges such as an aging population, labor shortages, and the liberalization of agricultural markets. To overcome these challenges, the Korean government has striven to enhance the competitiveness of agriculture by introducing AI-based technologies to the agricultural sector, labeling this as smart farming. This study estimates farm-level benefits of adopting smart farming technologies, robotic milking systems, in Korean dairy farms. The benefits are estimated by comparing the productivity (i.e., the savings of labor input, increased calf production, and increased milk production) of adopting and non-adopting farms. Our study uses the propensity score matching method to address potential problems from confounding factors, sample selection bias, and the small number of adopters. Our results show that farms that adopted robotic milking systems produced 0.10 to 0.11 more calves per year than farms that did not adopt the system. The adopters also increased milk production by 2.44 kg to 2.88 kg per head/day, while reducing labor input by 0.15 to 0.30 per head/week. However, the reduced labor input was not statistically significant. When the analysis was extended to regard the farm characteristics, the labor input became significant from small and family-run farms. We also found that the increase in the number of calves produced per head was statically significant from small farms, family-run farms, and farms with successors. The increased milk production per head was statistically significant from large farms, farms employing hired workers, and farms with successors. Our findings suggest that the Korean government continue promoting smart farming technologies such as the robotic milking system to increase the adoption rate. The findings can also provide useful information about target markets of this technology, which can be used to increase the adoption rate and ultimately enhance the sustainability and competitiveness of the Korean dairy industry.

Estimation of Marketed Surplus and Marketing Efficiency of Milk in Andhra Pradesh, India

A study was conducted to analyze milk's marketed surplus and marketing efficiency in the Vishakhapatnam and Chittoor districts of Andhra Pradesh. The study randomly selected 80 dairy farmers from four villages in Chittoor and Vishakhapatnam districts. According to the study, average milk production and marketable surplus increase as herd size increases. The average marketed surplus is highest for the large size category (58.75 lit/day) followed by Medium (45.40 lit/day) and small size category (19.01 lit/day). Because of the lowest price spread and highest producer share in consumers' rupee, the most efficient marketing channel was producer-consumer (Channel-I), followed by producer-creameries-consumer (Channel-II). The study suggests that producers' choice of supply network influences their profitability.

The microbiota: a key regulator of health, productivity, and reproductive success in mammals.

The microbiota, intensely intertwined with mammalian physiology, significantly impacts health, productivity, and reproductive functions. The normal microbiota interacts with the host through the following key mechanisms: acting as a protective barrier against pathogens, maintain mucosal barrier integrity, assisting in nutrient metabolism, and modulating of the immune response. Therefore, supporting growth and development of host, and providing protection against pathogens and toxic substances. The microbiota significantly influences brain development and behavior, as demonstrated by comprehensive findings from controlled laboratory experiments and human clinical studies. The prospects suggested that gut microbiome influence neurodevelopmental processes, modulate stress responses, and affect cognitive function through the gut-brain axis. Microbiota in the gastrointestinal tract of farm animals break down and ferment the ingested feed into nutrients, utilize to produce meat and milk. Among the beneficial by-products of gut microbiota, short-chain fatty acids (SCFAs) are particularly noteworthy for their substantial role in disease prevention and the promotion of various productive aspects in mammals. The microbiota plays a pivotal role in the reproductive hormonal systems of mammals, boosting reproductive performance in both sexes and fostering the maternal-infant connection, thereby becoming a crucial factor in sustaining mammalian existence. The microbiota is a critical factor influencing reproductive success and production traits in mammals. A well-balanced microbiome improves nutrient absorption and metabolic efficiency, leading to better growth rates, increased milk production, and enhanced overall health. Additionally, it regulates key reproductive hormones like estrogen and progesterone, which are essential for successful conception and pregnancy. Understanding the role of gut microbiota offers valuable insights for optimizing breeding and improving production outcomes, contributing to advancements in agriculture and veterinary medicine. This study emphasizes the critical ecological roles of mammalian microbiota, highlighting their essential contributions to health, productivity, and reproductive success. By integrating human and veterinary perspectives, it demonstrates how microbial communities enhance immune function, metabolic processes, and hormonal regulation across species, offering insights that benefit both clinical and agricultural advancements.

Effects of dry matter concentration in grass silage on milk production of dairy cows fed concentrates high or low in metabolizable protein concentration

Studies have shown that extended wilting of grass before ensiling can improve the metabolizable protein (MP) value of the grass silage, both from increased rumen microbial crude protein (MCP) yield and ruminally undegraded crude protein (RUP). We hypothesised that extending the wilting of grass before ensiling can increase milk and milk protein production in dairy cows. Consequently, increased silage MP can reduce the need for MP in concentrate, estimated as amino acids absorbed in the small intestine (AAT20). To test this, a continuous feeding experiment was conducted, with 48 early- to mid-lactation Norwegian Red dairy cows. Treatments were 2 wilting levels of grass silages: 260 vs. 417 g dry matter (DM) per kg, and 2 qualities of concentrates differing in MP, in a 2 × 2 factorial arrangement (n = 12). The concentrates were low MP (LMP); 104 g AAT20/kg DM, and high MP (HMP); 123 g AAT20/kg DM, supplied at 8.75 kg DM/cow per day. The cows had ad libitum access to the grass silages. The experiment lasted for 11 weeks. The first 2 weeks, where cows received identical diets, were used as the covariate period. Those data together with data collected the last 4 weeks were used for statistical evaluation of treatments. Increasing DM concentration in silage reduced lactic acid, ammonia nitrogen and proportion of methionine in the grass silage, while it increased residual water-soluble carbohydrates, pH, proportion of aspartic acid and rumen degradability of NDF. There was no difference between treatments in daily silage DM intake (13.1 kg), milk yield (30.2 kg) or milk composition. However, feeding HMP increased urea and uric acid in urine, and urea in blood plasma, thereby reduced N efficiency. No major differences were found for rumen pH, or purine derivatives to creatinine index in urine, as indication for MCP. There was significantly more histidine in blood plasma when cows were fed HMP concentrate. In conclusion, increased DM concentration in silage and HMP concentrate did not increase milk production or milk protein yield in this study.

Lactation Massage and Hypnobreastfeeding Relaxation Techniques in Breastfeeding Mothers

Breast milk is a kind of food that is good for meeting all the physical needs of babies. Breast milk contains most of the nutritional elements, hormones and immunity of a baby. After that the baby will only get breast milk until the baby is six months old, after the baby is six months old the baby will be given complementary food for breast milk until the baby is even two years old. Purpose: this research is to find out the description of nursing care for normal postpartum mothers who experience ineffective breastfeeding at Koja Jakarta Hospital in 2023 with lactation massage and hypnobreastfeeding relaxation techniques. Methodology: the research used is a descriptive researcher with a case study design that will be carried out in June 2023. Results: a study of patients who experienced ineffective breastfeeding by means of lactation massage and hypnobreastfeeding relaxation techniques, could increase milk production, reduce breast swelling and reduce pain. This can happen because of the willingness of the client to follow. Conclusion: lactation massage techniques and hypnobreastfeeding relaxation techniques can be done when complaints of a little milk coming out, anxiety, swelling in the breasts of post partum mothers are normal.

Development of Biscuits from Winged Beans (Psophocarpus tetragonolobus L.) as a Primary Source for Milk Production

Background: The high prevalence of stunting, notably in Aceh at 31.2%, highlights the urgent requirement for effective nutrition interventions. Utilizing local resources like winged bean flour in food products can play a vital role in enhancing maternal and infant health, addressing malnutrition, and improving breastfeeding outcomes Objective: This study aims to develop biscuits from winged beans (Psophocarpus tetragonolobus L.) as a primary source for enhancing milk production. Winged beans are known to be rich in protein and essential nutrients for the health of breastfeeding mothers. Methodology: This study employs a true experimental design involving clinical trials on rabbits, proximate analysis, and sensory acceptance testing. In the first year, the focus is on developing winged bean biscuit formulas, conducting nutritional and contamination tests, and measuring weight and milk volume in animal trials. The research will take place from March to August 2024 at various locations, including the Nutrition Department at Aceh Health Polytechnic and Syiah Kuala University. In this research, biscuits were produced with varying additions of winged bean flour at 0%, 10%, 15%, and 20% in the biscuit recipe. Results: The results indicate that biscuits containing winged bean flour can increase milk production in female rabbits and show significant weight gain after biscuit consumption. Proximate analysis reveals good nutritional content, while testing for harmful metal contamination ensures product safety. Organoleptic acceptance testing shows that these biscuits are well-received by consumers. These findings affirm the potential of winged beans as a local food source that can support the health of mothers and children, contributing to efforts to combat malnutrition in Indonesia. Conclusion:. Biscuit made from winged bean flour can enhance milk production and weight gain in female rabbits, while offering good nutritional content and being free from contamination. These findings support the potential of winged beans as a local food source to address malnutrition in Indonesia.

Follicular persistence, oestradiol level and conception on the first and second day of oestrus and influence of thermal stress in cross-bred dairy cows manifesting prolonged oestrus

Increasing incidence of prolonged oestrus reported in cross-bred dairy cows bred through artificial insemination (AI), is attributed to various causative factors and underlying mechanisms. The present study was to assess the pattern of follicular growth, oestrogen secretion, and conception on the first and second day of oestrus and to understand the influence of thermal stress as a causative factor for oestrus prolongation. The year-round study involved 52 crossbred cows intensively managed under a hot humid climate, during their second and third months postpartum. Animals were examined for the status of ovarian follicles, oestrogen level, and conception to AI on Day 0 and Day 1 of oestrus confirmation and the findings were compared across seasons. While oestrus detection rate (ODR) and conception of AI were lowest during the summer season attributable to maximum TS, better ODR (P<0.05) and more prolonged oestrus (P<0.01) occurred during the seasons of favourable climate having least TS and better green fodder availability. Besides, the occurrence of larger follicles and almost similar oestradiol levels on Day 0 and Day 1 of oestrus, better conception rate for double AI (63.16 %) than total AI (48.48 %) indicated prolonged oestrus and involvement of delayed ovulation among the causation. Comparison of the seasonal pattern of follicular growth, oestrogen level, and conception to AI between Day 0 and Day 1 further clarifies the influence of TS and increased milk production as the potential contributors to the occurrence of prolonged oestrus in cross-bred cows under the hot humid tropical climate. Keywords: Crossbred cow, Oestrogen, Prolonged oestrus, Thermal stress

Effects of pre- and postpartum dietary fat sources (soybean oil versus linseed oil) on lactation performance and blood metabolites in transition dairy cows

This study investigated the effects of supplementation with two calcium salt fatty acid sources (soybean oil (CaSO) and linseed oil (CaLN)) before and after parturition on lactation performance and blood metabolite profiles in dairy cows. A study was conducted with twenty-four multiparous Holstein cows (parity = 3.12 ± 0.9 and Backfat thickness = 21.77 ± 1.98) that were divided into a 2 × 2 factorial design 21 days before expected calving. The aim was to investigate the interplay between prepartum (CaSO or CaLN, at 2 % of dry matter) and postpartum (at 1.4 % of dry matter) fat supplementation on lactation performance and blood metabolite profiles. Initially, cows were grouped according to the source of prepartum fat (either CaSO or CaLN, with 12 cows in each group). Postpartum, these groups were further subdivided according to whether the fat source was administered continuously (LN-LN, SO-SO) or alternately (LN-SO, SO-LN) over a 28-day period. This resulted in four different treatment groups, each with six cows. The fat supplements contained 84 % fat and 9 % Ca. No statistically significant differences were found in dry matter intake (DMI), colostrum yield or most concentrations of blood metabolites (insulin, cholesterol, glucose, triglycerides, total protein, albumin, β-hydroxybutyric acid (BHB), non-esterified fatty acids (NEFA), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and urea-N). However, cows fed CaSO prepartum had a significantly higher plasma concentration of insulin-like growth factor-1 (IGF-1) than cows fed CaLN. Cows fed CaSO had higher backfat thickness (BFT) from day −21–0 than those fed CaLN (P = 0.05). Notably, cows in the SO-SO group lost the most weight, while cows in the SO-LN group lost the least, especially on days 21 and 28 postpartum (P = 0.05). Switching from soybean oil prepartum to linseed oil postpartum (SO-LN) significantly increased milk production in the first 28 days of lactation compared to other diets. In addition, the fat content in milk was influenced by the type of fat supplementation prepartum, with cows receiving CaSO having a lower milk fat percentage than those receiving CaLN (P < 0.05). The results on blood parameters suggest potential benefits of the SO-LN diet for postpartum cows, as emphasized by stable BHB concentrations and the highest IGF-1 concentrations, especially without the elevated BHB concentrations typically associated with the SO-SO diet. The blood concentrations of total protein, urea-N, triglycerides and NEFA were not affected by the treatments. Also, the dietary change did not adversely affect liver function, as shown by unchanged AST and ALT concentrations. Therefore, the results suggest that a dietary intervention involving the administration of soybean oil prepartum and switching to linseed oil postpartum (SO-LN strategy) has the potential to improve milk yield during the early lactation period.

Pemberdayaan Masyarakat Melalui Pelatihan Membuat Makanan Olahan Dari jantung Pisang Untuk Kelancaran Produksi ASI di Link. Temu Putih Kota Cilegon Tahun 2024

Breast milk is the primary and most essential food for newborn babies aged 0-6 months. Several factors can affect the amount of breast milk production, including nutritional patterns, support, breast care, baby's sucking, and socio-cultural factors. It is important to understand the nutritional factors for breastfeeding mothers because their nutritional needs must be increased to enhance breast milk production. One effort to boost breast milk production is through the consumption of foods that influence lactation, such as banana blossom preparations. The dietary fiber in banana blossoms is beneficial for increasing milk production because it contains lactagogum, which has the potential to stimulate oxytocin and prolactin hormones. These components, such as alkaloids, polyphenols, steroids, flavonoids, and other substances, are effective in enhancing and facilitating breast milk production. The purpose of this community service, in the form of training to make food preparations from banana blossoms, is to empower the community, particularly pregnant and postpartum women, by enhancing their skills in utilizing banana blossoms as a food that can be processed to increase breast milk production. This community service is carried out by providing training on how to make banana blossom-based food, specifically turning it into nuggets, for pregnant and postpartum women in the Temu Putih area, Cilegon City.

Solar irrigation system: Analysis of the solution for livestock production in North Cameroon

This article examines the impact of implementing a solar irrigation system on livestock production in North Cameroon, a region marked by climatic challenges and increasingly dry conditions with 600 to 1,200 mm of rainfall per year and temperatures between 30°C and 40°C. Livestock farmers in the region rely heavily on natural pastures and forage crops to feed their livestock, but these resources are insufficient during periods of drought. The area is home to around 2 million head of cattle, with an estimated 500,000 to 700,000 head of sheep and 300,000 to 500,000 head of goats. Our methodology involved a comparative before-and-after analysis, where data on crop yields, pasture quality and animal performance were collected. Statistical simulation was used to assess improvements in pasture biomass, forage yield and animal performance. The results show significant improvements if the solar irrigation system is installed, with: a 30% increase in biomass; a 50% increase in forage yield with a 16.7% gain in animal weight; a 20% increase in milk production; a 30% reduction in forage procurement costs; and a 33.3% increase in income generated. These results indicate that solar irrigation will have a positive impact on farm productivity and profitability. The installation of a solar irrigation system in Northern Cameroon represents a sustainable and economically viable solution for improving livestock production in a region vulnerable to drought.In sub-Saharan Africa, solar irrigation systems have been successfully implemented in several countries, including Kenya, Ethiopia, Mali and Burkina Faso. These projects have demonstrated the viability of this technology in semi-arid and arid environments, with notable improvements in agricultural production and water management. The climatic and geographical conditions in Northern Cameroon are similar to those in the regions where solar irrigation systems have been tested.

MORE MILK, FEWER FARMS, and REGIONAL CONCENTRATION: MAPPING TRANSFORMATIONS IN CALIFORNIA’S DAIRY INDUSTRY

ABSTRACT The transformation of California’s dairy industry is a prime example of structural change in agriculture. The state has been on the leading edge of changes in the geography of food and agriculture for 150 years, and many characteristics of modern large-scale agriculture emerged—or reached their culmination—in California. We examine the spatial transformation of dairy production in California as a window into the potential futures for the dairy industry both within California and elsewhere. We use data from county agricultural reports and USDA Censuses of Agriculture data to demonstrate, through a series of original maps and animations, the dramatic increase in production of milk, the rapid fall in the number of dairies, and the spatial redistribution of the industry over the past 40 years. These visualizations reveal illustrative spatial patterns and insights into rapid changes in the geography of dairy production—and agriculture more generally—within California. Based on these visualizations and analyses, we propose a framework for understanding the intersecting phenomena of dairy transformations in California: regional concentration, industry consolidation, and farm-level intensification. In explaining how these processes are nested, overlapping, and multiscalar, we offer an account of past and current trajectories, while examining the applicability and implications of these findings for other dairy-producing regions into the future.

Efficiency of use of land and feed resources to increase milk productivity of cows

Efficiency of use of land and feed resources to increase milk productivity of cows

Artificial Insemination: A New Horizon for Scientific Goat Breeding in Kashmir’s Backyard Farming Sector

Background: Artificial insemination a well-established technique in cattle and buffalo farming, now increasingly adopted in goat farming involves collecting semen from male animals and transferring it to female animals, to disseminate valuable genetic material to bring genetic upgradation, increases milk production, and enhances breeding efficiency of flocks. Methods: Artificial insemination (AI) was performed on 266 goats using fresh and frozen semen from 2021 to 2024 on experimental basis. The data obtained were analyzed on percentage basis. Result: The overall kidding percentage of 30.08% was observed in the present study. Notably, fresh semen yielded a higher kidding percentage (32.69%) compared to frozen semen (29.44%). This study demonstrates the potential of AI in enhancing the genetic quality of backyard goat farming in Kashmir, where quality bucks are often sold at a young age for meat production. By leveraging AI, farmers can improve the genetic makeup of their goat flocks, leading to better productivity and sustainability.

Individualised feeding of concentrate supplement according to milk yield did not increase milk production in pasture-grazed cows near peak lactation

ABSTRACT In grazing systems, feeds other than pasture are often used to increase dry matter intake and milk production. The study aimed to evaluate the effect of individually feeding pasture-grazed dairy cows near peak lactation based on their individualised milk production compared with a flat-rate feeding regime. All cows in the flat-rate treatment were offered 4 kg wet weight (3.6 kg DM) of concentrate/d. In the individualised feeding treatment, cows were offered 6, 4, and 2 kg wet weight/d (5.4, 3.6 and 1.8 kg DM/d) in the high, moderate, and low production groups, respectively. Individualised feeding of concentrates based on cow milk production did not alter milk or milk component yield at a herd level, but increased milk yield and tended to increase yield of fat, protein, and lactose in cows in the medium milk yield tertile. Individualised feeding reduced milk protein yield in cows in the low milk yield tertile, compared with flat rate feeding. The results do not support individualised feeding in grazing dairy systems where the maximum level of concentrate offered/ is ≤ 6 kg DM/d and milk production/cow ≤30 kg milk/d or ≤2.5 kg fat and protein/d.

PSV-2 Rumen fluid metabolomics of dairy cows fed combinations of essential oil blend, onion peel extract and prebiotics using the RUSITEC system

Abstract Dairy products have a crucial role in the diets of over 6 billion people worldwide, providing essential nutrients such as energy, high-quality protein, and micronutrients. With dairy cows accounting for nearly 81% of global milk production, strategies to increase milk production sustainably are vital, especially with the U.S. being the second-largest producer. Essential oil blends (EOB), onion peel extract (OPE), and prebiotics such as mannan oligosaccharides (MOS) and galacto-oligosaccharides (GOS) offer promising avenues to enhance bovine milk production. These additives have the potential to modify rumen ecology and fermentation activity, while also mitigating greenhouse gas emissions in dairy cows. Therefore, this study investigated the effects of combining EOB, OPE, GOS, and/or MOS on specific rumen metabolites and metabolic pathways using the rumen simulation technique (RUSITEC) system. Rumen-cannulated non-lactating Holstein Friesian cows (n = 3) were used as rumen fluid donors. Total mixed ration (TMR) consisted of corn silage (60%), alfalfa hay (20%), and concentrate (20%). Using a completely randomized design, 16 fermentation vessels were divided into 4 groups with 4 replicates each over a 9-d period. The treatments included a control group (CON; TMR only), GEO (TMR+GOS+EOB+OPE), MEO (TMR+MOS+EOB+OPE), and OLEO (TMR+OLG+EOB+OPE). The EOB comprised of garlic, lemongrass, cumin, lavender, and nutmeg (4:2:2:1:1), while OLG contained GOS and MOS in equal proportions. EOB was added at 10 µL/g, and OPE, GOS, MOS, and OLG were included at 30 mg/g TMR. Rumen effluents were collected over 5 d for quantitative untargeted metabolome analysis. Analysis of rumen effluents using liquid chromatography-mass spectrometry and MetaboAnalyst 5.0 revealed 495 detected and identified metabolites. Volcano plot analysis showed no significant differences (P ≥ 0.05) in metabolite abundance between the CON and MEO, GEO, or OLEO treatments. Partial least squares-discriminant analysis (PLS-DA) also indicated no clear separation between the CON and treatment groups, suggesting no significant alteration in the metabolome. Compared with CON, the relative concentrations of dopamine 4-O-glucuronide, proline, and histamine were less while pyrocatechol was greater in MEO group. Isomer 1 of 2-hydroxyhepta-2,4-dienedioic acid, histamine, and dopamine 4-O-glucuronide were less while 2-aminomuconic acid and isomer 1 of hulupinic acid were greater in the GEO group. Histamine was less and hydroxypyridine was greater in the OLEO group. Phosphonate and phosphinate metabolism tended to be upregulated in OLEO. Meanwhile, metabolic pathways related to amino acids, vitamins, nitrogen metabolism, and primary bile acid biosynthesis remained unaltered by MEO, GEO and OLEO treatments. In summary, the study demonstrated that the inclusion of MEO, GEO, and OLEO had no adverse effects on rumen metabolites. Future research should focus on combinations of nutraceuticals that could positively impact nutrient metabolism and production performance of dairy cows.

PSXI-30 Economic impact of pre-training on dairy cattle automatic milking system acclimation

Abstract Pre-training in automatic milking systems (AMS) has the potential to increase milk production and milk quality. In a recent study, cows that experienced pre-training (TRT; i.e. intentional training access with an AMS prior to first AMS milking) had greater milk yield and fat percentages compared with no pre-training (38.4 vs. 37.6 ± 0.2 kg/d; 4.75 vs. 4.38 ± 0.06%; P &amp;lt; 0.01, and &amp;lt; 0.01 respectively). Similarly, cows without pre-training had greater somatic cell count (SCC; 298,860 vs. 188,220 ± 10,700 cells/mL; P &amp;lt; 0.001). The time required for pre-training could be a limiting factor (40 ± 10 h/wk), and the economic implications of labor versus long-term benefits must be examined. To that end, a stochastic simulation tool was developed to consider the increased costs of labor and the potential benefits of improved milk yield, milk fat percentage, and decreased somatic cell count. The GRKS distribution with minimum, mean, and maximum values was used to model increases in milk yield (0, 1.4, 1.8 kg), fat percentage (0, 3.5%, 4.5%), and labor cost ($0.0011, $0.0016, $0.0020 per h) and decreases in SCC (0, 120, 130 x 103 cells/mL) per hundredweight of milk produced. Baseline values for milk production ranged from 14.7 to 59.2 kg/cow/d in 4.4 kg increments with fat percentage of 3.5% and SCC of 312,000 cells/mL. The model assumed a minimum increase across milk yield, fat, and SCC of 0 (no change). The Simetar add-on in Microsoft Excel simulated 500 iterations across three herd size scenarios (60, 120, and 480 cows) and calculated the marginal return on investment across a 90-d period for all milk production ranges. Regardless of starting milk production level, pre-training had a positive economic impact across all levels (30 to 50 h) and herd sizes (Table 1). Financial impact per cow per d with input of 30, 40, and 50 h ranged from $0.58 to $0.73, $0.56 to $0.71, and $0.54 to $0.70. The majority of simulations returned a positive marginal impact for pre-training. If pre-training resulted in a loss, it ranged from $242 to $1,937, $323 to $2,585, $405 to $3,246, across 30, 40, and 50 hours, respectively. Based on these results, the investment in pre-training time was consistently beneficial for simulated herds.

Penerapan Pijat Oksitosin Terhadap Produksi ASI di Ruang Cempaka RSUD Dr.Soehadi Pridjonegoro Sragen

Background: Breast Milk (ASI) is a fatty emulsion in lactose protein and inorganic salts secreted by the mother's mammary gland, useful as food for infants. Breast milk is difficult to achieve because one of them is breast milk that does not come out or does not run smoothly. One of the non-pharmacological arrangements to help smooth the release of breast milk can be done by oxytocin massage. Objective: Knowing the results of the implementation of oxytocin massage to breast milk production at the Cempaka Ward of dr. Soehadi Pridjonegoro Sragen Hospital. Method: This study is a descriptive study in the form of a case study conducted on 2 postpartum mother respondents by massage oxytocin for 2 consecutive days with a frequency of 2x a day for 10-15 minutes. Result: The study of oxytocin massage in both postpartum mothers on the first day has not changed breast milk production. Then on the second day of breast milk production, the two respondents showed an increase, evidenced by the increase in breast milk, empty breasts after catching up, and the breasts looked full before breastfeeding. Conclusion: There is an increase in breast milk production before and after giving oxytocin massage to postpartum mothers in the Cempaka Room of dr. Soehadi Prjonegoro Sragen Hospital.

Selenomethionine Promotes Milk Protein and Fat Synthesis and Proliferation of Mammary Epithelial Cells through the GPR37-mTOR-S6K1 Signaling.

Selenomethionine (SeMet) is an important nutrient, but its role in milk synthesis and the GPCR related to SeMet sensing is still largely unknown. Here, we determined the dose-dependent role of SeMet on milk protein and fat synthesis and proliferation of mammary epithelial cells (MECs), and we also uncovered the GPCR-mediating SeMet function. At 24 h postdelivery, lactating mother mice were fed a maintenance diet supplemented with 0, 5, 10, 20, 40, and 80 mg/kg SeMet, and the feeding process lasted for 18 days. The 10 mg/kg group had the best increase in milk production, weight gain of offspring mice, and mammary gland weight and acinar size, whereas a higher concentration of SeMet gradually decreased the weight gain of the offspring mice and showed toxic effects. Transcriptome sequencing was performed to find the differentially expressed genes (DEGs) between the mammary gland tissues of mother mice in the 10 mg/kg SeMet treatment group and the control group. A total of 258 DEGs were screened out, including 82 highly expressed genes including GPR37 and 176 lowly expressed genes. SeMet increased milk protein and fat synthesis in HC11 cells and cell proliferation, mTOR and S6K1 phosphorylation, and expression of GPR37 in a dose-dependent manner. GPR37 knockdown decreased milk protein and fat synthesis in HC11 cells and cell proliferation and blocked SeMet stimulation on mTOR and S6K1 phosphorylation. Taken together, our data demonstrate that SeMet can promote milk protein and fat synthesis and proliferation of MECs and functions through the GPR37-mTOR-S6K1 signaling pathway.