Introduction. Breast milk from birth is extremely important for the care, health and development of premature and sick babies. Neonatal departments of obstetric and pediatric hospitals play a key role in maintaining breast feeding. Aim of the study . Breastfeeding practice evaluation support in the pathology of newborns and premature babies department of Tambov Regional Children’s Clinical Hospital 4 years after receiving the status of WHO/UNICEF “Baby-Friendly Hospital”. Patients and methods . 356 mothers were surveyed at admission and 327 at discharge from the department. To process the results we used the IBM SPSS.19 statistical program. We determine distribution frequencies, average values, standard deviation, Student’s t -coefficient. Results . The introduction of 10 successful breast feeding steps in neonatal units for sick and premature infants has resulted in an increase in breast feeding rates. Breast feeding rates were 89.4 % at discharge; 71.9 % at admission ( p < 0.0001), including exclusive breast feeding - 66.5% and 45.3 %, respectively ( p < 0.0001). Conclusion . In neonatal unit of Tambov Regional Children’s Clinical Hospital successfully supported breast feeding, based on current international recommendations. Nevertheless, breast feeding rates can be significantly improved by achieving continuity in the work of maternity hospitals and children’s hospitals, as well as optimizing the work of expression, storage and use of expressed native breast milk in the department.