Introduction: This study investigates the impact of iron supplementation on physiological and physical variables among active female students. Iron is essential for oxygen transport and is routinely depleted among iron runners due to exercise intensity. Considering the complicated relationship between exercise intensity, participants’ current health status, eating habits, and normal iron levels, this study seeks to illuminate whether iron supplementation benefits female athletes recruited among students from Burdwan University’s M.P.Ed. Department. Materials and Methods: This study targets a population of 10 female students aged between 22 and 27 years. Participants were selected on the basis of the homogeneity of their age, height, and weight. The study design was conducted as pretest/posttest research. Participants were provided with iron supplements, Punarnaba Moondur, with 250 mg of iron to be taken twice a day with honey and fed as specified. Hemoglobin level and various physiological and physical test parameters were chosen as primary outcomes. Using a t-test setting, P = 0.05, the difference between pre- and postparameters was analyzed. Results: Analysis indicates significant positive variations in postintervention hemoglobin level results in relation to preintervention results. Thus, the iron level was successfully increased. Comparably, various physiological functions and physical fitness parameters were improved among the participants: Their muscles worked more efficiently, and physical capabilities were generally enhanced. Conclusion: The use of Punarnaba Moondur as an iron supplement significantly increased the amount of hemoglobin among the active students. This development led to improved physiological and physical capacity. Parametric supplementation can be used to correct exercise-induced iron deficiency in female athletes to increase exercise performance and health outcomes. This study highlights the value of attenuation nutrition as an intervention tool against exercise-induced iron depletion.