Incidence Of Bacterial Vaginosis Research Articles

Determinants of Bacterial Vaginosis: A Retrospective Study

Background: Among women who can have children, bacterial vaginosis (BV) is the most common cause ofabnormal vaginal discharge. Due to its high prevalence and morbidity, it is crucial to prevent BV by addressing its risk factors. Objective: This study aimed to determine the incidence and determinants of BV in the sexually transmitted infection (STI) division of the Dermatovenereology Department at Dr. Soetomo Hospital in Surabaya. Methods: This retrospective analytical observational study was conducted using a cross-sectional approach. Electronic data from 56 patients were included in the study, covering the period from 2019 to 2022. The patients were divided into two groups: BV patients and controls. BV patients were diagnosed with BV by fulfilling three out of four Amsel criteria, while controls with typical vaginal discharge. Results: The study included 34 BV patients and 22 control patients. Bivariate analysis showed a significant correlation between the incidence of BV and the number of sexual partners (p = 0.009) and the use of intrauterine devices (IUD) (p = 0.002). Multivariate analysis confirmed that both factors were determinants of BV incidence, with IUD use being the most dominant determinant (95% CI 1.86~135.02, p = 0.01). Conclusion: The study found that the use of IUDs and the number of sexual partners significantly determined BV incidence.

Investigating Microbial Dynamics and Bacterial Vaginosis Susceptibility in Women who have Sex with Women

Bacterial Vaginosis (BV) remains a prevalent gynecologic health concern, with emerging research indicating a potential association between women who have sex with women (WSW) and an increased susceptibility to BV infections. Recent studies have explored the microbial dynamics within the vaginal microbiota of WSW, revealing distinct patterns that may contribute to a higher incidence of BV in this population. The etiological factors underlying this susceptibility include disruptions in the balance of Lactobacillus species, crucial for maintaining vaginal health. However, the existing literature presents a complex interplay of socio-behavioral and biological factors influencing BV risk among WSW, necessitating further investigation. Future research should strive to gain a more comprehensive understanding of the specific mechanisms influencing microbial communities in the context of same-sex sexual activity among women, exploring potential preventive strategies and tailored interventions to mitigate BV risks in this population. Our review highlights the need for a refined and multidisciplinary approach to address the intricacies of BV susceptibility in women engaging in same-sex relationships.

Association between serum carotenoids and bacterial vaginosis infection among American women.

Bacterial vaginosis (BV) is a widely occurring vaginal inflammation in women of childbearing age caused by dysbiosis of the vaginal flora. Few studies have investigated the effect of serum carotenoids on the development and pathogenesis of BV. This study thus aimed to explore the correlation between serum carotenoids and BV in American women. The analysis included 1252 participants with BV from the National Health and Nutrition Examination Survey (NHANES) between 2001 and 2004. Multiple logistic regression was conducted to explore the correlation between BV and serum carotenoids, while smooth curve fitting was utilized to examine potential nonlinear correlations. Furthermore, stratified subgroup analyses and sensitivity analyses were conducted. ORs reflected the correlation between BV and serum carotenoids. Results of multiple logistic regression indicated that total serum carotenoids and BV had an inverse correlation. In the fully adjusted model II, the quartile with the highest levels of α-carotene and β-cryptoxanthin had a substantially lower incidence of BV. Smooth curve fitting revealed a significant negative linear correlation between serum carotenoids and the incidence of BV. The negative correlation between serum carotenoids and BV was relatively stable in stratified analyses. Moreover, in sensitivity analyses, the association between serum carotenoids and BV persisted, and β-carotene became significantly negatively correlated with BV. This study found an inverse correlation between serum carotenoids and the prevalence of BV.

Association between body mass index and prevalence of bacterial vaginosis: Results from the NHANES 2001-2004 study.

The impact of bacterial vaginosis on women's health is an increasing concern; however, the effect of the obesity index on bacterial vaginosis is controversial. We investigated the association between body mass index and bacterial vaginosis in women in the United States. This was a cross-sectional study which obtained the data from the National Health and Nutrition Examination Survey from 2001 to 2004, in which weighted multivariate regression and logistic regression analyses were performed to explore the independent relationship between body mass index and bacterial vaginosis. Subgroup analyses and smoothed curve fitting were also performed. A total of 5,428 participants were enrolled, and the findings show that the participants with higher body mass index tended to have a higher incidence of bacterial vaginosis. In the fully adjusted model, a positive association between bacterial vaginosis and body mass index was observed (Odd's ratio = 1.03, 95% Confidence interval, 1.01-1.04). The subgroup analysis showed that this positive association was significant in non-Hispanic White individuals (Odd's ratio = 1.0327, 95% Confidence interval, 1.0163, 1.0493). Increased bacterial vaginosis positivity may be associated with an increased body mass index.

Low prevalence of bacterial vaginosis in Kenyan adolescent girls and rapid incidence after first sex

Bacterial vaginosis (BV) is a risk factor for sexually transmitted infections (STI) including HIV. Adult African women have high prevalence of BV, but it is not known when first BV occurs. We sought to describe BV in younger African women, before and after first sex, and to determine incidence of BV and significant correlates of BV incidence and recurrence. In a prospective observational cohort study enrolling adolescents with limited sexual experience, young women ages 16-21 were recruited in Thika, Kenya. Eligible participants were HIV and HSV-2 seronegative and reported zero or one lifetime sexual partner. Nugent score was determined at quarterly visits from vaginal Gram stains. Trends in BV were described over time; hazard ratios were calculated using Cox regression and relative risk of BV was estimated using generalized estimating equations and Poisson regression. We enrolled 400 participants with a median age of 18.6 years (IQR 16-21). The majority (322, 80.5%) reported no history of sex, while 78 (19.5%) reported sex with 1 partner. At enrollment, BV (Nugent score ≥7) was uncommon (21 of 375, 5.6%). Overall, 144 participants had BV at least once, for an incidence rate of 16.5 cases per 100 person-years. Prior to first sex, BV was present at 2.8% of visits, compared to 13.7% of visits post-first sex. An adjusted model of BV incidence observed that first sex was associated with more than twofold increased BV risk (aHR 2.44, p=0.009, CI 1.25-4.76). Chlamydia diagnosis (aHR 1.73, p=0.02, CI 1.1-2.8) and HSV-2 seropositivity (aHR 2.88, p=0.021, CI 1.17-7.09) were both associated with incident BV. A multivariate GEE model including all episodes of BV demonstrated risk factors including first sex, STIs, urban residence, recent sex and no income; the most important risk factor was first sex (aRR 1.92, p=0.018, CI 1.12-3.31). Probability of BV increased with each subsequent episode; mean Nugent scores increased after each BV episode. Using detailed longitudinal observation, we found that Kenyan adolescents have almost no BV prior to first sex, and that initiation of sexual activity was the strongest risk factor for both prevalent and incident BV.

Stage 2: The Vaginal Flora in Women Undergoing Fetal Spina Bifida Repair and Its Potential Association with Preterm Rupture of Membranes and Preterm Birth.

Vaginal dysbiosis affects pregnancy outcomes, however, the relevance of abnormal findings on pre/post-surgical vaginal culture in women undergoing fetal spina bifida (fSB) repair is unknown. To describe the incidence of normal and abnormal pre- and post-surgical vaginal microorganisms in fSB patients and to investigate potential associations between the type of vaginal flora and the occurrence of preterm prelabour rupture of membranes (PPROM) and preterm birth (PTB). 99 women undergoing fSB repair were eligible (2010-2019). Pre-surgical vaginal culture was routinely taken before surgery. Post-surgical cultures were taken on indication. Vaginal flora was categorized into four categories: healthy vaginal flora (HVF), bacterial vaginosis (BV), desquamative inflammatory vaginitis (DIV), and yeast infection. The incidence of HVF, BV, DIV, or yeast infections was not statistically different between the pre- and postoperative patients. Furthermore, an abnormal pre/post-surgical vaginal flora was not associated with PPROM (OR 1.57 (0.74-3.32), p = 0.213)/OR 1.26 (0.62-2.55), p = 0.515), or with PTB (OR 1.19 (0.82-1.73), p = 0.315)/(OR 0.86 (0.60-1.24), p = 0.425). Abnormal vaginal microbiome was not associated with PPROM and PTB when appropriate treatment was performed.

MICROBIOLOGICAL PROFILE IN WOMEN WITH SYMPTOMATIC VAGINAL DISCHARGE

The study's main goals are to determine the prevalence of symptomatic vaginal discharge in different age groups and the microbiological makeup of women who report with symptomatic vaginal discharge. The study was conducted in 200 cases. A detailed clinical history and examination of all cases were done. A speculum examination done to visualize cervix and vagina , swabs were collected from the posterior fornix of vagina and sent for microbiological examination. Following tests were done a) Gram staining and Nugent scoring system used, based on the morphology of the bacteria, score >/= to 7 is considered positive for bacterial vaginosis, b) Wet mount preparation done to look for motility of Trichomonas, c) KOH preparation, d) Swabs are inoculated in Blood agar, Mac Conkey's agar and Sabouraud's dextrose agar. Maximum patients had associated symptoms as foul smell (27%),followed by itching(26%) ,others like abdominal pain(11.5%),burning micturition(6%) , heavy menstrual bleeding(4.5%) and low backache(2.5%). Majority had homogeneous discharge(44.5%), followed by mucopurulent(19.5%),curdy white(18%) and yellowish (18%). Incidence of bacterial vaginosis is maximum(42.5%), followed by vaginal candidiasis (21.5%),other non specific (17%) and Trichomonas(8%). In around 11% of cases no cause was identified.

Bacterial vaginosis, vulvovaginal candidiasis, and trichomonal vaginitis in reproductive-age women in Yunnan, China: a descriptive study

This study aimed to determine the prevalence of and risk factors associated with BV(bacterial vaginosis, BV), VVC (vulvovaginal candidiasis, VVC) and TV (trichomonal vaginitis, TV) among non-pregnant women. Among 770 women included in analyses, surveyed using a questionnaire and subsequently diagnosed with BV, VVC and TV via Gram staining and vaginal swab microscopy. Vaginal infections were prevalent in 31.30%, with BV being the most prevalent (21.35%). Single-variable analysis revealed that an age of 20–29 years (odds ratio [OR] = 2.31, 95% CI: 1.24–4.29; p = .007) and lack of education (OR = 0.50, 95% CI: 0.28-0.89; p = .018) were significantly associated with BV. However, an age of 30–39 years was significantly associated with VVC (OR = 2.12, 95% CI: 1.03–4.38; p = .038). Multivariable analysis confirmed that miscarriage was an independent predictor of BV and VVC. Miscarriage was significantly associated with the incidence of BV and VVC (OR = 1.680, 95% CI: 1.146–2.462; p = .011 and OR = 2.04, 95% CI: 1.30–3.20; p = .002, respectively). In conclusion, BV appears to be the predominant cause of vaginitis, risk factors for vaginitis include age and level of education and miscarriage. IMPACT STATEMENT What is already known on this subject? Inflammation of the vagina, or vaginitis, is caused by various infectious and non-infectious factors. The most common causes of infectious vaginitis are BV, VVC and TV. Kunming located at the southwestern border of China, However, there is still no systematic research investigating the status of vaginitis infection in Yunnan Province. Therefore, the present study aimed to determine the prevalence of these vaginal infections; BV, VVC, and TV, among women of childbearing age, and to assess the prevalence of vaginal infections and the associated risk factors. What do the results of this study add? In our study we found that vaginal infections were prevalent in 31.30% of reproductive-age women, with BV being the most prevalent (21.35%). We believe that our study makes a significant contribution to the literature because we report that BV appears to be the predominant cause of vaginitis, followed by VVC and TV. Risk factors for vaginitis include age, miscarriage and level of education. What are the implications of these findings for clinical practice and/or further research? This study aimed to determine the prevalence of these vaginal infections, BV, VVC and TV, and to assess the prevalence of vaginal infections and the associated risk factors. Health education interventions are recommended to raise women’s awareness of vaginitis and its prevention.

Determining the Prevalence of Bacterial Vaginosis and their Patterns of Susceptibility to Antibiotics among Benghazi Women, Libya

Vaginal discharge in women is occasionally caused by aerobic bacterial organisms. The study aimed to determine the etiology of female vaginosis and their antibiotic sensitivity pattern. HV culture results, age, and sex of all female patients with suspected bacterial vaginosis were collected. High vaginal swabs were inoculated into MacConkey agar, 5% blood agar, and chocolate agar and then incubated at 37°C in the presence of 5% CO2 for 24-48 h. The antimicrobial susceptibility testing was performed by the disk diffusion method. Ten different antibiotic discs were used: Amikacin, Augmentin, Ceftazidime, Ceftriaxone, Ciprofloxacin, Gentamicin, Levofloxacin, Meropenem, Septrin, and Clindamycin. After 24 hours, zones were measured in mm, and zone interpretations were in accordance with the National Committee for Clinical Laboratory Standards criteria guidelines. A total of 215 females were included in the study, the incidence of bacterial vaginosis was 18.6% (40/215). Females between 33 and 45 years old had a somewhat high prevalence (19/40:47.5%) of bacterial vaginosis. The most frequent isolates were 45% (18/40) Escherichia coli followed by 15% (6/40) Strep pneumonia. The in vitro susceptibility tests of the most common isolates showed high resistance levels to commonly used antibiotics such as Augmentin and Gentamycin. Whereas highly sensitive rates were observed for Ceftriaxone 70%, followed by Ciprofloxacin 57.5%. Ceftriaxone and Ciprofloxacin showed the best antibiotic sensitivity. Additional studies are necessary to recognize those bacterial species that cause vaginal infections and determine the susceptibility of those species to recently used antibiotics.

Effects of vaginal microecology and immunity on the pregnancy outcome of cervical cerclage

ObjectiveTo investigate the effects of vaginal microecology and immune status on the pregnancy outcome of cervical cerclage.MethodsThe clinical data of 125 patients with cervical incompetence who underwent transvaginal cervical cerclage in our hospital from January 2018 to January 2021 were collected, based on which the associations of vaginal microecology and related immune cytokines (IL-1β, IL-6, IL-8 and TNF-α) with the cervical cerclage outcome were explored.ResultsAll of the 125 patients had singleton pregnancies, who were aged 20–43 years, with a mean of (32.34 ± 5.17) years. The surgery was successful in 104 patients (full-term delivery or survival of premature infants), while unsuccessful in 21 patients (late miscarriage or death of premature infants), revealing a success rate of 83.20%. There were 70 full-term deliveries, 34 premature deliveries (28 survived while 6 died), and 15 late miscarriages. Univariate analysis revealed statistically significant differences in the timing of surgery, gestational age of cerclage, preoperative cervical canal length, genital tract infections and preoperative invasive procedures between the successful and unsuccessful groups (P < 0.05). Insignificant differences were found in the pre-pregnancy body mass index (BMI) (P > 0.05). According to the multivariate analysis results, cerclage timing and genital tract infections were independent risk factors for postoperative pregnancy failure (P < 0.05). The pathogen detection rates in the two groups of pregnant women were analyzed, finding significantly higher incidence of bacterial vaginosis (BV) in the unsuccessful group than in the successful group (P < 0.05). Inter-group comparison revealed that the positive rates for vaginal microenvironmental factors (LE, NAG, SNA, H2O2 and pH) were all significantly higher in the unsuccessful group than in the successful group (P < 0.05). Besides, the immune cytokine levels in the cervicovaginal secretions were also all significantly higher in the unsuccessful group than in the successful group (P < 0.05).ConclusionThe pregnancy outcome of patients undergoing cervical cerclage is associated with the imbalance of vaginal microecology and the levels of IL-1β, IL-6, IL-8 and TNF-α in cervicovaginal secretions.

Epidemiological aspects of bacterial vaginosis in Republic of Dagestan

Frequency of bacterial vaginosis (BV) in the population of women aged 14–45 is 23.0–29.0%, however, in the structure of infections accompanied by pathological vaginal discharge, it accounts for up to 48.6%. This article presents data on the prevalence of BV in the Republic of Dagestan (RD).Aim. To study the prevalence of bacterial vaginosis in the RD in various ecological zones.Materials and methods. The analysis of the prevalence of BV in various ecological zones of the RD was carried out by processing the results of the examination of patients who applied for gynecological care.Results. The prevalence of bacterial vaginosis in the Republic of Dagestan, according to our study, equals 53.6%.Conclusion. In connection with the high incidence of BV in the Republic of Dagestan, it is necessary to search for new methods of treatment and prevention of this pathology.

Identification of Gardnerella vaginalis by Molecular Methods in Women Diagnosed With Bacterial Vaginosis in Isfahan, Iran

Background: Bacterial vaginosis (BV) is one of the most common causes of abnormal vaginal discharge in women. The disease is characterized by an imbalance in the vaginal bacterial flora. We aimed to determine the frequency of Gardnerella vaginosis by cultivation and molecular method. Methods: In summer 2019, the vaginal secretion of 110 women with BV were collected and isolated for G. vaginalis, in Isfahan. The isolates were identified by the biochemical test. Polymerase chain reaction (PCR) was performed to detect G. vaginalis in vaginal secretions. Antibiotic susceptibility of the isolates was evaluated by disc diffusion method. Results: Gardnerella vaginalis was isolated from five samples among the 110 patients with symptoms of BV by cultivation. Based on molecular identification, G. vaginalis was shown in 32 (29.1%) samples. G. vaginalis isolates were resistant to clindamycin (20%) and amoxicillin/clavulanic acid (80%). All the isolates were sensitive to Metronidazole. All women with this infection were married and most (43.8%) belonged to the 25-30 year-old age group. A significant difference was found between participants with positive clue cell (P&lt;0.01) and pH&gt;4.5 vaginal discharge (P&lt;0.01) in the PCR-positive and the PCR-negative women. Conclusion: High prevalence of Gardnerella vaginalis in women with vaginosis confirms the important role of bacteria in the incidence of BV. Identification of pathogenic agents of G. vaginosis using molecular methods and determining their antibiotic susceptibility pattern is essential for proper treatment in different societies.

RELATIONSHIP OF AGE, MENOPAUSE STATUS, AND BMI WITH THE EVENT OF BACTERIAL VAGINOSIS IN NEW PATIENTS OF PELVIC ORGAN PROLAPSE

AbstractBackground and Purpose : The prevalence of Bacterial Vaginosis (BV) among Asian women in India and Indonesia is 32% while in the case of BV in 2018 the obstetric clinic of Dr. Soetomo Regional Hospital is 181 BV sufferers. The impact of BV on Pelvic Organ Prolapse (POP) patients, namely the delay in POP treatment such as BV pessaries can be influenced by age, menopausal status, and BMI. This study aims to analyze the relationship of age, menopausal status, and BMI with the incidence of BV in POP patients. Methods: This study used observational analytics with a retrospective and cross sectional approach. The number of samples used was 86 patients. Samoel taking technique with total sampling. The independent variable is the incidence of BV, while the dependent variable is age, menopause status, BMI. Bivariate data analysis using chi square, fisher's exact test and multivariate test using multiple logistic regression tests. Results: The results showed a positive BV prevalence of 76.7% with the majority of patients aged over 60 years at 44.2%, having menopause at 81.4%, and having a BMI of 18.5-24.5 at 47.7%. Fisher's exact test analysis test showed p value of maternal age = 0.003 (RR = 6.398) and p value of BMI = 0.767 (p&gt; 0.005). Chi square analysis test showed p value of menopause status = 0.009 (RR = 4.833). The multiple logistic regression analysis test showed that maternal age was the most dominant factor with a value of p = 0.006 (OR = 6.398 (1,711-23,929)). Conclusion: There is a relationship between age and menopausal status with the incidence of BV. Age is the most dominant independent factor in the incidence of BV.

BACTERIAL VAGINOSIS ASSOCIATED WITH THE ABNORMAL BODY MASS INDEX

AbstractBackground: Based on data in RSUD Dr. Soetomo Surabaya, showed that during the 2018 period, bacterial vaginosis was among the top 10 most diseases. One risk factor for bacterial vaginosis is still controversial is the body mass index. The purpose of this study was to determine the association between the incidence of bacterial vaginosis with body mass index in RSUD Dr. Soetomo. Method: The research data used secondary data. This research method was cross sectional with observational analytic research design. The total samples were 158 patients, according to inclusion and exclusion criteria. The sampling technique used purposive sampling. Data analysis used chi square test. Results: The results 158 patients, divided into 79 (50%) patients with BV and 79 (50%) patients without BV. Based on BMI, 88 (55.7%) patients with normal BMI and 70 (44.3%) people with abnormal BMI. Patients with normal BMI were mostly not BV, that was 56 (63.6%) people and 32 (36.4%) people were BV positive. Patients with abnormal BMI were mostly BV, that was 47 (67.1%) people and 23 (32.9%) people did not BV. Statistical analysis showed there was a significant association between the incidence of bacterial vaginosis with body mass index (p= 0.001) with contingency coefficient 0.292. Conclusion: There is associated between the incidence of bacterial vaginosis with body mass index in RSUD Dr. Soetomo in 2017-2018.

Effect of vaginal estrogen in postmenopausal women using vaginal pessary for pelvic organ prolapse treatment: a randomized controlled trial.

Evidence and recommendations for the use of intravaginal estrogen for prevention of bacterial vaginosis and pessary-related complications are limited and controversial. We hypothesized that adding intravaginal estrogen to pessary use would decrease the incidence of bacterial vaginosis and other pessary-related complications. A single-center, open-label, randomized, parallel study was conducted between April 2018 and August 2020. Participants were randomized to either receive intravaginal estriol 0.03mg plus Lactobacillus acidophilus 100 million viable cell vaginal tablets or have no treatment. The Amsel criteria, normal flora index, visual analog scale, Thai version of the ICIQ-VS (International Consultation on Incontinence Questionnaire-Vaginal symptoms) questionnaire, vaginal abrasions and vaginal bleeding were evaluated at entry and at 2- and 14-week follow-up. Seventy-eight women were included and randomized to two groups (39 women per group). At 2-week follow-up, one participant in the intervention group and two participants in the control group were diagnosed with bacterial vaginosis (2.7% vs. 5.7%, p = 0.609). At 14-week follow-up, two participants in the intervention group and two participants in the control group were diagnosed with bacterial vaginosis (5.7% vs. 6.2%, p = 0.926). Normal flora index was significantly different at 2-week follow-up [8 (6.3) vs. 5 (6.0), p = 0.032]. There was no significant difference in the visual analog scale, Thai version of the ICIQ-VS, vaginal abrasions and vaginal bleeding between the 2- and 14-week follow-ups. This study shows no benefit of intravaginal estrogen in reducing bacterial vaginosis, vaginal abrasions, vaginal bleeding and pain in postmenopausal women using a vaginal pessary for pelvic organ prolapse treatment.

Organisms associated with bacterial vaginosis in Nigerian women as determined by PCR-DGGE and 16S rRNA gene sequence.

Bacterial vaginosis (BV) is a condition with diverse etiology. This condition predisposes women to increased susceptibility to sexually transmitted diseases, including human immunodeficiency virus (HIV) infections and preterm birth. The diagnostic methods currently adopted in the evaluation of patient samples for BV are arguably Amsel criteria, and Nugent score that require microscopy and expert interpretation. These two methods are still subjective. The objective of this study was to determine the organisms present in the vagina of 34 HIV negative Nigerian women diagnosed as having bacterial vaginosis by using molecular techniques. The vaginal samples were subjected to DNA extraction, and amplified with eubacterial primers via PCR. The PCR products were separated using denaturing gradient gel electrophoresis (DGGE). Bands were excised, re-amplified, purified and sequenced. Sequence identification was performed using the BLAST algorithm and Genbank data base. Mycoplasma hominis (12/34; 35%) was the most common isolate and 9 (26%) contained one of two clones of an unusual Rainbow Trout intestinal bacterium, while unculturable Streptococcus sp, and other bacteria made up the remaining isolates. The findings indicate further diversity in the etiological agents associated with BV, and raise the question as to whether diagnosis and management of this condition needs to be re-evaluated in countries like Nigeria. There is some controversy over the clinical importance of BV, as it was once regarded as a disease caused by Gardnerella and presenting as an odourous discharge condition, but is now diagnosed without necessarily the presence of these organisms or signs. With the incidence of BV aligned to an increased risk of HIV in a country ravaged by this virus, the effective eradication of BV can only be achieved if appropriate therapies are delivered.

Prevalence of Bacterial Vaginosis and their Antibiotic Susceptibility among Women Attending Different Private Clinics in Tripoli, Libya

Background: Bacterial vaginosis is a worldwide issue due to the raised risk of acquisition of sexually transmitted infections. The aim of the study was to assess the incidence of bacterial vaginosis and their antimicrobial pattern in women attending different private clinics in Tripoli, Libya. Methods: A cross-sectional study was conducted among 204 women between January and October 2020. Gram-stained vaginal swabs were examined microscopically, characterized by colony morphology, and their antimicrobial susceptibility pattern was determined. Results: The overall prevalence of bacterial vaginosis was 41%. Out of 48 bacterial isolates, 76.1% were gram-positive and 23.8% were Gram-negative bacteria. The overall drug resistance level of gram-positive bacteria was high against penicillin, co-amoxiclav, and amoxicillin. Vancomycin and imipenem were the most active drugs against Gram-positive bacteria. High level of drug resistance of Gram-negative bacteria against co-amoxiclav. Ceftriaxone, cefotaxime, and amikacin were the most active drugs against Gram-negative bacteria. Conclusions: The prevalence of bacterial vaginosis was high. Routine culture of vaginal samples should be performed on patients with vaginitis and the drug susceptibility pattern of each isolate should be determined.

Correlation between high-risk HPV infection and bacterial vaginosis in gynecological outpatient department

Objective To explore the correlation between high-risk human papillomavirus (HPV) infection and bacterial vaginosis in gynecological outpatient department. Methods From January 2017 to June 2017, 1 563 cases of gynecologic outpatient who underwent vaginal microecological examination and HPV examination in Jiaxing Maternal and Child Health Care Center were selected to analyze the infection rate of high-risk HPV in different age groups.The 1 563 patients were divided into high-risk HPV positive group and negative group.The vaginal microecological indicators and the incidence of bacterial vaginosis in the two groups were analyzed. Results Among the 1 563 patients, 385 caes were positive for high-risk HPV, the positive rate was 24.63%.The positive rate of high-risk HPV infection increased with age.There wereno statistically significant differences in pH > 4.5, hydrogen peroxide and leukocyte esterase positive rate between high-risk HPV positive group and negative group (40.52% vs.59.48%, 59.74% vs.40.26%, 51.17% vs.48.83%)(χ2=1.625, 0.188, 3.61, all P>0.05), but there was statistically significant difference in sialidase positive rate (12.47% vs.87.53%)(χ2=39.015, P<0.05). The incidence rate of bacterial vaginosis and the positive rate of microecology in the high-risk HPV positive group were significantly higher than those in the negative group (12.73% vs.6.03%, 8.83% vs.3.65%)(χ2=18.377, 16.629, all P<0.05). Conclusion High-risk HPV infection is closely related to female bacterial vaginitis, so it is necessary to pay more attention to the treatment of bacterial vaginosis, restore the balance of vaginal microenvironment, and enhance the vaginal and cervical immunity to further reduce high-risk HPV infection. Key words: Human papillomavirus; Vaginosis, Bacterial; High-risk; Infection; Microenvironment

Effect of Bacterial Vaginozis and cervikal length on preterm delivery in second trimester

Objective: Preterm birth is one of the major cause of perinatal morbidity and mortality. Clinical studies have pointed out the association between preterm birth and bacterial vaginosis (BV) infection. Our aim is to discover the incidence of BV and search for the mean cervical length and parameters for the prediction of preterm delivery.&#x0D; Materials and Methods: 130 pregnant woman between the 16th and 24th gestational week were included in our study. A detailed medical history was obtained from all of the women, and patients with a history of preterm delivery and the Vaginal Ph values and cervical length measurement were evaluated. Vaginal samples were analyzed, gram staining was performed, and a bacterial vaginosis diagnosis was made with Nugent’s criteria.&#x0D; Results: Of 130 woman that we included in our study, only 19 had bacterial vaginosis (14.6 %), and the mean cervical length was measured as 41.79 mm. Preterm birth occurred in nine of the pregnant women (6.9%), and no statistically significant difference was found between BV and shortened cervical length or preterm labor. When we excluded the known preterm birth risk factors from our study, we could not find a significant difference between preterm labor and BV.&#x0D; Conclusion: We concluded that BV by itself is not a preterm risk factor. The frequency of BV in the pregnant women in our study group according to the preterm delivery rate and the mean cervical length were similar to those in international studies in which the relationship between cervical length and preterm delivery has been established. We did not come to a significant conclusion in our research; nevertheless, we can relate this result to the preterm delivery rate that is found to be lower than expected.

Vaginal biocenosis in pregnant women with recurrent bacterial vaginosis after treatment with recombinant human interferon alpha-2b

Introduction.Preventing recurrent bacterial vaginosis (BV) during pregnancy is an important issue in current obstetrics.Aim:to assess the effect of recombinant human interferon alpha-2b on the incidence of recurrent BV during pregnancy and on the bacterial, cytokine, and antimicrobial components of the nonspecific vaginal immunity.Materials and methods.The study included 80 pregnant women with recurrent BV, 40 of whom (the main group) were treated with recombinant human interferon alpha-2b in the first half of pregnancy and after 30 weeks of pregnancy in addition to standard therapy (metronidazole or clindamycin). The other 40 women (control group) did not receive this interferon drug. Patients of the both groups were examined for the rate of BV recurrence and also tested for their vaginal bacteria and levels of β-defensin-2 (HBD-2), interleukin-1β (IL-1β), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), and interferon-γ (INF-γ) in the third trimester of pregnancy.Results.Treatment with recombinant human interferon alfa-2b led to a 3-fold reduction in the occurrence of repeated episodes of BV and an increase in the content of lactobacilli (7.24 ± 0.33 and 7.03 ± 0.40 in patients with interferon therapy and without it, respectively; p = 0.013) mainly due to Lactobacillus crispatus (7.14 ± 0.69 in women who received interferon alfa-2b, compared with 6.36 ± 0.95 in patients on standard therapy; p = 0.003) and a decrease in the number of some types of anaerobes. Also, the use of interferon alpha-2b in pregnant women with recurrent BV led to an increase in the content of IL-1β by 2.5 times, IL-6 by 3.5 times, IL-10 by 1.5 times, INF-γ by 2 times, and HBD-2 by 4 times.Conclusion.Treatment of recurrent BV during pregnancy with recombinant human interferon alfa-2b, aimed at boosting the nonspecific vaginal immunity helps reduce the incidence of recurrent BV.