Background: Low Back Pain (LBP) is one of the most common diseases affecting many persons including Healthcare Professionals (HCPs). LBP affects the quality of work and daily activities in HCPs. However, many factors contribute to LBP including age, Body Mass Index (BMI) and working experience. Purpose: The objective of this study was to assess the prevalence of LBP and examine the potential risk factors associated with LBP in Chinese HCPs (surgeons and surgical nurses). Method: A cross-sectional survey performed from October 2023 to January 2024. Quebec Back Pain Disability Scale (QBPDS) was used to assess the rating of LBP impairment. As well as Ovako Working Posture Assessment System (OWAS) is used to assess the working postures of HCPs. In addition to five more questions with yes/no answers. Results: A total of 250 surgeons and surgical nurses were collected from six hospitals with a mean age of 45.8 ± 11.0 years old, 47 females and 203 males. The BMI was 25.07 ± 1.49 kg, and working experience was 20.30 ± 10.0 years. Working hours in a week range from < 50 to > 55 hours. However, the OWAS action category was 1 in 109, 2 in 107, 3 in 30, and 4 in 4 participants. The majority of participants with mild LBP (90.4%), half of them had neck pain, and 26% with knee pain. The QBPDS scores of HCPs with LBP were 10.88 ± 4.78. Moreover, the risk factors associated with LBP include long working hours and incorrect postures of the trunk and lower extremities. As well as notice the orthopedic surgeon was the highest risk among HCPs (p = 0.046, 0.005). Conclusion: LBP is a common disorder in surgeons and surgical nurses and is associated with different factors including age, work experience, BMI, and abnormal working posture.