Inclusion Of Curcumin Research Articles

Development and characterization of polyvinyl alcohol/chitosan crosslinked malic acid composite films with curcumin encapsulated in β-cyclodextrin for food packaging application

Considering that fruits are vulnerable to damage and waste during stockpiling, transport and marketing. Given this, an innovative curcumin inclusion compound (Cur@β-CD) was devised in this study to introduce oil-soluble curcumin (Cur) into water-soluble polyvinyl alcohol (PVA) materials, thereby fabricating food packaging films endowed with excellent properties. DPPH test manifested that the oxidation resistance for PCOMC-Cur@β-CD film was 95 % above PVA material. It was ascribed to the fact that the Cur@β-CD elevated the water solubility of Cur while the increase of water solubility heightened the antioxidant effect for Cur in the film. Additionally, the chitosan (CS) was crosslinked with malic acid (MA), which elevated the barrier property of the film, reduced the amount of oxygen transmission and further retarded the oxidation reaction of the fruits for packaging. The antibacterial test demonstrated that the antibacterial rates of PCOMC-Cur@β-CD film against E. coli and S. aureus reached 92 % and 95 %, respectively, which was attributed to the slow release of Cur when Cur@β-CD was dissolved in PVA material and the Schiff base reaction between Cur and amino groups on CS. These findings indicate that the PCOMC-Cur@β-CD film developed in this work can provide certain insights into the field of food packaging.

PH-tailored delivery of a multitarget anticancer benzimidazole derivative using a PEGylated β-cyclodextrin-curcumin functionalized nanocomplex

In this study, we aimed to enhance the bioavailability of a benzimidazole derivative with potent anticancer potential through a nano-based approach. Benzimidazole-loaded polyethylene glycol-β-cyclodextrin–functionalized curcumin nanocomplex (BMPE-Cur) was prepared and characterized for its physicochemical properties and drug release profiles under different pH conditions. In addition, the biological activities of the nanocomplex including antioxidant potentials and pro-apoptogenic properties, against HepG2, PC3, and the chemo-resistant MCF-7-ADR cell lines relative to the normal Wi-38 cell line were in vitro assessed and compared with those of the free benzimidazole compound. In addition to FTIR, XRD, and NMR spectral studies, a polymeric nanocomplex with an average particle size of 467.7 nm and high stability was successfully developed, as indicated by the negative zeta potential (−28.24 mV). The nanocomplex also showed prolonged pH-sensitive sustained drug release under conditions that replicated the tumor's extra/intracellular pH. The formulated nanocomplex also demonstrated potent radical scavenging capacity owing to the inclusion of curcumin, a known radical quencher. In addition, compared with the free compound, BMPE-Cur induced DNA fragmentation-driven cell cycle arrest in HepG2, PC3, and MCF-7-ADR cells at the G1/S, G1 & S phases; respectively, with remarkable selectivity. In conclusion, the newly formulated BMPE-Cur nanocomplex represents an attractive multitarget anticancer candidate.

Preparation of curcumin-loaded chitosan/polyvinyl alcohol intelligent active films for food packaging and freshness monitoring

To fulfill the current need for intelligent active food packaging. This study incorporated the curcumin inclusion complexes (CUR-CD) into chitosan/polyvinyl alcohol polymer to develop a new intelligent active film. The structures of films were analyzed by Fourier-transform infrared (FT-IR), scanning electron microscope (SEM), and so on. The CP-Cur150 film displays exceptional mechanical properties, water vapor barrier, and UV blocking capabilities as demonstrated by physical analysis. The CP-Cur150 film exhibited free radical scavenging rates on 2,2-diazo-di-3-ethylbenzothiazolin-6-sulfonic (ABTS) (98 %) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (87 %). Additionally, it showed inhibitory effects on Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli), reducing live colony counts by approximately 2.7 and 1.3 Log10 CFU/mL, respectively. The films were used to monitor the shrimp's freshness in real time. With the spoilage of shrimp, the film exhibited clear color fluctuations, from light yellow to red. In addition, the evaluation of the impact of films on pork pH, total volatile basic nitrogen, and total bacterial counts demonstrated that the CP-Cur150 film displayed the most significant effectiveness in preserving freshness, thereby extending the shelf life of pork.

Ultrasonication-based preparation of raw chitin nanofiber and evaluation of its reinforcement effect on chitosan film for functionalization with curcumin

Raw chitin nanofibers (CNF) through an 8-min high-power sonication process were successfully prepared. Well-preserved nanofibers with diameters of 50–100 nm and lengths of 3–10 μm were observed by atomic force microscopy. The high aspect ratio induced strong entanglement under high shear rates, leading to a typical nanofiber four-region shear-thinning behavior. These native chitin nanofibers were incorporated into chitosan film-forming solutions at different concentrations. FTIR results indicated intermolecular hydrogen bonds between CS and CNF. Tensile strength was significantly enhanced with the inclusion of CNF, with the highest stress at 30% CNF incorporation. Elongation at break increased up to 35% with 10% CNF concentration, gradually decreasing with higher CNF content. CS with 20% addition of CNF content was chosen for curcumin addition considering the high mechanical performance and low grammage value. Oxygen transmission rate reduced slowly with increasing CNF concentration, and a significant drop by about 90% was observed upon curcumin inclusion. UV blocking performance and antioxidant ability were drastically improved after curcumin inclusion. The utilization of raw CNF as a film reinforcement nanofiller offers several advantages, including ease of preparation, higher aspect ratio, longer length, and superior mechanical performance. CNF-reinforced chitosan films emerge as potent candidates for functional food packaging materials.

Assessing the efficacy of curcumin-loaded alginate hydrogel on skin wound healing: A gene expression analysis.

Skin tissue engineering has gained significant attention as a promising alternative to traditional treatments for skin injuries. In this study, we developed 3D hydrogel-based scaffolds, Alginate, incorporating different concentrations of Curcumin and evaluated their properties, including morphology, swelling behavior, weight loss, as well as hemo- and cytocompatibility. Furthermore, we investigated the therapeutic potential of Alginate hydrogel containing different amounts of Curcumin using an in vitro wound healing model. The prepared hydrogels exhibited remarkable characteristics, SEM showed that the pore size of hydrogels was 134.64μm with interconnected pores, making it conducive for cellular infiltration and nutrient exchange. Moreover, hydrogels demonstrated excellent biodegradability, losing 63.5% of its weight over 14 days. In addition, the prepared hydrogels had a stable release of curcumin for 3days. The results also show the hemocompatibility of prepared hydrogels and a low amount of blood clotting. To assess the efficacy of the developed hydrogels, 3T3 fibroblast growth was examined during various incubation times. The results indicated that the inclusion of Curcumin at a concentration of 0.1mg/mL positively influenced cellular behavior. The animal study showed that Alginate hydrogel containing 0.1mg/mL curcumin had high wound closure(more than 80%) after 14 days. In addition, it showed up-regulation of essential wound healing genes, including TGFβ1 and VEGF, promoting tissue repair and angiogenesis. Furthermore, the treated group exhibited down-regulation of MMP9 gene expression, indicating a reduction in matrix degradation and inflammation. The observed cellular responses and gene expression changes substantiate the therapeutic efficacy of prepared hydrogels. Consequently, our study showed the healing effect of alginate-based hydrogel containing Curcumin on skin injuries.

Self-assembly embedding of curcumin by alkylated rice bran protein

Lysine-rich rice bran protein (RBP) can be used as raw material for alkylation modification to improve the self-assembly performance of protein. The results of 1H NMR, degree of alkylation, and DSC analysis showed that the alkyl chain was successfully attached to the RBP. The surface hydrophobicity and absolute ζ-potential increased. The three-dimensional structure of the alkylated RBP (ARBP) become more porous and ARBP-2 was selected as the material for embedding curcumin. The XRD results revealed that curcumin induced self-aggregation of ARBP-2 and the inclusion of curcumin was attained. The maximum encapsulation efficiency of curcumin was 82.67 % and the maximum loading amount was 171.37 g/100 g RBP. The results of atomic force microscopy (AFM), particle size, and polydispersity index (PDI) analyses revealed that the particles in the system were aggregated after curcumin was added. Curcumin was well protected by encapsulation in the self-assembled particles. Thus, this study provides a new strategy for the embedding and delivery of curcumin.

Effect of Curcumin Consumption on Inflammation and Oxidative Stress in Patients on Hemodialysis: A Literature Review.

Advanced chronic kidney disease (CKD) stages lead to exacerbated inflammation and oxidative stress. Patients with CKD in stage 5 need renal hemodialysis (HD) to remove toxins and waste products. However, this renal replacement therapy is inefficient in controlling inflammation. Regular curcumin consumption has been shown to reduce inflammation and oxidative stress in subjects with chronic pathologies, suggesting that the daily intake of curcumin may alleviate these conditions in HD patients. This review analyzes the available scientific evidence regarding the effect of curcumin intake on oxidative stress and inflammation in HD patients, focusing on the mechanisms and consequences of HD and curcumin consumption. The inclusion of curcumin as a dietary therapeutic supplement in HD patients has shown to control the inflammation status. However, the optimal dose and oral vehicle for curcumin administration are yet to be determined. It is important to consider studies on curcumin bioaccessibility to design effective oral administration vehicles. This information will contribute to the achievement of future nutritional interventions that validate the efficacy of curcumin supplementation as part of diet therapy in HD.

Curcumin-loaded microemulsion: formulation, characterization, and in vitro skin penetration

Objective Formulation of curcumin in a microemulsion with a high loading capacity and that favors its penetration into the skin. Significance Take advantage of the properties of microemulsions to promote the penetration of curcumin into the skin, with the aim of enhancing its therapeutic effects. Methods Curcumin was formulated in microemulsions based on oleic acid (oil phase), Tween® 80 (surfactant), and Transcutol® HP (cosurfactant). The microemulsion formation area was mapped by constructing pseudo-ternary diagrams for surfactant:co-surfactant ratios 1:1, 1:2, and 2:1. Microemulsions were characterized through measurements of specific weight, refractive index, conductivity, viscosity, droplet size, and in vitro skin permeation studies. Results Nine microemulsions were prepared and characterized, showing clear, stable formulations with globule size dependent on the proportion of the components. The microemulsion with the highest loading capacity (60 mg/mL), based on Tween® 80, Transcutol® HP, oleic acid, and water (40:40:10:10) was able to penetrate the viable epidermis, finding a total amount of curcumin in the receptor medium at 24 h of 10.17 ± 9.7 µg/cm2. The distribution of curcumin in the skin, visualized by confocal laser scanning microscopy, showed that the maximum amount was located between 20 and 30 µm. Conclusion The inclusion of curcumin in a microemulsion allows its passage into and through the skin. The localization of curcumin, especially in the viable epidermis, would be important for those cases where local conditions are sought to be treated.

Impact of dietary curcumin administration along with feed-born silver nanoparticle on growth, hemato-biochemical parameters, and digestive enzyme activity of common carp (Cyprinus carpio).

This research explored the impacts of feed-born silver nanoparticles (AgNPs) on common carp (Cyprinus carpio) and whether dietary curcumin supplementation could ameliorate the impacts of AgNPs on growth, hemato-biochemical parameters and digestive enzyme activity. Nine experimental diets were prepared containing 0.00, 0.05, and 0.15 g kg-1 AgNPs, as well as 0.00, 0.75, and 1.50 g kg-1 curcumin in a factorial design. Triplicate groups of common carp (4.82 ± 0.41 g) were fed on the test diets for 60 days. The results demonstrated that AgNPs reduced growth performance and enhanced the feed conversion ratio dose-dependently. Supplementing 0.75 g kg-1 curcumin at a low AgNP level improved the growth rate, while its inclusion at a high AgNP level led to further suppression of growth performance. The highest hematocrit value, hemoglobin concentration and white blood cell count were recorded in the group receiving 0.75 g kg-1 curcumin. Serum glucose, cholesterol and triglyceride concentrations were elevated by increasing AgNP levels. However, curcumin inclusion, particularly at the lower level of AgNPs significantly decreased their values. Similarly, intestinal alkaline protease and lipase activities were progressively reduced by increasing dietary AgNP contents, but, significant improvements were observed by curcumin application at the lower AgNP level. Our results revealed that curcumin supplementation could limit the toxic effects of lower dietary AgNP contents.

Inclusion of curcumin in b-cyclodextrin: a promising prospective as food ingredient

In the present work, the inclusion complexes between curcumin (CC) and β-cyclodextrin (β-CD) were obtained through co-precipitation at different times of magnetic stirring and simple mixing. The stoichiometric ratio between CC and β-CD was ascertained to be 1:2. The samples prepared by co-precipitation (24 h magnetic stirring and 24 h cold static precipitation) and by simple mixing, showed the best performance in terms of powder recovery (72 and 97%, respectively), encapsulation efficiency (72 and 95%, respectively) and tinting power, while no significant differences were revealed in terms of loading capacity (about 14%). Using either inclusion method, the complexation of CC into β-CD make the colorant 20-fold more water soluble than pure CC. The pigment stability was also enhanced under different storage conditions (high temperature and light exposure), either when applied in liquid or gel state. However, after 30 days under natural light exposure, an appreciable colour change from yellow to orange was revealed to an extent probably perceptible by an inexperienced observer.

Curcumin or microencapsulated phytogenic blend to replace ionophore and non-ionophore antibiotics in weaned calves: Effects on growth performance and health

The objective was to determine whether the inclusion of curcumin and microencapsulated phytogenic (alone or combined) could replace the combination of ionophore and non-ionophore antibiotics in the diet of calves in the initial breeding phase and maintain zootechnical performance. The microencapsulated phytogenic blend used in this study contained carvacrol, cinnamaldehyde, and thymol. Twenty-four calves housed in individual pens were divided into the following groups: CONTROL (a diet containing 0.81 mg/kg of body weight (BW) of monensin and 0.162 mg/kg of BW of flavomycin), CUR (a diet containing 0.9 mg/kg of BW of curcumin), PHYT (a diet containing 2.7 mg/kg of BW of phytogenic) and CUR + PHYT (a diet containing 0.9 mg/kg of BW of curcumin and 2.7 mg/kg of BW of phytogenic). Calves in the CONTROL and PHYT groups showed greater final BW than animals in the CURC group (P = 0.05) due to the greater daily weight gain during the experimental period (P = 0.05). The calves in the CURC, PHYT, and CURC + PHYT groups had lower leukocyte counts than the CONTROL group (P = 0.05), and calves in the PHYT and CURC + PHYT groups had lower lymphocyte counts than the CONTROL (P = 0.05). The CURC treatment provided higher concentrations of IgA than the CONTROL and PHYT groups (P = 0.03); calves in the CURC group had higher concentrations of light chain IgG than the other treatments (P < 0.01). These findings suggest that a microencapsulated phytogenic agent containing carvacrol, cinnamaldehyde, and thymol can replace a combination of ionophore (monensin) and non-ionophore (flavomycin) antibiotics in the diet of dairy calves during the post-weaning period and that further research should be carried out to identify a better curcumin dosage for this animal category.

Dietary curcumin supplementation regulates the lipid metabolism in laying hens

This study was conducted to investigate the effects of dietary curcumin on the hepatic lipid metabolism and antioxidant capacity in Roman laying hens. A total of 144 Roman laying hens (28 weeks old) were allocated 4 dietary treatments that included curcumin at concentrations of 0, 50, 150, and 250 mg/kg for 9 weeks. Each treatment had 6 replicates per group with 6 hens per replicate (2 birds/cage). The results showed that dietary supplementation of curcumin decreased (P < 0.05) the absolute and relative abdominal fat weight during 32–34 wk. Curcumin supplemented at 150 mg/kg had been reduced (P < 0.05) the concentrations of triglycerides (TG) and total cholesterol in plasma, the liver TG concentrations, and the enzyme activities of malic (ME) and lipoprotein lipase of Roman laying hens throughout the experiment. Curcumin supplemented also decreased (P < 0.05) the mRNA expression of Sterol expression-regulatory protein-1c (SREBP-1c), ACC, FAS, ME and while it increased the mRNA expression of Apolipoprotein B-100 and Apolipoprotein-II. Further research found that dietary curcumin boosted the activity of the antioxidant enzymes Superoxide dismutase, Catalase, and Glutathione peroxidase in plasma and liver. It lowered the levels of Malondialdehyde in plasma and liver. In conclusion, curcumin inclusion regulated the hepatic fat metabolism, reduced abdominal fat deposition and improved the antioxidant capacity of Roman laying hens. Highlights Curcumin can regulate the lipid metabolism of Roman laying hens by reducing the activity of lipid metabolism enzymes in the liver. Curcumin can regulate the fat deposition of Roman laying hens by reducing the expression of genes involved in lipid synthesis in the liver.

Preparation and characterization of polylactic acid/fenugreek essential oil/curcumin composite films for food packaging applications

Curcumin and Fenugreek essential oil (FEO) were blended into the PLA matrix by solution casting technique to improve the functional properties of the composite film. Both fillers (curcumin and FEO) were properly combined and uniformly distributed in the polymer matrix to create a PLA-compatible composite evidenced by Scanning electron microscope (SEM) and Fourier Transform Infrared (FT-IR) results. The addition of FEO and curcumin to the composite film improved UV-blocking, surface color, tensile strength, flexibility, thickness, and Water contact angle (WCA). However, the inclusion of curcumin and FEO slightly diminish the Water vapor permeability (WVP) while maintaining its thermal stability. The PLA-based composite film exhibited good antibacterial and anti-oxidant properties. In addition, a food quality test was performed on strawberry, and the results were compared to the commercial (polyethylene) film.

Development and investigation of antibacterial and antioxidant characteristics of poly lactic acid films blended with neem oil and curcumin

AbstractAntibacterial films based on polylactic acid (PLA) were developed by blended with neem oil (NO) and curcumin via solvent casting technique. Nuclear magnetic resonance (NMR) results revealed the presence of fillers (NO and curcumin) in the PLA matrix. Without losing much of the PLA film's transparency, the composite film has exceptional ultra violet‐blocking properties. The addition of NO to the PLA matrix increased the elongation of the composite film by reducing tensile strength while not affect their thermal stability. The water vapor transfer rate (WVTR), water contact angle (WCA), and oxygen transmission rate (OTR) of the film were marginally improved by the introduction of NO and curcumin. The curcumin inclusion prevented the plasticized PLA/NO/curcumin compositions surfaces from porosity according to the morphology studies. Further, the PLA/NO/curcumin composites films exhibited good antibacterial, antioxidant activity and therefore have the possibility to use as an active packaging material for food preservation and safety.

The Impact of Dietary Curcumin on the Growth Performance, Intestinal Antibacterial Capacity, and Haemato-Biochemical Parameters of Gilthead Seabream (Sparus aurata)

Simple SummaryIn aquaculture, dietary curcumin has been shown to enhance the growth rate, antioxidative status, immunity, and disease resistance of several finfish species. Nevertheless, the potential role of curcumin has not been evaluated in Gilthead seabream as yet. Herein, we tested the effect of dietary curcumin on the growth performance, intestinal antibacterial capacity, and haemato-biochemical parameters of Gilthead seabream. Curcumin was mixed with the basal diet at rates of 0, 1.5, 2, 2.5, and 3%, then fed to the fish for 150 days. The results indicated marked improvements in the growth performance, feed efficiency, and antibacterial capacity of the fish. Further, curcumin enhanced the hematological indices and regulated the biochemical blood metabolites of Gilthead seabream. Dietary curcumin is recommended at a rate of 2–3% to improve the performance of Gilthead seabream.The need to replace antibiotics in aquafeed is increasing, and alternative safe substances are now encouraged for sustainable aquaculture activity. Curcumin is regarded as a multifunctional feed additive with growth-promoting and immunostimulant potential. Thus, this study evaluated dietary inclusion of curcumin at rates of 0, 1.5, 2, 2.5, and 3% in the diets of Gilthead seabream for 150 days. The results showed an improved final body weight, weight gain, specific growth rate, and feed conversion ratio in fish treated with curcumin, in a dose-dependent manner. The highest growth performance was observed in fish fed a diet supplemented with 3% curcumin. The results also showed lowered activity of pathogenic bacteria (Vibrio spp. and Faecal coliform) in the intestines of Gilthead seabream fed a diet with curcumin inclusion, in a dose-dependent manner. The hematological indices were within the normal range for healthy fish, without meaningful effects except for hematocrit, hemoglobin, red blood cells (RBCs), and white blood cells (WBCs), which were markedly increased by dietary curcumin. Phagocytic activity was obviously enhanced by dietary curcumin, compared with the control. The biochemical blood metabolites related to liver function (alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT)), renal tissue (urea), and total cholesterol were within the normal values, without significant differences. Overall, the inclusion of curcumin at a rate of 2–3% improved the growth performance and well-being of Gilthead seabream.

Dietary Curcumin Promotes Gilthead Seabream Larvae Digestive Capacity and Modulates Oxidative Status.

Simple SummaryThe production of marine fish larvae is still recognized to have high mortality rates, so to achieve a more sustainable and competitive aquaculture industry it is essential to develop high-quality larvae. Therefore, this work evaluates if dietary supplementation of natural antioxidant curcumin enhances fish larvae robustness, hence improving growth performance and health status of the farmed fish. For that, two doses of curcumin were assessed and showed that curcumin can modulate larvae condition, digestive capacity, and antioxidant status throughout development. These results bring new perceptions of the effects of dietary curcumin in marine fish larvae that until now have not been studied. Moreover, this data suggested that curcumin can be a suitable feed additive contributing to the development and optimization of microdiets for fish larvae.The larval stage is highly prone to stress due to the ontogenetic and metabolic alterations occurring in fish. Curcumin inclusion in diets has been shown to improve growth by modulating oxidative status, immune response, and/or feed digestibility in several fish species. The aim of the present work was to assess if dietary curcumin could promote marine fish larvae digestive maturation and improve robustness. Gilthead seabream larvae were fed a diet supplemented with curcumin at dose of 0 (CTRL), 1.5 (LOW), or 3.0 g/Kg feed for 27 days. From 4 to 24 days after hatching (DAH), no differences were observed in growth performance. At the end of the experiment (31 DAH) LOW larvae had a better condition factor than CTRL fish. Moreover, HIGH larvae showed higher trypsin and chymotrypsin activity when compared to CTRL fish. LOW and HIGH larvae were able to maintain the mitochondrial reactive oxygen species production during development, in contrast to CTRL larvae. In conclusion, curcumin supplementation seems to promote larvae digestive capacity and modulate the oxidative status during ontogeny. Furthermore, the present results provide new insights on the impacts of dietary antioxidants on marine larvae development and a possible improvement of robustness in the short and long term.

Dietary curcumin decreased lipid oxidation and enhanced the myofibrillar protein structure of the duck (Anas Platyrhynchos) breast muscle when subjected to storage

Effects of dietary curcumin given to ducks on chemical composition, lipid and protein oxidation and changes of the protein structural properties of the duck breast muscle when subjected to storage (7-day at −18 °C then 12 h at 4 °C) were investigated. A total of 600 1-day-old male ducks were randomly assigned into four groups: the control group (T0, 0 mg/kg dietary curcumin) and curcumin groups (T300, 300 mg/kg; T400, 400 mg/kg and T500, 500 mg/kg dietary curcumin). On the day 70 of age, six ducks were selected and slaughtered and ducks breast muscle were stored at −18 °C then 12 h at 4 °C in each group. There were no significant effects of dietary curcumin on the chemical composition of the duck meat. Dietary curcumin significantly decreased the PV value, TBARS value and carbonyls group content, whereas enhanced the structure (free amino group, secondary structure) and decreased the fluorescence spectroscopy of the protein of the duck breast muscle when subjected to storage. In conclusion, dietary curcumin given to ducks decreased the lipid and protein oxidation, enhanced protein structure of the duck breast muscle when subjected to storage, whereas the dietary curcumin inclusion of 400–500 mg/kg would be more efficient.

COMPARATIVE STUDY ON EFFECT OF TURMERIC POWDER, PROBIOTIC AND PREBIOTIC SUPPLEMENTATION ON BROILER PERFORMANCE AND IMMUNITY

Emerging role of Probiotic, Prebiotic and herbal additives in poultry industry should be considered to overcome abuse of antibiotics. The present research accomplished to scrutinize effectiveness of Curcumin, Probiotic and Prebiotic in broilers productivity. Body weight/gain, feed intake, conversion efficacy, hematological analysis and immune-assay were evaluated. 150 chicks were divided into 5 groups, 3 supplemented separately with the mentioned additives in the feed for 5 weeks and the left were fed on basal diet without additives, 4 groups were vaccinated against avian influenza and infectious bursitis. Results exhibited that Curcumin Probiotic and Prebiotic boosted body gain significantly (p < 0.05), at the end of the study 1948.83g, 1950.83g and 1914.03g, respectively, comparing with control 1750.60g and 1772.20g for vaccinated group. Curcumin and Probiotic supplemented groups presented higher packed cell volume (34.09%, 34.08%), red blood cells count (2.49 106/µl, 2.50 106/µl), white blood cells count (31.95 103/µl, 31.91 103/µl) and hemoglobin (8.78g/dl, 8.78g/dl) than Prebiotic (32.69%, 2.40 106/µl, 31.45 103/µl, 8.48dl) and other groups at 3 weeks old. Moreover, ELISA showed that both Curcumin and Probiotic statistically had highest antibody titer for avian influenza (1606.30 and 1592.83), while probiotic had the highest titer 2374.73 for infectious bursitis. Results of current study concluded that the inclusion of Curcumin, Probiotic and Prebiotic not only advanced performance parameters but also improved hematological indices and immunological reaction of broilers. It is advisable to evaluate the cost efficacy to compromise these results.

Ideal 1:1 stoichiometry curcumin inclusion complex induced potential apoptosisin colorectal adenocarcinoma cells: The clear image of necrosis and functionalized dying effect

Background: This study investigates the inclusion complex of curcumin (CMN) enhance the solubility, which can be utilized for the treatment of colorectal cancer (CRC) greater to free CMN. Methods: CMN solid dispersion (SD) prepared by a hot melt method using CMN with several carriers of poloxamers (P-407 and P-188), gelucire 50/13 (GLR) and mannitol (MNT). Prior, molecular modelling and phase solubility studieswere performed with drug and carriers. The SD characterized by in vitrodrug release, SEM and functionalize dyeing test. Additionally, the cytotoxicity and image of apoptosisresolved to utilize the colorectal adenocarcinoma cell lines. Results: The result showed that CMN-P-407 inclusion complex produced significant properties towards solubility (318 ± 14.46 fold) and dissolution (91 ± 0.431% at 30 min). Similarly, these data fit with insilico model. The IC50 value for inclusion complex found to be 74 and 52 µM/mL, while that for free CMN ranged from 146 and 116 µM/mL on the SW480 and Caco-2 cells respectively. Apoptosis study described that the cells are undergoing cell death by apoptosis and the small number of necrosis. Conclusion: The profound efficiency of CMN-P-407 SD indicated its potential application for CRC treatment by showing a higher capability of inhibiting cell growth compared to that of free CMN.

Treatment of metastatic lung cancer via inhalation administration of curcumin composite particles based on mesoporous silica

Curcumin attracted attention due to its promising anti-cancer properties and safety performance. However, its poor aqueous solubility and low bioavailability have to be overcome before it goes into clinic use. Here, porous composite particles are prepared by loading curcumin into mesoporous material SBA-15, and its therapeutic effect on lung cancer via inhalation administration have also been evaluated. The inclusion of curcumin in host material SBA-15 was confirmed by the reduced surface area and pore diameter of the composite material, and the aerodynamic performance of the composite material was investigated by FT-4 and NGI. Phagocytosis experiments on RAW264.7, the toxicity of material extracts on BEAS-2B cells, and the haemolysis experiments showed that the mesoporous materials had good biocompatibility at 10–400 μg/mL. The B16F10 melanoma metastatic lung mouse model was used to investigate the therapeutic effect of lung cancer after inhalable administration. It was found that the body weight of the curcumin composite particle-administered group decreased more slowly and the lung disease developed slower than the curcumin crude drug group, indicating that the composite particles has a certain inhibitory effect on tumours.