In an era marked by evolving cybersecurity threats, understanding the interplay between Security Awareness Programs, Behavioral Patterns, and Cybersecurity Culture is essential for safeguarding organizational assets and maintaining trust in financial institutions. This study investigated this relationship within Nigeria’s Deposit Money Banks, employing a comprehensive analysis of key variables such as Compliance Adherence, Security Policy Acknowledgements, Training Completion Rate, Risk Awareness and Management, Behavioral Analytics, Employee Feedback and Engagement, and Incident Response Time.Utilizing data gathered from a field survey, the study employed multiple regression analysis and tested for hypotheses. Specifically, the study revealed that Compliance Adherence, Training Completion Rate, and Risk Awareness and Management significantly influenced Employee Feedback and Engagement. Compliance Adherence increased Employee Feedback and Engagement by 0.065 (t-stat: 0.750, p = 0.014), Training Completion Rate by 0.397 (t-stat: 3.846, p < 0.001), and Risk Awareness and Management by 0.237 (t-stat: 2.031, p = 0.028). Similarly, Compliance Adherence, Security Policy Acknowledgements, Training Completion Rate, and Risk Awareness and Management significantly impacted Incident Response Time. Compliance Adherence increased Incident Response Time by 0.067 (t-stat: 0.721, p = 0.041), Security Policy Acknowledgements by 0.087 (t-stat: 0.911, p = 0.033), and Training Completion Rate by 0.188 (t-stat: 1.118, p < 0.001). However, Risk Awareness and Management didn’t show significant impact on Incident Response Time. Moreover, Compliance Adherence, Security Policy Acknowledgements, Training Completion Rate, and Risk Awareness and Management significantly contributed to Cybersecurity Culture. Compliance Adherence increased Cybersecurity Culture by 0.523 (t-stat: 0.101, p < 0.001), Security Policy Acknowledgements by 0.041 (t-stat: 0.279, p = 0.011), Training Completion Rate by 0.188 (t-stat: 1.139, p < 0.001), and Risk Awareness and Management by 0.239 (t-stat: 1.453, p = 0.041). These findings offered valuable insights for policymakers, bank management, cybersecurity professionals, and employees, informing the development of effective cybersecurity strategies and risk management policies. By understanding the intricate relationship between Security Awareness Programs, Behavioral Patterns, and Cybersecurity Culture, stakeholders could better navigate the complexities of the cybersecurity landscape and safeguard organizational interests.